AAV capsids with increased tropism to brain tissue

ABSTRACT

The disclosure relates to compositions, methods, and processes for the preparation, use, and/or formulation of adeno-associated virus capsid proteins, wherein the capsid proteins comprise targeting peptide inserts for enhanced tropism to a target tissue.

RELATED APPLICATIONS

This application is a continuation of International Application No. PCT/US2021/025061, filed Mar. 31, 2021, which claims priority to U.S. Provisional Application No. 63/023,927 filed on May 13, 2020, and U.S. Provisional Application No. 63/122,300 filed on Dec. 7, 2020, the entire contents of each of which are hereby incorporated by reference in their entirety.

REFERENCE TO THE SEQUENCE LISTING

The present application is being filed along with a Sequence Listing in electronic format. The Sequence Listing is provided as a file entitled 2057_1119PCT_SL, created on Mar. 31, 2021 which is 7,087,883 bytes in size. The information in the electronic format of the sequence listing is incorporated herein by reference in its entirety.

FIELD OF THE DISCLOSURE

The disclosure relates to compositions, methods, and processes for the preparation, use, and/or formulation of adeno-associated virus capsid proteins, wherein the capsid proteins comprise peptides, e.g., targeting peptide inserts, for enhanced tropism to a target cell or tissue.

BACKGROUND

Gene delivery to the adult central nervous system (CNS) remains a significant challenge in gene therapy. Engineered adeno-associated virus (AAV) capsids with improved brain tropism represent an attractive solution to the limitations of CNS delivery.

AAV-derived vectors are promising tools for clinical gene transfer because of their non-pathogenic nature, their low immunogenic profile, low rate of integration into the host genome and long-term transgene expression in non-dividing cells. However, the transduction efficiency of AAV natural variants in certain organs is too low for clinical applications, and capsid neutralization by pre-existing neutralizing antibodies may prevent treatment of a large proportion of patients. For these reasons, considerable efforts have been devoted to obtaining novel capsid variants with enhanced properties. Of many approaches tested so far, significant advances have resulted from directed evolution of AAV capsids using in vitro or in vivo selection of capsid variants created by capsid sequence randomization using either error-prone PCR, shuffling of various parent serotypes, or insertion of fully randomized short peptides at defined positions.

Attempts at providing AAV capsids with improved properties, e.g., improved tropism to a target cell or tissue upon systemic administration, have met with limited success. As such, there is a need for improved methods of producing AAV capsids and resulting AAV capsids for delivery of a payload of interest to a target cell or tissue, e.g., a CNS cell or tissue, or a muscle cell or tissue.

SUMMARY OF THE DISCLOSURE

The present disclosure pertains at least in part, to compositions and methods for the production and use of an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant. In some embodiments, the AAV capsid variant has an enhanced tropism for a tissue or a cell, e.g., a CNS tissue, a CNS cell, a muscle tissue, or a muscle cell. Said tropism can be useful for delivery of a payload, e.g., a payload described herein to a cell or tissue, for the treatment of a disorder, e.g., a neurological or a neurodegenerative disorder, a muscular or a neuromuscular disorder, or a neuro-oncological disorder.

Accordingly, in one aspect, the present disclosure provides an AAV capsid polypeptide, e.g., an AAV capsid variant, comprising: the amino acid sequence of any of SEQ ID NO: 1725-3622 or 3648-3659; an amino acid sequence comprising no more than four modifications, e.g., substitutions, relative to the amino acid sequence of any of SEQ ID NO: 1725-3622 or 3648-3659; or at least 3, 4, 5, 6, 7, 8, or 9 consecutive amino acids from the amino acid sequence of any of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the amino acid sequence is present in loop VIII. In some embodiments, the amino acid sequence is present immediately subsequent to position 586, 588, or 589, relative to a reference sequence numbered according to the amino acid sequence of SEQ ID NO: 138. In some embodiments, the AAV capsid variant comprises the amino acid sequence of any one of SEQ ID NOs: 3636-3647, or an amino acid sequence with at least 80% (e.g., at least about 85, 90, 95, 96, 97, 98, or 99%) sequence identity thereto.

In another aspect, the present disclosure provides an AAV capsid polypeptide, e.g., an AAV capsid variant, comprising: a parental amino acid sequence having an insert, wherein the insert comprises the amino acid sequence of any of SEQ ID NO: 1725-3622 or 3648-3659; an amino acid sequence comprising no more than four modifications, e.g., substitutions, relative to the amino acid sequence of any of SEQ ID NO: 1725-3622 or 3648-3659; or at least 3, 4, 5, 6, 7, 8, or 9 consecutive amino acids from the amino acid sequence of any of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the parental sequence comprises an amino acid sequence comprising at least one, two, or three modifications but no more than 30, 20 or 10 modifications, e.g., substitutions, to the amino acid sequence of SEQ ID NO: 138; and/or the amino acid sequence of SEQ ID NO: 138, or an amino acid sequence substantially identical (e.g., having at least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence identity) thereto. In some embodiments, the insert is present in, e.g., inserted into, loop VIII of the parental amino acid sequence. In some embodiments, the insert is present, e.g., inserted, immediately subsequent to position 586, 588, or 589 of the parental sequence. In some embodiments, the AAV capsid variant further comprises a deletion at position 587 and/or a deletion at position 588 of the parental amino acid sequence.

In another aspect, the present disclosure provides a peptide, e.g., a targeting peptide, comprising: at least the amino acid sequence of any of SEQ ID NO: 1725-3622 or 3648-3659; an amino acid sequence comprising no more than four modifications, e.g., substitutions, relative to the amino acid sequence of any of SEQ ID NO: 1725-3622 or 3648-3659; or 3, 4, 5, 6, 7, 8, or 9 consecutive amino acids from the amino acid sequence of any of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide is encoded by the nucleotide sequence of any one of SEQ ID NOs: 3660-3671, or a nucleic acid sequence substantially identical (e.g., having at least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence identity) thereto; or a nucleotide sequence comprising at least one, two, three, four, five, six, or seven modifications but no more than ten modifications of the nucleotide sequences of any of SEQ ID NOs: 3660-3671. In some embodiments, the nucleotide sequence encoding the peptide comprises the nucleotide sequence of any one of SEQ ID NOs: 3660-3671, or a nucleic acid sequence substantially identical (e.g., having at least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence identity) thereto, or a nucleotide sequence comprising at least one, two, three, four, five, six, or seven modifications but no more than ten modifications of the nucleotide sequences of any of SEQ ID NOs: 3660-3671.

In yet another aspect, the present disclosure provides a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, wherein the AAV capsid variant comprises the amino acid sequence of any of SEQ ID NO: 1725-3622 or 3648-3659; an amino acid sequence comprising no more than four modifications, e.g., substitutions, relative to the amino acid sequence of any of SEQ ID NO: 1725-3622 or 3648-3659; or at least 3, 4, 5, 6, 7, 8, or 9 consecutive amino acids from the amino acid sequence of any of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the polynucleotide comprises the nucleotide sequence of any one of SEQ ID NOs: 3623-3635, or a nucleotide sequence with at least 80% (e.g., at least about 85, 90, 95, 96, 97, 98, or 99%) sequence identity thereto.

In yet another aspect, the present disclosure provides an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein. In some embodiments, the AAV particle comprises a nucleic acid sequence encoding a payload. In some embodiments, the AAV particle further comprises a viral genome comprising a promoter operably linked to the nucleic acid encoding the payload.

In yet another aspect, the present disclosure provides a method of making an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein. The method comprises providing a host cell comprising a viral genome and incubating the host cell under conditions suitable to enclose the viral genome in the AAV capsid variant, e.g., an AAV capsid variant described herein, thereby making the AAV particle.

In yet another aspect, the present disclosure provides a method of delivering a payload to a cell or tissue (e.g., a CNS cell, a CNS tissue, a muscle cell, or a muscle tissue). The method comprising administering an effective amount of an AAV particle comprising an AAV capsid variant described herein.

In yet another aspect, the present disclosure provides a method of treating a subject having or diagnosed with having neurological, e.g., a neurodegenerative, disorder. The method comprising administering an effective amount of an AAV particle comprising an AAV capsid variant described herein.

In yet another aspect, the present disclosure provides a method of treating a subject having or diagnosed with having a muscular disorder or a neuromuscular disorder. The method comprising administering an effective amount of an AAV particle comprising an AAV capsid variant described herein.

In yet another aspect, the present disclosure provides a method of treating a subject having or diagnosed with having a neuro-oncological disorder. The method comprising administering an effective amount of an AAV particle comprising an AAV capsid variant described herein.

The present disclosure presents an AAV capsid protein comprising a parental amino acid sequence selected from any of SEQ ID NO: 1-1724, which may have inserted therein at least one peptide selected from any member of SEQ ID NO: 1725-3622.

In certain embodiments, the inserted peptide is selected from SEQ ID NO: 1725-3622 and is inserted into the parental amino acid sequence.

In certain embodiments, the peptide is inserted at any amino acid position between amino acids 586-592, inclusive of the parental amino acid sequence. In such embodiments, the peptide may be inserted between amino acids 588-589 of the parental amino acid sequence.

In some embodiments, the parental amino acid sequence may be SEQ ID NO: 138 or SEQ ID NO: 11.

The present disclosure also presents a peptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 1725-3622.

In other aspects, the disclosure presents an AAV particle comprising an AAV capsid protein disclosed herein and a viral genome. In such aspects, the viral genome may comprise a nucleic acid sequence that encodes a payload. In some embodiments, the payload may be an RNAi agent, which may consist of dsRNA, siRNA, shRNA, pre-miRNA, pri-miRNA, miRNA, miRNA precursor, stRNA, lncRNA, piRNA, or snoRNA.

In additional aspects, the payload may be a peptide, polypeptide, antibody or antibody fragment.

The present disclosure also presents a pharmaceutical composition comprising the AAV particle and a pharmaceutically acceptable excipient, as well as a method of treating a disease in a subject by administering the pharmaceutical composition said subject.

Those skilled in the art will recognize or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following enumerated embodiments.

ENUMERATED EMBODIMENTS

-   -   1. An AAV capsid polypeptide, e.g., an AAV capsid variant,         comprising:         -   (a) the amino acid sequence of any of SEQ ID NO: 1725-3622             or 3648-3659;         -   (b) at least 3, 4, 5, 6, 7, 8, or 9 consecutive amino acids             from the amino acid sequence of any of SEQ ID NO: 1725-3622             or 3648-3659; or         -   (c) an amino acid sequence comprising no more than four             modifications, e.g., substitutions, relative to the amino             acid sequence of any of SEQ ID NO: 1725-3622 or 3648-3659.     -   2. An AAV capsid polypeptide, e.g., an AAV capsid variant,         comprising:         -   (a) the amino acid sequence of any of SEQ ID NO: 3648-3659;         -   (b) at least 3, 4, 5, 6, 7, 8, or 9 consecutive amino acids             from the amino acid sequence of any of SEQ ID NO: 3648-3659;             or         -   (c) an amino acid sequence comprising no more than four             modifications, e.g., substitutions, relative to the amino             acid sequence of any of SEQ ID NO: 3648-3659.     -   3. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         embodiment 1 or 2, which comprises at least 3, 4, 5, 6, 7, 8, or         9 consecutive amino acids from the amino acid sequence of any of         SEQ ID NO: 3648-3659.     -   4. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3, wherein the 3 consecutive amino         acids comprise PLN.     -   5. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-4, wherein the 4 consecutive amino         acids comprise PLNG (SEQ ID NO: 3678).     -   6. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of the preceding embodiments, wherein the 5 consecutive         amino acids comprise PLNGA (SEQ ID NO: 3679).     -   7. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of the preceding embodiments, wherein the 6 consecutive         amino acids comprise PLNGAV (SEQ ID NO: 3680).     -   8. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of the preceding embodiments, wherein the 7 consecutive         amino acids comprise PLNGAVH (SEQ ID NO: 3681).     -   9. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of the preceding embodiments, wherein the 8 consecutive         amino acids comprise PLNGAVHL (SEQ ID NO: 3682).     -   10. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of the preceding embodiments, wherein the 9 consecutive         amino acids comprise PLNGAVHLY (SEQ ID NO: 3648).     -   11. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3, wherein the 3 consecutive amino         acids comprise YST.     -   12. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3 or 11, wherein the 4 consecutive         amino acids comprise YSTD (SEQ ID NO: 3690).     -   13. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3 or 11-12, wherein the 5 consecutive         amino acids comprise YSTDE (SEQ ID NO: 3691) or YSTDV (SEQ ID         NO: 3700).     -   14. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3 or 11-13, wherein the 6 consecutive         amino acids comprise YSTDER (SEQ ID NO: 3692) or YSTDVR (SEQ ID         NO: 3701).     -   15. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3, wherein the 3 consecutive amino         acids comprise IVM.     -   16. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3 or 15, wherein the 4 consecutive         amino acids comprise IVMN (SEQ ID NO: 3693).     -   17. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3 or 15-16, wherein the 5 consecutive         amino acids comprise IVMNS (SEQ ID NO: 3694).     -   18. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3 or 15-17, wherein the 6 consecutive         amino acids comprise IVMNSL (SEQ ID NO: 3695).     -   19. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3 or 15-18, wherein the 7 consecutive         amino acids comprise IVMNSLK (SEQ ID NO: 3651).     -   20. The AAV capsid polypeptide, e.g., the AAV capsid variant of         any one of embodiments 1-19, which comprises an amino acid         sequence comprising at least one, two, or three but no more than         four modifications, e.g., substitutions, relative to the amino         acid sequence of any of SEQ ID NO: 3648-3659.     -   21. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-10 or 20, comprising the amino acid         sequence of PLNGAVHLY (SEQ ID NO: 3648), or an amino acid         sequence having at least one, two, or three but no more than         four modifications, e.g., substitutions, relative to the amino         acid sequence of PLNGAVHLY (SEQ ID NO: 3648), optionally,         wherein position 7 is H.     -   22. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3 or 20, comprising the amino acid         sequence of RDSPKGW (SEQ ID NO: 3649), or an amino acid sequence         having at least one, two, or three modifications but no more         than four modifications, e.g., substitutions, relative to the         amino acid sequence of RDSPKGW (SEQ ID NO: 3649).     -   23. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3 or 15-20, comprising the amino acid         sequence of IVMNSLK (SEQ ID NO: 3651), or an amino acid sequence         having at least one, two, or three modifications but no more         than four modifications, e.g., substitutions, relative to the         amino acid sequence of IVMNSLK (SEQ ID NO: 3651).     -   24. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3, 11-14, or 20, comprising the amino         acid sequence of YSTDVRM (SEQ ID NO: 3650), or an amino acid         sequence having at least one, two, or three modifications but no         more than four modifications, e.g., substitutions, relative to         the amino acid sequence of YSTDVRM (SEQ ID NO: 3650).     -   25. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3 or 20, comprising the amino acid         sequence of RESPRGL (SEQ ID NO: 3652), or a sequence having at         least one, two, or three modifications but no more than four         modifications, e.g., substitutions, relative to the amino acid         sequence of RESPRGL (SEQ ID NO: 3652).     -   26. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of the preceding embodiments, which comprises the amino         acid sequence of any of SEQ ID NO: 3648-3659.     -   27. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-26, comprising:         -   (i) an amino acid sequence encoded by the nucleotide             sequence of any one of SEQ ID NOs: 3660-3671, or a             nucleotide sequence substantially identical (e.g., having at             least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99%             sequence identity) thereto; or         -   (ii) an amino acid sequence encoded by a nucleotide sequence             comprising at least one, two, three, four, five, six, or             seven modifications but no more than ten modifications of             the nucleotide sequence of any of SEQ ID NOs: 3660-3671.     -   28. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-27, wherein the nucleotide sequence         encoding the AAV capsid variant comprises:         -   (i) the nucleotide sequence of any one of SEQ ID NOs:             3660-3671, or a nucleotide sequence substantially identical             (e.g., having at least 70%, 75%, 80%, 85%, 90%, 92%, 95%,             97%, 98%, or 99% sequence identity) thereto; or         -   (ii) a nucleotide sequence comprising at least one, two,             three, four, five, six, or seven modifications but no more             than ten modifications of the nucleotide sequence of any of             SEQ ID NOs: 3660-3671.     -   29. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-10, 20-21, or 26-28, comprising an         amino acid sequence encoded by:         -   (i) the nucleotide sequence of SEQ ID NO: 3660, or a             nucleotide sequence substantially identical (e.g., having at             least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99%             sequence identity) thereto; or         -   (ii) a nucleotide sequence comprising at least one, two,             three, four, five, six, or seven modifications but no more             than ten modifications of the nucleotide sequence of SEQ ID             NO: 3660.     -   30. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-10, 20-21, or 26-29, wherein the         nucleotide sequence encoding the AAV capsid variant comprises:         -   (i) the nucleotide sequence of SEQ ID NO: 3660, or a             nucleotide sequence substantially identical (e.g., having at             least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99%             sequence identity) thereto; or         -   (ii) a nucleotide sequence comprising at least one, two,             three, four, five, six, or seven modifications but no more             than ten modifications of the nucleotide sequence of SEQ ID             NO: 3660.     -   31. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3, 20, 22, or 26-28, comprising an         amino acid sequence encoded by:         -   (i) the nucleotide sequence of SEQ ID NO: 3661, or a             nucleotide sequence substantially identical (e.g., having at             least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99%             sequence identity) thereto; or         -   (ii) a nucleotide sequence comprising at least one, two,             three, four, five, six, or seven modifications but no more             than ten modifications of the nucleotide sequence of SEQ ID             NO: 3661.     -   32. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3, 20, 22, 26-28, or 31, wherein the         nucleotide sequence encoding the AAV capsid variant comprises:         -   (i) the nucleotide sequence of SEQ ID NO: 3661, or a             nucleotide sequence substantially identical (e.g., having at             least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99%             sequence identity) thereto; or         -   (ii) a nucleotide sequence comprising at least one, two,             three, four, five, six, or seven modifications but no more             than ten modifications of the nucleotide sequence of SEQ ID             NO: 3661.     -   33. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3, 11-14, 20, 24, or 26-28, comprising         an amino acid sequence encoded by:         -   (i) the nucleotide sequence of SEQ ID NO: 3662, or a             nucleotide sequence substantially identical (e.g., having at             least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99%             sequence identity) thereto; or         -   (ii) a nucleotide sequence comprising at least one, two,             three, four, five, six, or seven modifications but no more             than ten modifications of the nucleotide sequence of SEQ ID             NO: 3662.     -   34. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3, 11-14, 20, 24, 26-28, or 33, wherein         the nucleotide sequence encoding the AAV capsid variant         comprises:         -   (i) the nucleotide sequence of SEQ ID NO: 3662, or a             nucleotide sequence substantially identical (e.g., having at             least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99%             sequence identity) thereto; or         -   (ii) a nucleotide sequence comprising at least one, two,             three, four, five, six, or seven modifications but no more             than ten modifications of the nucleotide sequence of SEQ ID             NO: 3662.     -   35. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3, 15-20, 23, or 26-28, comprising an         amino acid sequence encoded by:         -   (i) the nucleotide sequence of SEQ ID NO: 3663, or a             nucleotide sequence substantially identical (e.g., having at             least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99%             sequence identity) thereto; or         -   (ii) a nucleotide sequence comprising at least one, two,             three, four, five, six, or seven modifications but no more             than ten modifications of the nucleotide sequence of SEQ ID             NO: 3663.     -   36. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3, 15-20, 23, 26-28, or 35, wherein the         nucleotide sequence encoding the AAV capsid variant comprises:         -   (i) the nucleotide sequence of SEQ ID NO: 3663, or a             nucleotide sequence substantially identical (e.g., having at             least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99%             sequence identity) thereto; or         -   (ii) a nucleotide sequence comprising at least one, two,             three, four, five, six, or seven modifications but no more             than ten modifications of the nucleotide sequence of SEQ ID             NO: 3663.     -   37. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3, 20, or 25-28, comprising an amino         acid sequence encoded by:         -   (i) the nucleotide sequence of SEQ ID NO: 3664, or a             nucleotide sequence substantially identical (e.g., having at             least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99%             sequence identity) thereto; or         -   (ii) a nucleotide sequence comprising at least one, two,             three, four, five, six, or seven modifications but no more             than ten modifications of the nucleotide sequence of SEQ ID             NO: 3664.     -   38. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3, 20, 25-28, or 37, wherein the         nucleotide encoding the AAV capsid variant comprises:         -   (i) the nucleotide sequence of SEQ ID NO: 3664, or a             nucleotide sequence substantially identical (e.g., having at             least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99%             sequence identity) thereto; or         -   (ii) a nucleotide sequence comprising at least one, two,             three, four, five, six, or seven modifications but no more             than ten modifications of the nucleotide sequence of SEQ ID             NO: 3664.     -   39. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of the preceding embodiments, wherein the amino acid         sequence is present in loop VIII.     -   40. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-10, 20-21, 26-30, or 39, wherein the         amino acid sequence is present immediately subsequent to         position 586, relative to a reference sequence numbered         according to the amino acid sequence of SEQ ID NO: 138.     -   41. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3, 15-20, 23, 26-28, 35-36, or 39,         wherein the amino acid sequence is present immediately         subsequent to position 588, relative to a reference sequence         numbered according to the amino acid sequence of SEQ ID NO: 138.     -   42. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3, 11-14, 20, 22, 24-28, 31-34, 37-38,         or 39 wherein the amino acid sequence is present immediately         subsequent to position 589, relative to a reference sequence         numbered according to the amino acid sequence of SEQ ID NO: 138.     -   43. The AAV capsid polypeptide, e.g., the AAV capsid variant of         any one of embodiments 1-42, which comprises an amino acid         residue other than “A” at position 587 and/or an amino acid         residue other than “Q” at position 588, numbered according to         SEQ ID NO: 138.     -   44. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-10, 20-21, 26-30, 39-40, or 43,         comprising the amino acid sequence of PLNGAVHLY (SEQ ID NO:         3648), wherein the amino acid sequence of PLNGAVHLY (SEQ ID         NO: 3648) is present immediately subsequent to position 586,         relative to a reference sequence numbered according to the amino         acid sequence of SEQ ID NO: 138.     -   45. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3, 20, 26-28, 39-40, or 43, comprising         the amino acid sequence of GGTLAVVSL (SEQ ID NO: 3654), wherein         the amino acid sequence of GGTLAVVSL (SEQ ID NO: 3654) is         present immediately subsequent to position 586, relative to a         reference sequence numbered according to the amino acid sequence         of SEQ ID NO: 138.     -   46. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3, 15-20, 23, 26-28, 35-36, 39, or 41,         comprising the amino acid sequence of IVMNSLK (SEQ ID NO: 3651),         wherein the amino acid sequence of IVMNSLK (SEQ ID NO: 3651) is         present immediately subsequent to position 588, relative to a         reference sequence numbered according to the amino acid sequence         of SEQ ID NO: 138.     -   47. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3, 11-14, 20, 22, 24-28, 31-34, 37-38,         39, or 42, comprising the amino acid sequence of any of SEQ ID         NOs: 3649, 3650, 3652, 3653, or 3655-3659, wherein the amino         acid sequence of any of the aforesaid sequences is present         immediately subsequent to position 589, relative to a reference         sequence numbered according to the amino acid sequence of SEQ ID         NO: 138.     -   48. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of the preceding embodiments, which further comprises         the amino acid substitution of K449R, numbered according to SEQ         ID NO: 138.     -   49. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of the preceding embodiments, which further comprises a         modification, e.g., an insertion, substitution, and/or deletion,         in loop I, II, IV and/or VI.     -   50. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of the preceding embodiments, which comprises an amino         acid sequence having at least one, two or three modifications         but not more than 30, 20 or 10 modifications of the amino acid         sequence of SEQ ID NO: 138.     -   51. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of the preceding embodiments, which comprises the amino         acid sequence of SEQ ID NO: 138, or an amino acid sequence with         at least 80% (e.g., at least about 85, 90, 95, 96, 97, 98, or         99%) sequence identity thereto.     -   52. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of the preceding embodiments, which comprises the amino         acid sequence of SEQ ID NO: 138.     -   53. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of the preceding embodiments, which comprises an amino         acid sequence encoded by the nucleotide sequence of SEQ ID NO:         137, or a sequence with at least 80% (e.g., at least about 85,         90, 95, 96, 97, 98, or 99%) sequence identity thereto.     -   54. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of the preceding embodiments, wherein the nucleotide         sequence encoding the capsid variant comprises the nucleotide         sequence of SEQ ID NO: 137, or a sequence with at least 80%         (e.g., at least about 85, 90, 95, 96, 97, 98, or 99%) sequence         identity thereto.     -   55. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of the preceding embodiments, which comprises a VP1         protein, a VP2 protein, a VP3 protein, or a combination thereof.     -   56. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of the preceding embodiments, which comprises the amino         acid sequence corresponding to positions 138-743, e.g., a VP2,         of any one of SEQ ID NOs: 3636-3647, or a sequence with at least         80% (e.g., at least about 85, 90, 95, 96, 97, 98, or 99%)         sequence identity thereto.     -   57. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of the preceding embodiments, which comprises the amino         acid sequence corresponding to positions 203-743, e.g., a VP3,         of any one of SEQ ID NOs: 3636-3647, or a sequence with at least         80% (e.g., at least about 85, 90, 95, 96, 97, 98, or 99%)         sequence identity thereto.     -   58. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of the preceding embodiments, which comprises the amino         acid sequence of any one of SEQ ID NOs: 3636-3647, or an amino         acid sequence with at least 80% (e.g., at least about 85, 90,         95, 96, 97, 98, or 99%) sequence identity thereto.     -   59. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of the preceding embodiments, which comprises an amino         acid sequence having at least one, two or three modifications,         but not more than 30, 20 or 10 modifications of the amino acid         sequence of any one of SEQ ID NOs: 3636-3647.     -   60. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of the preceding embodiments, which comprises an amino         acid sequence encoded by the nucleotide sequence of any one of         SEQ ID NOs: 3623-3635, or a nucleotide sequence with at least         80% (e.g., at least about 85, 90, 95, 96, 97, 98, or 99%)         sequence identity thereto.     -   61. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of the preceding embodiments, wherein the nucleotide         sequence encoding the capsid variant comprises the nucleotide         sequence of any one of SEQ ID NOs: 3623-3635, or a nucleotide         sequence with at least 80% (e.g., at least about 85, 90, 95, 96,         97, 98, or 99%) sequence identity thereto.     -   62. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-10, 20-21, 26-30, 39-40, 43-44, or         48-61, wherein the nucleotide sequence encoding the capsid         variant comprises the nucleotide sequence of SEQ ID NO: 3623, or         a nucleotide sequence with at least 80% (e.g., at least about         85, 90, 95, 96, 97, 98, or 99%) sequence identity thereto.     -   63. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 1-3, 15-20, 23, 26-28, 35-36, 39, 41, 46,         or 48-61, wherein the nucleotide sequence encoding the capsid         variant comprises the nucleotide sequence of SEQ ID NO: 3627, or         a nucleotide sequence with at least 80% (e.g., at least about         85, 90, 95, 96, 97, 98, or 99%) sequence identity thereto.     -   64. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of the preceding embodiments, wherein the nucleotide         sequence encoding the capsid variant is codon optimized.     -   65. An AAV capsid polypeptide, e.g., an AAV capsid variant,         comprising the amino acid sequence of any one of embodiments         1-10, 20-21, 26-30, 39-40, 43-44, 48-62, or 64, and further         comprising an amino acid sequence at least 95% identical to SEQ         ID NO: 3636.     -   66. An AAV capsid polypeptide, e.g., an AAV capsid variant,         comprising the amino acid sequence of SEQ ID NO: 3636.     -   67. An AAV capsid polypeptide, e.g., an AAV capsid variant,         comprising the amino acid sequence of any one of embodiments         1-3, 20, 22, 26-28, 31-32, 39, 42, 47-61, or 64, and further         comprising an amino acid sequence at least 95% identical to SEQ         ID NO: 3637.     -   68. An AAV capsid polypeptide, e.g., an AAV capsid variant,         comprising the amino acid sequence of SEQ ID NO: 3637.     -   69. An AAV capsid polypeptide, e.g., an AAV capsid variant,         comprising the amino acid sequence of any one of embodiments         1-3, 11-12, 20, 24, 26-28, 33-34, 39, 42, 47-61, or 64, and         further comprising an amino acid sequence at least 95% identical         to SEQ ID NO: 3638.     -   70. An AAV capsid polypeptide, e.g., an AAV capsid variant,         comprising the amino acid sequence of SEQ ID NO: 3638.     -   71. An AAV capsid polypeptide, e.g., an AAV capsid variant,         comprising the amino acid sequence of any one of embodiments         1-3, 15-20, 23, 26-28, 35-36, 39, 41, 46, 48-61, or 63-64, and         further comprising an amino acid sequence at least 95% identical         to SEQ ID NO: 3639.     -   72. An AAV capsid polypeptide, e.g., an AAV capsid variant,         comprising the amino acid sequence of SEQ ID NO: 3639.     -   73. An AAV capsid polypeptide, e.g., an AAV capsid variant,         comprising the amino acid sequence of any one of embodiments         1-3, 20, 25-28, 37-39, 42, 47-61, or 64, and further comprising         an amino acid sequence at least 95% identical to SEQ ID NO:         3640.     -   74. An AAV capsid polypeptide, e.g., an AAV capsid variant,         comprising the amino acid sequence of SEQ ID NO: 3640.     -   75. An AAV capsid polypeptide, e.g., an AAV capsid variant,         comprising the amino acid sequence of SEQ ID NO: 3641.     -   76. An AAV capsid polypeptide, e.g., an AAV capsid variant,         comprising the amino acid sequence of SEQ ID NO: 3642.     -   77. An AAV capsid polypeptide, e.g., an AAV capsid variant,         comprising the amino acid sequence of SEQ ID NO: 3643.     -   78. An AAV capsid polypeptide, e.g., an AAV capsid variant,         comprising the amino acid sequence of SEQ ID NO: 3644.     -   79. An AAV capsid polypeptide, e.g., an AAV capsid variant,         comprising the amino acid sequence of SEQ ID NO: 3645.     -   80. An AAV capsid polypeptide, e.g., an AAV capsid variant,         comprising the amino acid sequence of SEQ ID NO: 3646.     -   81. An AAV capsid polypeptide, e.g., an AAV capsid variant,         comprising the amino acid sequence of SEQ ID NO: 3647.     -   82. An AAV capsid polypeptide, e.g., an AAV capsid variant,         comprising an amino acid sequence encoded by the nucleotide         sequence of any one of SEQ ID NOs: 3623-3635, or a nucleotide         sequence at least 95% identical thereto.     -   83. An AAV capsid polypeptide, e.g., an AAV capsid variant,         comprising a parental amino acid sequence having an insert,         e.g., a targeting peptide, wherein the insert comprises:         -   (a) the amino acid sequence of any of SEQ ID NO: 1725-3622             or 3648-3659;         -   (b) at least 3, 4, 5, 6, 7, 8, or 9 consecutive amino acids             from the amino acid sequence of any of SEQ ID NO: 1725-3622             or 3648-3659; or         -   (c) an amino acid sequence comprising no more than four             modifications, e.g., substitutions, relative to the amino             acid sequence of any of SEQ ID NO: 1725-3622 or 3648-3659.     -   84. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         embodiment 83, wherein the parental sequence comprises:         -   (i) the amino acid sequence of SEQ ID NO: 138, or an amino             acid sequence substantially identical (e.g., having at least             70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence             identity) thereto; and/or         -   (ii) an amino acid sequence comprising at least one, two, or             three modifications but no more than 30, 20 or 10             modifications, e.g., substitutions, to the amino acid             sequence of SEQ ID NO: 138.     -   85. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         embodiment 83 or 84, wherein the parental sequence further         comprises a substitution at position K449, e.g., a K449R         substitution.     -   86. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 83-85, wherein the parental sequence         comprises an amino acid sequence encoded by the nucleotide         sequence of SEQ ID NO: 137, or a nucleotide sequence         substantially identical (e.g., having at least 70%, 75%, 80%,         85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence identity) thereto.     -   87. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 83-86, wherein the nucleotide sequence         encoding the parental sequence comprises the nucleotide sequence         of SEQ ID NO: 137, or a nucleotide sequence substantially         identical (e.g., having at least 70%, 75%, 80%, 85%, 90%, 92%,         95%, 97%, 98%, or 99% sequence identity) thereto.     -   88. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 83-87, wherein the parental sequence         comprises:         -   (i) the amino acid sequence of SEQ ID NO: 11 or an amino             acid sequence substantially identical (e.g., having at least             70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence             identity) thereto; and/or         -   (ii) an amino acid sequence comprising at least one, two, or             three modifications but no more than 30, 20 or 10             modifications, e.g., substitutions, to the amino acid             sequence of SEQ ID NO: 11;

optionally, provided that position 449 of SEQ ID NO: 11 is not K, e.g., is R.

-   -   89. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 83-88, wherein the insert comprises the         amino acid sequence of PLNGAVHLY (SEQ ID NO: 3648).     -   90. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 83-88, wherein the insert comprises the         amino acid sequence of GGTLAVVSL (SEQ ID NO: 3654).     -   91. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 83-88, wherein the insert comprises the         amino acid sequence of IVMNSLK (SEQ ID NO: 3651).     -   92. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 83-88, wherein the insert comprises an         amino acid sequence chosen from RDSPKGW (SEQ ID NO: 3649),         YSTDVRM (SEQ ID NO: 3650), RESPRGL (SEQ ID NO: 3652), SFNDTRA         (SEQ ID NO: 3653), YGLPKGP (SEQ ID NO: 3655) or STGTLRL (SEQ ID         NO: 3656).     -   93. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 83-92, wherein the insert is present in         loop VIII of the parental amino acid sequence.     -   94. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 83-90 or 93, wherein the insert is         present immediately subsequent to position 586 in the parental         amino acid sequence.     -   95. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 83-89 or 93-94, wherein the insert         comprises the amino acid sequence of PLNGAVHLY (SEQ ID NO: 3648)         and is inserted immediately subsequent to position 586 of the         parental amino acid sequence.     -   96. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 83-88, 90, or 93-94, wherein the insert         comprises the amino acid sequence of GGTLAVVSL (SEQ ID NO: 3654)         and is inserted immediately subsequent to position 586 of the         parental amino acid sequence.     -   97. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 83-90, 93-96, which comprises an amino         acid other than “A” at position 587 and/or an amino acid other         than “Q” at position 588 of the parental sequence.     -   98. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 83-90 or 93-97, further comprising a         deletion of amino acid “A” at position 587 and/or a deletion of         amino acid “Q” at position 588 of the parental amino acid         sequence.     -   99. The AAV capsid polypeptide, e.g., the AAV capsid variant, of         any one of embodiments 83-89, 93-95, or 97-98, comprising:         -   (i) an insert comprising the amino acid sequence of             PLNGAVHLY (SEQ ID NO: 3648), which is inserted immediately             subsequent to position 586 of the parental amino acid             sequence; and         -   (ii) a deletion of the amino acids “AQ” at positions 587 and             588 of the parental amino acid sequence.     -   100. The AAV capsid polypeptide, e.g., the AAV capsid variant,         of any one of embodiments 83-88, 90, 93-94, or 96-98,         comprising:         -   (i) an insert comprising the amino acid sequence of             GGTLAVVSL (SEQ ID NO: 3654), which is inserted immediately             subsequent to position 586 of the parental amino acid             sequence; and         -   (ii) a deletion of the amino acids “AQ” at positions 587 and             588 of the parental amino acid sequence.     -   101. The AAV capsid polypeptide, e.g., the AAV capsid variant,         of any one of embodiments 83-88, 91, or 93, wherein the insert         is inserted immediately subsequent to position 588 in the         parental amino acid sequence.         102. The AAV capsid polypeptide, e.g., the AAV capsid variant,         of any one of embodiments 83-88, 91, 93, or 101, wherein the         insert comprises the amino acid sequence of IVMNSLK (SEQ ID         NO: 3651) and is inserted immediately subsequent to position 588         of the parental amino acid sequence.     -   103. The AAV capsid polypeptide, e.g., the AAV capsid variant,         of embodiment 83-88, or 92-93, wherein the insert is inserted         immediately subsequent to position 589 in the parental amino         acid sequence.     -   104. The AAV capsid polypeptide, e.g., the AAV capsid variant,         of any one of embodiments 83-88, 92-93, or 103, wherein the         insert comprises an amino acid sequence chosen from RDSPKGW (SEQ         ID NO: 3649), YSTDVRM (SEQ ID NO: 3650), RESPRGL (SEQ ID NO:         3652), SFNDTRA (SEQ ID NO: 3653), YGLPKGP (SEQ ID NO: 3655) or         STGTLRL (SEQ ID NO: 3656) and is inserted immediately subsequent         to position 589 of the parental amino acid sequence.     -   105. The AAV capsid polypeptide, e.g., the AAV capsid variant,         of any one of the preceding embodiments, which does not comprise         an insert sequence present immediately subsequent to position         586, 588, or 589 numbered relative to SEQ ID NO: 138, having at         least 5 consecutive amino acids corresponding to positions 586         to 594 numbered relative to SEQ ID NO: 138, of any of SEQ ID         NOs: 1-1724, e.g., as described in Table 6.     -   106. The AAV capsid polypeptide, e.g., the AAV capsid variant,         of any one of the preceding embodiments, which does not comprise         the amino acid sequence of TLAVPFK (SEQ ID NO: 1262) present         immediately subsequent to position 588, numbered according to         SEQ ID NO: 138.     -   107. The AAV capsid polypeptide, e.g., the AAV capsid variant,         of any one of the preceding embodiments, which has an increased         tropism for a CNS cell or tissue, e.g., a brain cell, brain         tissue, spinal cord cell, or spinal cord tissue, relative to the         tropism of a reference sequence comprising the amino acid         sequence of SEQ ID NO: 138.     -   108. The AAV capsid polypeptide, e.g., the AAV capsid variant,         of any one of embodiments 1-12, 15-44, 46-74, 82-89, 91-95,         97-99, 101-107, which transduces a brain region, e.g., selected         from dentate nucleus, cerebellar cortex, cerebral cortex, brain         stem, hippocampus, thalamus and putamen, optionally wherein the         level of transduction is at least 5, 10, 50, 100, 200, 500,         1,000, 2,000, 5,000, or 10,000-fold greater as compared to a         reference sequence of SEQ ID NO: 138, e.g., when measured by an         assay, e.g., an immunohistochemistry assay, a qRT-PCR, or a         RT-ddPCR assay, e.g., as described in Example 5.     -   109. The AAV capsid polypeptide, e.g., the AAV capsid variant,         of any one of the preceding embodiments, which is enriched at         least about 5, 6, 7, 8, 9, or 10-fold, in the brain compared to         a reference sequence of SEQ ID NO: 138, e.g., when measured by         an assay as described in Example 4.     -   110. The AAV capsid polypeptide, e.g., the AAV capsid variant,         of any one of embodiments 1-14, 20-22, 24-34, 39-40, 42-44,         47-62, 64-70, 79-80, 82-89, 92-95, 97-99, or 103-109, which is         enriched at least about 20, 30, 40, or 50-fold in the brain         compared to a reference sequence of SEQ ID NO: 138, e.g., when         measured by an assay as described in Example 4.     -   111. The AAV capsid polypeptide, e.g., the AAV capsid variant,         of any one of embodiments 1-10, 20-22, 26-32, 39-40, 42-44,         47-62, 64-68, 82-89, 92-95, 97-99, or 103-110, which is enriched         at least about 100, 200, 300, or 400-fold in the brain compared         to a reference sequence of SEQ ID NO: 138, e.g., when measured         by an assay as described in Example 4.     -   112. The AAV capsid polypeptide, e.g., the AAV capsid variant,         of any one of embodiments 1-10, 20-21, 26-30, 39-40, 43-44,         48-62, 64-66, 82-89, 93-95, 97-99, or 105-111, which delivers an         increased level of viral genomes to a brain region, optionally         wherein the level of viral genomes is increased by at least 5,         10, 20, 30, 40 or 50-fold, as compared to a reference sequence         of SEQ ID NO: 138, e.g., when measured by an assay, e.g., a         qRT-PCR or a RT-ddPCR assay (e.g., as described in Example 5).     -   113. The AAV capsid polypeptide, e.g., the AAV capsid variant,         of any one of embodiments 1-12, 15-44, 46-74, 82-89, 91-95,         97-99, 101-112, which delivers an increased level of a payload         to a brain region, optionally wherein the level of the payload         is increased by at least 5, 10, 50, 100, 200, 500, 1,000, 2,000,         5,000, or 10,000-fold, as compared to a reference sequence of         SEQ ID NO: 138, e.g., when measured by an assay, e.g., a qRT-PCR         or a RT-ddPCR assay (e.g., as described in Example 5).     -   114. The AAV capsid polypeptide, e.g., the AAV capsid variant,         of embodiment 113, wherein the brain region comprises a frontal         cortex, sensory cortex, motor cortex, putamen, thalamus,         cerebellar cortex, dentate nucleus, caudate, and/or hippocampus.     -   115. The AAV capsid polypeptide, e.g., the AAV capsid variant,         of any one of embodiments 1-12, 15-44, 46-74, 82-89, 91-95,         97-99, 101-114, which delivers an increased level of a payload         to a spinal cord region, optionally wherein the level of the         payload is increased by at least 10, 20, 50, 100, 200, 300, 400,         500, 600, 700, 800 or 900-fold, as compared to a reference         sequence of SEQ ID NO: 138, e.g., when measured by an assay,         e.g., a qRT-PCR assay (e.g., as described in Example 5).     -   116. The AAV capsid polypeptide, e.g., the AAV capsid variant,         of embodiment 115, wherein the spinal cord region comprises a         cervical, thoracic, and/or lumbar region.     -   117. The AAV capsid polypeptide, e.g., the AAV capsid variant,         of any one of embodiments 1-3, 15-20, 23, 26-28, 35-36, 39, 41,         46, 48-61, 63-64, 71-72, 83-88, 91, 93, 101-102, 105-109, or         113-116, which shows preferential transduction in a brain region         relative to the transduction in the dorsal root ganglia (DRG).     -   118. The AAV capsid polypeptide, e.g., the AAV capsid variant,         of any one of embodiments 1-10, 20-21, 26-30, 39-40, 43-44,         48-62, 64-66, 82-89, 93-95, 97-99, or 105-116, wherein the         capsid variant:         -   (i) is enriched at least about 300, or 400-fold in the brain             compared to a reference sequence of SEQ ID NO: 138, e.g.,             when measured by an assay as described in Example 4;         -   (ii) transduces a brain region, e.g., selected from dentate             nucleus, cerebellar cortex, cerebral cortex, brain stem,             hippocampus, thalamus and putamen, wherein the level of             transduction is at least 500, 1,000, 2,000, 5,000, or             10,000-fold greater as compared to a reference sequence of             SEQ ID NO: 138, e.g., when measured by an assay, e.g., an             immunohistochemistry assay, a qRT-PCR, or a RT-ddPCR assay,             e.g., as described in Example 5;         -   (iii) delivers an increased level of a payload to a brain             region, optionally wherein the level of the payload is             increased by at least 500, 1,000, 2,000, 5,000, or             10,000-fold, as compared to a reference sequence of SEQ ID             NO: 138, e.g., when measured by an assay, e.g., a qRT-PCR or             a RT-ddPCR assay (e.g., as described in Example 5),             optionally wherein the brain region comprises a frontal             cortex, sensory cortex, motor cortex, putamen, thalamus,             cerebellar cortex, dentate nucleus, caudate, and/or             hippocampus;         -   (iv) delivers an increased level of a payload to a spinal             cord region, optionally wherein the level of the payload is             increased by at least 50, 100, 200, 300, 400, 500, 600, 700,             800 or 900-fold, as compared to a reference sequence of SEQ             ID NO: 138, e.g., when measured by an assay, e.g., a qRT-PCR             assay (e.g., as described in Example 5), optionally wherein             the spinal cord region comprises a cervical, thoracic,             and/or lumbar region; and/or         -   (v) delivers an increased level of viral genomes to a brain             region, optionally wherein the level of viral genomes is             increased by at least 5, 10, 20, 30, 40 or 50-fold, as             compared to a reference sequence of SEQ ID NO: 138, e.g.,             when measured by an assay, e.g., a qRT-PCR or a RT-ddPCR             assay (e.g., as described in Example 5), optionally wherein             the brain region comprises a frontal cortex, sensory cortex,             motor cortex, putamen, thalamus, cerebellar cortex, dentate             nucleus, caudate, and/or hippocampus.     -   119. An AAV capsid polypeptide, e.g., an AAV capsid variant         comprising: (a) the amino acid sequence of PLNGAVHLY (SEQ ID NO:         3648); (b) an amino acid sequence comprising at least one, two,         or three modifications but no more than four modifications,         e.g., substitutions, relative to the amino acid sequence of         PLNGAVHLY (SEQ ID NO: 3648); or (c) at least 3, 4, 5, 6, 7, 8,         or 9 consecutive amino acids from the amino acid sequence of         PLNGAVHLY (SEQ ID NO: 3648); and wherein the capsid variant:         -   (i) is enriched at least about 300 or 400-fold in the brain             compared to a reference sequence of SEQ ID NO: 138, e.g.,             when measured by an assay as described in Example 4;         -   (ii) transduces a brain region, e.g., selected from dentate             nucleus, cerebellar cortex, cerebral cortex, brain stem,             hippocampus, thalamus and putamen, wherein the level of             transduction is at least 500, 1,000, 2,000, 5,000, or             10,000-fold greater as compared to a reference sequence of             SEQ ID NO: 138, e.g., when measured by an assay, e.g., an             immunohistochemistry assay, a qRT-PCR, or a RT-ddPCR assay,             e.g., as described in Example 5;         -   (iii) delivers an increased level of a payload to a brain             region, optionally wherein the level of the payload is             increased by at least 500, 1,000, 2,000, 5,000, or             10,000-fold, as compared to a reference sequence of SEQ ID             NO: 138, e.g., when measured by an assay, e.g., a qRT-PCR or             a RT-ddPCR assay (e.g., as described in Example 5),             optionally wherein the brain region comprises a frontal             cortex, sensory cortex, motor cortex, putamen, thalamus,             cerebellar cortex, dentate nucleus, caudate, and/or             hippocampus;         -   (iv) delivers an increased level of a payload to a spinal             cord region, optionally wherein the level of the payload is             increased by at least 50, 100, 200, 300, 400, 500, 600, 700,             800 or 900-fold, as compared to a reference sequence of SEQ             ID NO: 138, e.g., when measured by an assay, e.g., a qRT-PCR             assay (e.g., as described in Example 5), optionally wherein             the spinal cord region comprises a cervical, thoracic,             and/or lumbar region; and/or         -   (v) delivers an increased level of viral genomes to a brain             region, optionally wherein the level of viral genomes is             increased by at least 5, 10, 20, 30, 40 or 50-fold, as             compared to a reference sequence of SEQ ID NO: 138, e.g.,             when measured by an assay, e.g., a qRT-PCR or a RT-ddPCR             assay (e.g., as described in Example 5), optionally wherein             the brain region comprises a frontal cortex, sensory cortex,             motor cortex, putamen, thalamus, cerebellar cortex, dentate             nucleus, caudate, and/or hippocampus.     -   120. The AAV capsid polypeptide, e.g., the AAV capsid variant,         of any one of embodiments 1-3, 15-20, 23, 26-28, 35-36, 39, 41,         46, 48-61, 63-64, 71-72, 83-88, 91, 93, 101-102, 105-109, or         113-117, wherein the AAV capsid variant has an increased tropism         for a muscle cell or tissue, e.g., a heart tissue, relative to         the tropism of a reference sequence comprising the amino acid         sequence of SEQ ID NO: 138.     -   121. The AAV capsid polypeptide, e.g., the AAV capsid variant,         of any one of embodiments 1-3, 15-20, 23, 26-28, 35-36, 39, 41,         46, 48-61, 63-64, 71-72, 83-88, 91, 93, 101-102, 105-109,         113-117, or 120, which delivers an increased level of a payload         to a muscle region, optionally wherein the level of the payload         is increased by at least 10, 15, 20, 30, or 40-fold, as compared         to a reference sequence of SEQ ID NO: 138, e.g., when measured         by an assay, e.g., an IHC assay or a RT-ddPCR assay (e.g., as         described in Example 5).     -   122. The AAV capsid polypeptide, e.g., the AAV capsid variant,         of embodiment 120 or 121, wherein the muscle region comprises a         heart muscle, quadriceps muscle, and/or a diaphragm muscle         region.     -   123. The AAV capsid polypeptide, e.g., the AAV capsid variant,         of any one of embodiments 120-122, wherein the muscle region         comprises a heart muscle region, e.g., a heart atrium muscle         region or a heart ventricle muscle region.     -   124. The AAV capsid polypeptide, e.g., the AAV capsid variant,         of any one of the preceding embodiments, which is isolated,         e.g., recombinant.     -   125. A polynucleotide encoding the polypeptide, e.g., the AAV         capsid variant of any one of embodiments 1-124.     -   126. The polynucleotide of embodiment 125, which comprises:         -   (i) a nucleotide sequence comprising at least one, two,             three, four, five, six, or seven modifications but no more             than ten modifications of the nucleotide sequences of any of             SEQ ID NOs: 3660-3671; or         -   (ii) the nucleotide sequence of any one of SEQ ID NOs:             3660-3671, or nucleotide sequence substantially identical             (e.g., having at least 70%, 75%, 80%, 85%, 90%, 92%, 95%,             97%, 98%, or 99% sequence identity) thereto.     -   127. The polynucleotide of embodiment 125 or 126, which         comprises the nucleotide sequence of any one of SEQ ID NOs:         3623-3635, or a nucleotide sequence with at least 80% (e.g., at         least about 85, 90, 95, 96, 97, 98, or 99%) sequence identity         thereto.     -   128. The polynucleotide of any one of embodiments 125-127, which         comprises a nucleotide sequence that is codon optimized.     -   129. A peptide, e.g., a targeting peptide, comprising:         -   (a) the amino acid sequence of any of SEQ ID NO: 1725-3622             or 3648-3659;         -   (b) an amino acid sequence comprising no more than four             modifications, e.g., substitutions, relative to the amino             acid sequence of any of SEQ ID NO: 1725-3622 or 3648-3659;             or         -   (c) at least 3, 4, 5, 6, 7, 8, or 9 consecutive amino acids             from the amino acid sequence of any of SEQ ID NO: 1725-3622             or 3648-3659.     -   130. A peptide, e.g., a targeting peptide, comprising the amino         acid sequence of any one of embodiments 1-106.     -   131. A peptide, e.g., a targeting peptide, comprising:         -   (i) the amino acid sequence of PLNGAVHLY (SEQ ID NO: 3648);         -   (ii) an amino acid sequence comprising at least one, two, or             three modifications but no more than four modifications,             e.g., substitutions, relative to the amino acid sequence of             PLNGAVHLY (SEQ ID NO: 3648); or         -   (iii) at least 3, 4, 5, 6, 7, 8, or 9 consecutive amino             acids from the amino acid sequence of PLNGAVHLY (SEQ ID NO:             3648).     -   132. A peptide, e.g., a targeting peptide, encoded by:         -   (i) the nucleotide sequence of SEQ ID NO: 3660 or a             nucleotide sequence substantially identical (e.g., having at             least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99%             sequence identity) thereto; or         -   (ii) a nucleotide sequence comprising at least one, two,             three, four, five, six, or seven modifications but no more             than ten modifications of the nucleotide sequence of SEQ ID             NO: 3660.     -   133. A peptide, e.g., a targeting peptide, wherein the         nucleotide sequence encoding the peptide comprises:         -   (i) the nucleotide sequence of SEQ ID NO: 3660 or a             nucleotide sequence substantially identical (e.g., having at             least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99%             sequence identity) thereto; or         -   (ii) a nucleotide sequence comprising at least one, two,             three, four, five, six, or seven modifications but no more             than ten modifications of the nucleotide sequence of SEQ ID             NO: 3660.     -   134. A peptide, e.g., a targeting peptide, comprising:         -   (i) the amino acid sequence of IVMNSLK (SEQ ID NO: 3651);         -   (ii) an amino acid sequence comprising at least one, two, or             three modifications but no more than four modifications,             e.g., substitutions, relative to the amino acid sequence of             IVMNSLK (SEQ ID NO: 3651); or         -   (iii) at least 3, 4, 5, 6, 7, 8, or 9 consecutive amino             acids from the amino acid sequence of IVMNSLK (SEQ ID NO:             3651).     -   135. A peptide, e.g., a targeting peptide, encoded by:         -   (i) the nucleotide sequence of SEQ ID NO: 3663, or a             nucleotide sequence substantially identical (e.g., having at             least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99%             sequence identity) thereto; or         -   (ii) a nucleotide sequence comprising at least one, two,             three, four, five, six, or seven modifications but no more             than ten modifications of the nucleotide sequence of SEQ ID             NO: 3663.     -   136. A peptide, e.g., a targeting peptide, wherein the         nucleotide sequence encoding the peptide comprises:         -   (i) the nucleotide sequence of SEQ ID NO: 3663, or a             nucleotide sequence substantially identical (e.g., having at             least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99%             sequence identity) thereto; or         -   (ii) a nucleotide sequence comprising at least one, two,             three, four, five, six, or seven modifications but no more             than ten modifications of the nucleotide sequence of SEQ ID             NO: 3663.     -   137. A peptide, e.g., a targeting peptide, comprising:         -   (i) the amino acid sequence of RDSPKGW (SEQ ID NO: 3649);         -   (ii) an amino acid sequence comprising at least one, two, or             three modifications but no more than four modifications,             e.g., substitutions, relative to the amino acid sequence of             RDSPKGW (SEQ ID NO: 3649); or         -   (iii) at least 3, 4, 5, 6, 7, 8, or 9 consecutive amino             acids from the amino acid sequence of RDSPKGW (SEQ ID NO:             3649).     -   138. A peptide, e.g., a targeting peptide, encoded by:         -   (i) the nucleotide sequence of SEQ ID NO: 3661, or a             nucleotide sequence substantially identical (e.g., having at             least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99%             sequence identity) thereto; or         -   (ii) a nucleotide sequence comprising at least one, two,             three, four, five, six, or seven modifications but no more             than ten modifications of the nucleotide sequence of SEQ ID             NO: 3661.     -   139. A peptide, e.g., a targeting peptide, wherein the         nucleotide sequence encoding the peptide comprises:         -   (i) the nucleotide sequence of SEQ ID NO: 3661, or a             nucleotide sequence substantially identical (e.g., having at             least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99%             sequence identity) thereto; or         -   (ii) a nucleotide sequence comprising at least one, two,             three, four, five, six, or seven modifications but no more             than ten modifications of the nucleotide sequence of SEQ ID             NO: 3661.     -   140. A peptide, e.g., a targeting peptide, comprising:         -   (i) the amino acid sequence of YSTDVRM (SEQ ID NO: 3650);         -   (ii) an amino acid sequence comprising at least one, two, or             three modifications but no more than four modifications,             e.g., substitutions, relative to the amino acid sequence of             YSTDVRM (SEQ ID NO: 3650); or         -   (iii) at least 3, 4, 5, 6, 7, 8, or 9 consecutive amino             acids from the amino acid sequence of YSTDVRM (SEQ ID NO:             3650).     -   141. A peptide, e.g., a targeting peptide, encoded by:         -   (i) the nucleotide sequence of SEQ ID NO: 3662, or a             nucleotide sequence substantially identical (e.g., having at             least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99%             sequence identity) thereto; or         -   (ii) a nucleotide sequence comprising at least one, two,             three, four, five, six, or seven modifications but no more             than ten modifications of the nucleotide sequence of SEQ ID             NO: 3662.     -   142. A peptide, e.g., a targeting peptide, wherein the         nucleotide sequence encoding the peptide comprises:         -   (i) the nucleotide sequence of SEQ ID NO: 3662, or a             nucleotide sequence substantially identical (e.g., having at             least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99%             sequence identity) thereto; or         -   (ii) a nucleotide sequence comprising at least one, two,             three, four, five, six, or seven modifications but no more             than ten modifications of the nucleotide sequence of SEQ ID             NO: 3662.     -   143. A peptide, e.g., a targeting peptide, comprising:         -   (i) the amino acid sequence of RESPRGL (SEQ ID NO: 3652);         -   (ii) an amino acid sequence comprising at least one, two, or             three modifications but no more than four modifications,             e.g., substitutions, relative to the amino acid sequence of             RESPRGL (SEQ ID NO: 3652); or         -   (iii) at least 3, 4, 5, 6, 7, 8, or 9 consecutive amino             acids from the amino acid sequence of RESPRGL (SEQ ID NO:             3652).     -   144. A peptide, e.g., a targeting peptide, encoded by:         -   (i) the nucleotide sequence of SEQ ID NO: 3664, or a             nucleotide sequence substantially identical (e.g., having at             least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99%             sequence identity) thereto; or         -   (ii) a nucleotide sequence comprising at least one, two,             three, four, five, six, or seven modifications but no more             than ten modifications of the nucleotide sequence of SEQ ID             NO: 3664.     -   145. A peptide, e.g., a targeting peptide, wherein the         nucleotide sequence encoding the peptide comprises:         -   (i) the nucleotide sequence of SEQ ID NO: 3664, or a             nucleotide sequence substantially identical (e.g., having at             least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99%             sequence identity) thereto; or         -   (ii) a nucleotide sequence comprising at least one, two,             three, four, five, six, or seven modifications but no more             than ten modifications of the nucleotide sequence of SEQ ID             NO: 3664.     -   146. An AAV capsid polypeptide, e.g., an AAV capsid variant,         comprising the peptide, e.g., targeting peptide, of any one of         embodiments 129-145.     -   147. A polynucleotide encoding the peptide, e.g., targeting         peptide, of any one of embodiments 129-145.     -   148. A polynucleotide encoding an AAV capsid polypeptide, e.g.,         an AAV capsid variant comprising:         -   (a) the amino acid sequence of any of SEQ ID NO: 1725-3622             or 3648-3659;         -   (b) an amino acid sequence comprising no more than four             modifications, e.g., substitutions, relative to the amino             acid sequence of any of SEQ ID NO: 1725-3622 or 3648-3659;             or         -   (c) at least 3, 4, 5, 6, 7, 8, or 9 consecutive amino acids             from the amino acid sequence of any of SEQ ID NO: 1725-3622             or 3648-3659;

optionally wherein the amino acid sequence of (a), (b), and/or (c) is present immediately subsequent to position 586, 588, or 589, relative to a reference sequence numbered according to the amino acid sequence of SEQ ID NO: 138.

-   -   149. A polynucleotide encoding an AAV capsid polypeptide, e.g.,         an AAV capsid variant, wherein the AAV capsid variant comprises:         -   (i) the amino acid sequence of PLNGAVHLY (SEQ ID NO: 3648);         -   (ii) an amino acid sequence comprising at least one, two, or             three modifications but no more than four modifications,             e.g., substitutions, relative to the amino acid sequence of             PLNGAVHLY (SEQ ID NO: 3648); or         -   (iii) at least 3, 4, 5, 6, 7, 8, or 9 consecutive amino             acids from the amino acid sequence of PLNGAVHLY (SEQ ID NO:             3648);

optionally wherein the amino acid sequence of (i), (ii), and/or (iii) is present immediately subsequent to position 586, relative to a reference sequence numbered according to the amino acid sequence of SEQ ID NO: 138.

-   -   150. The polynucleotide of embodiment 149, which comprises:         -   (i) the nucleotide sequence of SEQ ID NO: 3660 or a             nucleotide sequence substantially identical (e.g., having at             least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99%             sequence identity) thereto; or         -   (ii) a nucleotide sequence comprising at least one, two,             three, four, five, six, or seven modifications but no more             than ten modifications of the nucleotide sequence of SEQ ID             NO: 3660.     -   151. A polynucleotide encoding an AAV capsid polypeptide, e.g.,         an AAV capsid variant, wherein the AAV capsid variant comprises:         -   (i) the amino acid sequence of IVMNSLK (SEQ ID NO: 3651);         -   (ii) an amino acid sequence comprising at least one, two, or             three modifications but no more than four modifications,             e.g., substitutions, relative to the amino acid sequence of             IVMNSLK (SEQ ID NO: 3651); or         -   (iii) at least 3, 4, 5, 6, 7, 8, or 9 consecutive amino             acids from the amino acid sequence of IVMNSLK (SEQ ID NO:             3651);

optionally wherein the amino acid sequence of (i), (ii), and/or (iii) is present immediately subsequent to position 588, relative to a reference sequence numbered according to the amino acid sequence of SEQ ID NO: 138.

-   -   152. The polynucleotide of embodiment 151, which comprises:         -   (i) the nucleotide sequence of SEQ ID NO: 3663, or a             nucleotide sequence substantially identical (e.g., having at             least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99%             sequence identity) thereto; or         -   (ii) a nucleotide sequence comprising at least one, two,             three, four, five, six, or seven modifications but no more             than ten modifications of the nucleotide sequence of SEQ ID             NO: 3663.     -   153. A polynucleotide encoding an AAV capsid polypeptide, e.g.,         an AAV capsid variant, wherein the AAV capsid variant comprises:         -   (i) the amino acid sequence of RDSPKGW (SEQ ID NO: 3649);         -   (ii) an amino acid sequence comprising at least one, two, or             three modifications but no more than four modifications,             e.g., substitutions, relative to the amino acid sequence of             RDSPKGW (SEQ ID NO: 3649); or         -   (iii) at least 3, 4, 5, 6, 7, 8, or 9 consecutive amino             acids from the amino acid sequence of RDSPKGW (SEQ ID NO:             3649);

optionally wherein the amino acid sequence of (i), (ii), and/or (iii) is present immediately subsequent to position 589, relative to a reference sequence numbered according to the amino acid sequence of SEQ ID NO: 138.

-   -   154. The polynucleotide of embodiment 153, which comprises:         -   (i) the nucleotide sequence of SEQ ID NO: 3661, or a             nucleotide sequence substantially identical (e.g., having at             least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99%             sequence identity) thereto; or         -   (ii) a nucleotide sequence comprising at least one, two,             three, four, five, six, or seven modifications but no more             than ten modifications of the nucleotide sequence of SEQ ID             NO: 3661.     -   155. A polynucleotide encoding an AAV capsid polypeptide, e.g.,         an AAV capsid variant, wherein the AAV capsid variant comprises:         -   (i) the amino acid sequence of YSTDVRM (SEQ ID NO: 3650);         -   (ii) an amino acid sequence comprising at least one, two, or             three modifications but no more than four modifications,             e.g., substitutions, relative to the amino acid sequence of             YSTDVRM (SEQ ID NO: 3650); or         -   (iii) at least 3, 4, 5, 6, 7, 8, or 9 consecutive amino             acids from the amino acid sequence of YSTDVRM (SEQ ID NO:             3650);

optionally wherein the amino acid sequence of (i), (ii), and/or (iii) is present immediately subsequent to position 589, relative to a reference sequence numbered according to the amino acid sequence of SEQ ID NO: 138.

-   -   156. The polynucleotide of embodiment 155, which comprises:         -   (i) the nucleotide sequence of SEQ ID NO: 3662, or a             nucleotide sequence substantially identical (e.g., having at             least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99%             sequence identity) thereto; or         -   (ii) a nucleotide sequence comprising at least one, two,             three, four, five, six, or seven modifications but no more             than ten modifications of the nucleotide sequence of SEQ ID             NO: 3662.     -   157. A polynucleotide encoding an AAV capsid polypeptide, e.g.,         an AAV capsid variant, wherein the AAV capsid variant comprises:         -   (i) the amino acid sequence of RESPRGL (SEQ ID NO: 3652);         -   (ii) an amino acid sequence comprising at least one, two, or             three modifications but no more than four modifications,             e.g., substitutions, relative to the amino acid sequence of             RESPRGL (SEQ ID NO: 3652); or         -   (iii) at least 3, 4, 5, 6, 7, 8, or 9 consecutive amino             acids from the amino acid sequence of RESPRGL (SEQ ID NO:             3652);

optionally wherein the amino acid sequence of (i), (ii), and/or (iii) is present immediately subsequent to position 589, relative to a reference sequence numbered according to the amino acid sequence of SEQ ID NO: 138.

-   -   158. The polynucleotide of embodiment 157, which comprises:         -   (i) the nucleotide sequence of SEQ ID NO: 3664, or a             nucleotide sequence substantially identical (e.g., having at             least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99%             sequence identity) thereto; or         -   (ii) a nucleotide sequence comprising at least one, two,             three, four, five, six, or seven modifications but no more             than ten modifications of the nucleotide sequence of SEQ ID             NO: 3664.     -   159. The polynucleotide of any one of embodiments 147-158,         wherein the AAV capsid variant comprises:         -   (i) the amino acid sequence of any one of SEQ ID NOs:             3636-3647, or an amino acid sequence with at least 80%             (e.g., at least about 85, 90, 95, 96, 97, 98, or 99%)             sequence identity thereto; or         -   (ii) an amino acid sequence having at least one, two or             three modifications but not more than 30, 20 or 10             modifications of the amino acid sequence of any one of SEQ             ID NOs: 3636-3647.     -   160. The polynucleotide of any one of embodiments 147-159,         comprising the nucleotide sequence of any one of SEQ ID NOs:         3623-3635, or a nucleotide sequence with at least 80% (e.g., at         least about 85, 90, 95, 96, 97, 98, or 99%) sequence identity         thereto.     -   161. The polynucleotide, peptide, or AAV capsid polypeptide,         e.g., AAV capsid variant, of any one of embodiments 125-160,         which is isolated, e.g., recombinant.     -   162. An AAV particle comprising the AAV capsid polypeptide,         e.g., the AAV capsid variant, of any one of embodiments 1-124 or         146.     -   163. The AAV particle of embodiment 162, which comprises a         nucleotide sequence encoding a payload.     -   164. The AAV particle of embodiment 163, wherein the encoded         payload comprises a therapeutic protein or functional variant         thereof; an antibody or antibody fragment; an enzyme; a         component of a gene editing system; an RNAi agent (e.g., a         dsRNA, siRNA, shRNA, pre-miRNA, pri-miRNA, miRNA, stRNA, lncRNA,         piRNA, or snoRNA); or a combination thereof.     -   165. The AAV particle of embodiment 164, wherein the therapeutic         protein or functional variant thereof, e.g., a recombinant         protein, is associated with (e.g., aberrantly expressed in) a         neurological or neurodegenerative disorder, a muscular or         neuromuscular disorder, or a neuro-oncological disorder.     -   166. The AAV particle of embodiment 164 or 165, the therapeutic         protein or functional variant thereof is chosen from         apolipoprotein E (APOE) (e.g., ApoE2, ApoE3 and/or ApoE4); human         survival of motor neuron (SMN) 1 or SMN2; glucocerebrosidase         (GBA1); aromatic L-amino acid decarboxylase (AADC);         aspartoacylase (ASPA); tripeptidyl peptidase I (CLN2);         beta-galactosidase (GLB1); N-sulphoglucosamine sulphohydrolase         (SGSH); N-acetyl-alpha-glucosaminidase (NAGLU); iduronate         2-sulfatase (IDS); intracellular cholesterol transporter (NPC1);         gigaxonin (GAN); or a combination thereof.     -   167. The AAV particle of embodiment 164, wherein the antibody or         antibody binding fragment binds to:         -   (i) a CNS related target, e.g. an antigen associated with a             neurological or neurodegenerative disorder, e.g., β-amyloid,             APOE, tau, SOD1, TDP-43, huntingtin (HTT), and/or synuclein;         -   (ii) a muscular or neuromuscular related target, e.g., an             antigen associated with a muscular or neuromuscular             disorder; or         -   (iii) a neuro-oncology related target, e.g., an antigen             associated with a neuro-oncological disorder, e.g., HER2, or             EGFR (e.g., EGFRvIII).     -   168. The AAV particle of embodiment 164, wherein the enzyme         comprises a meganuclease, a zinc finger nuclease, a TALEN, a         recombinase, integrase, a base editor, a Cas9, or a fragment         thereof.     -   169. The AAV particle of embodiment 164, wherein the component         of a gene editing system comprises one or more components of a         CRISPR-Cas system.     -   170. The AAV particle of embodiment 164 or 169, wherein the one         or more components of the CRISPR-Cas system comprises a Cas9,         e.g., a Cas9 ortholog or a Cpf1, and a single guide RNA (sgRNA),         optionally wherein:         -   (i) the sgRNA is located upstream (5′) of the cas9 enzyme;             or         -   (ii) the sgRNA is located downstream (3′) of the cas9             enzyme.     -   171. The AAV particle of embodiment 164, wherein the RNAi agent         (e.g., a dsRNA, siRNA, shRNA, pre-miRNA, pri-miRNA, miRNA,         stRNA, lncRNA, piRNA, or snoRNA), modulates, e.g., inhibits,         expression of, a CNS related gene, mRNA, and/or protein.     -   172. The AAV particle of embodiment 171, wherein the CNS related         gene is chosen from SOD1, MAPT, APOE, HTT, C9ORF72, TDP-43, APP,         BACE, SNCA, ATXN1, ATXN3, ATXN7, SCN1A-SCN5A, SCN8A-SCN11A, or a         combination thereof.     -   173. The AAV particle of any one of embodiments 162-172, which         comprises a viral genome comprising a promoter operably linked         to the nucleic acid sequence encoding the payload.     -   174. The AAV particle of embodiment 173, wherein the promoter is         chosen from human elongation factor 1α-subunit (EF1α),         cytomegalovirus (CMV) immediate-early enhancer and/or promoter,         chicken β-actin (CBA) and its derivative CAG, β glucuronidase         (GUSB), or ubiquitin C (UBC), neuron-specific enolase (NSE),         platelet-derived growth factor (PDGF), platelet-derived growth         factor B-chain (PDGF-β), intercellular adhesion molecule 2         (ICAM-2), synapsin (Syn), methyl-CpG binding protein 2 (MeCP2),         Ca2+/calmodulin-dependent protein kinase II (CaMKII),         metabotropic glutamate receptor 2 (mGluR2), neurofilament light         (NFL) or heavy (NFH), β-globin minigene nβ2, preproenkephalin         (PPE), enkephalin (Enk) and excitatory amino acid transporter 2         (EAAT2), glial fibrillary acidic protein (GFAP), myelin basic         protein (MBP), a cardiovascular promoter (e.g., αMHC, cTnT, and         CMV-MLC2k), a liver promoter (e.g., hAAT, TBG), a skeletal         muscle promoter (e.g., desmin, MCK, C512) or a fragment, e.g., a         truncation, or a functional variant thereof.     -   175. The AAV particle of any one of embodiments 173 or 174,         wherein the viral genome further comprises a polyA signal         sequence.     -   176. The AAV particle of any one of embodiments 173-175, wherein         the viral genome further comprises an inverted terminal repeat         (ITR) sequence.     -   177. The AAV particle of any one of embodiments 173-176, wherein         the viral genome comprises an ITR sequence positioned 5′         relative to the encoded payload.     -   178. The AAV particle of any one of embodiments 173-177, wherein         the viral genome comprises an ITR sequence positioned 3′         relative to the encoded payload.     -   179. The AAV particle of any one of embodiments 173-178, wherein         the viral genome comprises an ITR sequence positioned 5′         relative to the encoded payload and an ITR sequence positioned         3′ relative to the encoded payload.     -   180. The AAV particle of any one of embodiments 173-179, wherein         the viral genome further comprises an enhancer, a Kozak         sequence, an intron region, and/or an exon region.     -   181. The AAV particle of any one of embodiments 173-180, wherein         the viral genome further comprises a miR binding site, e.g., a         miR binding site that modulates, e.g., reduces, expression of         the payload encoded by the viral genome in a cell or tissue         where the corresponding miRNA is expressed.     -   182. The AAV particle of any one of embodiments 173-181, wherein         the viral genome comprises at least 1-5 copies of a miR binding         site, e.g., at least 1, 2, 3, 4, or 5 copies.     -   183. The AAV particle of any one of embodiments 173-182, wherein         the viral genome comprises at least 3 copies of a miR binding         site, optionally wherein all three copies comprise the same miR         binding site, or at least one, two, or all of the copies         comprise a different miR binding site.     -   184. The AAV particle of any one of embodiments 173-182, wherein         the viral genome comprises at least 4 copies of a miR binding         site, optionally wherein all four copies comprise the same miR         binding site, or at least one, two, three, or all of the copies         comprise a different miR binding site.     -   185. The AAV particle of any one of embodiments 181-184, wherein         the miR binding site comprises a miR122 binding site, a miR183         binding site, a miR-142-3p, or a combination thereof, optionally         wherein:         -   (i) the miR122 binding site comprises the nucleotide             sequence of SEQ ID NO: 3672, or a nucleotide sequence             substantially identical (e.g., having at least 70%, 75%,             80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence identity)             thereto; or a nucleotide sequence having at least one, two,             three, four, five, six, or seven modifications, but no more             than ten modifications of SEQ ID NO: 3672;         -   (ii) the miR183 binding site comprises the nucleotide             sequence of SEQ ID NO: 3675, or a nucleotide sequence             substantially identical (e.g., having at least 70%, 75%,             80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence identity)             thereto; or a nucleotide sequence having at least one, two,             three, four, five, six, or seven modifications, but no more             than ten modifications of SEQ ID NO: 3675; and/or         -   (iii) the miR-142-3p binding site comprises the nucleotide             sequence of SEQ ID NO: 3674, or a nucleotide sequence             substantially identical (e.g., having at least 70%, 75%,             80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence identity)             thereto; or a nucleotide sequence having at least one, two,             three, four, five, six, or seven modifications, but no more             than ten modifications of SEQ ID NO: 3674.     -   186. The AAV particle of any one of embodiments 173-185, wherein         the viral genome is single stranded.     -   187. The AAV particle of any one of embodiments 173-186, wherein         the viral genome further comprises a nucleotide sequence         encoding a Rep protein, e.g., a non-structural protein, wherein         the Rep protein comprises a Rep78 protein, a Rep68, Rep52         protein, and/or a Rep40 protein.     -   188. The AAV particle of embodiment 187, wherein the Rep78         protein, the Rep68 protein, the Rep52 protein, and/or the Rep40         protein are encoded by at least one Rep gene.     -   189. The AAV particle of any one of embodiments 173-188, wherein         the viral genome further comprises a nucleic acid sequence         encoding the AAV capsid variant of any one of embodiments 1-124         or 146.     -   190. The AAV particle of any one of embodiments, 162-189, which         is isolated, e.g., recombinant.     -   191. A polypeptide comprising a parental amino acid sequence of         any of SEQ ID NO: 1-1724, having inserted therein one or more         targeting peptide inserts, said targeting peptide inserts         comprising individually a contiguous amino acid sequence region         of 2-9 amino acids selected from any of the targeting peptides         of SEQ ID NO: 1725-3622.     -   192. The polypeptide of embodiment 191, wherein the polypeptide         has a first targeting peptide insert, said first targeting         peptide insert having a contiguous amino acid region of at least         5 amino acids selected from any of the targeting peptides of SEQ         ID NO: 1725-3622.     -   193. An AAV capsid comprising VP1, VP2 and VP3 proteins, wherein         each of said VP1, VP2 and VP3 proteins comprises a polypeptide         of any of those of embodiment 191 or 192.     -   194. The polypeptide of embodiment 191, wherein the parental         amino acid sequence is SEQ ID NO: 138.     -   195. The polypeptide of embodiment 194, wherein the parental         amino acid sequence has the substitution, K449R.     -   196. The polypeptide of embodiment 194 or 195, wherein said         first targeting peptide insert comprises the amino acid sequence         PLNGAVHLY (SEQ ID NO: 3648) which is inserted immediately after         amino acid 586 of the parental amino acid sequence.     -   197. The polypeptide of embodiment 196, further comprising a         deletion of the amino acids “AQ” at positions 587-588 of the         parental amino acid sequence.     -   198. The polypeptide of embodiment 194 or 195, wherein said         first targeting peptide insert comprises the amino acid sequence         GGTLAVVSL (SEQ ID NO: 3654) which is inserted immediately after         amino acid 586 of the parental amino acid sequence.     -   199. The polypeptide of embodiment 198, further comprising a         deletion of the amino acids “AQ” at positions 587-588 of the         parental amino acid sequence.     -   200. The polypeptide of embodiment 194 or 195, wherein said         first targeting peptide insert comprises a 7 amino acid sequence         selected from RDSPKGW (SEQ ID NO: 3649), YSTDVRM (SEQ ID NO:         3650), RESPRGL (SEQ ID NO: 3652), SFNDTRA (SEQ ID NO: 3653),         YGLPKGP (SEQ ID NO: 3655) or STGTLRL (SEQ ID NO: 3656) which is         inserted immediately after amino acid 589 of the parental amino         acid sequence.     -   201. The polypeptide of embodiment 194 or 195, wherein said         first targeting peptide insert comprises a 7 amino acid sequence         IVMNSLK (SEQ ID NO: 3651) which is inserted immediately after         amino acid 588 of the parental amino acid sequence.     -   202. The polypeptide of embodiment 191, wherein the parental         amino acid sequence is SEQ ID NO: 5.     -   203. The polypeptide of embodiment 202, wherein the parental         amino acid sequence has the substitution, K449R.     -   204. An AAV capsid comprising VP1, VP2 and VP3 proteins, wherein         each of said VP1, VP2 and VP3 proteins comprises a polypeptide         of any of those of embodiments 194-203.     -   205. A polynucleotide encoding the polypeptides or capsid         proteins of any of embodiments 191-204.     -   206. The polynucleotide of embodiment 205, which is DNA and the         DNA sequence is codon optimized.     -   207. An AAV particle comprising a capsid of any of embodiments         193 or 205 and a vector genome encoding a therapeutic payload.     -   208. The AAV particle of embodiment 207, wherein the therapeutic         payload is a gene of interest.     -   209. The AAV particle of embodiment 208, wherein the therapeutic         payload encodes a therapeutic RNA.     -   210. An AAV VP1 capsid selected from the group consisting of any         of SEQ ID NO: 3636-3647.     -   211. A polynucleotide encoding any of the AAV VP1 capsid         proteins of embodiment 210.     -   212. A vector comprising a polynucleotide encoding the AAV         capsid variant of any one of embodiments 1-124 or 146, the         polynucleotide of any one of embodiments 126-128, 147-161,         205-206, or 211, a polynucleotide encoding the peptide, e.g.,         targeting peptide, of any one of embodiments 129-145 or 161, or         a polynucleotide encoding the polypeptide of any one of         embodiments 191-192 or 194-203.     -   213. A cell, e.g., a host cell, comprising the AAV capsid         variant of any one of embodiments 1-124 or 146, the         polynucleotide of any one of embodiments 126-128, 147-161,         205-206, or 211, the peptide of any one of embodiments 129-145         or 161, the polypeptide of any one of embodiments 191-192 or         194-203, the AAV particle of any one of embodiments 162-190 or         207-209, or the vector of embodiment 212.     -   214. The cell of embodiment 213, wherein the cell is a mammalian         cell or an insect cell.     -   215. The cell of embodiment 213 or 214, wherein the cell is a         cell of a brain region or a spinal cord region, optionally a         cell of the frontal cortex, sensory cortex, motor cortex,         caudate, dentate nucleus, cerebellar cortex, cerebral cortex,         brain stem, hippocampus, thalamus, putamen, cervical spinal cord         region, thoracic spinal cord region, and/or lumbar spinal cord         region.     -   216. The cell of embodiment 213 or 214, wherein the cell is a         neuron, a sensory neuron, a motor neuron, an astrocyte, or a         muscle cell (e.g., a cell of the heart, diaphragm, or         quadriceps).     -   217. A method of making an AAV particle, comprising         -   (i) providing a host cell comprising a viral genome; and         -   (ii) incubating the host cell under conditions suitable to             enclose the viral genome in the AAV capsid variant of any             one of embodiments 1-124 or 146 or an AAV capsid variant             encoded by the polynucleotide of any one of embodiments             125-128 or 147-161;         -   thereby making the AAV particle.     -   218. The method of embodiment 217, further comprising, prior to         step (i), introducing a first nucleic acid molecule comprising         the viral genome into the host cell.     -   219. The method of embodiment 217 or 218, wherein the host cell         comprises a second nucleic acid encoding the capsid variant.     -   220. The method of any one of embodiments 217-219, wherein the         second nucleic acid molecule is introduced into the host cell         prior to, concurrently with, or after the first nucleic acid         molecule.     -   221. A pharmaceutical composition comprising the AAV particle of         any one of embodiments 162-190, an AAV particle comprising the         capsid variant of any one of embodiments 1-124 or 146, an AAV         particle comprising the peptide of any one of embodiments         129-145 or 161, or an AAV particle comprising the polypeptide of         any one of embodiments 191-192 or 194-203, and a         pharmaceutically acceptable excipient.     -   222. A method of delivering a payload to a cell or tissue (e.g.,         a CNS cell, a CNS tissue, a muscle cell, or a muscle tissue),         comprising administering an effective amount of the         pharmaceutical composition of embodiment 221, the AAV particle         of any one of embodiments 162-190, an AAV particle comprising         the capsid variant of any one of embodiments 1-124 or 146, an         AAV particle comprising the peptide of any one of embodiments         129-145 or 161, or an AAV particle comprising the polypeptide of         any one of embodiments 191-192 or 194-203.     -   223. The method of embodiment 222, wherein the cell is a cell of         a brain region or a spinal cord region, optionally a cell of the         frontal cortex, sensory cortex, motor cortex, caudate, dentate         nucleus, cerebellar cortex, cerebral cortex, brain stem,         hippocampus, thalamus, putamen, cervical spinal cord region,         thoracic spinal cord region, and/or lumbar spinal cord region.     -   224. The method of embodiment 222 or 223, wherein the cell is a         neuron, a sensory neuron, a motor neuron, an astrocyte, or a         muscle cell (e.g., a cell of the heart, diaphragm, or         quadriceps).     -   225. The method of any one of embodiments 222-224, wherein the         cell or tissue is within a subject.     -   226. The method of embodiment 225, wherein the subject has, has         been diagnosed with having, or is at risk of having a         neurological, e.g., a neurodegenerative disorder.     -   227. The method of embodiment 225, wherein the subject has, has         been diagnosed with having, or is at risk of having a muscular         disorder or a neuromuscular disorder.     -   228. The method of embodiment 225, wherein the subject has, has         been diagnosed with having, or is at risk of having a         neuro-oncological disorder.     -   229. A method of treating a subject having or diagnosed with         having a neurological disorder, e.g., a neurodegenerative         disorder, comprising administering to the subject an effective         amount of the pharmaceutical composition of embodiment 221, the         AAV particle of any one of embodiments 162-190, an AAV particle         comprising the capsid variant of any one of embodiments 1-124 or         146, an AAV particle comprising the peptide of any one of         embodiments 129-145 or 161, or an AAV particle comprising the         polypeptide of any one of embodiments 191-192 or 194-203.     -   230. A method of treating a subject having or diagnosed with         having a muscular disorder or a neuromuscular disorder,         comprising administering to the subject an effective amount of         the pharmaceutical composition of embodiment 221, the AAV         particle of any one of embodiments 162-190, an AAV particle         comprising the capsid variant of any one of embodiments 1-124 or         146, an AAV particle comprising the peptide of any one of         embodiments 129-145 or 161, or an AAV particle comprising the         polypeptide of any one of embodiments 191-192 or 194-203.     -   231. A method of treating a subject having or diagnosed with         having a neuro-oncological disorder, comprising administering to         the subject an effective amount of the pharmaceutical         composition of embodiment 221, the AAV particle of any one of         embodiments 162-190, an AAV particle comprising the capsid         variant of any one of embodiments 1-124 or 146, an AAV particle         comprising the peptide of any one of embodiments 129-145 or 161,         or an AAV particle comprising the polypeptide of any one of         embodiments 191-192 or 194-203.     -   232. The method of any one of embodiments 229-231, where         treating comprises prevention of progression of the disease or         disorder in the subject.     -   233. The method of embodiment 225-232, wherein the subject is a         human.     -   234. The method of any one of embodiments 225-233, wherein the         AAV particle is administered to the subject intramuscularly,         intravenously, intracerebrally, intrathecally,         intracerebroventricularly, via intraparenchymal administration,         or via intra-cisterna magna injection (ICM).     -   235. The method of any one of embodiments 225-233, wherein the         AAV particle is administered to the subject via focused         ultrasound (FUS), e.g., coupled with the intravenous         administration of microbubbles (FUS-MB), or MRI-guided FUS         coupled with intravenous administration.     -   236. The method of any one of embodiments 225-235, wherein the         AAV particle is administered to the subject intravenously.     -   237. The method of any one of embodiments 222-236, wherein         administration of the AAV particle results in a decreased         presence, level, and/or activity of a gene, mRNA, protein, or         combination thereof.     -   238. The method of any one of embodiments 222-148, wherein         administration of the AAV particle results in an increased         presence, level, and/or activity of a gene, mRNA, protein, or a         combination thereof.     -   239. The pharmaceutical composition of embodiment 221, the AAV         particle of any one of embodiments 162-190, an AAV particle         comprising the capsid variant of any one of embodiments 1-124 or         146, an AAV particle comprising the polypeptide of any one of         embodiments 129-145 or 161, or an AAV particle comprising the         polypeptide of any one of embodiments 191-192 or 194-203, for         use in a method of delivering a payload to a cell or tissue.     -   240. The pharmaceutical composition of embodiment 221, the AAV         particle of any one of embodiments 162-190, an AAV particle         comprising the capsid variant of any one of embodiments 1-124 or         196, an AAV particle comprising the polypeptide of any one of         embodiments 129-145 or 161, or an AAV particle comprising the         polypeptide of any one of embodiments 191-192 or 194-203, for         use in a method of treating a neurological disorder,         neurodegenerative, disorder, muscular disorder, neuromuscular         disorder, or a neuro-oncological disorder.     -   241. The pharmaceutical composition of embodiment 221, the AAV         particle of any one of embodiments 162-190, an AAV particle         comprising the capsid variant of any one of embodiments 1-124 or         146, an AAV particle comprising the peptide of any one of         embodiments 129-145 or 161, or an AAV particle comprising the         polypeptide of any one of embodiments 191-192 or 194-203, for         use in the manufacture of a medicament.     -   242. Use of the pharmaceutical composition of embodiment 221,         the AAV particle of any one of embodiments 162-190, an AAV         particle comprising the capsid variant of any one of embodiments         1-124 or 146, an AAV particle comprising the peptide of any one         of embodiments 129-145 or 161, or an AAV particle comprising the         polypeptide of any one of embodiments 191-192 or 194-203 in the         manufacture of a medicament for treating a neurological         disorder, a neurodegenerative disorder, a muscular disorder, a         neuromuscular disorder, or a neuro-oncological disorder.

BRIEF DESCRIPTION OF THE DRAWINGS

The foregoing and other objects, features, and advantages will be apparent from the following description of particular embodiments of the disclosure, as illustrated in the accompanying drawings. The drawings are not necessarily to scale, emphasis instead being placed upon illustrating the principles of various embodiments of the disclosure.

FIG. 1A and FIG. 1B provide diagrams of identification and design of non-human primate (NHP) TRACER AAV capsid libraries.

FIG. 2 provides a diagram of orthogonal evolution of TRACER AAV capsid libraries.

FIG. 3 provides a diagram of high-throughput screening by next-generation sequencing in non-human primate.

FIG. 4 provides a diagram of an exemplary TRACER AAV library design (SEQ ID NO: 3696-3699).

FIG. 5 provides a diagram of an alternative TRACER backbone construct.

FIG. 6A, FIG. 6B, and FIG. 6C show mRNA quantification of AAV particle transduction in NHP tissues using qRT-PCR.

FIG. 7 shows mRNA quantification of AAV particle transduction in NHP tissues using ddPCR.

FIG. 8 shows mRNA quantification of AAV particle transduction in the spinal cord and dorsal root ganglia of NHP.

FIG. 9A, FIG. 9B, FIG. 9C, and FIG. 9D show viral genome quantification in NHP tissues.

FIG. 10A and FIG. 10B show payload-HA quantification in peripheral tissues following AAV particle transduction as fold over TBP transcript (FIG. 10A) and as fold over AAV9 (FIG. 10B).

FIG. 11A and FIG. 11B show brain transgene mRNA expression (RT-ddPCR) as fold over TBP (housekeeping gene).

FIG. 12A and FIG. 12B show brain viral DNA biodistribution (ddPCR) as vector genomes per cell.

FIG. 13A and FIG. 13B show brain transgene mRNA expression (RT-ddPCR) as fold over AAV9.

FIG. 14A and FIG. 14B show brain viral DNA biodistribution (ddPCR) as fold over AAV9.

FIG. 15A and FIG. 15B show spinal cord (FIG. 15A) and DRG (FIG. 15B) transgene mRNA expression as fold over TATA box binding protein.

FIG. 16A and FIG. 16B show spinal cord (FIG. 16A) and DRG (FIG. 16B) viral genome biodistribution as vector genomes per cell.

FIG. 17A and FIG. 17B show spinal cord (FIG. 17A) and DRG (FIG. 17B) mRNA expression as fold over AAV9.

FIG. 18A and FIG. 18B show spinal cord (FIG. 18A) and DRG (FIG. 18B) viral genome biodistribution as fold over AAV9.

FIG. 19A, FIG. 19B, FIG. 19C, FIG. 19D, and FIG. 19E show images of the brain transduction profile for TTD-001 and AAV9 as determined by immunohistochemical analyses of the dentate nucleus (FIG. 19A), cerebellar cortex (FIG. 19B), cortex (FIG. 19C), brain stem, hippocampus, thalamus and putamen (FIG. 19D) and dorsal root ganglion (FIG. 19E).

FIG. 20A and FIG. 20B show immunohistochemistry images of the DRG de-targeting characteristic of capsid variant TTD-004, compared to AAV9.

FIG. 21A and FIG. 21B show viral genome biodistribution in peripheral tissues quantified as vector genomes per cell.

FIG. 22A, FIG. 22B, and FIG. 22C show immunohistochemistry images of the heart of a female NHP at day 14 post-intravenous administration of AAV particles comprising a TTD-001 capsid variant, a TTD-004 capsid variant, or a wild-type AAV9 control capsid polypeptide. FIG. 22A provides a series of global images of the heart muscle, FIG. 22B provides a series of images of the left ventricle of the heart, and FIG. 22C provides a series of images of the right ventricle of the heart. For each series of images in FIGS. 22A-22C, the top left panel shows staining following administration of AAV particles comprising a TTD-001 capsid variant, the top right panel shows staining following administration of AAV particles comprising a TTD-004 capsid variant, and the bottom panel shows staining following administration of AAV particles comprising a wild-type AAV9 control capsid variant.

DETAILED DESCRIPTION OF THE DISCLOSURE

The details of one or more embodiments of the disclosure are set forth in the accompanying description below. Although any materials and methods similar or equivalent to those described herein can be used in the practice or testing of the present disclosure, the preferred materials and methods are now described. Other features, objects and advantages of the disclosure will be apparent from the description. In the description, the singular forms also include the plural unless the context clearly dictates otherwise. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. In the case of conflict, the present description will control. Certain terms are defined in the Definition section and throughout.

Described herein, inter alia, are compositions comprising an AAV capsid polypeptide, e.g., an AAV capsid variant, e.g., an AAV capsid variant described herein, and methods of making and using the same. Generally, the AAV capsid variant has enhanced tropism for a cell or tissue, e.g., for the delivery of a payload to said cell or tissue, for example a CNS tissue, a CNS cell, a muscle cell, or a muscle tissue.

As demonstrated in the Examples herein below, certain AAV capsid variants described herein show multiple advantages over wild-type AAV9, including (i) increased penetrance through the blood brain barrier following intravenous administration, (ii) wider distribution throughout the multiple brain regions, e.g., frontal cortex, sensory cortex, motor cortex, putamen, thalamus, cerebellar cortex, dentate nucleus, caudate, and/or hippocampus, and/or (iii) elevated payload expression in multiple brain regions. Without wishing to be being bound by theory, it is believed that these advantages may be due, in part, to the dissemination of the AAV capsid variants through the brain vasculature. In some embodiments, the AAV capsids described herein enhance the delivery of a payload to multiple regions of the brain including for example, the frontal cortex, sensory cortex, motor cortex, putamen, thalamus, cerebellar cortex, dentate nucleus, caudate, and/or hippocampus.

According to the present disclosure, AAV particles with enhanced tropism for a target tissue (e.g., CNS) are provided, as well as associated processes for their targeting, preparation, formulation and use. Peptides, e.g., targeting peptides, and nucleic acid sequences encoding the peptides, e.g., targeting peptides, are also provided. These peptides, e.g., targeting peptides, may be inserted into an AAV capsid protein sequence to alter tropism to a particular cell-type, tissue, organ or organism, in vivo, ex vivo or in vitro.

Several approaches have been used previously to produce AAV capsids with enhanced tropism for a cell or tissue, e.g., a CNS cell or tissue. One approach used co-infection of cultured cells (Grimm et al., 2008, the contents of which are herein incorporated by reference in its entirety) or in situ animal tissue (Lisowski et al., 2014, the contents of which are herein incorporated by reference in its entirety) with adenovirus, in order to trigger exponential replication of infectious AAV DNA. Another approach involved the use of cell-specific CRE transgenic mice (Deverman et al., 2016. the contents of which are herein incorporated by reference in its entirety) allowing viral DNA recombination specifically in astrocytes, followed by recovery of CRE-recombined capsid variants. Both approaches have had limited success.

The transgenic CRE system used by Deverman et al. (2016) has limited tractable in other animal species and AAV variants selected by directed evolution in mouse tissue do not show similar properties in large animals Previously described transduction-specific approaches are not amenable to large animal studies because: 1) many tissues of interest (e.g. CNS) are not readily accessible to adenovirus co-infection, 2) the specific adenovirus tropism itself would bias the library distribution, and 3) large animals are typically not amenable to transgenesis or genetic engineering to express CRE recombinase in defined cell types.

To address these limitations, a broadly-applicable functional AAV capsid library screening platform for cell type-specific biopanning in non-transgenic animals has been developed and is described in the appended Examples. In the TRACER (Tropism Redirection of AAV by Cell type-specific Expression of RNA) platform system, the capsid gene is placed under the control of a cell type-specific promoter to drive capsid mRNA expression in the absence of helper virus co-infection. Without wishing to be bound by theory, it is believed that this RNA-driven screen increases the selective pressure in favor of capsid variants which transduce a specific cell type. The TRACER platform allows for generation of AAV capsid libraries whereby specific recovery and subcloning of capsid mRNA expressed in transduced cells is achieved with no need for transgenic animals or helper virus co-infection. Without wishing to be bound by theory, it is believed that since mRNA transcription is a hallmark of full transduction, the methods disclosed herein allow identification of fully infectious AAV capsid mutants, and in addition to its higher stringency, this method allows identification of capsids with high tropism for particular cell types using libraries designed to express CAP mRNA under the control of any cell-specific promoter such as, but not limited to, synapsin-1 promoter (neurons), GFAP promoter (astrocytes), TBG promoter (liver), CAMK promoter (skeletal muscle), MYH6 promoter (cardiomyocytes). Described herein are novel AAV capsid variants generated using the TRACER method which demonstrate enhance tropism in for example a CNS cell, a CNS tissue, a muscle cell, or a muscle tissue.

The AAV particles and payloads of the disclosure may be delivered to one or more target cells, tissues, organs, or organisms. In some embodiments, the AAV particles of the disclosure demonstrate enhanced tropism for a target cell type, tissue or organ. As a non-limiting example, the AAV particle may have enhanced tropism for cells and tissues of the central or peripheral nervous systems (CNS and PNS, respectively), or cells and tissues of a muscle. The AAV particles of the disclosure may, in addition, or alternatively, have decreased tropism for an undesired target cell-type, tissue or organ.

In some embodiments, an AAV comprises a small non-enveloped icosahedral capsid virus of the Parvoviridae family and is characterized by a single stranded DNA viral genome. Parvoviridae family viruses consist of two subfamilies: Parvovirinae, which infect vertebrates, and Densovirinae, which infect invertebrates. The Parvoviridae family comprises the Dependovirus genus which includes AAV, capable of replication in vertebrate hosts including, but not limited to, human, primate, bovine, canine, equine, and ovine species.

The parvoviruses and other members of the Parvoviridae family are generally described in Kenneth I. Berns, “Parvoviridae: The Viruses and Their Replication,” Chapter 69 in FIELDS VIROLOGY (3d Ed. 1996), the contents of which are incorporated by reference in their entirety.

In some embodiments, AAV are used as a biological tool due to a relatively simple structure, their ability to infect a wide range of cells (including quiescent and dividing cells) without integration into the host genome and without replicating, and their relatively benign immunogenic profile. The genome of the virus may be manipulated to contain a minimum of components for the assembly of a functional recombinant virus, or viral particle, which is loaded with or engineered to target a particular tissue and express or deliver a desired payload.

In some embodiments, the AAV, is a naturally occurring (e.g., wild-type) AAV or a recombinant AAV. In some embodiments, the wild-type AAV vector genome is a linear, single-stranded DNA (ssDNA) molecule approximately 5,000 nucleotides (nt) in length. In some embodiments, inverted terminal repeats (ITRs) cap the viral genome at both the 5′ and the 3′ end, providing origins of replication for the viral genome. In some embodiments, an AAV viral genome typically comprises two ITR sequences. These ITRs have a characteristic T-shaped hairpin structure defined by a self-complementary region (145nt in wild-type AAV) at the 5′ and 3′ ends of the ssDNA which form an energetically stable double stranded region. The double stranded hairpin structures comprise multiple functions including, but not limited to, acting as an origin for DNA replication by functioning as primers for the endogenous DNA polymerase complex of the host viral replication cell.

In some embodiments, the wild-type AAV viral genome further comprises nucleotide sequences for two open reading frames, one for the four non-structural Rep proteins (Rep78, Rep68, Rep52, Rep40, encoded by Rep genes) and one for the three capsid, or structural, proteins (VP1, VP2, VP3, encoded by capsid genes or Cap genes). The Rep proteins are used for replication and packaging, while the capsid proteins are assembled to create the protein shell of the AAV, or AAV capsid polypeptide, e.g., an AAV capsid variant. Alternative splicing and alternate initiation codons and promoters result in the generation of four different Rep proteins from a single open reading frame and the generation of three capsid proteins from a single open reading frame. Though it varies by AAV serotype, as a non-limiting example, for AAV9/hu.14 (SEQ ID NO: 123 of U.S. Pat. No. 7,906,111, the contents of which are herein incorporated by reference in their entirety) VP1 refers to amino acids 1-736, VP2 refers to amino acids 138-736, and VP3 refers to amino acids 203-736. In some embodiments, for any one of the amino acid sequences of SEQ ID NOs: 3636-3647, VP1 comprises amino acids 1-743, VP2 comprises amino acids 138-743, and VP3 comprises amino acids 203-743. In other words, VP1 is the full-length capsid sequence, while VP2 and VP3 are shorter components of the whole. As a result, changes in the sequence in the VP3 region, are also changes to VP1 and VP2, however, the percent difference as compared to the parent sequence will be greatest for VP3 since it is the shortest sequence of the three. Though described here in relation to the amino acid sequence, the nucleic acid sequence encoding these proteins can be similarly described. Together, the three capsid proteins assemble to create the AAV capsid protein. While not wishing to be bound by theory, the AAV capsid protein typically comprises a molar ratio of 1:1:10 of VP1:VP2:VP3.

AAV vectors of the present disclosure may be produced recombinantly and may be based on adeno-associated virus (AAV) parent or reference sequences. In addition to single stranded AAV viral genomes (e.g., ssAAVs), the present disclosure also provides for self-complementary AAV (scAAVs) viral genomes. scAAV vector genomes contain DNA strands which anneal together to form double stranded DNA. By skipping second strand synthesis, scAAVs allow for rapid expression in the transduced cell. In some embodiments, the AAV particle of the present disclosure is an scAAV. In some embodiments, the AAV particle of the present disclosure is an ssAAV.

Methods for producing and/or modifying AAV particles are disclosed in the art such as pseudotyped AAV vectors (PCT Patent Publication Nos. WO200028004; WO200123001; WO2004112727; WO2005005610; and WO2005072364, the content of each of which is incorporated herein by reference in its entirety).

As described herein, the AAV particles of the disclosure comprising an AAV capsid polypeptide, e.g., an AAV capsid variant, and a viral genome, have enhanced tropism for a cell-type or a tissue, e.g., a CNS cell-type, region, or tissue, or a muscle cell-type or tissue. In some embodiments, the AAV particles of the disclosure comprising a capsid with an inserted peptide, e.g., a targeting peptide, and a viral genome, may have enhanced tropism for a cell-type, region, or tissue of the human CNS or a muscle.

Peptides, e.g., Targeting Peptides

Disclosed herein are peptides, e.g., targeting peptides, and associated AAV particles comprising an AAV capsid polypeptide, e.g., an AAV capsid variant, with a peptide, e.g., targeting peptide insert, for enhanced or improved transduction of a target tissue (e.g., cells of the CNS or PNS). In some, embodiments, the peptide, e.g., targeting peptide, is an isolated, e.g., recombinant, peptide, e.g., targeting peptide. In some embodiments, the nucleic acid encoding the peptide, e.g., targeting peptide, is an isolated, e.g., recombinant nucleic acid.

In some embodiments, the peptide, e.g., targeting peptide, may direct an AAV particle to a cell, region, or tissue of the CNS. The cell of the CNS may be, but is not limited to, neurons (e.g., excitatory, inhibitory, motor, sensory, autonomic, sympathetic, parasympathetic, Purkinje, Betz, etc.), glial cells (e.g., microglia, astrocytes, oligodendrocytes) and/or supporting cells of the brain such as immune cells (e.g., T cells). The tissue of the CNS may be, but is not limited to, the cortex (e.g., frontal, parietal, occipital, temporal), thalamus, hypothalamus, striatum, putamen, caudate nucleus, hippocampus, entorhinal cortex, basal ganglia, or deep cerebellar nuclei.

In some embodiments, the peptide, e.g., targeting peptide, may direct an AAV particle to a cell, region, or tissue of the PNS. The cell or tissue of the PNS may be, but is not limited to, a dorsal root ganglion (DRG).

In some embodiments, the peptide, e.g. targeting peptide, may direct an AAV particle to the CNS (e.g., the cortex) after intravenous administration. In some embodiments, the peptide, e.g. targeting peptide, may direct an AAV particle to the CNS (e.g., the cortex) following focused ultrasound (FUS), e.g., coupled with the intravenous administration of microbubbles (FUS-MB), or MRI-guided FUS coupled with intravenous administration.

In some embodiments, the peptide, e.g., targeting peptide, may direct an AAV particle to the PNS (e.g., DRG) after intravenous administration. In some embodiments, the peptide, e.g., targeting peptide, may direct an AAV particle to the PNS (e.g., DRG) following focused ultrasound (FUS), e.g., coupled with the intravenous administration of microbubbles (FUS-MB), or MRI-guided FUS coupled with intravenous administration.

In some embodiments, the peptide, e.g., targeting peptide, may direct an AAV particle to a cell, region, or tissue of a muscle. In some embodiments, the muscle is a heart muscle. In some embodiments, the peptide, e.g., targeting peptide, may direct an AAV particle to a muscle cell, region, or tissue after intravenous administration.

A peptide, e.g., a targeting peptide, may vary in length. In some embodiments, the peptide, e.g., targeting peptide, is about 3 to about 20 amino acids in length. As non-limiting examples, the targeting peptide may be 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20 or 3-5, 3-8, 3-10, 3-12, 3-15, 3-18, 3-20, 5-10, 5-15, 5-20, 10-12, 10-15, 10-20, 12-20, or 15-20 amino acids in length. In some embodiments, a peptide comprises about 6 to 12 amino acids in length, e.g., about 9 amino acids in length. In some embodiments, a peptide comprises about 5 to 10 amino acids in length, e.g., about 7 amino acids in length.

A peptide, e.g., a targeting peptide, may be contiguous (or continuous) or noncontiguous (or not continuous), or split, or divided across two or more amino acid sequences by intervening amino acid sequences that may vary in length. The contiguous peptide, e.g., targeting peptide, may vary in length. As non-limiting examples, the contiguous peptide, e.g., targeting peptide, may be 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20 or 3-5, 3-8, 3-10, 3-12, 3-15, 3-18, 3-20, 5-10, 5-15, 5-20, 10-12, 10-15, 10-20, 12-20, or 15-20 amino acids in length. The noncontiguous, or split, peptide, e.g., targeting peptide, may vary in length. As non-limiting examples, the noncontiguous, or split, peptide, e.g., targeting peptide, may be 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20 or 3-5, 3-8, 3-10, 3-12, 3-15, 3-18, 3-20, 5-10, 5-15, 5-20, 10-12, 10-15, 10-20, 12-20, or 15-20 amino acids in length. The intervening amino acid sequence may vary in length. As non-limiting examples, the intervening peptide, e.g., targeting peptide, may be 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20 or 3-5, 3-8, 3-10, 3-12, 3-15, 3-18, 3-20, 5-10, 5-15, 5-20, 10-12, 10-15, 10-20, 12-20, or 15-20 amino acids in length.

In some embodiments, a peptide, e.g., a targeting peptide, of the present disclosure may be identified and/or designed by any sliding window algorithm known in the art.

In some embodiments, a peptide, e.g., a targeting peptide, and associated AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) may be identified from libraries of AAV capsid polypeptides, e.g., AAV capsid variants. In some embodiments, the peptide, e.g., targeting peptide, may be a 5-10 amino acid sequence, e.g., a 6-10 amino acid sequence, a 6-9 amino acid sequence, a 7-10 amino acid sequence, a 7-9 amino acid sequence, an 8-10 amino acid sequence, a 7 amino acid sequence, an 8 amino acid sequence, or a 9 amino acid sequence. In some embodiments, the peptide, e.g., targeting peptide, may be a 5 amino acid sequence (5-mer). In some embodiments, the peptide, e.g. targeting peptide, may be a 6 amino acid sequence (6-mer). In some embodiments, the peptide, e.g., targeting peptide, may be a 7 amino acid sequence (7-mer). In some embodiments, the peptide, e.g., targeting peptide, may be a 9 amino acid sequence (9-mer). In some embodiments, the peptides, e.g., targeting peptides, may also differ in their method of creation or design, with non-limiting examples including, random peptide selection, site saturation mutagenesis, and/or optimization of a particular region of the peptide (e.g., flanking regions or central core).

In some embodiments, a peptide library, e.g., a targeting peptide library, comprises targeting peptides of 7 amino acids (7-mer) in length randomly generated by PCR.

In some embodiments, a peptide, e.g., a targeting peptide, library comprises peptides, e.g., targeting peptides, with 3 mutated amino acids. In some embodiments, these 3 mutated amino acids are consecutive, or contiguous, amino acids. In another embodiment, these 3 mutated amino acids are not consecutive, or noncontiguous, or split, amino acids. In some embodiments, the peptide, e.g., targeting peptide, is a 5-mer. In some embodiments, the peptide, e.g., targeting peptide, is a 6-mer. In some embodiments, the parent peptide, e.g., targeting peptide, is a 7-mer. In another embodiment, the parent peptide is a 9-mer.

In some embodiments, a peptide, e.g. a targeting peptide, library comprises 7-mer peptides, e.g., targeting peptides, wherein the amino acids of the peptide, e.g., targeting peptide, and/or the flanking sequences are evolved through site saturation mutagenesis of 3 consecutive amino acids. In some embodiments, NNK (N=any base; K=G or T) codons are used to generate the site saturated mutation sequences.

AAV particles comprising capsid proteins with a peptide, e.g., a targeting peptide, insert (e.g., an AAV capsid variant) are generated and viral genomes encoding a reporter (e.g., GFP) encapsulated within. These AAV particles (or AAV capsid library) comprising AAV capsid variants are then administered to a transgenic rodent (e.g. mouse) by intravenous delivery to the tail vein. Administration of these capsid libraries to cre-expressing mice results in expression of the reporter payload in the target tissue, due to the expression of Cre.

In some embodiments, AAV capsid mRNA expression may be modulated, e.g., under the control of, or driven by, a cell-type specific promoter. Such capsids, which may comprise peptide, e.g., targeting peptide, inserts and viral genomes encoding a reporter encapsulated within, may be administered, e.g., by intravenous delivery to the tail vein, to a non-transgenic rodent (e.g. mouse), such as but not limited to a C57BL/6 mouse, a BALB/C mouse and a rat. Administration of such capsid libraries to a non-transgenic rodent may result in the expression of the reporter payload in the target tissue, due to the cell-type specific promoter.

In some embodiments, AAV capsid mRNA expression may be under the control of, or driven by, a cell-type specific promoter. Such capsids, which may comprise a peptides, e.g., targeting peptide inserts, and viral genomes encoding a reporter encapsulated within, may be administered, e.g., by intravenous delivery to the saphenous vein, to a non-human primate, such as but not limited to a cynomolgus macaque and a rhesus macaque. Administration of such capsid libraries to a non-human primate may result in the expression of the reporter payload in the target tissue, due to the cell-type specific promoter

In some embodiments, AAV particles comprising capsid proteins with peptide, e.g., targeting peptide, inserts may hereinafter also be referred to as peptide display capsid libraries.

AAV particles and/or viral genomes may be recovered from the target tissue for identification of peptides, e.g., targeting peptides, and associated AAV particles that are enriched, indicating enhanced transduction of target tissue. Standard methods in the art, such as, but not limited to next generation sequencing (NGS), viral genome quantification, biochemical assays, immunohistochemistry and/or imaging of target tissue samples may be used to determine enrichment.

A target tissue may be any cell, tissue or organ of a subject. As non-limiting examples, samples may be collected from brain, spinal cord, dorsal root ganglia and associated roots, liver, heart, gastrocnemius muscle, soleus muscle, pancreas, kidney, spleen, lung, adrenal glands, stomach, sciatic nerve, saphenous nerve, thyroid gland, eyes (with or without optic nerve), pituitary gland, skeletal muscle (rectus femoris), colon, duodenum, ileum, jejunum, skin of the leg, superior cervical ganglia, urinary bladder, ovaries, uterus, prostate gland, testes, and/or any sites identified as having a lesion, or being of interest.

In some embodiments a peptide, e.g., a targeting peptide, may comprise a sequence as set forth in Table 1. In some embodiments a peptide, e.g., a targeting peptide, may comprise a sequence as set forth in Table 2. In some embodiments, the peptide, e.g., targeting peptide, comprises an amino acid sequence of any one of peptide 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12, e.g., as described in Table 2. In some embodiments, the peptide, e.g., targeting peptide, is isolated, e.g., recombinant.

TABLE 1 Exemplary Peptide Sequences, e.g., Targeting Peptides Peptide Sequence SEQ ID NO: PLNGAVHLYA 1725 AQARDSPKGW 1726 LTNGAVRDRP 1727 VQAFTHDSRG 1728 AQAYSTDVRM 1729 AQAYSTDVRI 1730 AQAFTAAERM 1731 AQTHLQIGVA 1732 AQSNAVLSLA 1733 AQAYSTDERM 1734 AQAYSTDVRL 1735 AQATVSTLRM 1736 AQAYSTDERK 1737 AQAYSTDMRM 1738 VVNGAVLHVA 1739 AQAYSTDVTM 1740 AQAHLQIGVA 1741 FLDPAVSSKA 1742 AQAYVSTLRM 1743 AQAQTGPPLK 1744 EQASRLPTPG 1745 AQASVSTMRM 1746 TDYSAVRLGA 1747 TQAYSTDVRM 1748 AQALPSNERL 1749 AQAYSTDVRT 1750 AQSSLPEMVA 1751 AQAGEQSTRL 1752 AQASNDVGRA 1753 AQATFTASEY 1754 AKAHAGTIYS 1755 AQARTIDQCC 1756 AQEYNSNPKA 1757 AQVVDNSTHA 1758 AQATLSVPLK 1759 AQIVMNSLKA 1760 AQATMSQTMA 1761 AQALTQDERW 1762 AQAQLSTLRP 1763 AQVVMGISVA 1764 AQAYTTDVRM 1765 AQHIDSMRPP 1766 AQASTGTLRL 1767 AQHRALDYYA 1768 AQARESPRGL 1769 AQALLAGTRV 1770 TKIQAVPWNA 1771 AQASLSSTRP 1772 AQAMGSRSDQ 1773 AQAAQGTYRG 1774 SQENAVFSKA 1775 AQALSLSTRP 1776 AQAAAGTLRD 1777 AQASRLPTPG 1778 AQAGSLSERG 1779 AQSKGDGFTA 1780 GAGTAVTATA 1781 AQAQGSSSVG 1782 AQAYSTDARM 1783 ERAHAVTGLA 1784 AQAYGLPKGP 1785 AQAYSTEVRM 1786 AQAGVSTALH 1787 AQSYSTDVRM 1788 AQPLMSHTDA 1789 AQAAALASRP 1790 AQAAITSTIS 1791 AQPANDGLRA 1792 AQDYSTDVRM 1793 AQATLGYSTA 1794 AQATLGTIRV 1795 AQAGASDMVH 1796 AQAVSGTVRS 1797 GGTLAVVSLA 1798 AQAYSADVRM 1799 AQAFAMPKGL 1800 AQALVSTSRP 1801 AQASFQQAST 1802 AQAMTGNDRS 1803 AQASTQSPPG 1804 NARSAVESLA 1805 AQITVSHTTA 1806 AQALAGYDKA 1807 AQSTSHDTRA 1808 AQAIQDRTVV 1809 AQSKTTLTLA 1810 AQASMGTVRL 1811 AQHSDTLTRA 1812 AQKEMYTSVA 1813 AQASPSQPLL 1814 AQAYAGTIYS 1815 AQARSLEPVI 1816 TQAGVSTAVH 1817 AQNTLSLSLA 1818 AQAYVSSVKM 1819 AQAATSPRLG 1820 GYLTAVQPQA 1821 LNNLAVGMTA 1822 AQTVSVHVRA 1823 AQINGLVTTA 1824 AQAAITTTIS 1825 AQASTFVTTI 1826 AQALYDNVPL 1827 AQAAAGTWKG 1828 AQATTGTLRS 1829 GQYAADSSYA 1830 AQAGIATVRT 1831 AQALGHELRA 1832 AQAREAIPQG 1833 AQAMSGTLRM 1834 AQAVDRVRPP 1835 AQAPVNNDRG 1836 AQAQQVAGTM 1837 AQAEPRDTRA 1838 AQRLSEQGVA 1839 AQASEGIQLS 1840 AQRQGPDPLA 1841 AQVTLGSAKA 1842 AQAGASLGLA 1843 AQAFTQDERW 1844 AQASQTTVRS 1845 AQARVSSNGV 1846 AQGPLSGLRA 1847 AQAYGGQSLG 1848 AQANLGTVRQ 1849 AQARSDTRGL 1850 AQAGSDGPRL 1851 TDGAAVVMRA 1852 SGITAVPLHA 1853 AQAAAVGHLP 1854 AQRVEPKWIA 1855 AQAVASSPYA 1856 TQYGAVEGQD 1857 AQAKSHTLEG 1858 AQTHLQIVVA 1859 AQKNEHGMLA 1860 AQITVSHTRA 1861 AQARLAPKGL 1862 KTPGAVSTTA 1863 AQAFSGTIKS 1864 PLNGAVNLYA 1865 AQALTQDERC 1866 AQATAQVQRS 1867 AQTPALINLA 1868 AQASDRSPLL 1869 AQITVSHTMA 1870 AQATGTHLMG 1871 LDGGAVVVTA 1872 LTNGAVRDRA 1873 AQARGSDLRD 1874 AQATFGTQRI 1875 AQALPQTNRP 1876 AQARSNDPVL 1877 AQAYLAVQNG 1878 AQATQSTLRP 1879 AQALGGFGPQ 1880 LVGQAVGSRA 1881 AQSIANVVVA 1882 AQASPSVSRP 1883 AQTVVVSTTA 1884 VKEQAVSVMA 1885 AQQATGTFRA 1886 AQAQGSSSGG 1887 AQAHAVGPQG 1888 AQRLETKETA 1889 AQLAQGIGVA 1890 AQAVQSSFTI 1891 AQATYTASEY 1892 AQTSSQNLKA 1893 AQLVPSVAMA 1894 AQASPSAFAG 1895 AQALALVSAS 1896 AQASVGTTYT 1897 AQARVSSSGT 1898 NSMGAVLGAA 1899 AQHTDTLTRA 1900 AQPNLQPRGA 1901 AQADRHSSIV 1902 SPSVAVPSQA 1903 AQPGIVSTIA 1904 AQAQHSVGLP 1905 AQTNSGAILA 1906 AQDSYDVGRA 1907 EQAQGSSSVG 1908 AQAGVSTAVQ 1909 AQARDMLPLQ 1910 AQAMVGTLRG 1911 AQPNVVSTLA 1912 AQAGHVVTSD 1913 AQAYTTDERM 1914 TAVSAVQVMA 1915 AQAAAGTLRV 1916 VSNEAVHARA 1917 AQVLPQSLSA 1918 AQASVSTLRM 1919 AQAGLLLSVA 1920 AQANLVTGPL 1921 AQASQHSSMA 1922 GYSSAVSSVA 1923 AQVGVSPAVA 1924 DGTLAVPFKA 1925 AQAPPTSTAM 1926 AQATPANVRG 1927 AQAGSSNFLS 1928 AQLLAQDIRA 1929 AQPSSDGYRA 1930 AQALIGTLRT 1931 SVHGAVGILA 1932 AQPYVVSGAA 1933 AQWTHNITAA 1934 PTNAAVRTNA 1935 AHAYSTDVRM 1936 PLAAAVGMKA 1937 AQARDNSVML 1938 AQAQFPRNGG 1939 GALNAVNGVA 1940 AQASHQQGVP 1941 SYQSAVVPQA 1942 AQSIMGTIRA 1943 AQAYVSQAQG 1944 AQATGNQAHF 1945 AQVTVGTPIA 1946 AQAQTSTFRG 1947 SVHMAVTVSA 1948 AQAQSTLNLG 1949 AQDQTGPPLK 1950 HLAHAVSTAA 1951 AQALARDSSF 1952 AQLLSGTLKA 1953 AQASLLPTPG 1954 AQPMAGQSTA 1955 AQARSLEPDI 1956 AQFVTGNQDA 1957 AQATFKTSVP 1958 LNARAVEGPA 1959 AQALPNSGRP 1960 AQALNGSPEA 1961 AQATSLHPLP 1962 AQAVQPPLKN 1963 AQAMLSGTRI 1964 AQHVDLASKA 1965 AQASFATMRP 1966 AQAMPLNARS 1967 AQALVGQMRG 1968 VVNGAVLHLA 1969 AQAQTAPPLK 1970 AQGHGDLHRA 1971 AQAADRSPVH 1972 GALNAVTGVA 1973 AQAERMASLG 1974 AQAPPTTTRL 1975 AQAAVGQTLA 1976 AQSLGTGMHA 1977 AQSLGSPALA 1978 AQASVSVTRP 1979 AQATMSHTMA 1980 AQAVQSLTVG 1981 AQSQTGTYRA 1982 AQSLASVYAA 1983 STKLAVHEQA 1984 AQSHLFPTPA 1985 AQGTWSSSEA 1986 AQTPQGLTKA 1987 AQVSLGTQYA 1988 AQDSRLPTPG 1989 ASIQAVGVKA 1990 AQATMSEQRL 1991 TAQAAVQGMA 1992 AQAFNAAERM 1993 AQINFLSGVA 1994 PQHLAVSSEA 1995 AQALGNFPAV 1996 AQANASTVRV 1997 AQRIVDLTTA 1998 VRQVAVEGVA 1999 AQAPASSQKL 2000 AQQIDSMRPA 2001 AQAHGTSSLF 2002 AQVNSGIALA 2003 AQLHLAETRA 2004 AQALTHDERW 2005 NTVRAVIMEA 2006 AQAYVAGSRP 2007 AQDRAFVVSA 2008 AQAQEKQVFS 2009 AQACVSTAVH 2010 AQAFTHDSRG 2011 AQASHQGTVG 2012 AQAVLVTEQG 2013 AQAVVSTAVH 2014 AQATSRETKG 2015 YQQPAVSSRA 2016 AQANMGLSLS 2017 AQWTSSMSEA 2018 AQASISIMST 2019 AQASVAPLTC 2020 AQLVTVEKQA 2021 AQAATAGEKL 2022 AQALSHGPGG 2023 AQSNAHIEIA 2024 AQARSSSTGI 2025 AQAVGGDVTR 2026 AQAPRTVYQG 2027 AQALHNLGPA 2028 VRMGAVSDNA 2029 AQAFRTSQFT 2030 AQSSATMQRA 2031 AQTLAETYRA 2032 AQANGSIVLN 2033 AQARVADQLP 2034 AQAVKQGLYE 2035 AQAFSDGLKS 2036 AQVSVTPVKA 2037 VNGRAVSMMA 2038 SLVGAVAQMA 2039 AQARVSPVGL 2040 AQSNTTLTLA 2041 AQTSTEHLRA 2042 AQAGMGINLP 2043 AQANAHSLTL 2044 AQARFTTTEM 2045 AQLGYQEVKA 2046 AQAGQHASVF 2047 AQATGSNPRG 2048 AQAPVSPSIP 2049 AQTTLGVGTA 2050 AQASHLVSLA 2051 AQAPLTGLSV 2052 AQVSTSTLRA 2053 AQVQLGTLKA 2054 AQAHVSVSER 2055 AQLLLSGQTA 2056 TTSSAVLTPA 2057 AQFGADTVNA 2058 AQTFSSDNLA 2059 AQIHPANSRA 2060 AQSIGQFPVA 2061 AQVISPENLA 2062 AQALSAISAT 2063 AQAGVSASQM 2064 AQASTKTPLP 2065 AQAPPSTTAM 2066 AQAVSSDRMH 2067 AQAGSVTMRL 2068 AQAVLLGGAV 2069 AQAQRDMVTT 2070 AQAHHGSSLG 2071 VLSSAVGQRA 2072 AQAAGSVLLG 2073 AQAYPTDVRM 2074 AQWSRDAQSA 2075 AQQGLDMGRA 2076 AQAAQNHALV 2077 AQRSQIVEVA 2078 AQMSDVSGRA 2079 AKALTQDERW 2080 AQAVSSSTLT 2081 AQPSRLPTPG 2082 RTSTAVLDFA 2083 AQDLSSSIRA 2084 AQLLDGLTSA 2085 AQALIGLSKP 2086 AQASGTVRPP 2087 AQLLDTRYKA 2088 AQAPNTSFTA 2089 AQTHLQIGVD 2090 AQRLDTSQVA 2091 AQRTQDTLSA 2092 AQADIQSHAL 2093 PNMNAVGIKA 2094 AQAGVSTAVH 2095 AQASGKTFIG 2096 AQAGVQSTRL 2097 AQAQGAYPLV 2098 AQPYSTDVRM 2099 SSSVAVVTLA 2100 AQTYNGLNKA 2101 AQASVSKLRM 2102 AQRGSENEKA 2103 PITNAVLKTA 2104 TNSYAVSSPA 2105 PYQTAVAGAA 2106 GPALAVLGRA 2107 LSISAVPAKA 2108 AQTLGPLPHA 2109 AQAQQPLAHV 2110 AQTDGAWSKA 2111 AQALSGPPSI 2112 AQASSPSTRG 2113 AQASLASNRP 2114 AQNMALSTVA 2115 AQHSDTMTRA 2116 AQAMPRYPPL 2117 AQHIDSMSPA 2118 AQALPGTSRV 2119 AQAKSTQDVQ 2120 AQPLVSASKA 2121 AQAMSGTLRK 2122 AQTLILGAHA 2123 AQAGQARSQG 2124 AQRKDLSLVA 2125 AQALSAPMSL 2126 TNSLAVGMRA 2127 AQAPIGTVRP 2128 FIQAAVSSSA 2129 AQAEKPTHLL 2130 AQALSGDTTR 2131 AQAYIASGGT 2132 QLNQAVGTLA 2133 AQASGALDRP 2134 AQAQDTALRA 2135 AQAQAGMARG 2136 AQAQGSSAVG 2137 AQLLRDIGPA 2138 VDRGAVTQMA 2139 AQAVNVSKGS 2140 SVNTAVESLA 2141 AQARLPHTSS 2142 AQRNGSEVVA 2143 AQATDRVDRP 2144 AQASLSRERT 2145 AQAYSTHVRM 2146 AQHLSAGPTA 2147 LNGGAVSLRA 2148 AQAYGVSSVT 2149 AQFGSAVQLA 2150 AQAPPTSTRL 2151 AQVSTNWPKA 2152 AQTSTDLSRA 2153 AQAHSTDVRM 2154 AQATLTGHVS 2155 AQATTQGALT 2156 AQAAKASDRT 2157 AQGNEHGGRA 2158 AQALSTSLLL 2159 AQASLGSTYL 2160 AQAFSTVGAV 2161 AQLNGLVTTA 2162 AQASVRTLRM 2163 AQATMSRPWQ 2164 AQSSLPAMVA 2165 RETVAVGQYA 2166 AQAFGSEGRS 2167 SSGTAVEHRA 2168 FGTNAVIPRA 2169 AQAGQARSLG 2170 AQSFSSDNMA 2171 AQMNGLTGKA 2172 AQQNGKQHLA 2173 AQHIDSIRPA 2174 AQAADRLSTL 2175 AQFGLKDIRA 2176 AQAHQGGATL 2177 AQATYNSPKP 2178 AQAMSNMLRN 2179 VPISAVMSTA 2180 AQHSLGNTVA 2181 AQATSALSRL 2182 AQADRQTFPV 2183 AQAVNSMSIG 2184 AQALAIVSKN 2185 AQGQLQERFA 2186 AQFNGASAHA 2187 AQLGGQSPVA 2188 AQANGAYTDN 2189 AQNLSSSEPA 2190 AQSAIVLTTA 2191 ITRSAVPDVA 2192 GALKAVTGVA 2193 AQAVGQDYLR 2194 AQVTLNTPLA 2195 AQALTQDDRW 2196 AQAYSTNVRM 2197 AQAVAAPASL 2198 AQANPVSIMS 2199 AQASMQAVKD 2200 AQAVGGHSVA 2201 AQAVAASTRL 2202 GGTHAVSSFA 2203 AQAADSSGFR 2204 AQQSHVPQTA 2205 AQARVGNTNV 2206 AQTVSYSDLA 2207 AQAEHGLARS 2208 AQASNYPVAA 2209 VLLSAVGMAA 2210 AQALSGQNRG 2211 AQAWGQETRQ 2212 AQGYSTDVRM 2213 AQAGSVMSRE 2214 AQAGSLSARG 2215 AQATALAPKS 2216 AQAIRQNGSS 2217 AQLQDNLQLA 2218 AKAYSTDVRM 2219 AQSVDRTLLA 2220 AQAGQNSRLP 2221 AQTNLQPRGA 2222 PNTIAVGQRA 2223 AQAHATLSLS 2224 NHLRAVGSPA 2225 AQETDRNLRA 2226 AQARAETSGS 2227 AQHRELDSYA 2228 AQRHTSDVLA 2229 AQVGQTSSWA 2230 AQANSAALLM 2231 AQAIIERTAT 2232 AQSSRYEEKA 2233 QATGAVNPRA 2234 AQASYSVSVG 2235 AQQGHTVNNA 2236 AQASLPISTR 2237 AQHIDSMRPT 2238 AQAQDTENMR 2239 RTGAAVTGAA 2240 AQASSVRGMG 2241 AQPGIESTIA 2242 AQHVDLDSKA 2243 AQATTVPALG 2244 AQVNPTPQKA 2245 AQAGYISSAS 2246 AQTLILGDPA 2247 AQAASGLTMM 2248 AQALERPPSG 2249 TTYDAVHSKA 2250 AQAMLDSANG 2251 AQAMHQTDKF 2252 KSTVAVQSVA 2253 AQATAGTLIG 2254 GLKSAVTHVA 2255 AQAHSAYQGA 2256 AQSFSSDNLA 2257 AQLNMAASVA 2258 AQFSQAYNAA 2259 AQHLTAGLRA 2260 AQAHTVSPHL 2261 GILGAVLPRA 2262 AQHNSSSLLA 2263 AQAPQVAGTM 2264 AQHQDSRPMA 2265 AQRFQETGLA 2266 AQLTVSHTRA 2267 AQANLRTTMG 2268 AQAGLRDPRM 2269 AQHLLHGTAA 2270 RNQGAVASLA 2271 AQAGSSSVTW 2272 AQPHLQIGVA 2273 AQANSGAVLA 2274 AQLLGDAVKA 2275 SSGNAVSSLA 2276 AQVSVTMALA 2277 AQYHTRGFAA 2278 AQSTTKGTLA 2279 AQAQPPSARY 2280 AQAGLQGTAA 2281 AQPQGSSTFA 2282 TQYRAVEGQA 2283 AQAISTQLAG 2284 AQLGSNISHA 2285 AQTGLSGTVA 2286 AQRVDSSGRA 2287 AQAGLALNPN 2288 AQFYSDNSLA 2289 AQAVGAPQRL 2290 AQASYDDGRA 2291 SSFAAVATAA 2292 AQSTLSMPLA 2293 AQASLHAPRP 2294 HAVAAVSYPA 2295 AQTSPVMVQA 2296 AQADITSTIS 2297 AQAAGVAMLY 2298 AQASVSTLRK 2299 MDLKAVSSRA 2300 AQASLSTLRM 2301 AQPRSPLPMA 2302 GQYADVSSYA 2303 LVGGAVVVPA 2304 AQAQSARPLA 2305 AQSLHPSTTA 2306 AQFQTDLSRA 2307 RTELAVGLSA 2308 AQVVDNSPLA 2309 AQAVSSDSMH 2310 AQASPALHTL 2311 GQYAAVASYA 2312 AQLWQSRVDA 2313 GTFSAVQSTA 2314 AQAILSTIEV 2315 AQNVVSTLRA 2316 AQAMLAVSPG 2317 AQATDSLVAR 2318 AQASPQSSHG 2319 AQATPVHDTL 2320 LRSSAVGTAA 2321 MGRGAVLDTA 2322 AQSHLIPTPA 2323 AQAVLKAPIN 2324 AQKIAPAFLA 2325 AGNVAVLPHA 2326 AQSLGTGLHD 2327 AQAHAMSSRP 2328 AQQGKFDMRA 2329 AQALSGDGTR 2330 AQTHLQIAVA 2331 AQRTQGSSWA 2332 AIGSAVDLRA 2333 AQAQLASGTL 2334 AQALVSAGAL 2335 AQATESVPLK 2336 AQVYNSNPKA 2337 AQRTTYPSSA 2338 AQAMFQQAST 2339 AQPDALVIRA 2340 AQARDISMRG 2341 AQMSFGSTLA 2342 RLLSAVDQQA 2343 AQTTRSIENA 2344 AQVSDFSSRA 2345 AQAHSRVNTE 2346 AQAYSTDLRM 2347 AQALNGSAYS 2348 TQYGAVEAQA 2349 AQAHAGTIYS 2350 AQDPHSMRPA 2351 AQASANIHSS 2352 AQASLQAVSM 2353 AQSSHPAMVA 2354 AQANLQPRGA 2355 AQAVGSSPRG 2356 AQTSAPSALA 2357 AQAVQLQNRG 2358 AQAQGSGMVS 2359 AQAYPSSKSG 2360 AQAVSDYGRG 2361 AQMSLGATRA 2362 AQAFLNSASA 2363 AQALPSNARL 2364 AQANVSVRRE 2365 AQAGASVMVH 2366 AQSLAKDQSA 2367 AQILSALSSA 2368 AQSVHLSLAA 2369 AQALSASSFL 2370 AQTSQLNQTA 2371 AQSNLFPTPA 2372 AQAHGRSFDT 2373 AQLGSNTILA 2374 AQASMNSAKA 2375 AQRQAVEQSA 2376 AQASTGTLRH 2377 AQGPTYPNVA 2378 AQAGPTTSKA 2379 AQATTYRGMA 2380 AQVTNRGMPA 2381 AQAISGQAAW 2382 AQAFRGEDKG 2383 AQQSMPRFVA 2384 AQAGVKSTRL 2385 AQATGSILLA 2386 AQASGHSSFS 2387 AQTANDGLRA 2388 AQASQLALLA 2389 AQLVDRVPRA 2390 AQHSNGYVHA 2391 AQAAPSSSDS 2392 AQAMQRSSSA 2393 AQAASGRPTC 2394 AQPRPGDSRA 2395 AQRDRANGIA 2396 AQVLAISLSA 2397 AQAGMRDPRM 2398 AQASSNSSRA 2399 MHRDAVSGVA 2400 AQAEMKNMPP 2401 AQSGSLLASA 2402 AQAFASQSRG 2403 AQALHLPTLQ 2404 AQAKTGGMNT 2405 ISLNAVSGKA 2406 AQGVHGHYVA 2407 AQAYSKDVRM 2408 VPSIAVSSHA 2409 AQSSRHDDLA 2410 AQANGSGSRG 2411 AQVGIADRRA 2412 AQARGMESML 2413 AQAGVSTAGH 2414 AQVSTRNLIA 2415 AQAVPRLTAG 2416 AQRHMELQEA 2417 SQSRAVVWEA 2418 QSHTAVSSLA 2419 AKASVSTLRM 2420 AQASGSSQWA 2421 AQPNAQYMKA 2422 AQAMGTGSSL 2423 AQAFSTSQLT 2424 AQAKDQSQRL 2425 AQVGGNGSRA 2426 AQANGASRAV 2427 QVNKAVLDFA 2428 AQETLSSTRA 2429 GVYGAVHSSA 2430 AQTITIENVA 2431 AQALMKIADG 2432 AQANVSLQAA 2433 AQSTTSHLRA 2434 AQLSNLVSVA 2435 AQANSTPTRQ 2436 AQQRGDRAAA 2437 AQARLGQSVG 2438 AQQLTYGSSA 2439 AQPAEKQYSA 2440 AQAMPRSRGD 2441 AQGLSGRALA 2442 AQARVTAVDA 2443 AQVGVSTAVA 2444 AQTGVTSAQA 2445 AQALVTSSEK 2446 AQASPHSSMA 2447 AQALTQDEMW 2448 AQAFSTQQRL 2449 AQAGSQVTQA 2450 AQQSTLALKA 2451 AQALNGSHAA 2452 AQATEGHLRS 2453 AQPMANMLMA 2454 PSTSAVSQKA 2455 AQAPPSSTEM 2456 AQRERVDLAA 2457 AQASVTLPRT 2458 AQAYPSSSKA 2459 AQAHSGSAIP 2460 AQSPSQSLKA 2461 AQATPPATSP 2462 CLGAAVNQCA 2463 VLGQAVRDKA 2464 AQAQKANNVG 2465 AQTLLPVNGA 2466 AQAHGTIQRG 2467 TVYTAVGVSA 2468 LGRGAVLDMA 2469 AQANVRSDQM 2470 AQARDSQKGW 2471 AQTPGSRSAA 2472 AQALPSNARQ 2473 AQASATSVVH 2474 AQINLGTMRA 2475 AQVYNNTSAA 2476 AQASANLTRG 2477 AQLRTDYTRA 2478 AQAYSTDVKM 2479 AQTSQLYQPA 2480 AQALTQEERW 2481 LPNGAVRDRA 2482 VTGSAVAGIA 2483 AQAFSTDVRM 2484 AQAHGPTSGV 2485 AQAGVGLPIA 2486 AQVNSGQARA 2487 AQAQTGPPMK 2488 AQARLAPVAC 2489 LSIGAVASMA 2490 AQAQDLGVMR 2491 PTGLAVTSPA 2492 AQSASTSWSA 2493 AQNGSNVRNA 2494 AQASISSSAT 2495 AQNSHAHLAA 2496 AQAVGVKQFF 2497 AQAHLSPTHA 2498 AQPAYGSSYA 2499 AQAHQARSGS 2500 AQAHTSPTQR 2501 AQAATPSSSR 2502 AQAHNSYPKV 2503 AQSSLGSSLA 2504 AQALSRSNVG 2505 AQASLSSLSG 2506 AQHGSSEFTA 2507 AQSALVAGVA 2508 AQASSSSLRP 2509 AQTARDTGFA 2510 KSVQAVRDPA 2511 AQAGSHSSVS 2512 VRAHAVTGLA 2513 ASHTAVGEFA 2514 AQIRSEWRDA 2515 AQQLARVSGA 2516 AQAAITSTNS 2517 AQARDAVQLP 2518 AQAKELVSTS 2519 AQGIAETLSA 2520 AQLGSGFSTA 2521 AQNAKELERA 2522 AQTHLQNGVA 2523 SGNLAVGTPA 2524 AQPSPGTSVA 2525 AQSSAAAGRA 2526 AQAGISAAIM 2527 AQALGYHQTG 2528 NAGQAVAARA 2529 AQPFGGSGYA 2530 AQAGSPSRLC 2531 AQARTIGTIA 2532 AQVVSVSSRA 2533 AQAGQARSMG 2534 AQATRGVTAG 2535 AQQSNGYGRA 2536 AQASLAPLKS 2537 AQPGANHNGA 2538 LGRGAVPDTA 2539 AQHFQTASLA 2540 AQAPAGHHTR 2541 AQPSVQNSMA 2542 AQAKLSGHVS 2543 AQFGTSSPSA 2544 AQASHISSVR 2545 AQALSRNGIG 2546 AQASAQVQRS 2547 AQGGPHLQAA 2548 AQAQSDSAFR 2549 AQTYSTDVRM 2550 LARGAVLDTA 2551 AQASPHTLRS 2552 AQHSDTQTRA 2553 AQATPSPSAS 2554 AQNQVTYSKA 2555 AQHTSVVYGA 2556 AQAQVSQMSH 2557 SFLRAVKNDA 2558 AQAYSTDVGM 2559 AKTPALINLA 2560 VSTAAVSSAA 2561 AQAPITSTIS 2562 AQTNLQTRGA 2563 AQATRLPTPG 2564 PQHLAVSSAA 2565 AQASPHPSRP 2566 AQAQPAGQRG 2567 AQPQRQGVQA 2568 AQHVAGSSNA 2569 AQVPIQMGVA 2570 AQATVSVPLK 2571 AQISVSHTRA 2572 SLVGPVAQMA 2573 AQPRLNLTEA 2574 AQASQEYSRL 2575 AQKSLAFDSA 2576 AQALGHSHHC 2577 AQAAQTGRPI 2578 AQASGTSVRQ 2579 AQAMGTASYC 2580 AQISHNHPQA 2581 AQAYSTYVRM 2582 AQVGKLDIRA 2583 AQLKQGGINA 2584 AQASAHFREP 2585 AQALDTVLSA 2586 GAGTAVGNIA 2587 AQANGSATYA 2588 AQTQLAQQKA 2589 AQYVTTVSPA 2590 SQFSAVTVTA 2591 AQAASDSFRY 2592 AQASPASVTR 2593 AQARDSGMFL 2594 AQSKTTLTLS 2595 AQLVQESLSA 2596 AQAALKSLAG 2597 AQAVPNQGQK 2598 AQALSRSSLG 2599 AQAGSVMSRV 2600 AQMATVTPMA 2601 AQARTASGID 2602 SHSSAVSHPA 2603 AQADRMRTTA 2604 AQNAQNRALA 2605 AQAASNAYSS 2606 AQATFQQAST 2607 AQVYTISTPA 2608 AQTVIAVGVA 2609 LARGAVPPTA 2610 AQMLQTSVLA 2611 AQARQVSPLL 2612 AQAGQMSNAR 2613 AQTPALIKLA 2614 AQAYTTDVRK 2615 VVKGAVLHVA 2616 AQDTVSVPLK 2617 AQKGAPSLQA 2618 AQASYDVGRA 2619 AQGPLSGMRA 2620 AQALGTSVPA 2621 AQVNKGASTA 2622 AQLTRTSPVA 2623 AQADAALRFS 2624 AQVQLVVSPA 2625 AQAYSSDVRM 2626 AQARSGLSLP 2627 AQNGHKFTAA 2628 AQGLSSATKA 2629 AQGTWSTVKA 2630 AQASGVGGRI 2631 AQTSYPAQKA 2632 AQNAVPTHSA 2633 AQSYPEITRA 2634 AQTGLSTSSA 2635 AQYDTHNFAA 2636 AQAVLSSVIQ 2637 AQDSAVALMA 2638 AQATGKGALP 2639 AQNSRSGHDA 2640 AQAFQKEPSV 2641 AQAGSTSGKM 2642 AQRDQAHSQA 2643 AQAASALSGR 2644 AQARHSSLLP 2645 AQGPGTSYLA 2646 FLAPAVSSKA 2647 AQAGPQCSSC 2648 AQALTQHERW 2649 AQAIRSSERV 2650 AQAVHSSSVY 2651 AQSSRTALAA 2652 AQITFSHTRA 2653 AQALTLSGGL 2654 AQAGKTLSVL 2655 AQASRSNLDN 2656 AQGSLSTHTA 2657 AQQSVAYNVA 2658 AQHTLRLSSA 2659 AQAGGTPNKL 2660 AQAFQSLTLA 2661 AQAVALSHQE 2662 AQMLASGIPA 2663 AQNRALDSYA 2664 AQASGSTTRN 2665 AQARGDGYVA 2666 DARVAVLDFA 2667 AQAVASQVSR 2668 AQARGPSPAT 2669 AQHRALDSYD 2670 AQLIDSTSRA 2671 AQAQTLSRGS 2672 AQFRSAITSA 2673 AQANMTKQSL 2674 AQNAGSTSRA 2675 VLGSAVTGRA 2676 AQPMLQSSSA 2677 AQLGTPSLSA 2678 AQATAHTGVP 2679 AQAVGRDNRL 2680 AQATSASVWA 2681 AQAGSEASLR 2682 AQANQNRTAF 2683 AQASAQVKRS 2684 AQATSGVHHP 2685 AQTHMQIGVA 2686 AQSHIFPTPA 2687 AQLFHTGSPA 2688 LASRAVVGTA 2689 AQALLRVGVG 2690 AQITLPSGTA 2691 AQAEKSLGRQ 2692 AQTSNTTTRA 2693 AQAHTQASYM 2694 AQERGASSSA 2695 AQATPSSTAM 2696 AQSTVNRTYA 2697 AQAGHGPSTR 2698 AQLSLVPLQA 2699 AQLHSPIPSA 2700 AQSLARDGLA 2701 AQAPPSSPAM 2702 TQYGAVERQA 2703 AQAGQARSLA 2704 AQPVGRVPPA 2705 AQAREQRGPV 2706 AQKTSLLWEA 2707 AQGSGKNLIA 2708 AQASEGHQLS 2709 AQALHAGHHP 2710 AQSKRDDPSA 2711 AQTSRELRMA 2712 AQALPASGAR 2713 AQSNALLSLA 2714 AQASPVVGVS 2715 AQARGDSYMA 2716 AQAGASSLTV 2717 AQALRPVNGT 2718 AQVRSGPTLA 2719 AQFPPLSRSA 2720 AQVARGTVQA 2721 AQTSTQSPPG 2722 AQARDGMNVR 2723 AQAVSRNVVV 2724 AQHTATRSVA 2725 AQAVREDGHA 2726 TNSSAVAASA 2727 AQATFQLAST 2728 AQAHHQQTSL 2729 AQGQHAHMMA 2730 AQATSSLHVL 2731 AQAPNSGLAM 2732 SASRAVLDFA 2733 AQARGEQRFV 2734 AQTHLQIRVA 2735 AQAPPSSKAM 2736 AQIVSKAMPA 2737 AQASVRNNPS 2738 AQAESRVAAL 2739 LTNGAVRDRT 2740 AQGRLAGSLA 2741 AQAGQDSARR 2742 AQAASRLGAV 2743 AQALARGMAS 2744 AQASRGLSMG 2745 AQAQASSYGS 2746 AKASRLPTPG 2747 AQSLSRASTA 2748 AQASTFVQTI 2749 AQASSKVVAA 2750 AQAYRNGEAA 2751 AQAYSTGVRM 2752 AQAVSSRSMG 2753 AQARGGLATP 2754 AQAGHSGVRA 2755 AQPSYHGGAA 2756 AQRVNQVSTA 2757 AQAAFQQAST 2758 AQAVPGSPRA 2759 AQLSLSPLAA 2760 AQANMTVRVS 2761 AQATRSSGDP 2762 AQVASNATRA 2763 AQTNQQPRGA 2764 AQRLQNDHLA 2765 AQAPVQLGRP 2766 AQRQGPDTLA 2767 AQHTLSNHMA 2768 AQLSGMVNRA 2769 AQDRQVSSRA 2770 AQRQLSTSLA 2771 AQQRPTVSFA 2772 AQAKPHSQLD 2773 AQAGRVNHPP 2774 AQAINSQSMR 2775 AQYSTAVMSA 2776 SQARAVERSA 2777 AQAYKSSSVG 2778 AQASTPGLYP 2779 AQSRTSMLAA 2780 AQLFSSNMPA 2781 AQAYCTDVRM 2782 AQTMSRGFVA 2783 AQALNGYPAA 2784 AQAQTGHPLK 2785 AQASSNSQYR 2786 AQAAIKSTIS 2787 AQSTLNLRPA 2788 AQATLSPGSG 2789 AQANGSGTGR 2790 STSLAVAGRA 2791 AQASNLSAYR 2792 AQASRQVLVA 2793 NEVRAVFFEA 2794 AKAQGSSSVG 2795 ARGSAVQSQA 2796 AVRVAVSSSA 2797 AQAFSTSQFK 2798 AQGTSSQRTA 2799 AQATMSQTIA 2800 AQSANRSTLA 2801 AQRDLAHSKA 2802 AQASKVGLYA 2803 AQAYYTDVRM 2804 AQAGLRDPRA 2805 AQAFSQATGA 2806 AQVAGMSVRA 2807 AQAGQSSFTI 2808 AQKEMRSQGA 2809 AQNYSSGVRA 2810 AQITVSYTRA 2811 AQAQQPRSSI 2812 WTSGAVPGKA 2813 AQFKPSQVIA 2814 RQGQAVGSSA 2815 AQSISPHYAA 2816 AQARSLNEYK 2817 AQAASSRLMA 2818 AQAYSTDGRM 2819 AQASVPRVMG 2820 AQGQMPRYPA 2821 AQASSGMKPC 2822 AQPLRSSLSA 2823 AQNSASQSQA 2824 AQGHLSGLRA 2825 AQRAQSGVAA 2826 AQANPRLQDK 2827 AQAPRTATLG 2828 AQRTASLSQA 2829 AQGNPGLLRA 2830 MSSHAVGNRA 2831 AQLAPKASPA 2832 AQTTQGRERA 2833 AQASGKSTSS 2834 AQAPHQHSMK 2835 GKLGAVVAQA 2836 AQAFQKVQSV 2837 AQTVAQSRVA 2838 AQAPPSQTSR 2839 AQAGVGYSSK 2840 AQMGSPSSKA 2841 QSSRAVLDFA 2842 AQARQTLDFG 2843 AQSIRSASQA 2844 AQFLSPKVTA 2845 AQAAGSGHTR 2846 VSSSAVIVGT 2847 AQAYSTDVMM 2848 AQALIGTNPR 2849 AQVMPSPSRA 2850 AQITVSHTRS 2851 AQGPHSGLRA 2852 AQANGRVQVT 2853 AQAMPRTASV 2854 AQVSTKWPKA 2855 AQAMPRYPGE 2856 AQAATTPMSR 2857 AQARGDHFSI 2858 AQAKTNTSHA 2859 AQNTPRINQA 2860 AQATVALPRG 2861 AQGSKVGLNA 2862 AQAHQNLRGA 2863 AQIVSTLQHA 2864 AQTGSSLSRA 2865 AQVRTALASA 2866 AQAYSTVVRM 2867 AQQMPRIVPA 2868 AQALTQYERW 2869 AQASRQPPAQ 2870 AQAYYTDERM 2871 TQITVSHTRA 2872 AQASSGTRSA 2873 AQAKVSVPLK 2874 TQYGAVEGQA 2875 AQANSTSSTR 2876 AAGTAVTATA 2877 AQAAPPHRLS 2878 AQMMPRMPPA 2879 AQTHLQIWVA 2880 AQTNLKPRGA 2881 AQTNLQIGVA 2882 ILAPAVSSKA 2883 AQAHKVLDFG 2884 AQAYSTDVSM 2885 AQAYSTDVWM 2886 AQAGMPRGPS 2887 AQHLPALKMA 2888 AQAIGLNESA 2889 AQTQLQIGVA 2890 AQAYSPDVRM 2891 AQANSTDERM 2892 HATVAVQGAA 2893 AQACSTDVRM 2894 AQIKSLTSVA 2895 EQAYSTDVRM 2896 AQANSTDVRM 2897 AQAMPRTPGV 2898 GEGTAVTATA 2899 PTNAAVRTKA 2900 AQTHLQIGEA 2901 AQADSTDVRM 2902 VTFAAVTDKA 2903 AQAYYTDVRK 2904 EQTHLQIGVA 2905 AQAYSTDVRV 2906 AQQFQTQNAA 2907 AQITVSHTSA 2908 AQLGRMQYAA 2909 AVRVADSSSA 2910 AQIQTDLSRA 2911 AQAYFTDVRM 2912 AQAYSTDVRK 2913 TQAHAGTIYS 2914 AQARSLEPVN 2915 AQAYVSQGMS 2916 EQALTQDERW 2917 AQAGLSTAVH 2918 AQAGLSDPRM 2919 PTNAADRTNA 2920 AQAPQVEGTM 2921 AQAFTPAERM 2922 AQGPLYGLRA 2923 AQTNLQQRGA 2924 AQPRFNLTQA 2925 AQAYSMDVRM 2926 AQLGGQSQVA 2927 AQGSKDGLYA 2928 ALAYSTDVRM 2929 AKSKTTLTLA 2930 EQAFTAAERM 2931 AQDSVSTLRM 2932 AQVYSTDVRM 2933 AQGNMLVKDA 2934 AQARSLEQVI 2935 AQAFSTSQFT 2936 LTYGAVRDRA 2937 AQSLGTGLHA 2938 AQAAITSTIT 2939 GALNADTGVA 2940 AQRLKNDHLA 2941 VQAYSTDVRM 2942 SVHGAVGIMA 2943 AQTSQLYQNA 2944 AQARSMEPVI 2945 SQAYSTDVRM 2946 AQRLQNDNLA 2947 AQEVDNSTLA 2948 VSTEAVSSAA 2949 AQASPLPTPG 2950 AQARRLEPVI 2951 AQAQTGTPLK 2952 AQSKTTFTLA 2953 AQTTFQQAST 2954 AQLSTSGLLA 2955 AQASGALDRT 2956 AQTVSVHDRA 2957 AQSKTTLTLP 2958 AQATFQQASS 2959 AQAKGSRSDQ 2960 AQAANTSTIS 2961 SVNTAVASLA 2962 AQAPPSSTAM 2963 LTNGSVRDRA 2964 AQRQGPDPMA 2965 AQARSLEPLI 2966 AQAGRMSQSG 2967 AQTLMSHTDA 2968 AQAPGMGAPL 2969 GAGPAVTATA 2970 AQAQPGPPLK 2971 AQLIDSMRPA 2972 AQAQTGPPQK 2973 AQARVAVKLP 2974 AQARTNNSSG 2975 AQARSLAPVI 2976 AKAFTAAERM 2977 AQNVDLASKA 2978 AQARTIDKCC 2979 AQAPPSSTAK 2980 AQSNSVLSLA 2981 AQTLILGATA 2982 AKATMSQTMA 2983 AQAQTGPPLQ 2984 AQPGIVSTID 2985 AQHRALDSNA 2986 QTNAAVRTNA 2987 AQAPQVAGTK 2988 AQHGFTDSSA 2989 EQTPALINLA 2990 SVNTDVASLA 2991 AQAHPSSTAM 2992 AQYISDTYRA 2993 AQHIDSMRPA 2994 AQTSGQDERA 2995 AQAGDAAVRY 2996 AQAGRYESGN 2997 AQSMQSRSEA 2998 AQRLQNDHMA 2999 AQPVSVHVRA 3000 AQASEGLKLS 3001 AQLYVSSSVA 3002 AQATGVSQQM 3003 AQAPSKELFM 3004 AQAAITRTIS 3005 GLKSAVTHAA 3006 AQALGVTQSP 3007 AQAPSHLVPV 3008 AQARVAVQLQ 3009 AQAGSRLALE 3010 AQYGAVEGQA 3011 AQHSNKQVMA 3012 AQATFQQAGT 3013 AQAPTSSTAM 3014 AQHQDSRTLA 3015 AQAQRPMTSV 3016 AQAHARQMTV 3017 AKAFSTSQFT 3018 AQATPRQASH 3019 AQAAGSLRLG 3020 AQAQSRQLAM 3021 AQAMPGPMSR 3022 AQAPPRSTAM 3023 AQAAITSTIR 3024 AQALRGAALP 3025 AQASVQGISR 3026 AQAMMSRQSV 3027 AQARQSPLSG 3028 AQAGNVASTR 3029 AGRVAVNLVA 3030 AHSYAVTSTA 3031 AISGAVYSPA 3032 AKAGQSSFTI 3033 ALKHAVDVLA 3034 ALNKPRINQA 3035 ALNTPRINKA 3036 AQAAPVQSGV 3037 AQAAQSSFTI 3038 AQAARHSTTG 3039 AQAARIAQAS 3040 AQAARSTVYT 3041 AQAARTAPGL 3042 AQAARTSAVS 3043 AQAARYVTGV 3044 AQAASFLTAD 3045 AQAASGGSFT 3046 AQAASMRDHT 3047 AQAASVRQAR 3048 AQAAVDTTYR 3049 AQADCKFVVV 3050 AQADPSYRAN 3051 AQADRMVLRS 3052 AQADRPMVHR 3053 AQADTGASMQ 3054 AQAEGNGHWR 3055 AQAELQSGIR 3056 AQAEMRSSSR 3057 AQAESFLTAV 3058 AQAETRSGLS 3059 AQAFITSQFT 3060 AQAFLPMGGA 3061 AQAFRGASAL 3062 AQAFSSTHSR 3063 AQAFSTTQFT 3064 AQAGCSKELR 3065 AQAGDIRLSL 3066 AQAGDSRQGS 3067 AQAGGLHGVA 3068 AQAGHRTPGP 3069 AQAGHVVTYV 3070 AQAGLRDPSM 3071 AQAGLSQVIQ 3072 AQAGNSYSQR 3073 AQAGRGTIMA 3074 AQAGRLSKSG 3075 AQAGRLSQSG 3076 AQAGRLYQSG 3077 AQAGRPAFPW 3078 AQAGRQDLFS 3079 AQAGRSDSSL 3080 AQAGRTMNWS 3081 AQAGRVEVHL 3082 AQAGSSVSRA 3083 AQAGSTGGSA 3084 AQAGTRSMLA 3085 AQAGTSTPRW 3086 AQAGVNLRAL 3087 AQAHDRLPSG 3088 AQAHNAGVPD 3089 AQAHNSDRAR 3090 AQAHRGEVGQ 3091 AQAHRHISGP 3092 AQAHSRSMSQ 3093 AQAHSVNTAS 3094 AQAHTSTVLC 3095 AQAIALHRPS 3096 AQAICLSPDR 3097 AQAIEGRYPR 3098 AQAIPSPYSV 3099 AQAIRGAVQA 3100 AQAKSSPLSV 3101 AQAKSSSPTL 3102 AQAKTASSPF 3103 AQALATLPAM 3104 AQALEHTSPR 3105 AQALGGFRSE 3106 AQALGHQGQL 3107 AQALIPMVRG 3108 AQALQASRPP 3109 AQALQPFSFH 3110 AQALQQPQSR 3111 AQALRASAPR 3112 AQALVGSRGQ 3113 AQALYQHHSI 3114 AQALYRQSET 3115 AQAMASSLLA 3116 AQAMGPVGKC 3117 AQAMGRESYN 3118 AQAMGSNERY 3119 AQAMGTADRA 3120 AQAMITTVHS 3121 AQAMPRLRDA 3122 AQAMRSLSAA 3123 AQAMSRLGTA 3124 AQAMSRTVMP 3125 AQANCLSPDR 3126 AQANFPFGGP 3127 AQANGRDVRT 3128 AQANKDEGMG 3129 AQANLQQSSG 3130 AQANLQRSSA 3131 AQANQVRSPL 3132 AQANSSLSSR 3133 AQAPGQGSQT 3134 AQAPHRGLYT 3135 AQAPHSSTAM 3136 AQAPKVLDWG 3137 AQAPMSRLAQ 3138 AQAPMTSGVQ 3139 AQAPNNGVQK 3140 AQAPQTRPGR 3141 AQAPREHPSR 3142 AQAPRTPDLR 3143 AQAPSSDRVH 3144 AQAPSSQEAR 3145 AQAPSSSYMR 3146 AQAPTSASRA 3147 AQAPTSCLAT 3148 AQAPYTPSSF 3149 AQAQGAYREM 3150 AQAQKSPQFR 3151 AQAQLGTSSP 3152 AQAQPNYASV 3153 AQAQRGLHAV 3154 AQAQSQSWSS 3155 AQAQTRPPLK 3156 AQAQTSRAMD 3157 AQAQVSQMSL 3158 AQARAAHMAQ 3159 AQARAAVPSK 3160 AQARAQGHSY 3161 AQARASYNSM 3162 AQARATLGST 3163 AQARDASGVA 3164 AQARDRMMIN 3165 AQARDRMMYN 3166 AQAREQNVSS 3167 AQARFPSVYA 3168 AQARFTTNEL 3169 AQARGDMFAW 3170 AQARGDSGVS 3171 AQARGLAEVE 3172 AQARGNNLYT 3173 AQARGSPMSR 3174 AQARGTQTAE 3175 AQARGTTTAE 3176 AQARIDHGRC 3177 AQARITTDMS 3178 AQARLPMLVG 3179 AQARMPVVFG 3180 AQARNGLMTM 3181 AQARPPLGRL 3182 AQARPTGFTL 3183 AQARQSSFTI 3184 AQARSGVASL 3185 AQARSLEPGI 3186 AQARSPVVEK 3187 AQARSSDRGS 3188 AQARTIDQSC 3189 AQARTPLGYQ 3190 AQARTTSFVA 3191 AQARVDVQLP 3192 AQARVQIEKH 3193 AQASAKPLIE 3194 AQASARMPSS 3195 AQASAVRMAR 3196 AQASDPPRSS 3197 AQASDRVSRG 3198 AQASGIAAGA 3199 AQASGYTSVS 3200 AQASHSTLGT 3201 AQASLKILSL 3202 AQASLQILSL 3203 AQASLQILTL 3204 AQASLQIMSL 3205 AQASMQILSL 3206 AQASNRLDRP 3207 AQASQAQPKS 3208 AQASRESPTR 3209 AQASRIHSCC 3210 AQASRNLSAG 3211 AQASRTSPPL 3212 AQASSKSACQ 3213 AQASSRVSSF 3214 AQASSTSISK 3215 AQASVSGRAL 3216 AQASVSRSLL 3217 AQASYKPSLM 3218 AQASYQPSLM 3219 AQASYQTSLM 3220 AQATALLHQM 3221 AQATCQQAST 3222 AQATFLQAST 3223 AQATFQQTST 3224 AQATFSKGIV 3225 AQATGSDSRR 3226 AQATGSSGLS 3227 AQATLGKPSV 3228 AQATLPMLTR 3229 AQATRLSAAL 3230 AQATRQAHPS 3231 AQATRSDTIP 3232 AQATRSNGGV 3233 AQATSGRPVC 3234 AQATSGSSSQ 3235 AQATTIQSAA 3236 AQATTRATLS 3237 AQATTSNTRA 3238 AQATVPLFWL 3239 AQAVAMSPHA 3240 AQAVEVGATR 3241 AQAVGIQKLL 3242 AQAVITSRAP 3243 AQAVLPIALG 3244 AQAVRASTSL 3245 AQAVRLPAFV 3246 AQAVRSGVIK 3247 AQAVSGRLST 3248 AQAVVGRSVP 3249 AQAVVTRSPP 3250 AQAWPLASQS 3251 AQAWQSNMER 3252 AQAYGPLNTL 3253 AQAYHPSMKV 3254 AQAYKAPYGA 3255 AQAYNQTSRL 3256 AQAYNTHSPT 3257 AQAYQKVPSV 3258 AQAYTSQLNS 3259 AQCGSLCRTA 3260 AQDFSTSQFT 3261 AQDFTAAERM 3262 AQDHVQRSSA 3263 AQDKKSWPPA 3264 AQDLVTPSRA 3265 AQDRCTFVEA 3266 AQDRNNTRVA 3267 AQDRQLLWEA 3268 AQDTKTIGWA 3269 AQELRGSKTA 3270 AQESGMPMDA 3271 AQFESDIYSA 3272 AQFMSVSRSP 3273 AQFMSVYRSP 3274 AQFSPRVTGA 3275 AQFSREPTRA 3276 AQFTSFPSSA 3277 AQGFAPTKLA 3278 AQGHVGLPYA 3279 AQGLYDAKVA 3280 AQGMFGAQNA 3281 AQGMFNSSSA 3282 AQGMTNHASA 3283 AQGNGSRITA 3284 AQGPGSRISA 3285 AQGPSNTRAA 3286 AQGQEFMNFA 3287 AQGRGANYMA 3288 AQGRGDVVSA 3289 AQGRQSSLAA 3290 AQGRQTDRVA 3291 AQGSGPRYMA 3292 AQGSKVGLYD 3293 AQGSKVGMYA 3294 AQGSNVRSYA 3295 AQGSVRSPLA 3296 AQGTMGHHLA 3297 AQGVRTMVTA 3298 AQHIASMRPA 3299 AQHIDSMRLA 3300 AQHQASLGTA 3301 AQIDRAYPLA 3302 AQIDRAYTLA 3303 AQIDREYPLA 3304 AQILHAKLAA 3305 AQILRVANSA 3306 AQIPGRPWDA 3307 AQIPSGVANA 3308 AQIRIPYSSA 3309 AQISGMTSRA 3310 AQISRPLGSA 3311 AQKANVTGRA 3312 AQKELVAPHA 3313 AQKFAFVSAA 3314 AQKIAPHWSA 3315 AQKSDRHPQA 3316 AQKSWPQTVA 3317 AQKTTFPSSA 3318 AQLALKKTAA 3319 AQLDLTIGRA 3320 AQLDTMQGKA 3321 AQLERQYSGA 3322 AQLFQVFRQA 3323 AQLGDSTLKA 3324 AQLGLKLRPA 3325 AQLGLSDRRA 3326 AQLGQWSAGA 3327 AQLHLNSKSA 3328 AQLIGGSGSA 3329 AQLKHSNDKA 3330 AQLKNSPWDA 3331 AQLLHGESVA 3332 AQLLSSRAAA 3333 AQLMHSARVA 3334 AQLMPPMGRA 3335 AQLPNTLSMA 3336 AQLRTIRIAA 3337 AQLSREYNSA 3338 AQLSRVMASA 3339 AQLSSSLRSA 3340 AQLTPGYKTA 3341 AQLVTFVPEA 3342 AQLVTPNPVA 3343 AQMASLLPEA 3344 AQMASLLQEA 3345 AQMASLLTEA 3346 AQMAYLLPEA 3347 AQMDRVTSPA 3348 AQMDSLLPEA 3349 AQMGGLLLSA 3350 AQMHGPGSNA 3351 AQMNAFNTHA 3352 AQMRSAYPAA 3353 AQMSRTRLPA 3354 AQMTYSQPKA 3355 AQNGKILVPA 3356 AQNRSLPHQA 3357 AQNSANTTCA 3358 AQPGIPFHGA 3359 AQPGQSFPAA 3360 AQPGYHLTSA 3361 AQPLRMGNLA 3362 AQPSGITALA 3363 AQPTRLVGGA 3364 AQPVRNDRLA 3365 AQQEKSSTPA 3366 AQQLVALPFA 3367 AQQPSRAAWA 3368 AQQPSYISTA 3369 AQQSANTMAA 3370 AQQSFPRATA 3371 AQQSPVAVRA 3372 AQQTTSWSEA 3373 AQQTTVRTDA 3374 AQQVQFRFEA 3375 AQRALNKSDA 3376 AQRDLAHTQA 3377 AQRDLEHSQA 3378 AQRDRDARSA 3379 AQREFTPMDA 3380 AQRGFPASTA 3381 AQRGYDLSPA 3382 AQRGYDLSTA 3383 AQRGYENEKA 3384 AQRHLELKEA 3385 AQRLAVQAKA 3386 AQRLHSSATA 3387 AQRLRQQEDA 3388 AQRLSGQSSA 3389 AQRNSYLSDA 3390 AQRQAVAQSA 3391 AQRQGPDLLA 3392 AQRRGDQGQA 3393 AQRSASGIQA 3394 AQRSGSPQPA 3395 AQRSIMKGQA 3396 AQRSIMQGKA 3397 AQRSLASVTA 3398 AQRSYPSTSA 3399 AQRTSDLLQA 3400 AQRTSSMSEA 3401 AQRYLGNSLA 3402 AQRYTNQVPA 3403 AQRYVNNSAA 3404 AQSFSSEQLA 3405 AQSGIRDARA 3406 AQSGKSIAGA 3407 AQSGNHFGKA 3408 AQSGRVVTLA 3409 AQSIQYLDYA 3410 AQSIQYSHTA 3411 AQSISGVAMA 3412 AQSKSAITWA 3413 AQSKTSASQA 3414 AQSLAKDLSA 3415 AQSLGTGLQA 3416 AQSLQHLDWA 3417 AQSMDWPPSA 3418 AQSMPNSPMA 3419 AQSMSARGLA 3420 AQSNLSYARA 3421 AQSNSYLDSA 3422 AQSQAVAQSA 3423 AQSQLFGLRA 3424 AQSQLVGLRA 3425 AQSQSGTAWA 3426 AQSRGQDKAA 3427 AQSRMLPTSA 3428 AQSRVLSHQA 3429 AQSSMSRLVA 3430 AQSTLTVLPA 3431 AQSVQYSTSA 3432 AQSWVGPAVA 3433 AQSYASSYAA 3434 AQTKSFSSAA 3435 AQTLARPRIA 3436 AQTLPFISSA 3437 AQTMGGQYSA 3438 AQTMSGSMVA 3439 AQTMVSARPA 3440 AQTNLNFNLA 3441 AQTPAFINLA 3442 AQTPRQLASA 3443 AQTQGSSGWA 3444 AQTQSIDPSA 3445 AQTQVPSGAA 3446 AQTRGDSVGA 3447 AQTSQMYQTA 3448 AQTSVMEQRA 3449 AQTVETHMRA 3450 AQTVSVHVSA 3451 AQTVYQQAPA 3452 AQVGAYADVA 3453 AQVIGNSSAA 3454 AQVLRAQSQA 3455 AQVLRMKGIA 3456 AQVPRYMRPA 3457 AQVPSVRSSA 3458 AQVQYLNSHA 3459 AQVREFRLTA 3460 AQVSRHVTSA 3461 AQVTRSLLSA 3462 AQVVKNSSVA 3463 AQVVVLTTAA 3464 AQVVYSEGRA 3465 AQWGKSDLTA 3466 AQWPQFMSAA 3467 AQWQMKQVSA 3468 AQYGAAPGVA 3469 AQYKGFEKVA 3470 AQYKSGGLTA 3471 AQYMQGALHA 3472 AQYPSQTVTA 3473 AQYSAHVSQA 3474 AQYSLAGSSA 3475 AQYSLGQATA 3476 AQYVRSPRKA 3477 ARAYSTDVRM 3478 ARVRADSHLA 3479 ASLSAVAAVA 3480 AVTRAVQNSA 3481 CPAAAVLAAA 3482 DDSRAVGLRA 3483 DRQPAVHMTA 3484 EGAFAVLPYA 3485 EQAFSTSQFT 3486 ETFNAVRNSA 3487 FAGSAVMTMA 3488 FALPAVPGSA 3489 FLAPAVSTKA 3490 FRSVAVENVA 3491 FRYNAVGEGA 3492 FSLPAVPNIA 3493 FVSNAVQGKA 3494 FVTSAVTEPA 3495 GALNAVTGVD 3496 GASRAVVLSA 3497 GDHRAVAARA 3498 GDNFAVSGMA 3499 GGGHAVVLAA 3500 GGGHAVVQAA 3501 GGGNAVVLAA 3502 GLSSAVIAQA 3503 GMDRAVQTQA 3504 GNLSAVIIQA 3505 GPAMAVVGVA 3506 GPRQAVAGIA 3507 GQYAAVSSYA 3508 GRGHAVVLAA 3509 GRRQAVHSEA 3510 GSGSAVVTQA 3511 GTRSAVAALA 3512 GTSYAVGGQA 3513 HAQLAVVGEA 3514 HSPAAVGLQA 3515 HTATAVGLQA 3516 IASLAVSQVA 3517 ISGHAVSTAA 3518 KQYGAVEGQA 3519 KSSAAVHATA 3520 LAAVAVQSPA 3521 LAENAVKLMA 3522 LDSRAVLSSA 3523 LGRGAVLDSA 3524 LGRGAVLDTA 3525 LGRGAVLDTV 3526 LGRGAVLGTA 3527 LGRGAVLVTA 3528 LGRGPVLDTA 3529 LGRGSVLDTA 3530 LGRGVVLDTA 3531 LLAPAVSSKA 3532 LLYPAVVLEA 3533 LRRGAVLDTA 3534 LSPGAVVTSA 3535 LTNGAGRDRA 3536 LTNGAIRDRA 3537 LTNGAVGDRA 3538 LTNGAVRDQA 3539 LTNGAVRGRA 3540 LVNSAVLRNA 3541 LVPSAVVSKA 3542 MAVKAVPWTA 3543 MGRSAVNPVA 3544 MPNRAVIDSA 3545 MRSRAVDPVA 3546 MSLHAVTHSA 3547 NASLAVSTAA 3548 NRSDAVRMVA 3549 NTERAVILEA 3550 NTRGAVGSAA 3551 NTVSAVILEA 3552 PAGSAVVSPA 3553 PFNSAVSSSA 3554 PSSYAVSHVA 3555 PTLTAVAHAA 3556 QGQAAVGIYA 3557 QLNRAVNSGA 3558 QMSRAVSDYA 3559 RDSMAVGNVA 3560 RFGSAVGLTA 3561 RIVNAVKAQA 3562 RLDQAVSTSA 3563 RLLPAVSNDA 3564 RLSEAVSMTA 3565 RLSKAVAAGA 3566 RNDVAVVHTA 3567 RNSYAVSEAA 3568 RPSNAVSVHA 3569 RTGKAVDTVA 3570 RTPDAVHMLA 3571 SANLAVTLLA 3572 SAVRAVTWDA 3573 SEIGAVYGSA 3574 SGESAVVSVA 3575 SGLKAVGNPA 3576 SGLLAVSPPA 3577 SGSLAVGSMA 3578 SGTRAVSPSA 3579 SGYTAVASGA 3580 SLNAAVHSGA 3581 SLNTAVASLA 3582 SLSYAVDMRA 3583 SLVGALAQMA 3584 SLVGAVSQMA 3585 SMRQAVSQYA 3586 SQAHMQASVT 3587 SQNNAVVSYA 3588 SRSKAVSWEA 3589 SRYSAVPREA 3590 SSSPAVTRSA 3591 STESAVSDRA 3592 SVIGAVYGYA 3593 SVNKAVASLA 3594 SVNTAVASMA 3595 SVSKAVSLGA 3596 TARYAVAQQA 3597 TFRSAVELRA 3598 TFYSAVKLGA 3599 TPIQAVRESA 3600 TQHIDSMRPA 3601 TQSKTTLTLA 3602 TQYGAVECQA 3603 TQYGAVEGRA 3604 TTALAVGDNA 3605 TVLSAVSGGA 3606 VAVHAVGSVA 3607 VDTRAVGHQA 3608 VGGAAVGTTA 3609 VKERAVSQSA 3610 VMNGAVRLLA 3611 VPGSAVIAAA 3612 VSTYAVSMQA 3613 VTGKAVTGVA 3614 VTRGAVEAHA 3615 VVESAVVGLA 3616 VVNVAVLHVA 3617 VVSSAVGRTA 3618 YLVGAVAQMA 3619 YNRTAVSSEA 3620 YRSTAVEGYA 3621 AQASFNDTRA 3622

TABLE 2 Exemplary Peptide, e.g., Targeting Peptide Sequences SEQ Amino SEQ ID Acid ID Peptide NO: Sequence NO: Nucleotide Sequence 1 3648 PLNGAVHLY 3660 ccgcttaatggtgccgtccatc tttat 2 3649 RDSPKGW 3661 cgtgattctccgaagggttggca 3 3650 YSTDVRM 3662 tattctacggatgtgaggatgca 4 3651 IVMNSLK 3663 attgttatgaattcgttgaaggc 5 3652 RESPRGL 3664 cgggagagtcctcgtgggctgca 6 3653 SFNDTRA 3665 agttttaatgatactagggctca 7 3654 GGTLAVVSL 3666 ggtggtacgttggccgtcgtgt cgctt 8 3655 YGLPKGP 3667 tatgggttgccgaagggtcct 9 3656 STGTLRL 3668 tcgactgggacgcttcggctt 10 3657 YSTDERM 3669 tattcgacggatgagaggatg 11 3658 YSTDERK 3670 tattcgacggatgagaggaag 12 3659 YVSSVKM 3671 tatgtttcgtctgttaagatg

In some embodiments, the peptide, e.g., targeting peptide, comprises at least 3, 4, 5, 6, 7, 8, or 9 consecutive amino acids from the amino acid sequence of any of SEQ ID NO: 1725-3622. In some embodiments, the peptide comprises at least 3, 4, 5, 6, 7, 8, or 9 consecutive amino acids from the amino acid sequence of any of SEQ ID NO: 3648-3659.

In some embodiments, the 3 consecutive amino acids comprise PLN. In some embodiments, the 4 consecutive amino acids comprise PLNG (SEQ ID NO: 3678). In some embodiments, the 5 consecutive amino acids comprise PLNGA (SEQ ID NO: 3679). In some embodiments, the 6 consecutive amino acids comprise PLNGAV (SEQ ID NO: 3680). In some embodiments, the 7 consecutive amino acids comprise PLNGAVH (SEQ ID NO: 3681). In some embodiments, the 8 consecutive amino acids comprise PLNGAVHL (SEQ ID NO: 3682). In some embodiments, the 9 consecutive amino acids comprise PLNGAVHLY (SEQ ID NO: 3648).

In some embodiments, the four consecutive amino acids comprise NGAV (SEQ ID NO: 3683). In some embodiments, the four consecutive amino acids comprise GAVH (SEQ ID NO: 3684). In some embodiments, the five consecutive amino acids comprise NGAVH (SEQ ID NO: 3685). In some embodiments, the five consecutive amino acids comprise GAVHL (SEQ ID NO: 3686). In some embodiments, the five consecutive amino acids comprise AVHLY (SEQ ID NO: 3687). In some embodiments, the six consecutive amino acids comprise NGAVHL (SEQ ID NO: 3688). In some embodiments, the seven consecutive amino acids comprise NGAVHLY (SEQ ID NO: 3689).

In some embodiments, the 3 consecutive amino acids comprise YST. In some embodiments, the 4 consecutive amino acids comprise YSTD (SEQ ID NO: 3690). In some embodiments, the 5 consecutive amino acids comprise YSTDE (SEQ ID NO: 3691). In some embodiments, the 5 consecutive amino acids comprise YSTDV (SEQ ID NO: 3700). In some embodiments, the 6 consecutive amino acids comprise YSTDER (SEQ ID NO: 3692). In some embodiments, the 6 consecutive amino acids comprise YSTDVR (SEQ ID NO: 3701). In some embodiments, the 7 consecutive amino acids comprise YSTDERM (SEQ ID NO: 3657). In some embodiments, the 7 consecutive amino acids comprise YSTDERK (SEQ ID NO: 3658). In some embodiments, the 7 consecutive amino acids comprise YSTDVRM (SEQ ID NO: 3650).

In some embodiments, the 3 consecutive amino acids comprise IVM. In some embodiments, the 4 consecutive amino acids comprise IVMN (SEQ ID NO: 3693). In some embodiments, the 5 consecutive amino acids comprise IVMNS (SEQ ID NO: 3694). In some embodiments, the 6 consecutive amino acids comprise IVMNSL (SEQ ID NO: 3695). In some embodiments, the 7 consecutive amino acids comprise IVMNSLK (SEQ ID NO: 3651).

In some embodiments, the peptide, e.g., targeting peptide, comprises an amino acid sequence comprising at least one, two, or three but no more than four modifications, e.g., substitutions, relative to the amino acid sequence of any of SEQ ID NO: 1725-3622. In some embodiments, the peptide, e.g., targeting peptide, comprises an amino acid sequence comprising at least one, two, or three but no more than four modifications, e.g., substitutions, relative to the amino acid sequence of any of SEQ ID NO: 3648-3659.

In some embodiments, the peptide, e.g., targeting peptide comprises the amino acid sequence of PLNGAVHLY (SEQ ID NO: 3648), or an amino acid sequence having at least one, two, or three but no more than four modifications, e.g., substitutions, relative to the amino acid sequence of PLNGAVHLY (SEQ ID NO: 3648), optionally wherein position 7 is H.

In some embodiments, the peptide, e.g., targeting peptide comprises the amino acid sequence of RDSPKGW (SEQ ID NO: 3649), or an amino acid sequence having at least one, two, or three modifications but no more than four modifications, e.g., substitutions, relative to the amino acid sequence of RDSPKGW (SEQ ID NO: 3649).

In some embodiments, the peptide, e.g., targeting peptide comprises the amino acid sequence of IVMNSLK (SEQ ID NO: 3651), or an amino acid sequence having at least one, two, or three modifications but no more than four modifications, e.g., substitutions, relative to the amino acid sequence of IVMNSLK (SEQ ID NO: 3651).

In some embodiments, the peptide, e.g., targeting peptide comprises the amino acid sequence of YSTDVRM (SEQ ID NO: 3650), or an amino acid sequence having at least one, two, or three modifications but no more than four modifications, e.g., substitutions, relative to the amino acid sequence of YSTDVRM (SEQ ID NO: 3650).

In some embodiments, the peptide, e.g., targeting peptide comprises the amino acid sequence of RESPRGL (SEQ ID NO: 3652), or a sequence having at least one, two, or three modifications but no more than four modifications, e.g., substitutions, relative to the amino acid sequence of RESPRGL (SEQ ID NO: 3652).

In some embodiments, the peptide, e.g., targeting peptide comprises the amino acid sequence of any of SEQ ID NO: 1725-3622. In some embodiments, the peptide comprises the amino acid sequence of any of SEQ ID NO: 3648-3659.

In some embodiments, the peptide, e.g., targeting peptide, may comprise an amino acid sequence with 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identity to any of the sequences shown in Table 1 or Table 2.

In some embodiments, the peptide, e.g., targeting peptide, comprises the amino acid sequence of SEQ ID NO: 3648. In some embodiments, the peptide, e.g., targeting peptide, comprises the amino acid sequence of SEQ ID NO: 3649. In some embodiments, the peptide, e.g., targeting peptide, comprises the amino acid sequence of SEQ ID NO: 3650. In some embodiments, the peptide, e.g., targeting peptide, comprises the amino acid sequence of SEQ ID NO: 3651. In some embodiments, the peptide, e.g., targeting peptide, comprises the amino acid sequence of SEQ ID NO: 3652. In some embodiments, the peptide, e.g., targeting peptide, comprises the amino acid sequence of SEQ ID NO: 3653. In some embodiments, the peptide, e.g., targeting peptide, comprises the amino acid sequence of SEQ ID NO: 3654. In some embodiments, the peptide, e.g., targeting peptide, comprises the amino acid sequence of SEQ ID NO: 3655. In some embodiments, the peptide, e.g., targeting peptide, comprises the amino acid sequence of SEQ ID NO: 3656. In some embodiments, the peptide, e.g., targeting peptide, comprises the amino acid sequence of SEQ ID NO: 3657. In some embodiments, the peptide, e.g., targeting peptide, comprises the amino acid sequence of SEQ ID NO: 3658. In some embodiments, the peptide, e.g., targeting peptide, comprises the amino acid sequence of SEQ ID NO: 3659.

In some embodiments, the peptide, e.g., targeting peptide, may comprise SEQ ID NO: 1725. In some embodiments, the peptide, e.g., targeting peptide, may comprise SEQ ID NO: 1726. In some embodiments, the peptide, e.g., targeting peptide, may comprise SEQ ID NO: 1729. In some embodiments, the peptide, e.g., targeting peptide, may comprise SEQ ID NO: 1760. In some embodiments, the peptide, e.g., targeting peptide, may comprise SEQ ID NO: 1769. In some embodiments, the peptide, e.g., targeting peptide, may comprise SEQ ID NO: 3622. In some embodiments, the peptide, e.g., targeting peptide, may comprise SEQ ID NO: 1798. In some embodiments, the peptide, e.g., targeting peptide, may comprise SEQ ID NO: 1785. In some embodiments, the peptide, e.g., targeting peptide, may comprise SEQ ID NO: 1767. In some embodiments, the peptide, e.g., targeting peptide, may comprise SEQ ID NO: 1734. In some embodiments, the peptide, e.g., targeting peptide, may comprise SEQ ID NO: 1737. In some embodiments, the peptide, e.g., targeting peptide, may comprise SEQ ID NO: 1819.

In some embodiments, a peptide, e.g., targeting peptide, may comprise 4 or more contiguous amino acids of any of the peptides, e.g., targeting peptides, disclosed herein. In some embodiments the peptide, e.g., targeting peptide, may comprise 4 contiguous amino acids of any of the sequences as set forth in Table 1 or Table 2. In some embodiments the peptide, e.g., targeting peptide, may comprise 5 contiguous amino acids of any of the sequences as set forth in Table 1 or 2. In some embodiments the peptide, e.g., targeting peptide, may comprise 6 contiguous amino acids of any of the sequences as set forth in Table 1 or 2.

In some embodiments, the peptide, e.g., targeting peptide, comprises an amino acid sequence encoded by a nucleotide sequence described herein, e.g., a nucleotide sequence of Table 2. In some embodiments, the peptide comprises an amino acid sequence encoded by a nucleotide sequence comprising at least one, two, three, four, five, six, or seven modifications but no more than ten modifications of the nucleotide sequences of any of SEQ ID NOs: 3660-3671. In some embodiments, the peptide comprises an amino acid sequence encoded by the nucleotide sequence of any one of SEQ ID NOs: 3660-3671, or a nucleotide sequence substantially identical (e.g., having at least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence identity) thereto.

In some embodiments, the peptide, e.g., targeting peptide comprises an amino acid sequence encoded by a nucleotide sequence comprising at least one, two, three, four, five, six, or seven modifications but no more than ten modifications of the nucleotide sequence of SEQ ID NO: 3660. In some embodiments, the peptide comprises an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 3660, or a nucleotide sequence substantially identical (e.g., having at least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence identity) thereto.

In some embodiments, the peptide, e.g., targeting peptide, comprises an amino acid sequence encoded by a nucleotide sequence comprising at least one, two, three, four, five, six, or seven modifications but no more than ten modifications of the nucleotide sequence of SEQ ID NO: 3663. In some embodiments, the peptide comprises an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 3663, or a nucleotide sequence substantially identical (e.g., having at least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence identity) thereto.

In some embodiments, the nucleotide sequence encoding a peptide, e.g., targeting peptide, described herein e.g., peptide 1-12, comprises a nucleotide sequence described herein, e.g., as described in Table 2. In some embodiments, the nucleic acid sequence encoding a peptide described herein comprises the nucleotide sequence of any of SEQ ID NOs: 3660-3671, or a nucleotide sequence substantially identical (e.g., having at least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence identity) thereto. In some embodiments, the nucleic acid sequence encoding a peptide described herein comprises a nucleotide sequence comprising at least one, two, three, four, five, six, or seven modifications but no more than ten modifications of the nucleotide sequences of any of SEQ ID NOs: 3660-3671. In some embodiments, the nucleotide sequence encoding a peptide, e.g., targeting peptide, described herein is isolated, e.g., recombinant.

In some embodiments the nucleotide sequence encoding a peptide, e.g., targeting peptide, described herein comprises a nucleotide sequence comprising at least one, two, three, four, five, six, or seven modifications but no more than ten modifications of the nucleotide sequence of SEQ ID NO: 3660. In some embodiments the nucleic acid sequence encoding a peptide described herein comprises a nucleotide sequence comprising the nucleotide sequence of SEQ ID NO: 3660, or a nucleotide sequence substantially identical (e.g., having at least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence identity) thereto.

In some embodiments, the nucleic acid encoding a peptide described herein comprises a nucleotide sequence comprising at least one, two, three, four, five, six, or seven modifications but no more than ten modifications of the nucleotide sequence of SEQ ID NO: 3663. In some embodiments the nucleic acid encoding a peptide described herein comprises a nucleotide sequence comprising the nucleotide sequence of SEQ ID NO: 3663, or a nucleotide sequence substantially identical (e.g., having at least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence identity) thereto.

In some embodiments, an AAV particle of the disclosure comprises an AAV capsid, e.g., and AAV capsid variant, with a peptide, e.g., targeting peptide, insert (e.g., an AAV capsid variant), wherein the peptide, e.g., targeting peptide, has an amino acid sequence as set forth in any of Table 1 or Table 2.

In some embodiments, an AAV particle of the disclosure comprises an AAV capsid polypeptide, e.g., an AAV capsid variant, with a peptide, e.g., targeting peptide, insert (e.g., an AAV capsid variant), wherein the peptide has an amino acid having at least one, two, or three modifications but no more than four modifications, e.g., substitutions, relative to the amino acid sequence in any of Table 1 or Table 2.

In some embodiments, the AAV particle of the disclosure comprises an AAV capsid polypeptide, e.g., an AAV capsid variant, with a peptide, e.g., targeting peptide, insert (e.g., an AAV capsid variant), wherein the peptide, e.g., targeting peptide, has an amino acid sequence comprising at least 3, 4, 5, 6, 7, 8, or 9 contiguous amino acids of any of the sequences as set forth in any of Table 1 or 2.

In some embodiments, the AAV particle of the disclosure comprises an AAV capsid, e.g., an AAV capsid variant, with a peptide, e.g., a targeting peptide, insert (e.g., an AAV capsid variant), wherein the peptide, e.g., targeting peptide, has an amino acid sequence substantially identical thereto (e.g., having at least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence identity) to any of the sequences as set forth in any of Table 1 or 2.

In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 1 to the amino acid at position 2 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 1 to the amino acid at position 3 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 2 to the amino acid at position 3 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 1 to the amino acid at position 4 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide may include the amino acid at position 2 to the amino acid at position 4 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide may include the amino acid at position 3 to the amino acid at position 4 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 1 to the amino acid at position 5 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 2 to the amino acid at position 5 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 3 to the amino acid at position 5 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 4 to the amino acid at position 5 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 1 to the amino acid at position 6 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 2 to the amino acid at position 6 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 3 to the amino acid at position 6 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 4 to the amino acid at position 6 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 5 to the amino acid at position 6 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 1 to the amino acid at position 7 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 2 to the amino acid at position 7 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 3 to the amino acid at position 7 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 4 to the amino acid at position 7 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 5 to the amino acid at position 7 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 6 to the amino acid at position 7 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 1 to the amino acid at position 8 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 2 to the amino acid at position 8 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 3 to the amino acid at position 8 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 4 to the amino acid at position 8 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 5 to the amino acid at position 8 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 6 to the amino acid at position 8 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 7 to the amino acid at position 8 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 1 to the amino acid at position 9 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 2 to the amino acid at position 9 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 3 to the amino acid at position 9 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 4 to the amino acid at position 9 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 5 to the amino acid at position 9 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 6 to the amino acid at position 9 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 7 to the amino acid at position 9 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 8 to the amino acid at position 9 of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 1 to the amino acid at position 10 of SEQ ID NO: 1725-3622. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 2 to the amino acid at position 10 of SEQ ID NO: 1725-3622. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 3 to the amino acid at position 10 of SEQ ID NO: 1725-3622. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 4 to the amino acid at position 10 of SEQ ID NO: 1725-3622. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 5 to the amino acid at position 10 of SEQ ID NO: 1725-3622. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 6 to the amino acid at position 10 of SEQ ID NO: 1725-3622. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 7 to the amino acid at position 10 of SEQ ID NO: 1725-3622. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 8 to the amino acid at position 10 of SEQ ID NO: 1725-3622. In some embodiments, the peptide, e.g., targeting peptide, may include the amino acid at position 9 to the amino acid at position 10 of SEQ ID NO: 1725-3622.

The present disclosure also provides a nucleic acid or polynucleotide encoding any of the above described peptides, e.g., targeting peptides, and AAV capsid polypeptides, e.g., AAV capsid variants, AAV particles, vectors, and cells comprising the same.

In some embodiments, an insertion of a peptide, e.g., targeting peptide, into a parent AAV capsid, e.g., a parental sequence, results in a combination insertion/replacement as compared to the parent amino acid sequence. In some embodiments, all the amino acids of a peptide, e.g., targeting peptide, are inserted into the parent AAV capsid sequence. In some embodiments, one amino acid of the peptide, e.g., targeting peptide, replaces one amino acid in the parent AAV capsid while the remaining amino acids of the peptide, e.g., targeting peptide, are inserted into the parent AAV capsid sequence. In some embodiments, two amino acids of the peptide, e.g., targeting peptide, replace two amino acids in the parent AAV capsid while the remaining amino acids of the peptide, e.g., targeting peptide, are inserted into the parent AAV capsid sequence. In some embodiments, three amino acids of the peptide, e.g., targeting peptide, replace three amino acids in the parent AAV capsid while the remaining amino acids of the peptide, e.g., targeting peptide, are inserted into the parent AAV capsid sequence. In some embodiments, four amino acids of the peptide, e.g., targeting peptide, replace four amino acids in the parent AAV capsid while the remaining amino acids of the peptide, e.g., targeting peptide, are inserted into the parent AAV capsid sequence. In some embodiments, five amino acids of the peptide, e.g., targeting peptide, replace five amino acids in the parent AAV capsid while the remaining amino acids of the peptide, e.g., targeting peptide, are inserted into the parent AAV capsid sequence. In some embodiments, six amino acids of the peptide, e.g., targeting peptide, replace six amino acids in the parent AAV capsid while the remaining amino acids of the peptide, e.g., targeting peptide, are inserted into the parent AAV capsid sequence. In some embodiments, seven amino acids of the peptide, e.g., targeting peptide, replace seven amino acids in the parent AAV capsid while the remaining amino acids of the peptide, e.g., targeting peptide, are inserted into the parent AAV capsid sequence. In some embodiments, eight amino acids of the peptide, e.g., targeting peptide, replace eight amino acids in the parent AAV capsid while the remaining amino acids of the peptide, e.g., targeting peptide, are inserted into the parent AAV capsid sequence. In some embodiments, nine amino acids of the peptide, e.g., targeting peptide, replace nine amino acids in the parent AAV capsid while the remaining amino acids of the peptide, e.g., targeting peptide, are inserted into the parent AAV capsid sequence.

In some embodiments, one amino acid of the peptide, e.g., targeting peptide, is inserted into the parent AAV capsid, e.g., parental sequence, while the remaining amino acids of the peptide, e.g., targeting peptide, replace corresponding amino acids in the parent AAV capsid sequence. In some embodiments, two amino acids of the peptide, e.g., targeting peptide, are inserted into the parent AAV capsid while the remaining amino acids of the peptide, e.g., targeting peptide, replace corresponding amino acids in the parent AAV capsid sequence. In some embodiments, three amino acids of the peptide, e.g., targeting peptide, are inserted into the parent AAV capsid while the remaining amino acids of the peptide, e.g., targeting peptide, replace corresponding amino acids in the parent AAV capsid sequence. In some embodiments, four amino acids of the peptide, e.g., targeting peptide, are inserted into the parent AAV capsid while the remaining amino acids of the peptide, e.g., targeting peptide, replace corresponding amino acids in the parent AAV capsid sequence. In some embodiments, five amino acids of the peptide, e.g., targeting peptide, are inserted into the parent AAV capsid while the remaining amino acids of the peptide, e.g., targeting peptide, replace corresponding amino acids in the parent AAV capsid sequence. In some embodiments, six amino acids of the peptide, e.g., targeting peptide, are inserted into the parent AAV capsid while the remaining amino acids of the peptide, e.g., targeting peptide, replace corresponding amino acids in the parent AAV capsid sequence. In some embodiments, seven amino acids of the peptide, e.g., targeting peptide, are inserted into the parent AAV capsid while the remaining amino acids of the peptide, e.g., targeting peptide, replace corresponding amino acids in the parent AAV capsid sequence. In some embodiments, eight amino acids of the peptide, e.g., targeting peptide, are inserted into the parent AAV capsid while the remaining amino acids of the peptide, e.g., targeting peptide, replace corresponding amino acids in the parent AAV capsid sequence. In some embodiments, nine amino acids of the peptide, e.g., targeting peptide, are inserted into the parent AAV capsid while the remaining amino acids of the peptide, e.g., targeting peptide, replace corresponding amino acids in the parent AAV capsid sequence.

In some embodiments, certain amino acids of the peptide, e.g., targeting peptide, may be anchored and or retained as in the original parent AAV capsid sequence, e.g., parental sequence. In certain embodiments, these anchored amino acids are centrally located in the peptide, e.g., targeting peptide, resulting in a split insertion-anchor-insertion design. Similarly, as a non-limiting example, the insertion of a peptide, e.g., targeting peptide, may result in a split insertion-replacement-insertion design. As a non-limiting example, the insertion of a peptide, e.g., targeting peptide, into a parent AAV capsid sequence may result in a split replacement-insertion-replacement design. As a non-limiting example, a split design peptide, e.g., targeting peptide, may be as shown in FIG. 4 (e.g., TNHQSXXXXAVXXXAQAQT (SEQ ID NO: 3699)).

In some embodiments, an insertion of a peptide, e.g., targeting peptide, into the parent AAV capsid sequence, e.g., parental sequence, may result in the replacement or mutation of at least one amino acid of the parent AAV capsid. In certain embodiments, the first amino acid (N-terminal) of the peptide, e.g., targeting peptide, replaces an amino acid in the parent AAV capsid sequence. In certain embodiments, the first two amino acids (N-terminal) of the peptide, e.g., targeting peptide, replace two amino acids in the parent AAV capsid sequence. In certain embodiments, the first three amino acids (N-terminal) of the peptide, e.g., targeting peptide, replace three amino acids in the parent AAV capsid sequence. In certain embodiments, the first four amino acids (N-terminal) of the peptide, e.g., targeting peptide, replace four amino acids in the parent AAV capsid sequence. In certain embodiments, the first five amino acids (N-terminal) of the peptide, e.g., targeting peptide, replace five amino acids in the parent AAV capsid sequence. In certain embodiments, the first six amino acids (N-terminal) of the peptide, e.g., targeting peptide, replace six amino acids in the parent AAV capsid sequence. In certain embodiments, the first seven amino acids (N-terminal) of the peptide, e.g., targeting peptide, replace seven amino acids in the parent AAV capsid sequence. In certain embodiments, the first eight amino acids (N-terminal) of the peptide, e.g., targeting peptide, replace eight amino acids in the parent AAV capsid sequence. In certain embodiments, the first nine amino acids (N-terminal) of the peptide, e.g., targeting peptide, replace nine amino acids in the parent AAV capsid sequence. In certain embodiments, the first ten amino acids (N-terminal) of the peptide, e.g., targeting peptide, replace ten amino acids in the parent AAV capsid sequence.

In certain embodiments, the last amino acid (C-terminal) of the peptide, e.g., targeting peptide, replaces an amino acid in the parent AAV capsid sequence. In certain embodiments, the last two amino acids (C-terminal) of the peptide, e.g., targeting peptide, replace two amino acids in the parent AAV capsid sequence. In certain embodiments, the last three amino acids (C-terminal) of the peptide, e.g., targeting peptide, replace three amino acids in the parent AAV capsid sequence. In certain embodiments, the last four amino acids (C-terminal) of the peptide, e.g., targeting peptide, replace four amino acids in the parent AAV capsid sequence. In certain embodiments, the last five amino acids (C-terminal) of the peptide, e.g., targeting peptide, replace five amino acids in the parent AAV capsid sequence. In certain embodiments, the last six amino acids (C-terminal) of the peptide, e.g., targeting peptide, replace six amino acids in the parent AAV capsid sequence. In certain embodiments, the last seven amino acids (C-terminal) of the peptide, e.g., targeting peptide, replace seven amino acids in the parent AAV capsid sequence. In certain embodiments, the last eight amino acids (C-terminal) of the peptide, e.g., targeting peptide, replace eight amino acids in the parent AAV capsid sequence. In certain embodiments, the last nine amino acids (C-terminal) of the peptide, e.g., targeting peptide, replace nine amino acids in the parent AAV capsid sequence. In certain embodiments, the last ten amino acids (C-terminal) of the peptide, e.g., targeting peptide, replace ten amino acids in the parent AAV capsid sequence.

In certain embodiments, the first (N-terminal) and last (C-terminal) amino acids of the peptide, e.g., targeting peptide, may replace amino acids in the parent AAV capsid sequence, e.g., parental sequence. In certain embodiments, the first two (N-terminal) and last two (C-terminal) amino acids of the peptide, e.g., targeting peptide, may replace amino acids in the parent AAV capsid sequence. In certain embodiments, the first three (N-terminal) and last three (N-terminal) amino acids of the peptide, e.g., targeting peptide, may replace amino acids in the parent AAV capsid sequence. In certain embodiments, the replacements are asymmetrical in terms of N-terminal and C-terminal replacements and may be any combination of the any of the above.

In certain embodiments, one amino acid of the peptide, e.g., targeting peptide, replaces an amino acid in the parent AAV capsid sequence, e.g., parental sequence. In certain embodiments, two amino acids of the peptide, e.g., targeting peptide, replace two amino acids in the parent AAV capsid sequence. In certain embodiments, three amino acids of the peptide, e.g., targeting peptide, replace three amino acids in the parent AAV capsid sequence. In certain embodiments, four amino acids of the peptide, e.g., targeting peptide, replace four amino acids in the parent AAV capsid sequence. In certain embodiments, five amino acids of the peptide, e.g., targeting peptide, replace five amino acids in the parent AAV capsid sequence. In certain embodiments, six amino acids of the peptide, e.g., targeting peptide, replace six amino acids in the parent AAV capsid sequence. In certain embodiments, seven amino acids of the peptide, e.g., targeting peptide, replace seven amino acids in the parent AAV capsid sequence. In certain embodiments, eight amino acids of the peptide, e.g., targeting peptide, replace eight amino acids in the parent AAV capsid sequence. In certain embodiments, nine amino acids of the peptide, e.g., targeting peptide, replace nine amino acids in the parent AAV capsid sequence. In certain embodiments, ten amino acids of the peptide, e.g., targeting peptide, replace ten amino acids in the parent AAV capsid sequence. In certain embodiments, all amino acids of the peptide, e.g., targeting peptide, replace the same number of amino acids in the parent AAV capsid sequence.

In some embodiments, the AAV particle of the disclosure may comprise a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, wherein said polynucleotide further comprising a nucleic acid insert, e.g., a targeting nucleic acid insert, wherein the nucleic acid insert has a nucleotide sequence substantially comprising any of those as described in Hanlon et al., 2019 (Hanlon et al., Mol Ther Methods Clin Dev. 2019 Oct. 23; 15:320-332, the contents of which are herein incorporated by reference in its entirety). As a non-limiting the nucleic acid insert, e.g., targeting nucleic acid insert, has a nucleotide sequence substantially comprising AAV-S. As a non-limiting example, the targeting nucleic acid insert has a nucleotide sequence substantially comprising AAV-F.

The AAV particle of the disclosure comprising a nucleic acid insert, e.g., a targeting nucleic acid insert, may have a polynucleotide sequence encoding a capsid polypeptide with 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more, identity to the parent capsid sequence.

The AAV particle of the disclosure comprising a peptide, e.g., a targeting peptide, insert, may have an amino acid sequence with 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more, identity to the parent capsid sequence.

AAV Capsid Polypeptide, e.g., AAV Capsid Variant

In some embodiments, a peptide, e.g., targeting peptide, is inserted, included or otherwise incorporated into a polypeptide. In some embodiments, such a polypeptide may be referred to as the “parental polypeptide” or “starting polypeptide” or “parental amino acid sequence” and such an insert may be referred to as “targeting peptide insert”, “peptide insert” or “amino acid sequence insert”. In some embodiments, an AAV capsid polypeptide, e.g., an AAV capsid variant described herein may comprise a peptide, e.g., targeting peptide, insert and a polypeptide, e.g., a larger polypeptide described herein. In some embodiments, the AAV capsid polypeptide, e.g., the AAV capsid variant has an amino acid sequence that is longer than the parent AAV capsid. In other embodiments, the AAV capsid polypeptide, e.g., the AAV capsid variant has an amino acid sequence that is the same length as the parent AAV capsid. In some embodiments, the AAV capsid polypeptide, e.g., the AAV capsid variant has an amino acid sequence that is shorter than the parent AAV capsid.

Where a peptide, e.g., targeting peptide, including sequences of 5-50 contiguous amino acids, is inserted into a parental polypeptide, the insertion may be between two amino acids in the parental polypeptide. Insertion may also be a split insertion whereby one contiguous portion of a peptide insert is inserted between a first set of two amino acids in the parental polypeptide and a second portion of the peptide is inserted between a second set of two amino acids in the parental polypeptide, e.g., a different site. Between this first site and second site, any number of amino acids of the parental polypeptide may be retained. In some embodiments, 1, 2, 3, 4, 5, 6, 7, 8, or 9 amino acids in the parental polypeptide may be retained between the first and second peptide insert.

In some embodiments, a peptide, e.g., targeting peptide, inserts may, in whole or in part, replace one or more amino acids in the parental polypeptide. For example, a peptide insert of 4 amino acids may be inserted immediately after position 586 in the parental polypeptide wherein the last two amino acids of the peptide insert replace the amino acids at positions 587 and 588 of the parental polypeptide. Consequently, the newly formed polypeptide will have increased in length by only two amino acids, e.g., 2 inserted and 2 substituted. In some embodiments, a combinatorial insert/substitutional (i/s) variant may comprise one or more amino acid inserts and one or more amino acid substitutions, e.g., each from 1 to 15 amino acids in length and from 1 to 15 in number.

A peptide, e.g., targeting peptide, may be stand-alone peptides or may be inserted into or conjugated to a parent sequence. In some embodiments, the peptides, e.g., targeting peptides, are inserted into the capsid protein of an AAV particle.

One or more peptides, e.g., targeting peptides, may be inserted into a parent AAV capsid sequence to generate the AAV particles of the disclosure.

A peptide, e.g., targeting peptide, may be inserted into a parent AAV capsid sequence in any location that results in fully functional AAV particles, e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant described herein. The peptide, e.g., targeting peptide may be inserted in VP1, VP2 and/or VP3. Numbering of the amino acid residues may differ across AAV serotypes. As used herein, amino acid positions of the parent AAV capsid sequence are described using AAV9 (SEQ ID NO: 138) as reference.

In some embodiments, the peptide, e.g., targeting peptide, is inserted in a hypervariable region of the AAV capsid sequence. Non-limiting examples of such hypervariable regions include Loop I, Loop II, Loop IV, Loop VI, and Loop VIII of the parent AAV capsid. While not wishing to be bound by theory, it is believed in some embodiments that these surface exposed loops, which may hereinafter also be referred to as surface loops, are typically unstructured and poorly conserved, making them suitable regions for insertion of peptides, e.g., targeting peptides.

In some embodiments, a peptide, e.g., targeting peptide, is inserted into Loop I. In another embodiment, the peptide, e.g., targeting peptide, is used to replace a portion, or all of Loop I. As a non-limiting example, addition of the peptide, e.g., targeting peptide, to the parent AAV capsid sequence may result in the replacement or mutation of at least one amino acid of the parent AAV capsid.

In some embodiments, a peptide, e.g., targeting peptide, is inserted into Loop II. In another embodiment, the targeting peptide is used to replace a portion, or all of Loop II. As a non-limiting example, addition of the targeting peptide to the parent AAV capsid sequence may result in the replacement or mutation of at least one amino acid of the parent AAV capsid.

In some embodiments, the peptide, e.g., targeting peptide, is inserted into Loop IV. In another embodiment, the peptide, e.g., targeting peptide, is used to replace a portion, or all of Loop IV. As a non-limiting example, addition of the peptide, e.g., targeting peptide, to the parent AAV capsid sequence may result in the replacement or mutation of at least one amino acid of the parent AAV capsid.

In some embodiments, the peptide, e.g., targeting peptide, is inserted into Loop VI. In another embodiment, the peptide, e.g., targeting peptide, is used to replace a portion, or all of Loop VI. As a non-limiting example, addition of the peptide, e.g., targeting peptide, to the parent AAV capsid sequence may result in the replacement or mutation of at least one amino acid of the parent AAV capsid.

In some embodiments, the peptide, e.g., targeting peptide, is inserted into Loop VIII. In another embodiment, the peptide, e.g., targeting peptide, is used to replace a portion, or all of Loop VIII. As a non-limiting example, addition of the peptide, e.g., targeting peptide, to the parent AAV capsid sequence may result in the replacement or mutation of at least one amino acid of the parent AAV capsid.

In some embodiments, more than one peptide, e.g., targeting peptide, is inserted into a parent AAV capsid sequence. As a non-limiting example, peptide, e.g., targeting peptide, may be inserted at both Loop IV and Loop VIII in the same parent AAV capsid sequence.

A peptide, e.g., targeting peptide, may be inserted at any amino acid position of the parent AAV capsid sequence, such as, but not limited to, between amino acids at positions 586-592, 588-589, 586-589, 452-458, 262-269, 464-473, 491-495, 546-557 and/or 659-668.

In some embodiments, the peptide, e.g., targeting peptide, is inserted into a parent AAV capsid sequence between amino acids at positions 588 and 589 (Loop VIII). In some embodiments, the parent AAV capsid is AAV9 (SEQ ID NO: 138). In some embodiments, the parent AAV capsid is K449R AAV9 (SEQ ID NO: 11).

In some embodiments, the peptide, e.g., targeting peptide, is inserted into a parent AAV capsid sequence between amino acids at positions 454, 455, 457, 458, 459, 460, and/or 461 (Loop IV).

In some embodiments, the peptide, e.g., targeting peptide, is inserted into a parent AAV capsid sequence between amino acids at positions 586, 587, 588, 589, and/or 590 (Loop VIII).

In some embodiments, the peptide, e.g., targeting peptide, is inserted into a parent AAV capsid sequence Loop IV. As a non-limiting example, the parent AAV capsid may be AAV5. As a non-limiting example, the parent AAV capsid may be AAV9. As a non-limiting example, the parent AAV capsid may be AAV9hu.14 (SEQ ID NO: 137 or 138). As a non-limiting example, the parent AAV capsid may be AAV9 K449R (SEQ ID NO: 11). As a non-limiting example, the parent AAV capsid may be PHP.B (SEQ ID NO: 5 or 6). As a non-limiting example, the parent AAV capsid may be PHP.N (SEQ ID NO: 4). As a non-limiting example, the parent AAV capsid may be VOY101 (SEQ ID NO: 1 or 2). As a non-limiting example, the parent AAV capsid may be VOY201 (SEQ ID NO: 3 or 1724).

In some embodiments, the peptide, e.g., targeting peptide, are inserted into a parent AAV capsid sequence Loop VIII. As a non-limiting example, the parent AAV capsid may be AAV5. As a non-limiting example, the parent AAV capsid may be AAV9. As a non-limiting example, the parent AAV capsid may be AAV9hu.14 (SEQ ID NO: 137 or 138). As a non-limiting example, the parent AAV capsid may be AAV9 K449R (SEQ ID NO: 11). As a non-limiting example, the parent AAV capsid may be PHP.B (SEQ ID NO: 5 or 6). As a non-limiting example, the parent AAV capsid may be PHP.N (SEQ ID NO: 4). As a non-limiting example, the parent AAV capsid may be VOY101 (SEQ ID NO: 1 or 2). As a non-limiting example, the parent AAV capsid may be VOY201 (SEQ ID NO: 3 or 1724).

In some embodiments, the peptide, e.g., a targeting peptide, is present in loop VIII of an AAV capsid polypeptide, e.g., an AAV capsid variant. In some embodiments, the peptide of an AAV capsid variant described herein, is present immediately subsequent to position 586, relative to a reference sequence numbered according to the amino acid sequence of SEQ ID NO: 138. In some embodiments, the peptide of an AAV capsid variant described herein, is present immediately subsequent to position 588, relative to a reference sequence numbered according to the amino acid sequence of SEQ ID NO: 138. In some embodiments, the peptide of an AAV capsid variant described herein, is present immediately subsequent to position 589, relative to a reference sequence numbered according to the amino acid sequence of SEQ ID NO: 138.

In some embodiments, the peptide, e.g., targeting peptide, of an AAV capsid polypeptide, e.g., an AAV capsid variant described herein comprises the amino acid sequence of PLNGAVHLY (SEQ ID NO: 3648), wherein the amino acid sequence of PLNGAVHLY (SEQ ID NO: 3648) is present immediately subsequent to position 586, relative to a reference sequence numbered according to the amino acid sequence of SEQ ID NO: 138.

In some embodiments, the peptide, e.g., targeting peptide, of an AAV capsid polypeptide, e.g., an AAV capsid variant described herein comprises the amino acid sequence of GGTLAVVSL (SEQ ID NO: 3654), wherein the amino acid sequence of GGTLAVVSL (SEQ ID NO: 3654) is present immediately subsequent to position 586, relative to a reference sequence numbered according to the amino acid sequence of SEQ ID NO: 138.

In some embodiments, the peptide, e.g., targeting peptide, of an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein comprises the amino acid sequence of IVMNSLK (SEQ ID NO: 3651), wherein the amino acid sequence of IVMNSLK (SEQ ID NO: 3651) is present immediately subsequent to position 588, relative to a reference sequence numbered according to the amino acid sequence of SEQ ID NO: 138.

In some embodiments, the peptide, e.g., targeting peptide, of an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein comprises the amino acid sequence of any of SEQ ID NOs: 3649, 3650, 3652, 3653, or 3655-3659, wherein the amino acid sequence of any of the aforesaid sequences is present immediately subsequent to position 589, relative to a reference sequence numbered according to the amino acid sequence of SEQ ID NO: 138.

The peptide, e.g., targeting peptide, described herein may increase the transduction of an AAV particle of the disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) to a target tissue as compared to the parent AAV particle lacking a peptide, e.g., targeting peptide insert. In some embodiments, the peptide, e.g., targeting peptide increases the transduction of an AAV particle to a target tissue by at least about 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 100%, 125%, 150%, 200%, 300%, 400%, 500%, or more as compared to a parent AAV particle lacking a peptide, e.g., targeting peptide, insert.

In some embodiments, the peptide, e.g., targeting peptide, increases the transduction of an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) to a cell, region, or tissue of the CNS (e.g., a brain cell, brain tissue, brain region, spinal cord cell, spinal cord region, or spinal cord tissue) by at least about 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 100%, 125%, 150%, 200%, 300%, 400%, 500%, or more as compared to a parent AAV particle lacking a peptide, e.g., targeting peptide, insert. In some embodiments, the brain region comprises a frontal cortex, sensory cortex, motor cortex, putamen, thalamus, cerebellar cortex, dentate nucleus, caudate, and/or hippocampus. In some embodiments, the spinal cord region comprises a cervical, thoracic, and/or lumbar region.

In some embodiments, the peptide, e.g., targeting peptide, increases the transduction of an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) to a cell or tissue of the PNS by at least about 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 100%, 125%, 150%, 200%, 300%, 400%, 500%, or more as compared to a parent AAV particle lacking a peptide, e.g., targeting peptide, insert.

In some embodiments, the peptide, e.g., targeting peptide, increases the transduction of an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) to a cell or tissue of the DRG by at least about 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 100%, 125%, 150%, 200%, 300%, 400%, 500%, or more as compared to a parent AAV particle lacking a peptide, e.g., targeting peptide, insert.

In some embodiments, the peptide, e.g., targeting peptide, increases the transduction of an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) to a cell, region, or tissue of a muscle by at least about 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 100%, 125%, 150%, 200%, 300%, 400%, 500%, or more as compared to a parent AAV particle lacking a targeting peptide insert. In some embodiments, a muscle region may comprise a heart muscle, quadriceps muscle, and/or a diaphragm muscle region. In some embodiments, the muscle region comprises a heart muscle region, e.g., a heart atrium muscle region or a heart ventricle muscle region,

In some embodiments, an AAV particle described herein comprises an AAV capsid polypeptide, e.g., an AAV capsid polypeptide, e.g., an AAV capsid variant. In some embodiments, the AAV capsid variant comprises a peptide, e.g., targeting peptide, sequence as described in Table 1 or 2.

In some embodiments, the AAV capsid polypeptide, e.g., the AAV capsid variant, comprises at least 3, 4, 5, 6, 7, 8, or 9 consecutive amino acids from the amino acid sequence of any of SEQ ID NO: 1725-3622. In some embodiments, the AAV capsid variant comprises at least 3, 4, 5, 6, 7, 8, or 9 consecutive amino acids from the amino acid sequence of any of SEQ ID NO: 3648-3659. In some embodiments, the amino acid sequence is present in loop VIII. In some embodiments, the amino acid sequence is present immediately subsequent to position 586, 588, or 589, relative to a reference sequence numbered according to the amino acid sequence of SEQ ID NO: 138.

In some embodiments, the 3 consecutive amino acids comprise PLN. In some embodiments, the 4 consecutive amino acids comprise PLNG (SEQ ID NO: 3678). In some embodiments, the 5 consecutive amino acids comprise PLNGA (SEQ ID NO: 3679). In some embodiments, the 6 consecutive amino acids comprise PLNGAV (SEQ ID NO: 3680). In some embodiments, the 7 consecutive amino acids comprise PLNGAVH (SEQ ID NO: 3681). In some embodiments, the 8 consecutive amino acids comprise PLNGAVHL (SEQ ID NO: 3682). In some embodiments, the 9 consecutive amino acids comprise PLNGAVHLY (SEQ ID NO: 3648).

In some embodiments, the 3 consecutive amino acids comprise YST. In some embodiments, the 4 consecutive amino acids comprise YSTD (SEQ ID NO: 3690). In some embodiments, the 5 consecutive amino acids comprise YSTDE (SEQ ID NO: 3691). In some embodiments, the 5 consecutive amino acids comprise YSTDV (SEQ ID NO: 3700). In some embodiments, the 6 consecutive amino acids comprise YSTDER (SEQ ID NO: 3692). In some embodiments, the 6 consecutive amino acids comprise YSTDVR (SEQ ID NO: 3701). In some embodiments, the 7 consecutive amino acids comprise YSTDERM (SEQ ID NO: 3657). In some embodiments, the 7 consecutive amino acids comprise YSTDERK (SEQ ID NO: 3658). In some embodiments, the 7 consecutive amino acids comprise YSTDVRM (SEQ ID NO: 3650).

In some embodiments, the 3 consecutive amino acids comprise IVM. In some embodiments, the 4 consecutive amino acids comprise IVMN (SEQ ID NO: 3693). In some embodiments, the 5 consecutive amino acids comprise IVMNS (SEQ ID NO: 3694). In some embodiments, the 6 consecutive amino acids comprise IVMNSL (SEQ ID NO: 3695). In some embodiments, the 7 consecutive amino acids comprise IVMNSLK (SEQ ID NO: 3651).

In some embodiments, the AAV capsid polypeptide, e.g., the AAV capsid variant, comprises an amino acid sequence comprising at least one, two, or three but no more than four modifications, e.g., substitutions, relative to the amino acid sequence of any of SEQ ID NO: 1725-3622. In some embodiments, the AAV capsid variant comprises an amino acid sequence comprising at least one, two, or three but no more than four modifications, e.g., substitutions, relative to the amino acid sequence of any of SEQ ID NO: 3648-3659. In some embodiments, the amino acid sequence is present in loop VIII. In some embodiments, the amino acid sequence is present immediately subsequent to position 586, 588, or 589, relative to a reference sequence numbered according to the amino acid sequence of SEQ ID NO: 138.

In some embodiments, the AAV capsid polypeptide, e.g., the AAV capsid variant, comprises the amino acid sequence of PLNGAVHLY (SEQ ID NO: 3648), or an amino acid sequence having at least one, two, or three but no more than four modifications, e.g., substitutions, relative to the amino acid sequence of PLNGAVHLY (SEQ ID NO: 3648), optionally wherein position 7 is H.

In some embodiments, the AAV capsid polypeptide, e.g., the AAV capsid variant, comprises the amino acid sequence of RDSPKGW (SEQ ID NO: 3649), or an amino acid sequence having at least one, two, or three modifications but no more than four modifications, e.g., substitutions, relative to the amino acid sequence of RDSPKGW (SEQ ID NO: 3649).

In some embodiments, the AAV capsid polypeptide, e.g. the AAV capsid variant, comprises the amino acid sequence of IVMNSLK (SEQ ID NO: 3651), or an amino acid sequence having at least one, two, or three modifications but no more than four modifications, e.g., substitutions, relative to the amino acid sequence of IVMNSLK (SEQ ID NO: 3651).

In some embodiments, the AAV capsid polypeptide, e.g. the AAV capsid variant, comprises the amino acid sequence of YSTDVRM (SEQ ID NO: 3650), or an amino acid sequence having at least one, two, or three modifications but no more than four modifications, e.g., substitutions, relative to the amino acid sequence of YSTDVRM (SEQ ID NO: 3650).

In some embodiments, the AAV capsid polypeptide, e.g., the AAV capsid variant, comprises the amino acid sequence of RESPRGL (SEQ ID NO: 3652), or a sequence having at least one, two, or three modifications but no more than four modifications, e.g., substitutions, relative to the amino acid sequence of RESPRGL (SEQ ID NO: 3652).

In some embodiments, the AAV capsid polypeptide, e.g., the AAV capsid variant, comprises the amino acid sequence of any of SEQ ID NO: 1725-3622. In some embodiments, the AAV capsid variant comprises the amino acid sequence of any of SEQ ID NO: 3648-3659. In some embodiments, the amino acid sequence is present in loop VIII of an AAV capsid variant described herein. In some embodiments, the amino acid sequence is present immediately subsequent to position 586, relative to a reference sequence numbered according to the amino acid sequence of SEQ ID NO: 138. In some embodiments, the amino acid sequence is present immediately subsequent to position 588, relative to a reference sequence numbered according to the amino acid sequence of SEQ ID NO: 138. In some embodiments, the amino acid sequence is present immediately subsequent to position 589, relative to a reference sequence numbered according to the amino acid sequence of SEQ ID NO: 138.

In some embodiments, the AAV capsid polypeptide, e.g., the AAV capsid variant (e.g., an AAV capsid variant described herein), comprises an amino acid sequence encoded by the nucleotide sequence of any one of SEQ ID NOs: 3660-3671, or a nucleotide sequence substantially identical (e.g., having at least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence identity) thereto. In some embodiments, the AAV capsid, e.g., an AAV capsid variant described herein, comprises an amino acid sequence encoded by a nucleotide sequence comprising at least one, two, three, four, five, six, or seven modifications but no more than ten modifications of the nucleotide sequences of any of SEQ ID NOs: 3660-3671.

In some embodiments, the nucleotide sequence encoding the AAV capsid polypeptide, e.g., the AAV capsid variant (e.g., an AAV capsid variant described herein), comprises the nucleotide sequence of any one of SEQ ID NOs: 3660-3671, or a nucleotide sequence substantially identical (e.g., having at least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence identity) thereto. In some embodiments, nucleic acid sequence encoding the AAV capsid variant, e.g., an AAV capsid variant described herein, comprises a nucleotide sequence comprising at least one, two, three, four, five, six, or seven modifications but no more than ten modifications of the nucleotide sequences of any of SEQ ID NOs: 3660-3671.

In some embodiments, the nucleotide sequence encoding the AAV capsid polypeptide, e.g., the AAV capsid variant (e.g., an AAV capsid variant described herein), comprises the nucleotide sequence of SEQ ID NO: 3660, or a nucleotide sequence substantially identical (e.g., having at least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence identity) thereto. In some embodiments, the nucleic acid sequence encoding the AAV capsid variant comprises a nucleotide sequence comprising at least one, two, three, four, five, six, or seven modifications but no more than ten modifications of the nucleotide sequences of SEQ ID NO: 3660.

In some embodiments, the nucleotide sequence encoding the AAV capsid polypeptide, e.g., the AAV capsid variant (e.g., an AAV capsid variant described herein), comprises the nucleotide sequence of SEQ ID NO: 3663, or a nucleotide sequence substantially identical (e.g., having at least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence identity) thereto. In some embodiments, the nucleic acid sequence encoding the AAV capsid variant comprises a nucleotide sequence comprising at least one, two, three, four, five, six, or seven modifications but no more than ten modifications of the nucleotide sequences of SEQ ID NO: 3663.

In some embodiments, the AAV capsid polypeptide, e.g., the AAV capsid variant, comprises an amino acid residue other than “A” at position 587 and/or an amino acid residue other than “Q” at position 588, numbered according to SEQ ID NO: 138.

In some embodiments, the AAV capsid polypeptide, e.g., the AAV capsid variant, comprises the amino acid sequence of PLNGAVHLY (SEQ ID NO: 3648) wherein the amino acid sequence of PLNGAVHLY (SEQ ID NO: 3648) is present immediately subsequent to position 586, relative to a reference sequence numbered according to the amino acid sequence of SEQ ID NO: 138.

In some embodiments, the polypeptide, e.g., the AAV capsid variant, comprises the amino acid sequence of GGTLAVVSL (SEQ ID NO: 3654), wherein the amino acid sequence of GGTLAVVSL (SEQ ID NO: 3654) is present immediately subsequent to position 586, relative to a reference sequence numbered according to the amino acid sequence of SEQ ID NO: 138.

In some embodiments, the AAV capsid polypeptide, e.g., the AAV capsid variant, comprises the amino acid sequence of IVMNSLK (SEQ ID NO: 3651), wherein the amino acid sequence of IVMNSLK (SEQ ID NO: 3651) is present immediately subsequent to position 588, relative to a reference sequence numbered according to the amino acid sequence of SEQ ID NO: 138.

In some embodiments, the AAV capsid polypeptide, e.g., the AAV capsid variant, comprises the amino acid sequence of any of SEQ ID NOs: 3649, 3650, 3652, 3653, or 3655-3659, wherein the amino acid sequence of any of the aforesaid sequences is present immediately subsequent to position 589, relative to a reference sequence numbered according to the amino acid sequence of SEQ ID NO: 138.

In some embodiments, the AAV capsid polypeptide, e.g., the AAV capsid variant, further comprises a substitution at position K449, e.g., a K449R substitution, numbered according to SEQ ID NO: 138. In some embodiments, the AAV capsid variant further comprises a modification, e.g., an insertion, substitution, and/or deletion in loop I, II, IV, and/or VI.

In some embodiments, the AAV capsid polypeptide, e.g., the AAV capsid variant, further comprises an amino acid sequence having at least one, two or three modifications but not more than 30, 20 or 10 modifications of the amino acid sequence of SEQ ID NO: 138. In some embodiments, the AAV capsid variant further comprises the amino acid sequence of SEQ ID NO: 138, or an amino acid sequence with at least 80% (e.g., at least about 85, 90, 95, 96, 97, 98, or 99%) sequence identity thereto. In some embodiments, the AAV capsid variant further comprises an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 137, or a sequence with at least 80% (e.g., at least about 85, 90, 95, 96, 97, 98, or 99%) sequence identity thereto.

In some embodiments, an AAV capsid polypeptide, e.g., an AAV capsid variant, of the present disclosure comprises a parental amino acid sequence having an insert, e.g., a peptide, e.g., a targeting peptide, wherein the insert comprises the amino acid sequence of any of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the insert comprises at least 3, 4, 5, 6, 7, 8, or 9 consecutive amino acids from the amino acid sequence of any of SEQ ID NO: 1725-3622 or 3648-3659. In some embodiments, the insert comprises an amino acid sequence comprising at least one, two, or three modifications, but no more than four modifications, e.g., substitutions, relative to the amino acid sequence of any of SEQ ID NO: 1725-3622 or 3648-3659.

In some embodiments, the parental sequence of an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein comprises an amino acid sequence comprising at least one, two, or three modifications but no more than 30, 20 or 10 modifications, e.g., substitutions, to the amino acid sequence of SEQ ID NO: 138. In some embodiments, the parental sequence comprises the amino acid sequence of SEQ ID NO: 138, or an amino acid sequence substantially identical (e.g., having at least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence identity) thereto. In some embodiments, the parental sequence further comprises a substitution at position K449, e.g., a K449R substitution. In some embodiments, the parental sequence comprises an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 137, or a nucleotide sequence substantially identical (e.g., having at least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence identity) thereto. In some embodiments, the polynucleotide encoding the parental sequence described herein comprises the nucleotide sequence of SEQ ID NO: 137, or a nucleotide sequence substantially identical (e.g., having at least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence identity) thereto.

In some embodiments, the parental sequence of an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein comprises an amino acid sequence comprising at least one, two, or three modifications but no more than 30, 20 or 10 modifications, e.g., substitutions, to the amino acid sequence of SEQ ID NO: 11, e.g., provided that position 449 of SEQ ID NO: 11 is not K, e.g., is R. In some embodiments, the parental sequence of an AAV capsid variant described herein comprises the amino acid sequence of SEQ ID NO: 11 or an amino acid sequence substantially identical (e.g., having at least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence identity) thereto, e.g., provided that position 449 of SEQ ID NO: 11 is not K, e.g., is R.

In some embodiments, the insert of an AAV capsid variant is inserted into loop VIII of the parental amino acid sequence described herein. In some embodiments, the insert is inserted immediately subsequent to position 586, 588, or 589 in the parental amino acid sequence described herein.

In some embodiments, the AAV capsid variant further comprises an amino acid other than “A” at position 587 and/or an amino acid other than “Q” at position 588 of the parental sequence described herein. In some embodiments, the AAV capsid variant further comprises a deletion at position 587 and/or a deletion at position 588 of the parental amino acid sequence described herein. In some embodiments, the AAV capsid variant comprises a deletion of the amino acids “AQ” at positions 587-588 of the parental amino acid sequence described herein.

In some embodiment, the insert of an AAV capsid variant described herein comprises the amino acid sequence of PLNGAVHLY (SEQ ID NO: 3648). In some embodiments, the insert sequence of PLNGAVHLY (SEQ ID NO: 3648) is inserted immediately subsequent to position 586 of the parental amino acid sequence. In some embodiments, the AAV capsid variant further comprises a deletion of the amino acids “AQ” at positions 587-588 of the parental amino acid sequence. In some embodiments, the AAV capsid variant comprises an insert comprising the amino acid sequence of PLNGAVHLY (SEQ ID NO: 3648), which is inserted immediately subsequent to position 586 of the parental amino acid sequence and a deletion of the amino acids “AQ” at positions 587-588 of the parental amino acid sequence.

In some embodiments, the insert of an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein comprises the amino acid sequence of GGTLAVVSL (SEQ ID NO: 3654). In some embodiments, the insert sequence of GGTLAVVSL (SEQ ID NO: 3654) is inserted immediately subsequent to position 586 of the parental amino acid sequence. In some embodiments, the AAV capsid variant further comprises a deletion of the amino acids “AQ” at positions 587-588 of the parental amino acid sequence. In some embodiments, the AAV capsid variant comprises an insert comprising the amino acid sequence of GGTLAVVSL (SEQ ID NO: 3654), which is inserted immediately subsequent to position 586 of the parental amino acid sequence and a deletion of the amino acids “AQ” at positions 587-588 of the parental amino acid sequence.

In some embodiments, the insert of an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein comprises the amino acid sequence of IVMNSLK (SEQ ID NO: 3651). In some embodiments, the insert sequence of IVMNSLK (SEQ ID NO: 3651) is inserted immediately subsequent to position 588 of the parental amino acid sequence. In some embodiments, the AAV capsid variant comprises an insert comprising the amino acid sequence of IVMNSLK (SEQ ID NO: 3651), which is inserted immediately subsequent to position 589 of the parental amino acid sequence.

In some embodiments, the insert of an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein comprises the amino acid sequence of RDSPKGW (SEQ ID NO: 3649), YSTDVRM (SEQ ID NO: 3650), RESPRGL (SEQ ID NO: 3652), SFNDTRA (SEQ ID NO: 3653), YGLPKGP (SEQ ID NO: 3655) or STGTLRL (SEQ ID NO: 3656). In some embodiments, the insert sequence of RDSPKGW (SEQ ID NO: 3649), YSTDVRM (SEQ ID NO: 3650), RESPRGL (SEQ ID NO: 3652), SFNDTRA (SEQ ID NO: 3653), YGLPKGP (SEQ ID NO: 3655) or STGTLRL (SEQ ID NO: 3656) is inserted immediately subsequent to position 589 of the parental amino acid sequence. In some embodiments, the AAV capsid variant comprises an insert comprising the amino acid sequence of RDSPKGW (SEQ ID NO: 3649), YSTDVRM (SEQ ID NO: 3650), RESPRGL (SEQ ID NO: 3652), SFNDTRA (SEQ ID NO: 3653), YGLPKGP (SEQ ID NO: 3655) or STGTLRL (SEQ ID NO: 3656), which is inserted immediately subsequent to position 589 of the parental amino acid sequence.

In some embodiments, an AAV capsid polypeptide, e.g., an AAV capsid variant, of the present disclosure comprises an amino acid sequence as described herein, e.g. an amino acid sequence of an AAV capsid variant chosen from TTD-001, TTD-002, TTD-003, TTD-004, TTD-005, TTD-006, TTD-007, TTD-008, TTD-009, TTD-010, TTD-011, or TTD-012, e.g., as described in Tables 3 and 4.

In some embodiments, an AAV capsid polypeptide, e.g. the AAV capsid variant, comprises a VP1, VP2, and/or VP3 protein comprising an amino acid sequence described herein, e.g. an amino acid sequence of an AAV capsid variant chosen from TTD-001, TTD-002, TTD-003, TTD-004, TTD-005, TTD-006, TTD-007, TTD-008, TTD-009, TTD-010, TTD-011, or TTD-012, e.g., as described in Tables 3 and 4.

In some embodiments, an AAV capsid polypeptide, e.g., the AAV capsid variant, comprises an amino acid sequence encoded by a nucleotide sequence as described herein, e.g. a nucleotide sequence of an AAV capsid variant chosen from TTD-001, TTD-002, TTD-003, TTD-004, TTD-005, TTD-006, TTD-007, TTD-008, TTD-009, TTD-010, TTD-011, or TTD-012, e.g., as described in Tables 3 and 5.

In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, of the present disclosure comprises a nucleotide sequence described herein, e.g. a nucleotide sequence of an AAV capsid variant chosen from TTD-001, TTD-002, TTD-003, TTD-004, TTD-005, TTD-006, TTD-007, TTD-008, TTD-009, TTD-010, TTD-011, or TTD-012, e.g., as described in Tables 3 and 5.

In some embodiments, insertion of a nucleic acid sequence, targeting nucleic acid sequence, or a peptide, e.g., targeting peptide, into a parent AAV sequence generates the non-limiting exemplary full length capsid sequences, e.g., an AAV capsid polypeptide, e.g., an AAV capsid variant, as described in Tables 3, 4 and 5.

TABLE 3 Exemplary full length capsid sequences (VP1 with insert) VP1 VP1 Peptide DNA PRT Peptide DNA SEQ ID SEQ PRT SEQ SEQ Serotype NO: ID NO: ID NO: ID NO: TTD-001 3623 3636 1725 or 3648 3660 TTD-002 3624 or 3637 1726 or 3649 3661 3625 TTD-003 3626 3638 1729 or 3650 3662 TTD-004 3627 3639 1760 or 3651 3663 TTD-005 3628 3640 1769 or 3652 3664 TTD-006 3629 3641 3622 or 3653 3665 TTD-007 3630 3642 1798 or 3654 3666 TTD-008 3631 3643 1785 or 3655 3667 TTD-009 3632 3644 1767 or 3656 3668 TTD-010 3633 3645 1734 or 3657 3669 TTD-011 3634 3646 1737 or 3658 3670 TTD-012 3635 3647 1819 or 3659 3671

TABLE 4 Exemplary full length capsid amino acid sequences SEQ Name and ID Annotation NO: Amino Acid Sequence TTD-001 3636 MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPG 9 mer peptide YKYLGPGNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADA underlined, starts at EFQERLKEDTSFGGNLGRAVFQAKKRLLEPLGLVEEAAKTAPGKKRPVE position 587 QSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPS (immediately GVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVITTSTR subsequent to TWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFS position 586); PRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTSTVQ 743 aa VFTDSDYQLPYVLGSAHEGCLPPFPADVFMIPQYGYLTLNDGSQAVGRS SFYCLEYFPSQMLRTGNNFQFSYEFENVPFHSSYAHSQSLDRLMNPLID QYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNYIPGPSYRQQRVS TTVTQNNNSEFAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSG SLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATESYGQVATNHQS PL NGAVHLY AQAQTGWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFH PSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQV SVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEFAVNTEGVYSEPRPIG TRYLTRNL TTD-002 3637 MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPG 7 mer peptide YKYLGPGNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADA underlined, starts at EFQERLKEDTSFGGNLGRAVFQAKKRLLEPLGLVEEAAKTAPGKKRPVE position 590 QSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPS (immediately GVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVITTSTR subsequent to TWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFS position 589); PRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTSTVQ 743 aa VFTDSDYQLPYVLGSAHEGCLPPFPADVFMIPQYGYLTLNDGSQAVGRS SFYCLEYFPSQMLRTGNNFQFSYEFENVPFHSSYAHSQSLDRLMNPLID QYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNYIPGPSYRQQRVS TTVTQNNNSEFAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSG SLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATESYGQVATNHQSAQ A RDSPKGW QAQTGWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFH PSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQV SVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEFAVNTEGVYSEPRPIG TRYLTRNL TTD-003 3638 MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPG 7 mer peptide YKYLGPGNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADA underlined, starts at EFQERLKEDTSFGGNLGRAVFQAKKRLLEPLGLVEEAAKTAPGKKRPVE position 590 QSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPS (immediately GVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVITTSTR subsequent to TWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFS position 589); PRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTSTVQ 743 aa VFTDSDYQLPYVLGSAHEGCLPPFPADVFMIPQYGYLTLNDGSQAVGRS SFYCLEYFPSQMLRTGNNFQFSYEFENVPFHSSYAHSQSLDRLMNPLID QYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNYIPGPSYRQQRVS TTVTQNNNSEFAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSG SLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATESYGQVATNHQSAQ A YSTDVRM QAQTGWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFH PSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQV SVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEFAVNTEGVYSEPRPIG TRYLTRNL TTD-004 3639 MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPG 7 mer peptide YKYLGPGNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADA underlined, starts at EFQERLKEDTSFGGNLGRAVFQAKKRLLEPLGLVEEAAKTAPGKKRPVE position 589 QSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPS (immediately GVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVITTSTR subsequent to TWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFS position 588); PRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTSTVQ 743 aa VFTDSDYQLPYVLGSAHEGCLPPFPADVFMIPQYGYLTLNDGSQAVGRS SFYCLEYFPSQMLRTGNNFQFSYEFENVPFHSSYAHSQSLDRLMNPLID QYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNYIPGPSYRQQRVS TTVTQNNNSEFAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSG SLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATESYGQVATNHQSAQ IVMNSLK AQAQTGWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFH PSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQV SVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEFAVNTEGVYSEPRPIG TRYLTRNL TTD-005 3640 MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPG 7 mer peptide YKYLGPGNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADA underlined, starts at EFQERLKEDTSFGGNLGRAVFQAKKRLLEPLGLVEEAAKTAPGKKRPVE position 590 QSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPS (immediately GVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVITTSTR subsequent to TWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFS position 589); PRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTSTVQ 743 aa VFTDSDYQLPYVLGSAHEGCLPPFPADVFMIPQYGYLTLNDGSQAVGRS SFYCLEYFPSQMLRTGNNFQFSYEFENVPFHSSYAHSQSLDRLMNPLID QYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNYIPGPSYRQQRVS TTVTQNNNSEFAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSG SLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATESYGQVATNHQSAQ A RESPRGL QAQTGWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFH PSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQV SVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEFAVNTEGVYSEPRPIG TRYLTRNL TTD-006 3641 MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPG 7 mer peptide YKYLGPGNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADA underlined, starts at EFQERLKEDTSFGGNLGRAVFQAKKRLLEPLGLVEEAAKTAPGKKRPVE position 590 QSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPS (immediately GVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVITTSTR subsequent to 589); TWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFS 743 aa PRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTSTVQ VFTDSDYQLPYVLGSAHEGCLPPFPADVFMIPQYGYLTLNDGSQAVGRS SFYCLEYFPSQMLRTGNNFQFSYEFENVPFHSSYAHSQSLDRLMNPLID QYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNYIPGPSYRQQRVS TTVTQNNNSEFAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSG SLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATESYGQVATNHQSAQ A SFNDTRA QAQTGWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFH PSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQV SVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEFAVNTEGVYSEPRPIG TRYLTRNL TTD-007 3642 MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPG 9 mer peptide YKYLGPGNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADA underlined, starts at EFQERLKEDTSFGGNLGRAVFQAKKRLLEPLGLVEEAAKTAPGKKRPVE position 587 QSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPS (immediately GVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVITTSTR subsequent to TWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFS position 586); PRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTSTVQ 743 aa VFTDSDYQLPYVLGSAHEGCLPPFPADVFMIPQYGYLTLNDGSQAVGRS SFYCLEYFPSQMLRTGNNFQFSYEFENVPFHSSYAHSQSLDRLMNPLID QYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNYIPGPSYRQQRVS TTVTQNNNSEFAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSG SLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATESYGQVATNHQS GG TLAVVSL AQAQTGWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFH PSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQV SVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEFAVNTEGVYSEPRPIG TRYLTRNL TTD-008 3643 MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPG 7 mer peptide YKYLGPGNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADA underlined, starts at EFQERLKEDTSFGGNLGRAVFQAKKRLLEPLGLVEEAAKTAPGKKRPVE position 590 QSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPS (immediately GVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVITTSTR subsequent to 589); TWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFS 743 aa PRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTSTVQ VFTDSDYQLPYVLGSAHEGCLPPFPADVFMIPQYGYLTLNDGSQAVGRS SFYCLEYFPSQMLRTGNNFQFSYEFENVPFHSSYAHSQSLDRLMNPLID QYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNYIPGPSYRQQRVS TTVTQNNNSEFAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSG SLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATESYGQVATNHQSAQ A YGLPKGP QAQTGWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFH PSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQV SVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEFAVNTEGVYSEPRPIG TRYLTRNL TTD-009 3644 MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPG 7 mer peptide YKYLGPGNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADA underlined, starts at EFQERLKEDTSFGGNLGRAVFQAKKRLLEPLGLVEEAAKTAPGKKRPVE position 590 QSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPS (immediately GVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVITTSTR subsequent to 589); TWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFS 743 aa PRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTSTVQ VFTDSDYQLPYVLGSAHEGCLPPFPADVFMIPQYGYLTLNDGSQAVGRS SFYCLEYFPSQMLRTGNNFQFSYEFENVPFHSSYAHSQSLDRLMNPLID QYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNYIPGPSYRQQRVS TTVTQNNNSEFAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSG SLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATESYGQVATNHQSAQ A STGTLRL QAQTGWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFH PSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQV SVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEFAVNTEGVYSEPRPIG TRYLTRNL TTD-010 3645 MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPG 7 mer peptide YKYLGPGNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADA underlined, starts at EFQERLKEDTSFGGNLGRAVFQAKKRLLEPLGLVEEAAKTAPGKKRPVE position 590 QSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPS (immediately GVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVITTSTR subsequent to 589); TWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFS 743 aa PRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTSTVQ VFTDSDYQLPYVLGSAHEGCLPPFPADVFMIPQYGYLTLNDGSQAVGRS SFYCLEYFPSQMLRTGNNFQFSYEFENVPFHSSYAHSQSLDRLMNPLID QYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNYIPGPSYRQQRVS TTVTQNNNSEFAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSG SLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATESYGQVATNHQSAQ A YSTDERM QAQTGWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFH PSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQV SVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEFAVNTEGVYSEPRPIG TRYLTRNL TTD-011 3646 MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPG 7 mer peptide YKYLGPGNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADA underlined, starts at EFQERLKEDTSFGGNLGRAVFQAKKRLLEPLGLVEEAAKTAPGKKRPVE position 590 QSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPS (immediately GVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVITTSTR subsequent to 589); TWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFS 743 aa PRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTSTVQ VFTDSDYQLPYVLGSAHEGCLPPFPADVFMIPQYGYLTLNDGSQAVGRS SFYCLEYFPSQMLRTGNNFQFSYEFENVPFHSSYAHSQSLDRLMNPLID QYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNYIPGPSYRQQRVS TTVTQNNNSEFAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSG SLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATESYGQVATNHQSAQ A YSTDERK QAQTGWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFH PSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQV SVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEFAVNTEGVYSEPRPIG TRYLTRNL TTD-012 3647 MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPG 7 mer peptide YKYLGPGNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADA underlined, starts at EFQERLKEDTSFGGNLGRAVFQAKKRLLEPLGLVEEAAKTAPGKKRPVE position 590 QSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPS (immediately GVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVITTSTR subsequent to 589); TWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFS 743 aa PRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTSTVQ VFTDSDYQLPYVLGSAHEGCLPPFPADVFMIPQYGYLTLNDGSQAVGRS SFYCLEYFPSQMLRTGNNFQFSYEFENVPFHSSYAHSQSLDRLMNPLID QYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNYIPGPSYRQQRVS TTVTQNNNSEFAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSG SLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATESYGQVATNHQSAQ A YVSSVKM QAQTGWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFH PSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQV SVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEFAVNTEGVYSEPRPIG TRYLTRNL

TABLE 5 Exemplary full length capsid nucleic acid sequences SEQ Name and ID Annotation NO: NT Sequence TTD-001 3623 atggctgccgatggttatcttccagattggctcgaggacaaccttagtgaaggaattcgcg 9mer peptide agtggtgggctttgaaacctggagcccctcaacccaaggcaaatcaacaacatcaagacaa underlined cgctcgaggtcttgtgcttccgggttacaaataccttggacccggcaacggactcgacaag ggggagccggtcaacgcagcagacgcggcggccctcgagcacgacaaggcctacgaccagc agctcaaggccggagacaacccgtacctcaagtacaaccacgccgacgccgagttccagga gcggctcaaagaagatacgtcttttgggggcaacctcgggcgagcagtcttccaggccaaa aagaggcttcttgaacctcttggtctggttgaggaagcggctaagacggctcctggaaaga agaggcctgtagagcagtctcctcaggaaccggactcctccgcgggtattggcaaatcggg tgcacagcccgctaaaaagagactcaatttcggtcagactggcgacacagagtcagtccca gaccctcaaccaatcggagaacctcccgcagccccctcaggtgtgggatctcttacaatgg cttcaggtggtggcgcaccagtggcagacaataacgaaggtgccgatggagtgggtagttc ctcgggaaattggcattgcgattcccaatggctgggggacagagtcatcaccaccagcacc cgaacctgggccctgcccacctacaacaatcacctctacaagcaaatctccaacagcacat ctggaggatcttcaaatgacaacgcctacttcggctacagcaccccctgggggtattttga cttcaacagattccactgccacttctcaccacgtgactggcagcgactcatcaacaacaac tggggattccggcctaagcgactcaacttcaagctcttcaacattcaggtcaaagaggtta cggacaacaatggagtcaagaccatcgccaataaccttaccagcacggtccaggtcttcac ggactcagactatcagctcccgtacgtgctcgggtcggctcacgagggctgcctcccgccg ttcccagcggacgttttcatgattcctcagtacgggtatctgacgcttaatgatggaagcc aggccgtgggtcgttcgtccttttactgcctggaatatttcccgtcgcaaatgctaagaac gggtaacaacttccagttcagctacgagtttgagaacgtacctttccatagcagctacgct cacagccaaagcctggaccgactaatgaatccactcatcgaccaatacttgtactatctct caaagactattaacggttctggacagaatcaacaaacgctaaaattcagtgtggccggacc cagcaacatggctgtccagggaagaaactacatacctggacccagctaccgacaacaacgt gtctcaaccactgtgactcaaaacaacaacagcgaatttgcttggcctggagcttcttctt gggctctcaatggacgtaatagcttgatgaatcctggacctgctatggccagccacaaaga aggagaggaccgtttctttcctttgtctggatctttaatttttggcaaacaaggaactgga agagacaacgtggatgcggacaaagtcatgataaccaacgaagaagaaattaaaactacta acccggtagcaacggagtcctatggacaagtggccacaaaccaccagagt ccgcttaatgg tgccgtccatctttat gctcaggcgcagaccggctgggttcaaaaccaaggaatacttccg ggtatggtttggcaggacagagatgtgtacctgcaaggacccatttgggccaaaattcctc acacggacggcaactttcacccttctccgctgatgggagggtttggaatgaagcacccgcc tcctcagatcctcatcaaaaacacacctgtacctgcCgatcctccaacggccttcaacaag gacaagctgaactctttcatcacccagtattctactggccaagtcagcgtggagatcgagt gggagctgcagaaggaaaacagcaagcgOtggaacccggagatccagtacacttccaacta ttacaagtctaataatgttgaatttgctgttaatactgaaggtgtatatagtgaaccccgc cccattggcaccagatacctgactcgtaatctgtaa TTD-002 3624 atggctgccgatggttatcttccagattggctcgaggacaaccttagtgaaggaattcgcg 7mer peptide agtggtgggctttgaaacctggagcccctcaacccaaggcaaatcaacaacatcaagacaa underlined cgctcgaggtcttgtgcttccgggttacaaataccttggacccggcaacggactcgacaag ggggagccggtcaacgcagcagacgcggcggccctcgagcacgacaaggcctacgaccagc agctcaaggccggagacaacccgtacctcaagtacaaccacgccgacgccgagttccagga gcggctcaaagaagatacgtcttttgggggcaacctcgggcgagcagtcttccaggccaaa aagaggcttcttgaacctcttggtctggttgaggaagcggctaagacggctcctggaaaga agaggcctgtagagcagtctcctcaggaaccggactcctccgcgggtattggcaaatcggg tgcacagcccgctaaaaagagactcaatttcggtcagactggcgacacagagtcagtccca gaccctcaaccaatcggagaacctcccgcagccccctcaggtgtgggatctcttacaatgg cttcaggtggtggcgcaccagtggcagacaataacgaaggtgccgatggagtgggtagttc ctcgggaaattggcattgcgattcccaatggctgggggacagagtcatcaccaccagcacc cgaacctgggccctgcccacctacaacaatcacctctacaagcaaatctccaacagcacat ctggaggatcttcaaatgacaacgcctacttcggctacagcaccccctgggggtattttga cttcaacagattccactgccacttctcaccacgtgactggcagcgactcatcaacaacaac tggggattccggcctaagcgactcaacttcaagctcttcaacattcaggtcaaagaggtta cggacaacaatggagtcaagaccatcgccaataaccttaccagcacggtccaggtcttcac ggactcagactatcagctcccgtacgtgctcgggtcggctcacgagggctgcctcccgccg ttcccagcggacgttttcatgattcctcagtacgggtatctgacgcttaatgatggaagcc aggccgtgggtcgttcgtccttttactgcctggaatatttcccgtcgcaaatgctaagaac gggtaacaacttccagttcagctacgagtttgagaacgtacctttccatagcagctacgct cacagccaaagcctggaccgactaatgaatccactcatcgaccaatacttgtactatctct caaagactattaacggttctggacagaatcaacaaacgctaaaattcagtgtggccggacc cagcaacatggctgtccagggaagaaactacatacctggacccagctaccgacaacaacgt gtctcaaccactgtgactcaaaacaacaacagcgaatttgcttggcctggagcttcttctt gggctctcaatggacgtaatagcttgatgaatcctggacctgctatggccagccacaaaga aggagaggaccgtttctttcctttgtctggatctttaatttttggcaaacaaggaactgga agagacaacgtggatgcggacaaagtcatgataaccaacgaagaagaaattaaaactacta acccggtagcaacggagtcctatggacaagtggccacaaaccaccagagtgcacaggct cg tgattctccgaagggttggca ggcgcagaccggctgggttcaaaaccaaggaatacttccg ggtatggtttggcaggacagagatgtgtacctgcaaggacccatttgggccaaaattcctc acacggacggcaactttcacccttctccgctgatgggagggtttggaatgaagcacccgcc tcctcagatcctcatcaaaaacacacctgtacctgcCgatcctccaacggccttcaacaag gacaagctgaactctttcatcacccagtattctactggccaagtcagcgtggagatcgagt gggagctgcagaaggaaaacagcaagcgOtggaacccggagatccagtacacttccaacta ttacaagtctaataatgttgaatttgctgttaatactgaaggtgtatatagtgaaccccgc cccattggcaccagatacctgactcgtaatctgtaa 3625 atggctgccgatggttatcttccagattggctcgaggacaaccttagtgaaggaattcgcg agtggtgggctttgaaacctggagcccctcaacccaaggcaaatcaacaacatcaagacaa cgctcgaggtcttgtgcttccgggttacaaataccttggacccggcaacggactcgacaag ggggagccggtcaacgcagcagacgcggcggccctcgagcacgacaaggcctacgaccagc agctcaaggccggagacaacccgtacctcaagtacaaccacgccgacgccgagttccagga gcggctcaaagaagatacgtcttttgggggcaacctcgggcgagcagtcttccaggccaaa aagaggcttcttgaacctcttggtctggttgaggaagcggctaagacggctcctggaaaga agaggcctgtagagcagtctcctcaggaaccggactcctccgcgggtattggcaaatcggg tgcacagcccgctaaaaagagactcaatttcggtcagactggcgacacagagtcagtccca gaccctcaaccaatcggagaacctcccgcagccccctcaggtgtgggatctcttacaatgg cttcaggtggtggcgcaccagtggcagacaataacgaaggtgccgatggagtgggtagttc ctcgggaaattggcattgcgattcccaatggctgggggacagagtcatcaccaccagcacc cgaacctgggccctgcccacctacaacaatcacctctacaagcaaatctccaacagcacat ctggaggatcttcaaatgacaacgcctacttcggctacagcaccccctgggggtattttga cttcaacagattccactgccacttctcaccacgtgactggcagcgactcatcaacaacaac tggggattccggcctaagcgactcaacttcaagctcttcaacattcaggtcaaagaggtta cggacaacaatggagtcaagaccatcgccaataaccttaccagcacggtccaggtcttcac ggactcagactatcagctcccgtacgtgctcgggtcggctcacgagggctgcctcccgccg ttcccagcggacgttttcatgattcctcagtacgggtatctgacgcttaatgatggaagcc aggccgtgggtcgttcgtccttttactgcctggaatatttcccgtcgcaaatgctaagaac gggtaacaacttccagttcagctacgagtttgagaacgtacctttccatagcagctacgct cacagccaaagcctggaccgactaatgaatccactcatcgaccaatacttgtactatctct caaagactattaacggttctggacagaatcaacaaacgctaaaattcagtgtggccggacc cagcaacatggctgtccagggaagaaactacatacctggacccagctaccgacaacaacgt gtctcaaccactgtgactcaaaacaacaacagcgaatttgcttggcctggagcttcttctt gggctctcaatggacgtaatagcttgatgaatcctggacctgctatggccagccacaaaga aggagaggaccgtttctttcctttgtctggatctttaatttttggcaaacaaggaactgga agagacaacgtggatgcggacaaagtcatgataaccaacgaagaagaaattaaaactacta acccggtagcaacggagtcctatggacaagtggccacaaaccaccagagtgcacaggct cg tgattctccgaagggttggca ggcgcagaccggctgggttcaaaaccaaggaatacttccg ggtatggtttggcaggacagagatgtgtacctgcaaggacccatttgggccaaaattcctc acacggacggcaactttcacccttctccgctgatgggagggtttggaatgaagcacccgcc tcctcagatcctcatcaaaaacacacctgtacctgcggatcctccaacggccttcaacaag gacaagctgaactctttcatcacccagtattctactggccaagtcagcgtggagatcgagt gggagctgcagaaggaaaacagcaagcgctggaacccggagatccagtacacttccaacta ttacaagtctaataatgttgaatttgctgttaatactgaaggtgtatatagtgaaccccgc cccattggcaccagatacctgactcgtaatctgtaa TTD-003 3626 atggctgccgatggttatcttccagattggctcgaggacaaccttagtgaaggaattcgcg 7mer peptide agtggtgggctttgaaacctggagcccctcaacccaaggcaaatcaacaacatcaagacaa underlined cgctcgaggtcttgtgcttccgggttacaaataccttggacccggcaacggactcgacaag ggggagccggtcaacgcagcagacgcggcggccctcgagcacgacaaggcctacgaccagc agctcaaggccggagacaacccgtacctcaagtacaaccacgccgacgccgagttccagga gcggctcaaagaagatacgtcttttgggggcaacctcgggcgagcagtcttccaggccaaa aagaggcttcttgaacctcttggtctggttgaggaagcggctaagacggctcctggaaaga agaggcctgtagagcagtctcctcaggaaccggactcctccgcgggtattggcaaatcggg tgcacagcccgctaaaaagagactcaatttcggtcagactggcgacacagagtcagtccca gaccctcaaccaatcggagaacctcccgcagccccctcaggtgtgggatctcttacaatgg cttcaggtggtggcgcaccagtggcagacaataacgaaggtgccgatggagtgggtagttc ctcgggaaattggcattgcgattcccaatggctgggggacagagtcatcaccaccagcacc cgaacctgggccctgcccacctacaacaatcacctctacaagcaaatctccaacagcacat ctggaggatcttcaaatgacaacgcctacttcggctacagcaccccctgggggtattttga cttcaacagattccactgccacttctcaccacgtgactggcagcgactcatcaacaacaac tggggattccggcctaagcgactcaacttcaagctcttcaacattcaggtcaaagaggtta cggacaacaatggagtcaagaccatcgccaataaccttaccagcacggtccaggtcttcac ggactcagactatcagctcccgtacgtgctcgggtcggctcacgagggctgcctcccgccg ttcccagcggacgttttcatgattcctcagtacgggtatctgacgcttaatgatggaagcc aggccgtgggtcgttcgtccttttactgcctggaatatttcccgtcgcaaatgctaagaac gggtaacaacttccagttcagctacgagtttgagaacgtacctttccatagcagctacgct cacagccaaagcctggaccgactaatgaatccactcatcgaccaatacttgtactatctct caaagactattaacggttctggacagaatcaacaaacgctaaaattcagtgtggccggacc cagcaacatggctgtccagggaagaaactacatacctggacccagctaccgacaacaacgt gtctcaaccactgtgactcaaaacaacaacagcgaatttgcttggcctggagcttcttctt gggctctcaatggacgtaatagcttgatgaatcctggacctgctatggccagccacaaaga aggagaggaccgtttctttcctttgtctggatctttaatttttggcaaacaaggaactgga agagacaacgtggatgcggacaaagtcatgataaccaacgaagaagaaattaaaactacta acccggtagcaacggagtcctatggacaagtggccacaaaccaccagagtgcacaggct ta ttctacggatgtgaggatgca ggcgcagaccggctgggttcaaaaccaaggaatacttccg ggtatggtttggcaggacagagatgtgtacctgcaaggacccatttgggccaaaattcctc acacggacggcaactttcacccttctccgctgatgggagggtttggaatgaagcacccgcc tcctcagatcctcatcaaaaacacacctgtacctgcCgatcctccaacggccttcaacaag gacaagctgaactctttcatcacccagtattctactggccaagtcagcgtggagatcgagt gggagctgcagaaggaaaacagcaagcgOtggaacccggagatccagtacacttccaacta ttacaagtctaataatgttgaatttgctgttaatactgaaggtgtatatagtgaaccccgc cccattggcaccagatacctgactcgtaatctgtaa TTD-004 3627 atggctgccgatggttatcttccagattggctcgaggacaaccttagtgaaggaattcgcg 7mer peptide agtggtgggctttgaaacctggagcccctcaacccaaggcaaatcaacaacatcaagacaa underlined cgctcgaggtcttgtgcttccgggttacaaataccttggacccggcaacggactcgacaag ggggagccggtcaacgcagcagacgcggcggccctcgagcacgacaaggcctacgaccagc agctcaaggccggagacaacccgtacctcaagtacaaccacgccgacgccgagttccagga gcggctcaaagaagatacgtcttttgggggcaacctcgggcgagcagtcttccaggccaaa aagaggcttcttgaacctcttggtctggttgaggaagcggctaagacggctcctggaaaga agaggcctgtagagcagtctcctcaggaaccggactcctccgcgggtattggcaaatcggg tgcacagcccgctaaaaagagactcaatttcggtcagactggcgacacagagtcagtccca gaccctcaaccaatcggagaacctcccgcagccccctcaggtgtgggatctcttacaatgg cttcaggtggtggcgcaccagtggcagacaataacgaaggtgccgatggagtgggtagttc ctcgggaaattggcattgcgattcccaatggctgggggacagagtcatcaccaccagcacc cgaacctgggccctgcccacctacaacaatcacctctacaagcaaatctccaacagcacat ctggaggatcttcaaatgacaacgcctacttcggctacagcaccccctgggggtattttga cttcaacagattccactgccacttctcaccacgtgactggcagcgactcatcaacaacaac tggggattccggcctaagcgactcaacttcaagctcttcaacattcaggtcaaagaggtta cggacaacaatggagtcaagaccatcgccaataaccttaccagcacggtccaggtcttcac ggactcagactatcagctcccgtacgtgctcgggtcggctcacgagggctgcctcccgccg ttcccagcggacgttttcatgattcctcagtacgggtatctgacgcttaatgatggaagcc aggccgtgggtcgttcgtccttttactgcctggaatatttcccgtcgcaaatgctaagaac gggtaacaacttccagttcagctacgagtttgagaacgtacctttccatagcagctacgct cacagccaaagcctggaccgactaatgaatccactcatcgaccaatacttgtactatctct caaagactattaacggttctggacagaatcaacaaacgctaaaattcagtgtggccggacc cagcaacatggctgtccagggaagaaactacatacctggacccagctaccgacaacaacgt gtctcaaccactgtgactcaaaacaacaacagcgaatttgcttggcctggagcttcttctt gggctctcaatggacgtaatagcttgatgaatcctggacctgctatggccagccacaaaga aggagaggaccgtttctttcctttgtctggatctttaatttttggcaaacaaggaactgga agagacaacgtggatgcggacaaagtcatgataaccaacgaagaagaaattaaaactacta acccggtagcaacggagtcctatggacaagtggccacaaaccaccagagtgcacag attgt tatgaattcgttgaaggc tcaggcgcagaccggctgggttcaaaaccaaggaatacttccg ggtatggtttggcaggacagagatgtgtacctgcaaggacccatttgggccaaaattcctc acacggacggcaactttcacccttctccgctgatgggagggtttggaatgaagcacccgcc tcctcagatcctcatcaaaaacacacctgtacctgcCgatcctccaacggccttcaacaag gacaagctgaactctttcatcacccagtattctactggccaagtcagcgtggagatcgagt gggagctgcagaaggaaaacagcaagcgOtggaacccggagatccagtacacttccaacta ttacaagtctaataatgttgaatttgctgttaatactgaaggtgtatatagtgaaccccgc cccattggcaccagatacctgactcgtaatctgtaa TTD-005 3628 atggctgccgatggttatcttccagattggctcgaggacaaccttagtgaaggaattcgcg 7mer peptide agtggtgggctttgaaacctggagcccctcaacccaaggcaaatcaacaacatcaagacaa underlined cgctcgaggtcttgtgcttccgggttacaaataccttggacccggcaacggactcgacaag ggggagccggtcaacgcagcagacgcggcggccctcgagcacgacaaggcctacgaccagc agctcaaggccggagacaacccgtacctcaagtacaaccacgccgacgccgagttccagga gcggctcaaagaagatacgtcttttgggggcaacctcgggcgagcagtcttccaggccaaa aagaggcttcttgaacctcttggtctggttgaggaagcggctaagacggctcctggaaaga agaggcctgtagagcagtctcctcaggaaccggactcctccgcgggtattggcaaatcggg tgcacagcccgctaaaaagagactcaatttcggtcagactggcgacacagagtcagtccca gaccctcaaccaatcggagaacctcccgcagccccctcaggtgtgggatctcttacaatgg cttcaggtggtggcgcaccagtggcagacaataacgaaggtgccgatggagtgggtagttc ctcgggaaattggcattgcgattcccaatggctgggggacagagtcatcaccaccagcacc cgaacctgggccctgcccacctacaacaatcacctctacaagcaaatctccaacagcacat ctggaggatcttcaaatgacaacgcctacttcggctacagcaccccctgggggtattttga cttcaacagattccactgccacttctcaccacgtgactggcagcgactcatcaacaacaac tggggattccggcctaagcgactcaacttcaagctcttcaacattcaggtcaaagaggtta cggacaacaatggagtcaagaccatcgccaataaccttaccagcacggtccaggtcttcac ggactcagactatcagctcccgtacgtgctcgggtcggctcacgagggctgcctcccgccg ttcccagcggacgttttcatgattcctcagtacgggtatctgacgcttaatgatggaagcc aggccgtgggtcgttcgtccttttactgcctggaatatttcccgtcgcaaatgctaagaac gggtaacaacttccagttcagctacgagtttgagaacgtacctttccatagcagctacgct cacagccaaagcctggaccgactaatgaatccactcatcgaccaatacttgtactatctct caaagactattaacggttctggacagaatcaacaaacgctaaaattcagtgtggccggacc cagcaacatggctgtccagggaagaaactacatacctggacccagctaccgacaacaacgt gtctcaaccactgtgactcaaaacaacaacagcgaatttgcttggcctggagcttcttctt gggctctcaatggacgtaatagcttgatgaatcctggacctgctatggccagccacaaaga aggagaggaccgtttctttcctttgtctggatctttaatttttggcaaacaaggaactgga agagacaacgtggatgcggacaaagtcatgataaccaacgaagaagaaattaaaactacta acccggtagcaacggagtcctatggacaagtggccacaaaccaccagagtgcacaggct cg ggagagtcctcgtgggctgca ggcgcagaccggctgggttcaaaaccaaggaatacttccg ggtatggtttggcaggacagagatgtgtacctgcaaggacccatttgggccaaaattcctc acacggacggcaactttcacccttctccgctgatgggagggtttggaatgaagcacccgcc tcctcagatcctcatcaaaaacacacctgtacctgcCgatcctccaacggccttcaacaag gacaagctgaactctttcatcacccagtattctactggccaagtcagcgtggagatcgagt gggagctgcagaaggaaaacagcaagcgOtggaacccggagatccagtacacttccaacta ttacaagtctaataatgttgaatttgctgttaatactgaaggtgtatatagtgaaccccgc cccattggcaccagatacctgactcgtaatctgtaa TTD-006 3629 atggctgccgatggttatcttccagattggctcgaggacaaccttagtgaaggaattcgcg 7mer peptide agtggtgggctttgaaacctggagcccctcaacccaaggcaaatcaacaacatcaagacaa underlined cgctcgaggtcttgtgcttccgggttacaaataccttggacccggcaacggactcgacaag ggggagccggtcaacgcagcagacgcggcggccctcgagcacgacaaggcctacgaccagc agctcaaggccggagacaacccgtacctcaagtacaaccacgccgacgccgagttccagga gcggctcaaagaagatacgtcttttgggggcaacctcgggcgagcagtcttccaggccaaa aagaggcttcttgaacctcttggtctggttgaggaagcggctaagacggctcctggaaaga agaggcctgtagagcagtctcctcaggaaccggactcctccgcgggtattggcaaatcggg tgcacagcccgctaaaaagagactcaatttcggtcagactggcgacacagagtcagtccca gaccctcaaccaatcggagaacctcccgcagccccctcaggtgtgggatctcttacaatgg cttcaggtggtggcgcaccagtggcagacaataacgaaggtgccgatggagtgggtagttc ctcgggaaattggcattgcgattcccaatggctgggggacagagtcatcaccaccagcacc cgaacctgggccctgcccacctacaacaatcacctctacaagcaaatctccaacagcacat ctggaggatcttcaaatgacaacgcctacttcggctacagcaccccctgggggtattttga cttcaacagattccactgccacttctcaccacgtgactggcagcgactcatcaacaacaac tggggattccggcctaagcgactcaacttcaagctcttcaacattcaggtcaaagaggtta cggacaacaatggagtcaagaccatcgccaataaccttaccagcacggtccaggtcttcac ggactcagactatcagctcccgtacgtgctcgggtcggctcacgagggctgcctcccgccg ttcccagcggacgttttcatgattcctcagtacgggtatctgacgcttaatgatggaagcc aggccgtgggtcgttcgtccttttactgcctggaatatttcccgtcgcaaatgctaagaac gggtaacaacttccagttcagctacgagtttgagaacgtacctttccatagcagctacgct cacagccaaagcctggaccgactaatgaatccactcatcgaccaatacttgtactatctct caaagactattaacggttctggacagaatcaacaaacgctaaaattcagtgtggccggacc cagcaacatggctgtccagggaagaaactacatacctggacccagctaccgacaacaacgt gtctcaaccactgtgactcaaaacaacaacagcgaatttgcttggcctggagcttcttctt gggctctcaatggacgtaatagcttgatgaatcctggacctgctatggccagccacaaaga aggagaggaccgtttctttcctttgtctggatctttaatttttggcaaacaaggaactgga agagacaacgtggatgcggacaaagtcatgataaccaacgaagaagaaattaaaactacta acccggtagcaacggagtcctatggacaagtggccacaaaccaccagagtgcacaggct ag ttttaatgatactagggctca ggcgcagaccggctgggttcaaaaccaaggaatacttccg ggtatggtttggcaggacagagatgtgtacctgcaaggacccatttgggccaaaattcctc acacggacggcaactttcacccttctccgctgatgggagggtttggaatgaagcacccgcc tcctcagatcctcatcaaaaacacacctgtacctgcCgatcctccaacggccttcaacaag gacaagctgaactctttcatcacccagtattctactggccaagtcagcgtggagatcgagt gggagctgcagaaggaaaacagcaagcgOtggaacccggagatccagtacacttccaacta ttacaagtctaataatgttgaatttgctgttaatactgaaggtgtatatagtgaaccccgc cccattggcaccagatacctgactcgtaatctgtaa TTD-007 3630 atggctgccgatggttatcttccagattggctcgaggacaaccttagtgaaggaattcgcg 9mer peptide agtggtgggctttgaaacctggagcccctcaacccaaggcaaatcaacaacatcaagacaa underlined cgctcgaggtcttgtgcttccgggttacaaataccttggacccggcaacggactcgacaag ggggagccggtcaacgcagcagacgcggcggccctcgagcacgacaaggcctacgaccagc agctcaaggccggagacaacccgtacctcaagtacaaccacgccgacgccgagttccagga gcggctcaaagaagatacgtcttttgggggcaacctcgggcgagcagtcttccaggccaaa aagaggcttcttgaacctcttggtctggttgaggaagcggctaagacggctcctggaaaga agaggcctgtagagcagtctcctcaggaaccggactcctccgcgggtattggcaaatcggg tgcacagcccgctaaaaagagactcaatttcggtcagactggcgacacagagtcagtccca gaccctcaaccaatcggagaacctcccgcagccccctcaggtgtgggatctcttacaatgg cttcaggtggtggcgcaccagtggcagacaataacgaaggtgccgatggagtgggtagttc ctcgggaaattggcattgcgattcccaatggctgggggacagagtcatcaccaccagcacc cgaacctgggccctgcccacctacaacaatcacctctacaagcaaatctccaacagcacat ctggaggatcttcaaatgacaacgcctacttcggctacagcaccccctgggggtattttga cttcaacagattccactgccacttctcaccacgtgactggcagcgactcatcaacaacaac tggggattccggcctaagcgactcaacttcaagctcttcaacattcaggtcaaagaggtta cggacaacaatggagtcaagaccatcgccaataaccttaccagcacggtccaggtcttcac ggactcagactatcagctcccgtacgtgctcgggtcggctcacgagggctgcctcccgccg ttcccagcggacgttttcatgattcctcagtacgggtatctgacgcttaatgatggaagcc aggccgtgggtcgttcgtccttttactgcctggaatatttcccgtcgcaaatgctaagaac gggtaacaacttccagttcagctacgagtttgagaacgtacctttccatagcagctacgct cacagccaaagcctggaccgactaatgaatccactcatcgaccaatacttgtactatctct caaagactattaacggttctggacagaatcaacaaacgctaaaattcagtgtggccggacc cagcaacatggctgtccagggaagaaactacatacctggacccagctaccgacaacaacgt gtctcaaccactgtgactcaaaacaacaacagcgaatttgcttggcctggagcttcttctt gggctctcaatggacgtaatagcttgatgaatcctggacctgctatggccagccacaaaga aggagaggaccgtttctttcctttgtctggatctttaatttttggcaaacaaggaactgga agagacaacgtggatgcggacaaagtcatgataaccaacgaagaagaaattaaaactacta acccggtagcaacggagtcctatggacaagtggccacaaaccaccagagt ggtggtacgtt ggccgtcgtgtcgctt gctcaggcgcagaccggctgggttcaaaaccaaggaatacttccg ggtatggtttggcaggacagagatgtgtacctgcaaggacccatttgggccaaaattcctc acacggacggcaactttcacccttctccgctgatgggagggtttggaatgaagcacccgcc tcctcagatcctcatcaaaaacacacctgtacctgcCgatcctccaacggccttcaacaag gacaagctgaactctttcatcacccagtattctactggccaagtcagcgtggagatcgagt gggagctgcagaaggaaaacagcaagcgOtggaacccggagatccagtacacttccaacta ttacaagtctaataatgttgaatttgctgttaatactgaaggtgtatatagtgaaccccgc cccattggcaccagatacctgactcgtaatctgtaa TTD-008 3631 atggctgccgatggttatcttccagattggctcgaggacaaccttagtgaaggaattcgcg 7mer peptide agtggtgggctttgaaacctggagcccctcaacccaaggcaaatcaacaacatcaagacaa underlined cgctcgaggtcttgtgcttccgggttacaaataccttggacccggcaacggactcgacaag ggggagccggtcaacgcagcagacgcggcggccctcgagcacgacaaggcctacgaccagc agctcaaggccggagacaacccgtacctcaagtacaaccacgccgacgccgagttccagga gcggctcaaagaagatacgtcttttgggggcaacctcgggcgagcagtcttccaggccaaa aagaggcttcttgaacctcttggtctggttgaggaagcggctaagacggctcctggaaaga agaggcctgtagagcagtctcctcaggaaccggactcctccgcgggtattggcaaatcggg tgcacagcccgctaaaaagagactcaatttcggtcagactggcgacacagagtcagtccca gaccctcaaccaatcggagaacctcccgcagccccctcaggtgtgggatctcttacaatgg cttcaggtggtggcgcaccagtggcagacaataacgaaggtgccgatggagtgggtagttc ctcgggaaattggcattgcgattcccaatggctgggggacagagtcatcaccaccagcacc cgaacctgggccctgcccacctacaacaatcacctctacaagcaaatctccaacagcacat ctggaggatcttcaaatgacaacgcctacttcggctacagcaccccctgggggtattttga cttcaacagattccactgccacttctcaccacgtgactggcagcgactcatcaacaacaac tggggattccggcctaagcgactcaacttcaagctcttcaacattcaggtcaaagaggtta cggacaacaatggagtcaagaccatcgccaataaccttaccagcacggtccaggtcttcac ggactcagactatcagctcccgtacgtgctcgggtcggctcacgagggctgcctcccgccg ttcccagcggacgttttcatgattcctcagtacgggtatctgacgcttaatgatggaagcc aggccgtgggtcgttcgtccttttactgcctggaatatttcccgtcgcaaatgctaagaac gggtaacaacttccagttcagctacgagtttgagaacgtacctttccatagcagctacgct cacagccaaagcctggaccgactaatgaatccactcatcgaccaatacttgtactatctct caaagactattaacggttctggacagaatcaacaaacgctaaaattcagtgtggccggacc cagcaacatggctgtccagggaagaaactacatacctggacccagctaccgacaacaacgt gtctcaaccactgtgactcaaaacaacaacagcgaatttgcttggcctggagcttcttctt gggctctcaatggacgtaatagcttgatgaatcctggacctgctatggccagccacaaaga aggagaggaccgtttctttcctttgtctggatctttaatttttggcaaacaaggaactgga agagacaacgtggatgcggacaaagtcatgataaccaacgaagaagaaattaaaactacta acccggtagcaacggagtcctatggacaagtggccacaaaccaccagagtgcacaggct ta tgggttgccgaagggtcct caggcgcagaccggctgggttcaaaaccaaggaatacttccg ggtatggtttggcaggacagagatgtgtacctgcaaggacccatttgggccaaaattcctc acacggacggcaactttcacccttctccgctgatgggagggtttggaatgaagcacccgcc tcctcagatcctcatcaaaaacacacctgtacctgcCgatcctccaacggccttcaacaag gacaagctgaactctttcatcacccagtattctactggccaagtcagcgtggagatcgagt gggagctgcagaaggaaaacagcaagcgOtggaacccggagatccagtacacttccaacta ttacaagtctaataatgttgaatttgctgttaatactgaaggtgtatatagtgaaccccgc cccattggcaccagatacctgactcgtaatctgtaa TTD-009 3632 atggctgccgatggttatcttccagattggctcgaggacaaccttagtgaaggaattcgcg 7mer peptide agtggtgggctttgaaacctggagcccctcaacccaaggcaaatcaacaacatcaagacaa underlined cgctcgaggtcttgtgcttccgggttacaaataccttggacccggcaacggactcgacaag ggggagccggtcaacgcagcagacgcggcggccctcgagcacgacaaggcctacgaccagc agctcaaggccggagacaacccgtacctcaagtacaaccacgccgacgccgagttccagga gcggctcaaagaagatacgtcttttgggggcaacctcgggcgagcagtcttccaggccaaa aagaggcttcttgaacctcttggtctggttgaggaagcggctaagacggctcctggaaaga agaggcctgtagagcagtctcctcaggaaccggactcctccgcgggtattggcaaatcggg tgcacagcccgctaaaaagagactcaatttcggtcagactggcgacacagagtcagtccca gaccctcaaccaatcggagaacctcccgcagccccctcaggtgtgggatctcttacaatgg cttcaggtggtggcgcaccagtggcagacaataacgaaggtgccgatggagtgggtagttc ctcgggaaattggcattgcgattcccaatggctgggggacagagtcatcaccaccagcacc cgaacctgggccctgcccacctacaacaatcacctctacaagcaaatctccaacagcacat ctggaggatcttcaaatgacaacgcctacttcggctacagcaccccctgggggtattttga cttcaacagattccactgccacttctcaccacgtgactggcagcgactcatcaacaacaac tggggattccggcctaagcgactcaacttcaagctcttcaacattcaggtcaaagaggtta cggacaacaatggagtcaagaccatcgccaataaccttaccagcacggtccaggtcttcac ggactcagactatcagctcccgtacgtgctcgggtcggctcacgagggctgcctcccgccg ttcccagcggacgttttcatgattcctcagtacgggtatctgacgcttaatgatggaagcc aggccgtgggtcgttcgtccttttactgcctggaatatttcccgtcgcaaatgctaagaac gggtaacaacttccagttcagctacgagtttgagaacgtacctttccatagcagctacgct cacagccaaagcctggaccgactaatgaatccactcatcgaccaatacttgtactatctct caaagactattaacggttctggacagaatcaacaaacgctaaaattcagtgtggccggacc cagcaacatggctgtccagggaagaaactacatacctggacccagctaccgacaacaacgt gtctcaaccactgtgactcaaaacaacaacagcgaatttgcttggcctggagcttcttctt gggctctcaatggacgtaatagcttgatgaatcctggacctgctatggccagccacaaaga aggagaggaccgtttctttcctttgtctggatctttaatttttggcaaacaaggaactgga agagacaacgtggatgcggacaaagtcatgataaccaacgaagaagaaattaaaactacta acccggtagcaacggagtcctatggacaagtggccacaaaccaccagagtgcacaggct tc gactgggacgcttcggctt caggcgcagaccggctgggttcaaaaccaaggaatacttccg ggtatggtttggcaggacagagatgtgtacctgcaaggacccatttgggccaaaattcctc acacggacggcaactttcacccttctccgctgatgggagggtttggaatgaagcacccgcc tcctcagatcctcatcaaaaacacacctgtacctgcCgatcctccaacggccttcaacaag gacaagctgaactctttcatcacccagtattctactggccaagtcagcgtggagatcgagt gggagctgcagaaggaaaacagcaagcgOtggaacccggagatccagtacacttccaacta ttacaagtctaataatgttgaatttgctgttaatactgaaggtgtatatagtgaaccccgc cccattggcaccagatacctgactcgtaatctgtaa TTD-010 3633 atggctgccgatggttatcttccagattggctcgaggacaaccttagtgaaggaattcgcg 7mer peptide agtggtgggctttgaaacctggagcccctcaacccaaggcaaatcaacaacatcaagacaa underlined cgctcgaggtcttgtgcttccgggttacaaataccttggacccggcaacggactcgacaag ggggagccggtcaacgcagcagacgcggcggccctcgagcacgacaaggcctacgaccagc agctcaaggccggagacaacccgtacctcaagtacaaccacgccgacgccgagttccagga gcggctcaaagaagatacgtcttttgggggcaacctcgggcgagcagtcttccaggccaaa aagaggcttcttgaacctcttggtctggttgaggaagcggctaagacggctcctggaaaga agaggcctgtagagcagtctcctcaggaaccggactcctccgcgggtattggcaaatcggg tgcacagcccgctaaaaagagactcaatttcggtcagactggcgacacagagtcagtccca gaccctcaaccaatcggagaacctcccgcagccccctcaggtgtgggatctcttacaatgg cttcaggtggtggcgcaccagtggcagacaataacgaaggtgccgatggagtgggtagttc ctcgggaaattggcattgcgattcccaatggctgggggacagagtcatcaccaccagcacc cgaacctgggccctgcccacctacaacaatcacctctacaagcaaatctccaacagcacat ctggaggatcttcaaatgacaacgcctacttcggctacagcaccccctgggggtattttga cttcaacagattccactgccacttctcaccacgtgactggcagcgactcatcaacaacaac tggggattccggcctaagcgactcaacttcaagctcttcaacattcaggtcaaagaggtta cggacaacaatggagtcaagaccatcgccaataaccttaccagcacggtccaggtcttcac ggactcagactatcagctcccgtacgtgctcgggtcggctcacgagggctgcctcccgccg ttcccagcggacgttttcatgattcctcagtacgggtatctgacgcttaatgatggaagcc aggccgtgggtcgttcgtccttttactgcctggaatatttcccgtcgcaaatgctaagaac gggtaacaacttccagttcagctacgagtttgagaacgtacctttccatagcagctacgct cacagccaaagcctggaccgactaatgaatccactcatcgaccaatacttgtactatctct caaagactattaacggttctggacagaatcaacaaacgctaaaattcagtgtggccggacc cagcaacatggctgtccagggaagaaactacatacctggacccagctaccgacaacaacgt gtctcaaccactgtgactcaaaacaacaacagcgaatttgcttggcctggagcttcttctt gggctctcaatggacgtaatagcttgatgaatcctggacctgctatggccagccacaaaga aggagaggaccgtttctttcctttgtctggatctttaatttttggcaaacaaggaactgga agagacaacgtggatgcggacaaagtcatgataaccaacgaagaagaaattaaaactac ta acccggtagcaacggagtcctatggacaagtggccacaaaccaccagagtgcgcaggcgta ttcgacggatgagaggatg caggcgcagaccggctgggttcaaaaccaaggaatacttccg ggtatggtttggcaggacagagatgtgtacctgcaaggacccatttgggccaaaattcctc acacggacggcaactttcacccttctccgctgatgggagggtttggaatgaagcacccgcc tcctcagatcctcatcaaaaacacacctgtacctgcCgatcctccaacggccttcaacaag gacaagctgaactctttcatcacccagtattctactggccaagtcagcgtggagatcgagt gggagctgcagaaggaaaacagcaagcgOtggaacccggagatccagtacacttccaacta ttacaagtctaataatgttgaatttgctgttaatactgaaggtgtatatagtgaaccccgc cccattggcaccagatacctgactcgtaatctgtaa TTD-011 3634 atggctgccgatggttatcttccagattggctcgaggacaaccttagtgaaggaattcgcg 7mer peptide agtggtgggctttgaaacctggagcccctcaacccaaggcaaatcaacaacatcaagacaa underlined cgctcgaggtcttgtgcttccgggttacaaataccttggacccggcaacggactcgacaag ggggagccggtcaacgcagcagacgcggcggccctcgagcacgacaaggcctacgaccagc agctcaaggccggagacaacccgtacctcaagtacaaccacgccgacgccgagttccagga gcggctcaaagaagatacgtcttttgggggcaacctcgggcgagcagtcttccaggccaaa aagaggcttcttgaacctcttggtctggttgaggaagcggctaagacggctcctggaaaga agaggcctgtagagcagtctcctcaggaaccggactcctccgcgggtattggcaaatcggg tgcacagcccgctaaaaagagactcaatttcggtcagactggcgacacagagtcagtccca gaccctcaaccaatcggagaacctcccgcagccccctcaggtgtgggatctcttacaatgg cttcaggtggtggcgcaccagtggcagacaataacgaaggtgccgatggagtgggtagttc ctcgggaaattggcattgcgattcccaatggctgggggacagagtcatcaccaccagcacc cgaacctgggccctgcccacctacaacaatcacctctacaagcaaatctccaacagcacat ctggaggatcttcaaatgacaacgcctacttcggctacagcaccccctgggggtattttga cttcaacagattccactgccacttctcaccacgtgactggcagcgactcatcaacaacaac tggggattccggcctaagcgactcaacttcaagctcttcaacattcaggtcaaagaggtta cggacaacaatggagtcaagaccatcgccaataaccttaccagcacggtccaggtcttcac ggactcagactatcagctcccgtacgtgctcgggtcggctcacgagggctgcctcccgccg ttcccagcggacgttttcatgattcctcagtacgggtatctgacgcttaatgatggaagcc aggccgtgggtcgttcgtccttttactgcctggaatatttcccgtcgcaaatgctaagaac gggtaacaacttccagttcagctacgagtttgagaacgtacctttccatagcagctacgct cacagccaaagcctggaccgactaatgaatccactcatcgaccaatacttgtactatctct caaagactattaacggttctggacagaatcaacaaacgctaaaattcagtgtggccggacc cagcaacatggctgtccagggaagaaactacatacctggacccagctaccgacaacaacgt gtctcaaccactgtgactcaaaacaacaacagcgaatttgcttggcctggagcttcttctt gggctctcaatggacgtaatagcttgatgaatcctggacctgctatggccagccacaaaga aggagaggaccgtttctttcctttgtctggatctttaatttttggcaaacaaggaactgga agagacaacgtggatgcggacaaagtcatgataaccaacgaagaagaaattaaaactacta acccggtagcaacggagtcctatggacaagtggccacaaaccaccagagtgcgcaggcg ta ttcgacggatgagaggaag caggcgcagaccggctgggttcaaaaccaaggaatacttccg ggtatggtttggcaggacagagatgtgtacctgcaaggacccatttgggccaaaattcctc acacggacggcaactttcacccttctccgctgatgggagggtttggaatgaagcacccgcc tcctcagatcctcatcaaaaacacacctgtacctgcCgatcctccaacggccttcaacaag gacaagctgaactctttcatcacccagtattctactggccaagtcagcgtggagatcgagt gggagctgcagaaggaaaacagcaagcgOtggaacccggagatccagtacacttccaacta ttacaagtctaataatgttgaatttgctgttaatactgaaggtgtatatagtgaaccccgc cccattggcaccagatacctgactcgtaatctgtaa TTD-012 3635 atggctgccgatggttatcttccagattggctcgaggacaaccttagtgaaggaattcgcg 7mer peptide agtggtgggctttgaaacctggagcccctcaacccaaggcaaatcaacaacatcaagacaa underlined cgctcgaggtcttgtgcttccgggttacaaataccttggacccggcaacggactcgacaag ggggagccggtcaacgcagcagacgcggcggccctcgagcacgacaaggcctacgaccagc agctcaaggccggagacaacccgtacctcaagtacaaccacgccgacgccgagttccagga gcggctcaaagaagatacgtcttttgggggcaacctcgggcgagcagtcttccaggccaaa aagaggcttcttgaacctcttggtctggttgaggaagcggctaagacggctcctggaaaga agaggcctgtagagcagtctcctcaggaaccggactcctccgcgggtattggcaaatcggg tgcacagcccgctaaaaagagactcaatttcggtcagactggcgacacagagtcagtccca gaccctcaaccaatcggagaacctcccgcagccccctcaggtgtgggatctcttacaatgg cttcaggtggtggcgcaccagtggcagacaataacgaaggtgccgatggagtgggtagttc ctcgggaaattggcattgcgattcccaatggctgggggacagagtcatcaccaccagcacc cgaacctgggccctgcccacctacaacaatcacctctacaagcaaatctccaacagcacat ctggaggatcttcaaatgacaacgcctacttcggctacagcaccccctgggggtattttga cttcaacagattccactgccacttctcaccacgtgactggcagcgactcatcaacaacaac tggggattccggcctaagcgactcaacttcaagctcttcaacattcaggtcaaagaggtta cggacaacaatggagtcaagaccatcgccaataaccttaccagcacggtccaggtcttcac ggactcagactatcagctcccgtacgtgctcgggtcggctcacgagggctgcctcccgccg ttcccagcggacgttttcatgattcctcagtacgggtatctgacgcttaatgatggaagcc aggccgtgggtcgttcgtccttttactgcctggaatatttcccgtcgcaaatgctaagaac gggtaacaacttccagttcagctacgagtttgagaacgtacctttccatagcagctacgct cacagccaaagcctggaccgactaatgaatccactcatcgaccaatacttgtactatctct caaagactattaacggttctggacagaatcaacaaacgctaaaattcagtgtggccggacc cagcaacatggctgtccagggaagaaactacatacctggacccagctaccgacaacaacgt gtctcaaccactgtgactcaaaacaacaacagcgaatttgcttggcctggagcttcttctt gggctctcaatggacgtaatagcttgatgaatcctggacctgctatggccagccacaaaga aggagaggaccgtttctttcctttgtctggatctttaatttttggcaaacaaggaactgga agagacaacgtggatgcggacaaagtcatgataaccaacgaagaagaaattaaaactacta acccggtagcaacggagtcctatggacaagtggccacaaaccaccagagtgcacaggct ta tgtttcgtctgttaagatg caggcgcagaccggctgggttcaaaaccaaggaatacttccg ggtatggtttggcaggacagagatgtgtacctgcaaggacccatttgggccaaaattcctc acacggacggcaactttcacccttctccgctgatgggagggtttggaatgaagcacccgcc tcctcagatcctcatcaaaaacacacctgtacctgcCgatcctccaacggccttcaacaag gacaagctgaactctttcatcacccagtattctactggccaagtcagcgtggagatcgagt gggagctgcagaaggaaaacagcaagcgOtggaacccggagatccagtacacttccaacta ttacaagtctaataatgttgaatttgctgttaatactgaaggtgtatatagtgaaccccgc cccattggcaccagatacctgactcgtaatctgtaa

In some embodiments, the polynucleotide encoding an AAV capsid polypeptide, e.g., AAV capsid variant, described herein comprises the nucleotide sequence of any one of SEQ ID NOs: 3623-3635, or a nucleotide sequence with at least 80% (e.g., at least about 85, 90, 95, 96, 97, 98, or 99%) sequence identity thereto. In some embodiments, the polynucleotide encoding an AAV capsid variant described herein comprises the nucleotide sequence of SEQ ID NO: 3623, or a nucleotide sequence with at least 80% (e.g., at least about 85, 90, 95, 96, 97, 98, or 99%) sequence identity thereto. In some embodiments, the polynucleotide encoding an AAV capsid variant described herein comprises the nucleotide sequence of SEQ ID NO: 3627, or a nucleotide sequence with at least 80% (e.g., at least about 85, 90, 95, 96, 97, 98, or 99%) sequence identity thereto. In some embodiments, the nucleic acid sequence encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein is codon optimized.

In some embodiments, a polynucleotide encoding the AAV capsid polypeptide, e.g., the AAV capsid variant, (e.g., VP1) of an AAV particle may comprise a nucleic acid sequence with 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identity to any of those described herein or provided by any of SEQ ID NO: 3623-3635.

In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, (e.g., VP1) may comprise a nucleic acid sequence having at least 80% identity to any of SEQ ID NO: 3623-3635. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, (e.g., VP1) may comprise a nucleic acid sequence having at least 85% identity to any of SEQ ID NO: 3623-3635. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, (e.g., VP1) is encoded by a nucleic acid sequence having at least 90% identity to any of SEQ ID NO: 3623-3635. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, (e.g., VP1) may comprise a nucleic acid sequence having at least 95% identity to any of SEQ ID NO: 3623-3635. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, (e.g., VP1) may comprise a nucleic acid sequence having at least 96% identity to any of SEQ ID NO: 3623-3635. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, (e.g., VP1) may comprise a nucleic acid sequence having at least 97% identity to any of SEQ ID NO: 3623-3635. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant. (e.g., VP1) may comprise a nucleic acid sequence having at least 98% identity to any of SEQ ID NO: 3623-3635. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, (e.g., VP1) may comprise a nucleic acid sequence having at least 99% identity to any of SEQ ID NO: 3623-3635. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, (e.g., VP1) may comprise a nucleic acid sequence given by any of SEQ ID NO: 3623-3635.

In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 80% identity to SEQ ID NO: 3623. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 85% identity to SEQ ID NO: 3623. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 90% identity to SEQ ID NO: 3623. In some embodiments, an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 95% identity to SEQ ID NO: 3623. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 96% identity to SEQ ID NO: 3623. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 97% identity to SEQ ID NO: 3623. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 98% identity to SEQ ID NO: 3623. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 99% identity to SEQ ID NO: 3623.

In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may have a nucleic acid sequence comprising SEQ ID NO: 3623.

In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may have a nucleic acid sequence consisting of SEQ ID NO: 3623.

In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 80% identity to SEQ ID NO: 3624 or 3625. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 85% identity to SEQ ID NO: 3624 or 3625. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 90% identity to SEQ ID NO: 3624 or 3625. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 95% identity to SEQ ID NO: 3624 or 3625. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 96% identity to SEQ ID NO: 3624 or 3625. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 97% identity to SEQ ID NO: 3624 or 3625. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 98% identity to SEQ ID NO: 3624 or 3625. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 99% identity to SEQ ID NO: 3624 or 3625.

In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may have a nucleic acid sequence comprising SEQ ID NO: 3624 or 3625.

In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may have a nucleic acid sequence consisting of SEQ ID NO: 3624 or 3625.

In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 80% identity to SEQ ID NO: 3626. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 85% identity to SEQ ID NO: 3626. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 90% identity to SEQ ID NO: 3626. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 95% identity to SEQ ID NO: 3626. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 96% identity to SEQ ID NO: 3626. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 97% identity to SEQ ID NO: 3626. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 98% identity to SEQ ID NO: 3626. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 99% identity to SEQ ID NO: 3626.

In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may have a nucleic acid sequence comprising SEQ ID NO: 3626.

In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may have a nucleic acid sequence consisting of SEQ ID NO: 3626.

In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 80% identity to SEQ ID NO: 3627. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 85% identity to SEQ ID NO: 3627. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 90% identity to SEQ ID NO: 3627. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 95% identity to SEQ ID NO: 3627. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 96% identity to SEQ ID NO: 3627. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 97% identity to SEQ ID NO: 3627. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 98% identity to SEQ ID NO: 3627. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 99% identity to SEQ ID NO: 3627.

In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may have a nucleic acid sequence comprising SEQ ID NO: 3627.

In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may have a nucleic acid sequence consisting of SEQ ID NO: 3627.

In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 80% identity to SEQ ID NO: 3628. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 85% identity to SEQ ID NO: 3628. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 90% identity to SEQ ID NO: 3628. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 95% identity to SEQ ID NO: 3628. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 96% identity to SEQ ID NO: 3628. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 97% identity to SEQ ID NO: 3628. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 98% identity to SEQ ID NO: 3628. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may comprise a nucleic acid sequence having at least 99% identity to SEQ ID NO: 3628.

In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may have a nucleic acid sequence comprising SEQ ID NO: 3628.

In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, may have a nucleic acid sequence consisting of SEQ ID NO: 3628.

In some embodiments, the AAV capsid polypeptide, e.g., the AAV capsid variant, comprises a VP2 protein comprising the amino acid sequence corresponding to positions 138-743, of any one of SEQ ID NOs: 3636-3647, or a sequence with at least 80% (e.g., at least about 85, 90, 95, 96, 97, 98, or 99%) sequence identity thereto. In some embodiments, the AAV capsid comprises a VP3 protein comprising the amino acid sequence corresponding to positions 203-743, of any one of SEQ ID NOs: 3636-3647, or a sequence with at least 80% (e.g., at least about 85, 90, 95, 96, 97, 98, or 99%) sequence identity thereto.

In some embodiments, the AAV capsid polypeptide, e.g., the AAV capsid variant, e.g., an AAV capsid variant described herein, comprises the amino acid sequence of any one of SEQ ID NOs: 3636-3647, or an amino acid sequence with at least 80% (e.g., at least about 85, 90, 95, 96, 97, 98, or 99%) sequence identity thereto. In some embodiments, the AAV capsid polypeptide, e.g., the AAV capsid variant, e.g., an AAV capsid variant described herein, comprises an amino acid sequence having at least one, two, or three modifications, but not more than 30, 20 or 10 modifications of the amino acid sequence of any one of SEQ ID NOs: 3636-3647.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, (e.g., VP1) of an AAV particle may comprise an amino acid sequence with 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identity to any of those described herein or provided as any of SEQ ID NO: 3636-3647.

In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, (e.g., VP1) may comprise an amino acid sequence having at least 80% identity to any of SEQ ID NO: 3636-3647. In some embodiments, an AAV capsid (e.g., VP1) may comprise an amino acid sequence having at least 85% identity to any of SEQ ID NO: 3636-3647. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, (e.g., VP1) may comprise an amino acid sequence having at least 90% identity to any of SEQ ID NO: 3636-3647. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, (e.g., VP1) may comprise an amino acid sequence having at least 95% identity to any of SEQ ID NO: 3636-3647. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, (e.g., VP1) may comprise an amino acid sequence having at least 96% identity to any of SEQ ID NO: 3636-3647. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, (e.g., VP1) may comprise an amino acid sequence having at least 97% identity to any of SEQ ID NO: 3636-3647. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, (e.g., VP1) may comprise an amino acid sequence having at least 98% identity to any of SEQ ID NO: 3636-3647. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, (e.g., VP1) may comprise an amino acid sequence having at least 99% identity to any of SEQ ID NO: 3636-3647. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, (e.g., VP1) may have an amino acid sequence comprising any of SEQ ID NO: 3636-3647. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, (e.g., VP1) may have an amino acid sequence consisting of any of SEQ ID NO: 3636-3647.

In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 80% identity to SEQ ID NO: 3636. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 85% identity to SEQ ID NO: 3636. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 90% identity to SEQ ID NO: 3636. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 95% identity to SEQ ID NO: 3636. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 96% identity to SEQ ID NO: 3636. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 97% identity to SEQ ID NO: 3636. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 98% identity to SEQ ID NO: 3636. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 99% identity to SEQ ID NO: 3636.

In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may have an amino acid sequence comprising SEQ ID NO: 3636. In some embodiments, the AAV capsid variant comprises the amino acid sequence of SEQ ID NO: 3636.

In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may have an amino acid sequence consisting of SEQ ID NO: 3636.

In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, comprising an amino acid sequence given as SEQ ID NO: 3636 is encoded by a nucleic acid sequence comprising SEQ ID NO: 3623. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, comprising an amino acid sequence given as SEQ ID NO: 3636 is encoded by a codon-optimized nucleic acid sequence having 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identity to SEQ ID NO: 3623.

In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 80% identity to SEQ ID NO: 3637. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 85% identity to SEQ ID NO: 3637. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 90% identity to SEQ ID NO: 3637. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 95% identity to SEQ ID NO: 3637. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 96% identity to SEQ ID NO: 3637. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 97% identity to SEQ ID NO: 3637. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 98% identity to SEQ ID NO: 3637. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 99% identity to SEQ ID NO: 3637.

In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may have an amino acid sequence comprising SEQ ID NO: 3637. In some embodiments, the AAV capsid variant comprises the amino acid sequence of SEQ ID NO: 3637.

In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may have an amino acid sequence consisting of SEQ ID NO: 3637.

In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, comprising an amino acid sequence given as SEQ ID NO: 3637 is encoded by a nucleic acid sequence comprising SEQ ID NO: 3624 or 3625. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, comprising an amino acid sequence given as SEQ ID NO: 3637 is encoded by a codon-optimized nucleic acid sequence having 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identity to SEQ ID NO: 3624 or 3625.

In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 80% identity to SEQ ID NO: 3638. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 85% identity to SEQ ID NO: 3638. In some embodiments, an AAV capsid, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 90% identity to SEQ ID NO: 3638. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 95% identity to SEQ ID NO: 3638. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 96% identity to SEQ ID NO: 3638. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 97% identity to SEQ ID NO: 3638. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 98% identity to SEQ ID NO: 3638. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 99% identity to SEQ ID NO: 3638.

In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may have an amino acid sequence comprising SEQ ID NO: 3638. In some embodiments, the AAV capsid variant comprises the amino acid sequence of SEQ ID NO: 3638.

In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may have an amino acid sequence consisting of SEQ ID NO: 3638.

In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, comprising an amino acid sequence given as SEQ ID NO: 3638 is encoded by a nucleic acid sequence comprising SEQ ID NO: 3626. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, comprising an amino acid sequence given as SEQ ID NO: 3638 is encoded by a codon-optimized nucleic acid sequence having 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identity to SEQ ID NO: 3626.

In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 80% identity to SEQ ID NO: 3639. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 85% identity to SEQ ID NO: 3639. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 90% identity to SEQ ID NO: 3639. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 95% identity to SEQ ID NO: 3639. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 96% identity to SEQ ID NO: 3639. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 97% identity to SEQ ID NO: 3639. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 98% identity to SEQ ID NO: 3639. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 99% identity to SEQ ID NO: 3639.

In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may have an amino acid sequence comprising SEQ ID NO: 3639. In some embodiments, the AAV capsid variant comprises the amino acid sequence of SEQ ID NO: 3639.

In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may have an amino acid sequence consisting of SEQ ID NO: 3639.

In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, comprising an amino acid sequence given as SEQ ID NO: 3639 is encoded by a nucleic acid sequence comprising SEQ ID NO: 3627. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, comprising an amino acid sequence given as SEQ ID NO: 3639 is encoded by a codon-optimized nucleic acid sequence having 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identity to SEQ ID NO: 3627.

In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 80% identity to SEQ ID NO: 3640. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 85% identity to SEQ ID NO: 3640. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 90% identity to SEQ ID NO: 3640. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 95% identity to SEQ ID NO: 3640. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 96% identity to SEQ ID NO: 3640. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 97% identity to SEQ ID NO: 3640. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 98% identity to SEQ ID NO: 3640. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may comprise an amino acid sequence having at least 99% identity to SEQ ID NO: 3640.

In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may have an amino acid sequence comprising SEQ ID NO: 3640. In some embodiments, the AAV capsid variant comprises the amino acid sequence of SEQ ID NO: 3640.

In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, may have an amino acid sequence consisting of SEQ ID NO: 3640.

In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, comprising an amino acid sequence given as SEQ ID NO: 3640 is encoded by a nucleic acid sequence comprising SEQ ID NO: 3628. In some embodiments, an AAV capsid polypeptide, e.g., AAV capsid variant, comprising an amino acid sequence given as SEQ ID NO: 3640 is encoded by a codon-optimized nucleic acid sequence having 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identity to SEQ ID NO: 3628.

In some embodiments, an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein has an increased tropism for a CNS cell or tissue, e.g., a brain cell, brain tissue, spinal cord cell, or spinal cord tissue, relative to the tropism of a reference sequence comprising the amino acid sequence of SEQ ID NO: 138.

In some embodiments, an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein transduces a brain region, e.g., selected from dentate nucleus, cerebellar cortex, cerebral cortex, brain stem, hippocampus, thalamus and putamen. In some embodiments, the level of transduction of said brain region is at least 5, 10, 50, 100, 200, 500, 1,000, 2,000, 5,000, or 10,000-fold greater as compared to a reference sequence of SEQ ID NO: 138.

In some embodiments, an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein is enriched at least about 5, 6, 7, 8, 9, or 10-fold, in the brain compared to a reference sequence of SEQ ID NO: 138. In some embodiments, an AAV capsid variant described herein is enriched at least about 20, 30, 40, or 50-fold in the brain compared to a reference sequence of SEQ ID NO: 138. In some embodiments, an AAV capsid variant described herein is enriched at least about 100, 200, 300, or 400-fold in the brain compared to a reference sequence of SEQ ID NO: 138.

In some embodiments, an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein delivers an increased level of viral genomes to a brain region. In some embodiments, the level of viral genomes is increased by at least 5, 10, 20, 30, 40 or 50-fold, as compared to a reference sequence of SEQ ID NO: 138. In some embodiments, the brain region comprises a frontal cortex, sensory cortex, motor cortex, putamen, thalamus, cerebellar cortex, dentate nucleus, caudate, and/or hippocampus.

In some embodiments, an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein delivers an increased level of a payload to a brain region. In some embodiments, the level of the payload is increased by at least 5, 10, 50, 100, 200, 500, 1,000, 2,000, 5,000, or 10,000-fold, as compared to a reference sequence of SEQ ID NO: 138. In some embodiments, the brain region comprises a frontal cortex, sensory cortex, motor cortex, putamen, thalamus, cerebellar cortex, dentate nucleus, caudate, and/or hippocampus.

In some embodiments, an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein delivers an increased level of a payload to a spinal cord region. In some embodiments, the level of the payload is increased by at least 10, 20, 50, 100, 200, 300, 400, 500, 600, 700, 800 or 900-fold, as compared to a reference sequence of SEQ ID NO: 138. In some embodiments, the spinal cord region comprises a cervical, thoracic, and/or lumbar region.

In some embodiments, an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein shows preferential transduction in a brain region relative to the transduction in the dorsal root ganglia (DRG).

In some embodiments, an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein has an increased tropism for a muscle cell or tissue, e.g., a heart cell or tissue, relative to the tropism of a reference sequence comprising the amino acid sequence of SEQ ID NO: 138. In some embodiments, the AAV capsid variant delivers an increased level of a payload to a muscle region. In some embodiments, the payload is increased by at least 10, 15, 20, 30, or 40-fold, as compared to a reference sequence of SEQ ID NO: 138. In some embodiments, the muscle region comprises a heart muscle, quadriceps muscle, and/or a diaphragm muscle region. In some embodiments, the muscle region comprises a heart muscle region, e.g., a heart atrium muscle region or a heart ventricle muscle region.

In some embodiments, an, AAV capsid polypeptide, e.g., an AAV capsid variant, of the present disclosure is isolated, e.g., recombinant. In some embodiments, a polynucleotide encoding an AAV capsid polypeptide, e.g., an AAV capsid variant, of the present disclosure is isolated, e.g., recombinant.

In any of the DNA and RNA sequences referenced and/or described herein, the single letter symbol has the following description: A for adenine; C for cytosine; G for guanine; T for thymine; U for Uracil; W for weak bases such as adenine or thymine; S for strong nucleotides such as cytosine and guanine; M for amino nucleotides such as adenine and cytosine; K for keto nucleotides such as guanine and thymine; R for purines adenine and guanine; Y for pyrimidine cytosine and thymine; B for any base that is not A (e.g., cytosine, guanine, and thymine); D for any base that is not C (e.g., adenine, guanine, and thymine); H for any base that is not G (e.g., adenine, cytosine, and thymine); V for any base that is not T (e.g., adenine, cytosine, and guanine); N for any nucleotide (which is not a gap); and Z is for zero.

In any of the amino acid sequences referenced and/or described herein, the single letter symbol has the following description: G (Gly) for Glycine; A (Ala) for Alanine; L (Leu) for Leucine; M (Met) for Methionine; F (Phe) for Phenylalanine; W (Trp) for Tryptophan; K (Lys) for Lysine; Q (Gln) for Glutamine; E (Glu) for Glutamic Acid; S (Ser) for Serine; P (Pro) for Proline; V (Val) for Valine; I (Ile) for Isoleucine; C (Cys) for Cysteine; Y (Tyr) for Tyrosine; H (His) for Histidine; R (Arg) for Arginine; N (Asn) for Asparagine; D (Asp) for Aspartic Acid; T (Thr) for Threonine; B (Asx) for Aspartic acid or Asparagine; J (Xle) for Leucine or Isoleucine; O (Pyl) for Pyrrolysine; U (Sec) for Selenocysteine; X (Xaa) for any amino acid; and Z (Glx) for Glutamine or Glutamic acid.

Also provided herein are polynucleotide sequences encoding any of the AAV capsid variants described above and AAV particles, vectors, and cells comprising the same.

AAV Serotypes and Capsids

In some embodiments, an AAV particle of the present disclosure may comprise or be derived from any natural or recombinant AAV serotype. AAV serotypes may differ in characteristics such as, but not limited to, packaging, tropism, transduction and immunogenic profiles. While not wishing to be bound by theory, it is believed in some embodiments, that the AAV capsid protein, e.g., an AAV capsid variant, can modulate, e.g., direct, AAV particle tropism to a particular tissue.

In some embodiments, an AAV particle may have a capsid protein, e.g., an AAV capsid variant, and ITR sequences derived from the same parent serotype (e.g., AAV2 capsid and AAV2 ITRs). In another embodiment, the AAV particle may be a pseudo-typed AAV particle, wherein the capsid protein and ITR sequences are derived from different parent serotypes (e.g., AAV9 capsid and AAV2 ITRs; AAV2/9).

The AAV particles of the present disclosure may comprise an AAV capsid protein, e.g., an AAV capsid variant, with a peptide, e.g., targeting peptide, inserted into the parent sequence. The parent capsid or serotype may comprise or be derived from any natural or recombinant AAV serotype. As used herein, a “parent” sequence is a nucleotide or amino acid sequence into which a targeting sequence is inserted (e.g., a nucleotide insertion into nucleic acid sequence or amino acid sequence insertion into amino acid sequence).

In certain embodiments, the parent AAV capsid nucleotide sequence, e.g., a parental nucleic acid sequence, is as set forth in SEQ ID NO: 137. In some embodiments, the parent AAV capsid nucleotide sequence comprises the nucleic acid sequence of SEQ ID NO: 137, or a nucleic acid sequence substantially identical (e.g., having at least about 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence identity) at least thereto. In some embodiments, the parent AAV capsid amino acid sequence, e.g., the parental sequence, is encoded by the nucleic acid sequence of SEQ ID NO: 137, or a nucleic acid sequence substantially identical (e.g., having at least about 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence identity) at least thereto.

In some embodiments, the parent AAV capsid nucleotide sequence is a K449R variant of SEQ ID NO: 137, wherein the codon encoding a lysine (e.g., AAA or AAG) at position 449 in the amino acid sequence (nucleotides 1345-1347) is exchanged for one encoding an arginine (CGT, CGC, CGA, CGG, AGA, AGG). In some embodiments, the K449R variant has the same function as wild-type AAV9.

In some embodiments, the parent AAV capsid amino acid sequence, e.g., a parental amino acid sequence, is as set forth in SEQ ID NO: 138.

(SEQ ID NO: 138) MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPGY KYLGPGNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADAEF QERLKEDTSFGGNLGRAVFQAKKRLLEPLGLVEEAAKTAPGKKRPVEQSP QEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPSGVGS LTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVITTSTRTWALP TYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFSPRDWQR LINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTSTVQVFTDSDY QLPYVLGSAHEGCLPPFPADVFMIPQYGYLTLNDGSQAVGRSSFYCLEYF PSQMLRTGNNFQFSYEFENVPFHSSYAHSQSLDRLMNPLIDQYLYYLSKT INGSGQNQQTLKFSVAGPSNMAVQGRNYIPGPSYRQQRVSTTVTQNNNSE FAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSGSLIFGKQGTGR DNVDADKVMITNEEEIKTTNPVATESYGQVATNHQSAQAQAQTGWVQNQG ILPGMVWQDRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGMKHPPPQILIK NTPVPADPPTAFNKDKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQ YTSNYYKSNNVEFAVNTEGVYSEPRPIGTRYLTRNL

In some embodiments, the parental sequence comprises an amino acid sequence comprising at least one, two, or three modifications but no more than 30, 20, or 10 modifications, e.g., substitutions, relative to the amino acid sequence of SEQ ID NO: 138. In some embodiments, the parental sequence comprises the amino acid sequence of SEQ ID NO: 138, or an amino acid sequence substantially identical (e.g., having at least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence identity) thereto. In some embodiments, the parental sequence comprises substitution at position K449, e.g., a K449R substitution.

In some embodiments, the parent AAV capsid amino acid sequence, e.g., a parental amino acid sequence, is as set forth in SEQ ID NO: 11. In some embodiments, the parental sequence comprises an amino acid sequence comprising at least one, two, or three modifications but no more than 30, 20, or 10 modifications, e.g., substitutions, relative to the amino acid sequence of SEQ ID NO: 11. In some embodiments, the parental sequence comprises the amino acid sequence of SEQ ID NO: 11 or an amino acid sequence substantially identical (e.g., having at least 70%, 75%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, or 99% sequence identity) thereto.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be as described in Jackson et al (Frontiers in Molecular Neuroscience 9:154 (2016)), the contents of which are herein incorporated by reference in their entirety. In some embodiments, the AAV serotype of a parent AAV capsid described herein is PHP.B or AAV9. In some embodiments, the AAV serotype of a parent AAV capsid or AAV capsid variant is paired with a synapsin promoter to enhance neuronal transduction, as compared to when more ubiquitous promoters are used (e.g., CBA or CMV).

In some embodiments the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid is AAV9, or a variant thereof. In some embodiments, the AAV9 capsid comprises the amino acid sequence SEQ ID NO: 138. In some embodiments, the AAV9 amino acid sequence is encoded by a nucleotide sequence comprising SEQ ID NO: 137. In some embodiments, the AAV9 capsid comprises an amino acid sequence at least 70% identical to SEQ ID NO: 138, such as, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or greater than 99%. In some embodiments, the AAV9 capsid comprises a nucleotide sequence at least 70% identical to SEQ ID NO: 137, such as, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or greater than 99%.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid is AAV9 K449R, or a variant thereof. In some embodiments, the AAV9 K449R capsid comprises the amino acid sequence SEQ ID NO: 11. In some embodiments, the AAV9 K449R capsid comprises an amino acid sequence at least 70% identical to SEQ ID NO: 11, such as, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or greater than 99%.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid comprises an AAVDJ sequence. In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid comprises an AAVDJ8 sequence. In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid comprises an AAVrh10 sequence. In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid comprises an AAV1 sequence. In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid comprises AAVF7/HSC7 (SEQ ID NO: 8 and 27 of WO2016049230). In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid comprises AAVF15/HSC15 (SEQ ID NO: 16 and 33 of WO2016049230). In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid comprises AAVF17/HSC17 (SEQ ID NO: 13 and 35 of WO2016049230). In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid comprises an AAV5 sequence. As a non-limiting example, the AAV5 sequence is SEQ ID NO: 4 of U.S. Pat. No. 6,984,517, the contents of which are herein incorporated by reference in their entirety.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, allows for blood brain barrier penetration following intravenous administration. In some embodiments, the AAV capsid, e.g., AAV capsid variant, allows for blood brain barrier penetration following focused ultrasound (FUS), e.g., coupled with the intravenous administration of microbubbles (FUS-MB), or MRI-guided FUS coupled with intravenous administration. In some embodiments the AAV capsid, e.g., AAV capsid variant allows for increased distribution to a brain region. In some embodiments, the brain region comprises a frontal cortex, sensory cortex, motor cortex, caudate, dentate nucleus, cerebellar cortex, cerebral cortex, brain stem, hippocampus, thalamus, putamen, or a combination thereof. In some embodiments, the AAV capsid, e.g., AAV capsid variant allows for preferential transduction in a brain region relative to the transduction in the dorsal root ganglia (DRG).

In some embodiments the AAV capsid polypeptide, e.g., AAV capsid variant allows for increased distribution to a spinal cord region. In some embodiments, the spinal region comprises a cervical spinal cord region, thoracic spinal cord region, and/or lumbar spinal cord region.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, is suitable for intramuscular administration and/or transduction of muscle fibers. In some embodiments the AAV capsid polypeptide, e.g., AAV capsid variant, allows for increased distribution to a muscle region. In some embodiments, the muscle region comprises a heart muscle, quadriceps muscle, a diaphragm muscle region, or a combination thereof. In some embodiments, the muscle region comprises a heart muscle region, e.g., a heart atrium muscle region or a heart ventricle muscle region.

In some embodiments, one or more targeting sequence is inserted into a parent AAV capsid sequence or an AAV capsid polypeptide, e.g., AAV capsid variant, sequence. As a non-limiting example, one targeting sequence may be inserted into a parent AAV capsid sequence or an AAV capsid variant sequence. As a non-limiting example, two targeting sequences may be inserted into a parent AAV capsid sequence or an AAV capsid variant sequence. As a non-limiting example, three targeting sequences may be inserted into a parent AAV capsid sequence or an AAV capsid variant sequence. As a non-limiting example, more than three targeting sequences may be inserted into a parent AAV capsid sequence or an AAV capsid variant sequence.

In some embodiments, an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein comprises a parental AAV capsid sequence having an insert, e.g., an amino acid sequence as described in Table 1 or 2. In some embodiments, the parental sequence may comprise at least 1, 2, 3 or more insert sequences.

While not wishing to be bound by theory, it is understood that a parent AAV capsid sequence or an AAV capsid polypeptide, e.g., an AAV capsid variant, comprises a VP1 region. In some embodiments, a parent AAV capsid sequence or an AAV capsid variant comprises a VP1, VP2 and/or VP3 region, or any combination thereof.

In some embodiments, the initiation codon for translation of the AAV VP1 capsid protein, e.g., a capsid variant, may be CTG, TTG, or GTG as described in U.S. Pat. No. 8,163,543, the contents of which are herein incorporated by reference in its entirety.

The present disclosure refers to structural capsid proteins (including VP1, VP2 and VP3) which are encoded by capsid (Cap) genes. These capsid proteins form an outer protein structural shell (e.g. capsid) of a viral vector such as AAV. VP capsid proteins synthesized from Cap polynucleotides generally include a methionine as the first amino acid in the peptide sequence (Met1), which is associated with the start codon (AUG or ATG) in the corresponding Cap nucleotide sequence. However, it is common for a first-methionine (Met1) residue or generally any first amino acid (AA1) to be cleaved off after or during polypeptide synthesis by protein processing enzymes such as Met-aminopeptidases. This “Met/AA-clipping” process often correlates with a corresponding acetylation of the second amino acid in the polypeptide sequence (e.g., alanine, valine, serine, threonine, etc.). Met-clipping commonly occurs with VP1 and VP3 capsid proteins but can also occur with VP2 capsid proteins.

Where the Met/AA-clipping is incomplete, a mixture of one or more (one, two or three) VP capsid proteins comprising the viral capsid may be produced, some of which may include a Met1/AA1 amino acid (Met+/AA+) and some of which may lack a Met1/AA1 amino acid as a result of Met/AA-clipping (Met−/AA−). For further discussion regarding Met/AA-clipping in capsid proteins, see Jin, et al. Direct Liquid Chromatography/Mass Spectrometry Analysis for Complete Characterization of Recombinant Adeno-Associated Virus Capsid Proteins. Hum Gene Ther Methods. 2017 Oct. 28(5):255-267; Hwang, et al. N-Terminal Acetylation of Cellular Proteins Creates Specific Degradation Signals. Science. 2010 Feb. 19. 327(5968): 973-977; the contents of which are each incorporated herein by reference in its entirety.

According to the present disclosure, references to capsid proteins, e.g., AAV capsid variants, is not limited to either clipped (Met−/AA−) or unclipped (Met+/AA+) and may, in context, refer to independent capsid proteins, viral capsids comprised of a mixture of capsid proteins, and/or polynucleotide sequences (or fragments thereof) which encode, describe, produce or result in capsid proteins of the present disclosure. A direct reference to a capsid protein or capsid polypeptide (such as VP1, VP2 or VP2) may also comprise VP capsid proteins which include a Met1/AA1 amino acid (Met+/AA+) as well as corresponding VP capsid proteins which lack the Met1/AA1 amino acid as a result of Met/AA-clipping (Met−/AA−).

Further according to the present disclosure, a reference to a specific SEQ ID NO: (whether a protein or nucleic acid) which comprises or encodes, respectively, one or more capsid proteins which include a Met1/AA1 amino acid (Met+/AA+) should be understood to teach the VP capsid proteins which lack the Met1/AA1 amino acid as upon review of the sequence, it is readily apparent any sequence which merely lacks the first listed amino acid (whether or not Met1/AA1).

As a non-limiting example, reference to a VP1 polypeptide sequence which is 736 amino acids in length and which includes a “Met1” amino acid (Met+) encoded by the AUG/ATG start codon may also be understood to teach a VP1 polypeptide sequence which is 735 amino acids in length and which does not include the “Met1” amino acid (Met−) of the 736 amino acid Met+ sequence. As a second non-limiting example, reference to a VP1 polypeptide sequence which is 736 amino acids in length and which includes an “AA1” amino acid (AA1+) encoded by any NNN initiator codon may also be understood to teach a VP1 polypeptide sequence which is 735 amino acids in length and which does not include the “AA1” amino acid (AA1−) of the 736 amino acid AA1+ sequence.

References to viral capsids formed from VP capsid proteins (such as reference to specific AAV capsid serotypes), can incorporate VP capsid proteins which include a Met1/AA1 amino acid (Met+/AA1+), corresponding VP capsid proteins which lack the Met1/AA1 amino acid as a result of Met/AA1-clipping (Met−/AA1−), and combinations thereof (Met+/AA1+ and Met−/AA1−).

As a non-limiting example, an AAV capsid serotype can include VP1 (Met+/AA1+), VP1 (Met−/AA1−), or a combination of VP1 (Met+/AA1+) and VP1 (Met−/AA1−). An AAV capsid serotype can also include VP3 (Met+/AA1+), VP3 (Met−/AA1−), or a combination of VP3 (Met+/AA1+) and VP3 (Met−/AA1−); and can also include similar optional combinations of VP2 (Met+/AA1) and VP2 (Met−/AA1−).

Additional AAV Sequences

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, comprises, or the parent AAV capsid is modified such that, immediately subsequent to position 586, 588, or 589 numbered relative to SEQ ID NO: 138, the AAV capsid variant or the parent AAV capsid polypeptide comprises at least 3, 4, 5, 6, 7, 8, or 9 consecutive amino acids of any of SEQ ID NOs: 1725-3622 or 3648-3659.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid described herein does not comprise an insert sequence present immediately subsequent to position 586, 588, or 589 numbered relative to SEQ ID NO: 138, having at least 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, of any of SEQ ID NOs: 1-1724, e.g., as described in Table 6.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may comprise at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, an amino acid sequence with 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identity to any of those described herein.

In any of the embodiments described herein, a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138 can be identified by providing an alignment of a reference sequence and a query sequence, wherein the reference sequence is SEQ ID NO: 138, and identifying the residues corresponding to the positions in the query sequence that correspond to positions 586 to 594 in the reference sequence.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be encoded by a nucleic acid sequence with 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identity to any of those described herein, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be an AAV9, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138. In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, an AAV9hu.14 (SEQ ID NO: 137 or 138). In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, an AAV9 K449R (SEQ ID NO: 11). In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a PHP.B (SEQ ID NO: 5 or 6). In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a PHP.N (SEQ ID NO: 4). In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a VOY101 (SEQ ID NO: 1 or 2). In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a VOY201 (SEQ ID NO: 3 or 1724).

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, an AAV5.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, selected from any of the following VOY101, VOY201, AAVPHP.B (PHP.B), AAVPHP.A (PHP.A), AAVG2B-26, AAVG2B-13, AAVTH1.1-32, AAVTH1.1-35, AAVPHP.B2 (PHP.B2), AAVPHP.B3 (PHP.B3), AAVPHP.N/PHP.B-DGT, AAVPHP.B-EST, AAVPHP.B-GGT, AAVPHP.B-ATP, AAVPHP.B-ATT-T, AAVPHP.B-DGT-T, AAVPHP.B-GGT-T, AAVPHP.B-SGS, AAVPHP.B-AQP, AAVPHP.B-QQP, AAVPHP.B-SNP(3), AAVPHP.B-SNP, AAVPHP.B-QGT, AAVPHP.B-NQT, AAVPHP.B-EGS, AAVPHP.B-SGN, AAVPHP.B-EGT, AAVPHP.B-DST, AAVPHP.B-DST, AAVPHP.B-STP, AAVPHP.B-PQP, AAVPHP.B-SQP, AAVPHP.B-QLP, AAVPHP.B-TMP, AAVPHP.B-TTP, AAVPHP.S/G2A12, AAVG2A15/G2A3 (G2A3), AAVG2B4 (G2B4), AAVG2B5 (G2B5), PHP.S, AAV1, AAV2, AAV2G9, AAV3, AAV3a, AAV3b, AAV3-3, AAV4, AAV4-4, AAV5, AAV6, AAV6.1, AAV6.2, AAV6.1.2, AAV7, AAV7.2, AAV8, AAV9, AAV9 K449R, AAV9.11, AAV9.13, AAV9.16, AAV9.24, AAV9.45, AAV9.47, AAV9.61, AAV9.68, AAV9.84, AAV9.9, AAV10, AAV11, AAV12, AAV16.3, AAV24.1, AAV27.3, AAV42.12, AAV42-1b, AAV42-2, AAV42-3a, AAV42-3b, AAV42-4, AAV42-5a, AAV42-5b, AAV42-6b, AAV42-8, AAV42-10, AAV42-11, AAV42-12, AAV42-13, AAV42-15, AAV42-aa, AAV43-1, AAV43-12, AAV43-20, AAV43-21, AAV43-23, AAV43-25, AAV43-5, AAV44.1, AAV44.2, AAV44.5, AAV223.1, AAV223.2, AAV223.4, AAV223.5, AAV223.6, AAV223.7, AAV1-7/rh.48, AAV1-8/rh.49, AAV2-15/rh.62, AAV2-3/rh.61, AAV2-4/rh.50, AAV2-5/rh.51, AAV3.1/hu.6, AAV3.1/hu.9, AAV3-9/rh.52, AAV3-11/rh.53, AAV4-8/r11.64, AAV4-9/rh.54, AAV4-19/rh.55, AAV5-3/rh.57, AAV5-22/rh.58, AAV7.3/hu.7, AAV16.8/hu.10, AAV16.12/hu.011, AAV29.3/bb.1, AAV29.5/bb.2, AAV106.1/hu.37, AAV114.3/hu.40, AAV127.2/hu.41, AAV127.5/hu.42, AAV128.3/hu.44, AAV130.4/hu.48, AAV145.1/hu.53, AAV145.5/hu.54, AAV145.6/hu.55, AAV161.10/hu.60, AAV161.6/hu.61, AAV33.12/hu.17, AAV33.4/hu.15, AAV33.8/hu.16, AAV52/hu.19, AAV52.1/hu.20, AAV58.2/hu.25, AAVA3.3, AAVA3.4, AAVA3.5, AAVA3.7, AAVC1, AAVC2, AAVC5, AAV-DJ, AAV-DJ8, AAVF3, AAVF5, AAVH2, AAVrh.72, AAVhu.8, AAVrh.68, AAVrh.70, AAVpi.1, AAVpi.3, AAVpi.2, AAVrh.60, AAVrh.44, AAVrh.65, AAVrh.55, AAVrh.47, AAVrh.69, AAVrh.45, AAVrh.59, AAVhu.12, AAVH6, AAVLK03, AAVH-1/hu.1, AAVH-5/hu.3, AAVLG-10/rh.40, AAVLG-4/rh.38, AAVLG-9/hu.39, AAVN721-8/rh.43, AAVCh.5, AAVCh.5R1, AAVcy.2, AAVcy.3, AAVcy.4, AAVcy.5, AAVCy.5R1, AAVCy.5R2, AAVCy.5R3, AAVCy.5R4, AAVcy.6, AAVhu.1, AAVhu.2, AAVhu.3, AAVhu.4, AAVhu.5, AAVhu.6, AAVhu.7, AAVhu.9, AAVhu.10, AAVhu.11, AAVhu.13, AAVhu.15, AAVhu.16, AAVhu.17, AAVhu.18, AAVhu.20, AAVhu.21, AAVhu.22, AAVhu.23.2, AAVhu.24, AAVhu.25, AAVhu.27, AAVhu.28, AAVhu.29, AAVhu.29R, AAVhu.31, AAVhu.32, AAVhu.34, AAVhu.35, AAVhu.37, AAVhu.39, AAVhu.40, AAVhu.41, AAVhu.42, AAVhu.43, AAVhu.44, AAVhu.44R1, AAVhu.44R2, AAVhu.44R3, AAVhu.45, AAVhu.46, AAVhu.47, AAVhu.48, AAVhu.48R1, AAVhu.48R2, AAVhu.48R3, AAVhu.49, AAVhu.51, AAVhu.52, AAVhu.54, AAVhu.55, AAVhu.56, AAVhu.57, AAVhu.58, AAVhu.60, AAVhu.61, AAVhu.63, AAVhu.64, AAVhu.66, AAVhu.67, AAVhu.14/9, AAVhu.t 19, AAVrh.2, AAVrh.2R, AAVrh.8, AAVrh.8R, AAVrh.10, AAVrh.12, AAVrh.13, AAVrh.13R, AAVrh.14, AAVrh.17, AAVrh.18, AAVrh.19, AAVrh.20, AAVrh.21, AAVrh.22, AAVrh.23, AAVrh.24, AAVrh.25, AAVrh.31, AAVrh.32, AAVrh.33, AAVrh.34, AAVrh.35, AAVrh.36, AAVrh.37, AAVrh.37R2, AAVrh.38, AAVrh.39, AAVrh.40, AAVrh.46, AAVrh.48, AAVrh.48.1, AAVrh.48.1.2, AAVrh.48.2, AAVrh.49, AAVrh.51, AAVrh.52, AAVrh.53, AAVrh.54, AAVrh.56, AAVrh.57, AAVrh.58, AAVrh.61, AAVrh.64, AAVrh.64R1, AAVrh.64R2, AAVrh.67, AAVrh.73, AAVrh.74, AAVrh8R, AAVrh8R A586R mutant, AAVrh8R R533A mutant, AAAV, BAAV, caprine AAV, bovine AAV, AAVhE1.1, AAVhEr1.5, AAVhER1.14, AAVhEr1.8, AAVhEr1.16, AAVhEr1.18, AAVhEr1.35, AAVhEr1.7, AAVhEr1.36, AAVhEr2.29, AAVhEr2.4, AAVhEr2.16, AAVhEr2.30, AAVhEr2.31, AAVhEr2.36, AAVhER1.23, AAVhEr3.1, AAV2.5T, AAV-PAEC, AAV-LK01, AAV-LK02, AAV-LK03, AAV-LK04, AAV-LK05, AAV-LK06, AAV-LK07, AAV-LK08, AAV-LK09, AAV-LK10, AAV-LK11, AAV-LK12, AAV-LK13, AAV-LK14, AAV-LK15, AAV-LK16, AAV-LK17, AAV-LK18, AAV-LK19, AAV-PAEC2, AAV-PAEC4, AAV-PAEC6, AAV-PAEC7, AAV-PAEC8, AAV-PAEC11, AAV-PAEC12, AAV-2-pre-miRNA-101, AAV-8h, AAV-8b, AAV-h, AAV-b, AAV SM 10-2, AAV Shuffle 100-1, AAV Shuffle 100-3, AAV Shuffle 100-7, AAV Shuffle 10-2, AAV Shuffle 10-6, AAV Shuffle 10-8, AAV Shuffle 100-2, AAV SM 10-1, AAV SM 10-8, AAV SM 100-3, AAV SM 100-10, BNP61 AAV, BNP62 AAV, BNP63 AAV, AAVrh.50, AAVrh.43, AAVrh.62, AAVrh.48, AAVhu.19, AAVhu.11, AAVhu.53, AAV4-8/rh.64, AAVLG-9/hu.39, AAV54.5/hu.23, AAV54.2/hu.22, AAV54.7/hu.24, AAV54.1/hu.21, AAV54.4R/hu.27, AAV46.2/hu.28, AAV46.6/hu.29, AAV128.1/hu.43, true type AAV (ttAAV), UPENN AAV 10, Japanese AAV 10 serotypes, AAV CBr-7.1, AAV CBr-7.10, AAV CBr-7.2, AAV CBr-7.3, AAV CBr-7.4, AAV CBr-7.5, AAV CBr-7.7, AAV CBr-7.8, AAV CBr-B7.3, AAV CBr-B7.4, AAV CBr-E1, AAV CBr-E2, AAV CBr-E3, AAV CBr-E4, AAV CBr-E5, AAV CBr-e5, AAV CBr-E6, AAV CBr-E7, AAV CBr-E8, AAV CHt-1, AAV CHt-2, AAV CHt-3, AAV CHt-6.1, AAV CHt-6.10, AAV CHt-6.5, AAV CHt-6.6, AAV CHt-6.7, AAV CHt-6.8, AAV CHt-P1, AAV CHt-P2, AAV CHt-P5, AAV CHt-P6, AAV CHt-P8, AAV CHt-P9, AAV CKd-1, AAV CKd-10, AAV CKd-2, AAV CKd-3, AAV CKd-4, AAV CKd-6, AAV CKd-7, AAV CKd-8, AAV CKd-B1, AAV CKd-B2, AAV CKd-B3, AAV CKd-B4, AAV CKd-B5, AAV CKd-B6, AAV CKd-B7, AAV CKd-B8, AAV CKd-H1, AAV CKd-H2, AAV CKd-H3, AAV CKd-H4, AAV CKd-H5, AAV CKd-H6, AAV CKd-N3, AAV CKd-N4, AAV CKd-N9, AAV CLg-F1, AAV CLg-F2, AAV CLg-F3, AAV CLg-F4, AAV CLg-F5, AAV CLg-F6, AAV CLg-F7, AAV CLg-F8, AAV CLv-1, AAV CLv1-1, AAV Clv1-10, AAV CLv1-2, AAV CLv-12, AAV CLv1-3, AAV CLv-13, AAV CLv1-4, AAV Clv1-7, AAV Clv1-8, AAV Clv1-9, AAV CLv-2, AAV CLv-3, AAV CLv-4, AAV CLv-6, AAV CLv-8, AAV CLv-D1, AAV CLv-D2, AAV CLv-D3, AAV CLv-D4, AAV CLv-D5, AAV CLv-D6, AAV CLv-D7, AAV CLv-D8, AAV CLv-E1, AAV CLv-K1, AAV CLv-K3, AAV CLv-K6, AAV CLv-L4, AAV CLv-L5, AAV CLv-L6, AAV CLv-M1, AAV CLv-M11, AAV CLv-M2, AAV CLv-M5, AAV CLv-M6, AAV CLv-M7, AAV CLv-M8, AAV CLv-M9, AAV CLv-R1, AAV CLv-R2, AAV CLv-R3, AAV CLv-R4, AAV CLv-R5, AAV CLv-R6, AAV CLv-R7, AAV CLv-R8, AAV CLv-R9, AAV CSp-1, AAV CSp-10, AAV CSp-11, AAV CSp-2, AAV CSp-3, AAV CSp-4, AAV CSp-6, AAV CSp-7, AAV CSp-8, AAV CSp-8.10, AAV CSp-8.2, AAV CSp-8.4, AAV CSp-8.5, AAV CSp-8.6, AAV CSp-8.7, AAV CSp-8.8, AAV CSp-8.9, AAV CSp-9, AAV.hu.48R3, AAV.VR-355, AAV3B, AAV4, AAV5, AAVF1/HSC1, AAVF11/HSC11, AAVF12/HSC12, AAVF13/HSC13, AAVF14/HSC14, AAVF15/HSC15, AAVF16/HSC16, AAVF17/HSC17, AAVF2/HSC2, AAVF3/HSC3, AAVF4/HSC4, AAVF5/HSC5, AAVF6/HSC6, AAVF7/HSC7, AAVF8/HSC8, and/or AAVF9/HSC9 and variants thereof.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence as described in United States Publication No. US20030138772, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAV1 (SEQ ID NO: 6 and 64 of US20030138772), AAV2 (SEQ ID NO: 7 and 70 of US20030138772), AAV3 (SEQ ID NO: 8 and 71 of US20030138772), AAV4 (SEQ ID NO: 63 of US20030138772), AAV5 (SEQ ID NO: 114 of US20030138772), AAV6 (SEQ ID NO: 65 of US20030138772), AAV7 (SEQ ID NO: 1-3 of US20030138772), AAV8 (SEQ ID NO: 4 and 95 of US20030138772), AAV9 (SEQ ID NO: 5 and 100 of US20030138772), AAV10 (SEQ ID NO: 117 of US20030138772), AAV11 (SEQ ID NO: 118 of US20030138772), AAV12 (SEQ ID NO: 119 of US20030138772), AAVrh10 (amino acids 1 to 738 of SEQ ID NO: 81 of US20030138772), AAV16.3 (US20030138772 SEQ ID NO: 10), AAV29.3/bb.1 (US20030138772 SEQ ID NO: 11), AAV29.4 (US20030138772 SEQ ID NO: 12), AAV29.5/bb.2 (US20030138772 SEQ ID NO: 13), AAV1.3 (US20030138772 SEQ ID NO: 14), AAV13.3 (US20030138772 SEQ ID NO: 15), AAV24.1 (US20030138772 SEQ ID NO: 16), AAV27.3 (US20030138772 SEQ ID NO: 17), AAV7.2 (US20030138772 SEQ ID NO: 18), AAVC1 (US20030138772 SEQ ID NO: 19), AAVC3 (US20030138772 SEQ ID NO: 20), AAVC5 (US20030138772 SEQ ID NO: 21), AAVF1 (US20030138772 SEQ ID NO: 22), AAVF3 (US20030138772 SEQ ID NO: 23), AAVF5 (US20030138772 SEQ ID NO: 24), AAVH6 (US20030138772 SEQ ID NO: 25), AAVH2 (US20030138772 SEQ ID NO: 26), AAV42-8 (US20030138772 SEQ ID NO: 27), AAV42-15 (US20030138772 SEQ ID NO: 28), AAV42-5b (US20030138772 SEQ ID NO: 29), AAV42-1b (US20030138772 SEQ ID NO: 30), AAV42-13 (US20030138772 SEQ ID NO: 31), AAV42-3a (US20030138772 SEQ ID NO: 32), AAV42-4 (US20030138772 SEQ ID NO: 33), AAV42-5a (US20030138772 SEQ ID NO: 34), AAV42-10 (US20030138772 SEQ ID NO: 35), AAV42-3b (US20030138772 SEQ ID NO: 36), AAV42-11 (US20030138772 SEQ ID NO: 37), AAV42-6b (US20030138772 SEQ ID NO: 38), AAV43-1 (US20030138772 SEQ ID NO: 39), AAV43-5 (US20030138772 SEQ ID NO: 40), AAV43-12 (US20030138772 SEQ ID NO: 41), AAV43-20 (US20030138772 SEQ ID NO: 42), AAV43-21 (US20030138772 SEQ ID NO: 43), AAV43-23 (US20030138772 SEQ ID NO: 44), AAV43-25 (US20030138772 SEQ ID NO: 45), AAV44.1 (US20030138772 SEQ ID NO: 46), AAV44.5 (US20030138772 SEQ ID NO: 47), AAV223.1 (US20030138772 SEQ ID NO: 48), AAV223.2 (US20030138772 SEQ ID NO: 49), AAV223.4 (US20030138772 SEQ ID NO: 50), AAV223.5 (US20030138772 SEQ ID NO: 51), AAV223.6 (US20030138772 SEQ ID NO: 52), AAV223.7 (US20030138772 SEQ ID NO: 53), AAVA3.4 (US20030138772 SEQ ID NO: 54), AAVA3.5 (US20030138772 SEQ ID NO: 55), AAVA3.7 (US20030138772 SEQ ID NO: 56), AAVA3.3 (US20030138772 SEQ ID NO: 57), AAV42.12 (US20030138772 SEQ ID NO: 58), AAV44.2 (US20030138772 SEQ ID NO: 59), AAV42-2 (US20030138772 SEQ ID NO: 9), or variants thereof.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence as described in United States Publication No. US20150159173, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAV2 (SEQ ID NO: 7 and 23 of US20150159173), rh20 (SEQ ID NO: 1 of US20150159173), rh32/33 (SEQ ID NO: 2 of US20150159173), rh39 (SEQ ID NO: 3, 20 and 36 of US20150159173), rh46 (SEQ ID NO: 4 and 22 of US20150159173), rh73 (SEQ ID NO: 5 of US20150159173), rh74 (SEQ ID NO: 6 of US20150159173), AAV6.1 (SEQ ID NO: 29 of US20150159173), rh.8 (SEQ ID NO: 41 of US20150159173), rh.48.1 (SEQ ID NO: 44 of US20150159173), hu.44 (SEQ ID NO: 45 of US20150159173), hu.29 (SEQ ID NO: 42 of US20150159173), hu.48 (SEQ ID NO: 38 of US20150159173), rh54 (SEQ ID NO: 49 of US20150159173), AAV2 (SEQ ID NO: 7 of US20150159173), cy.5 (SEQ ID NO: 8 and 24 of US20150159173), rh.10 (SEQ ID NO: 9 and 25 of US20150159173), rh.13 (SEQ ID NO: 10 and 26 of US20150159173), AAV1 (SEQ ID NO: 11 and 27 of US20150159173), AAV3 (SEQ ID NO: 12 and 28 of US20150159173), AAV6 (SEQ ID NO: 13 and 29 of US20150159173), AAV7 (SEQ ID NO: 14 and 30 of US20150159173), AAV8 (SEQ ID NO: 15 and 31 of US20150159173), hu.13 (SEQ ID NO: 16 and 32 of US20150159173), hu.26 (SEQ ID NO: 17 and 33 of US20150159173), hu.37 (SEQ ID NO: 18 and 34 of US20150159173), hu.53 (SEQ ID NO: 19 and 35 of US20150159173), rh.43 (SEQ ID NO: 21 and 37 of US20150159173), rh2 (SEQ ID NO: 39 of US20150159173), rh.37 (SEQ ID NO: 40 of US20150159173), rh.64 (SEQ ID NO: 43 of US20150159173), rh.48 (SEQ ID NO: 44 of US20150159173), ch.5 (SEQ ID NO 46 of US20150159173), rh.67 (SEQ ID NO: 47 of US20150159173), rh.58 (SEQ ID NO: 48 of US20150159173), or variants thereof including, but not limited to Cy5R1, Cy5R2, Cy5R3, Cy5R4, rh.13R, rh.37R2, rh.2R, rh.8R, rh.48.1, rh.48.2, rh.48.1.2, hu.44R1, hu.44R2, hu.44R3, hu.29R, ch.5R1, rh64R1, rh64R2, AAV6.2, AAV6.1, AAV6.12, hu.48R1, hu.48R2, and hu.48R3.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence as described in U.S. Pat. No. 7,198,951, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAV9 (SEQ ID NO: 1-3 of U.S. Pat. No. 7,198,951), AAV2 (SEQ ID NO: 4 of U.S. Pat. No. 7,198,951), AAV1 (SEQ ID NO: 5 of U.S. Pat. No. 7,198,951), AAV3 (SEQ ID NO: 6 of U.S. Pat. No. 7,198,951), and AAV8 (SEQ ID NO: 7 of U.S. Pat. No. 7,198,951).

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a mutation in the AAV9 sequence as described by N Pulicherla et al. (Molecular Therapy 19(6):1070-1078 (2011), herein incorporated by reference in its entirety), such as but not limited to, AAV9.9, AAV9.11, AAV9.13, AAV9.16, AAV9.24, AAV9.45, AAV9.47, AAV9.61, AAV9.68, AAV9.84.

In some embodiments, the AAV serotype, the parent AAV capsid polypeptide, or the AAV capsid variant may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence as described in U.S. Pat. No. 6,156,303, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAV3B (SEQ ID NO: 1 and 10 of U.S. Pat. No. 6,156,303), AAV6 (SEQ ID NO: 2, 7 and 11 of U.S. Pat. No. 6,156,303), AAV2 (SEQ ID NO: 3 and 8 of U.S. Pat. No. 6,156,303), AAV3A (SEQ ID NO: 4 and 9, of U.S. Pat. No. 6,156,303), or derivatives thereof.

In some embodiments, the AAV serotype, the parent AAV capsid polypeptide, or the AAV capsid variant may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence as described in United States Publication No. US20140359799, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAV8 (SEQ ID NO: 1 of US20140359799), AAVDJ (SEQ ID NO: 2 and 3 of US20140359799), or variants thereof.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a AAVDJ or a variant thereof, such as AAVDJ8 (or AAV-DJ8), as described by Grimm et al. (Journal of Virology 82(12): 5887-5911 (2008), herein incorporated by reference in its entirety). In some embodiments, the amino acid sequence of AAVDJ8 may comprise two or more mutations in order to remove the heparin binding domain (HBD). In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may comprise, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, the AAV-DJ sequence described as SEQ ID NO: 1 in U.S. Pat. No. 7,588,772, the contents of which are herein incorporated by reference in their entirety.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence of AAV4 as described in International Publication No. WO1998011244, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to AAV4 (SEQ ID NO: 1-20 of WO1998011244).

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a mutation in the AAV2 sequence to generate AAV2G9 as described in International Publication No. WO2014144229 and herein incorporated by reference in its entirety.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence as described in International Publication No. WO2005033321, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to AAV3-3 (SEQ ID NO: 217 of WO2005033321), AAV1 (SEQ ID NO: 219 and 202 of WO2005033321), AAV106.1/hu.37 (SEQ ID No: 10 of WO2005033321), AAV114.3/hu.40 (SEQ ID No: 11 of WO2005033321), AAV127.2/hu.41 (SEQ ID NO:6 and 8 of WO2005033321), AAV128.3/hu.44 (SEQ ID No: 81 of WO2005033321), AAV130.4/hu.48 (SEQ ID NO: 78 of WO2005033321), AAV145.1/hu.53 (SEQ ID No: 176 and 177 of WO2005033321), AAV145.6/hu.56 (SEQ ID NO: 168 and 192 of WO2005033321), AAV16.12/hu.11 (SEQ ID NO: 153 and 57 of WO2005033321), AAV16.8/hu.10 (SEQ ID NO: 156 and 56 of WO2005033321), AAV161.10/hu.60 (SEQ ID No: 170 of WO2005033321), AAV161.6/hu.61 (SEQ ID No: 174 of WO2005033321), AAV1-7/rh.48 (SEQ ID NO: 32 of WO2005033321), AAV1-8/rh.49 (SEQ ID NOs: 103 and 25 of WO2005033321), AAV2 (SEQ ID NO: 211 and 221 of WO2005033321), AAV2-15/rh.62 (SEQ ID No: 33 and 114 of WO2005033321), AAV2-3/rh.61 (SEQ ID NO: 21 of WO2005033321), AAV2-4/rh.50 (SEQ ID No: 23 and 108 of WO2005033321), AAV2-5/rh.51 (SEQ ID NO: 104 and 22 of WO2005033321), AAV3.1/hu.6 (SEQ ID NO: 5 and 84 of WO2005033321), AAV3.1/hu.9 (SEQ ID NO: 155 and 58 of WO2005033321), AAV3-11/rh.53 (SEQ ID NO: 186 and 176 of WO2005033321), AAV3-3 (SEQ ID NO: 200 of WO2005033321), AAV33.12/hu.17 (SEQ ID NO:4 of WO2005033321), AAV33.4/hu.15 (SEQ ID No: 50 of WO2005033321), AAV33.8/hu.16 (SEQ ID No: 51 of WO2005033321), AAV3-9/rh.52 (SEQ ID NO: 96 and 18 of WO2005033321), AAV4-19/rh.55 (SEQ ID NO: 117 of WO2005033321), AAV4-4 (SEQ ID NO: 201 and 218 of WO2005033321), AAV4-9/rh.54 (SEQ ID NO: 116 of WO2005033321), AAV5 (SEQ ID NO: 199 and 216 of WO2005033321), AAV52.1/hu.20 (SEQ ID NO: 63 of WO2005033321), AAV52/hu.19 (SEQ ID NO: 133 of WO2005033321), AAV5-22/rh.58 (SEQ ID No: 27 of WO2005033321), AAV5-3/rh.57 (SEQ ID NO: 105 of WO2005033321), AAV5-3/rh.57 (SEQ ID No: 26 of WO2005033321), AAV58.2/hu.25 (SEQ ID No: 49 of WO2005033321), AAV6 (SEQ ID NO: 203 and 220 of WO2005033321), AAV7 (SEQ ID NO: 222 and 213 of WO2005033321), AAV7.3/hu.7 (SEQ ID No: 55 of WO2005033321), AAV8 (SEQ ID NO: 223 and 214 of WO2005033321), AAVH-1/hu.1 (SEQ ID No: 46 of WO2005033321), AAVH-5/hu.3 (SEQ ID No: 44 of WO2005033321), AAVhu.1 (SEQ ID NO: 144 of WO2005033321), AAVhu.10 (SEQ ID NO: 156 of WO2005033321), AAVhu.11 (SEQ ID NO: 153 of WO2005033321), AAVhu.12 (WO2005033321 SEQ ID NO: 59), AAVhu.13 (SEQ ID NO: 129 of WO2005033321), AAVhu.14/AAV9 (SEQ ID NO: 123 and 3 of WO2005033321), AAVhu.15 (SEQ ID NO: 147 of WO2005033321), AAVhu.16 (SEQ ID NO: 148 of WO2005033321), AAVhu.17 (SEQ ID NO: 83 of WO2005033321), AAVhu.18 (SEQ ID NO: 149 of WO2005033321), AAVhu.19 (SEQ ID NO: 133 of WO2005033321), AAVhu.2 (SEQ ID NO: 143 of WO2005033321), AAVhu.20 (SEQ ID NO: 134 of WO2005033321), AAVhu.21 (SEQ ID NO: 135 of WO2005033321), AAVhu.22 (SEQ ID NO: 138 of WO2005033321), AAVhu.23.2 (SEQ ID NO: 137 of WO2005033321), AAVhu.24 (SEQ ID NO: 136 of WO2005033321), AAVhu.25 (SEQ ID NO: 146 of WO2005033321), AAVhu.27 (SEQ ID NO: 140 of WO2005033321), AAVhu.29 (SEQ ID NO: 132 of WO2005033321), AAVhu.3 (SEQ ID NO: 145 of WO2005033321), AAVhu.31 (SEQ ID NO: 121 of WO2005033321), AAVhu.32 (SEQ ID NO: 122 of WO2005033321), AAVhu.34 (SEQ ID NO: 125 of WO2005033321), AAVhu.35 (SEQ ID NO: 164 of WO2005033321), AAVhu.37 (SEQ ID NO: 88 of WO2005033321), AAVhu.39 (SEQ ID NO: 102 of WO2005033321), AAVhu.4 (SEQ ID NO: 141 of WO2005033321), AAVhu.40 (SEQ ID NO: 87 of WO2005033321), AAVhu.41 (SEQ ID NO: 91 of WO2005033321), AAVhu.42 (SEQ ID NO: 85 of WO2005033321), AAVhu.43 (SEQ ID NO: 160 of WO2005033321), AAVhu.44 (SEQ ID NO: 144 of WO2005033321), AAVhu.45 (SEQ ID NO: 127 of WO2005033321), AAVhu.46 (SEQ ID NO: 159 of WO2005033321), AAVhu.47 (SEQ ID NO: 128 of WO2005033321), AAVhu.48 (SEQ ID NO: 157 of WO2005033321), AAVhu.49 (SEQ ID NO: 189 of WO2005033321), AAVhu.51 (SEQ ID NO: 190 of WO2005033321), AAVhu.52 (SEQ ID NO: 191 of WO2005033321), AAVhu.53 (SEQ ID NO: 186 of WO2005033321), AAVhu.54 (SEQ ID NO: 188 of WO2005033321), AAVhu.55 (SEQ ID NO: 187 of WO2005033321), AAVhu.56 (SEQ ID NO: 192 of WO2005033321), AAVhu.57 (SEQ ID NO: 193 of WO2005033321), AAVhu.58 (SEQ ID NO: 194 of WO2005033321), AAVhu.6 (SEQ ID NO: 84 of WO2005033321), AAVhu.60 (SEQ ID NO: 184 of WO2005033321), AAVhu.61 (SEQ ID NO: 185 of WO2005033321), AAVhu.63 (SEQ ID NO: 195 of WO2005033321), AAVhu.64 (SEQ ID NO: 196 of WO2005033321), AAVhu.66 (SEQ ID NO: 197 of WO2005033321), AAVhu.67 (SEQ ID NO: 198 of WO2005033321), AAVhu.7 (SEQ ID NO: 150 of WO2005033321), AAVhu.8 (WO2005033321 SEQ ID NO: 12), AAVhu.9 (SEQ ID NO: 155 of WO2005033321), AAVLG-10/rh.40 (SEQ ID No: 14 of WO2005033321), AAVLG-4/rh.38 (SEQ ID NO: 86 of WO2005033321), AAVLG-4/rh.38 (SEQ ID No: 7 of WO2005033321), AAVN721-8/rh.43 (SEQ ID NO: 163 of WO2005033321), AAVN721-8/rh.43 (SEQ ID No: 43 of WO2005033321), AAVpi.1 (WO2005033321 SEQ ID NO: 28), AAVpi.2 (WO2005033321 SEQ ID NO: 30), AAVpi.3 (WO2005033321 SEQ ID NO: 29), AAVrh.38 (SEQ ID NO: 86 of WO2005033321), AAVrh.40 (SEQ ID NO: 92 of WO2005033321), AAVrh.43 (SEQ ID NO: 163 of WO2005033321), AAVrh.44 (WO2005033321 SEQ ID NO: 34), AAVrh.45 (WO2005033321 SEQ ID NO: 41), AAVrh.47 (WO2005033321 SEQ ID NO: 38), AAVrh.48 (SEQ ID NO: 115 of WO2005033321), AAVrh.49 (SEQ ID NO: 103 of WO2005033321), AAVrh.50 (SEQ ID NO: 108 of WO2005033321), AAVrh.51 (SEQ ID NO: 104 of WO2005033321), AAVrh.52 (SEQ ID NO: 96 of WO2005033321), AAVrh.53 (SEQ ID NO: 97 of WO2005033321), AAVrh.55 (WO2005033321 SEQ ID NO: 37), AAVrh.56 (SEQ ID NO: 152 of WO2005033321), AAVrh.57 (SEQ ID NO: 105 of WO2005033321), AAVrh.58 (SEQ ID NO: 106 of WO2005033321), AAVrh.59 (WO2005033321 SEQ ID NO: 42), AAVrh.60 (WO2005033321 SEQ ID NO: 31), AAVrh.61 (SEQ ID NO: 107 of WO2005033321), AAVrh.62 (SEQ ID NO: 114 of WO2005033321), AAVrh.64 (SEQ ID NO: 99 of WO2005033321), AAVrh.65 (WO2005033321 SEQ ID NO: 35), AAVrh.68 (WO2005033321 SEQ ID NO: 16), AAVrh.69 (WO2005033321 SEQ ID NO: 39), AAVrh.70 (WO2005033321 SEQ ID NO: 20), AAVrh.72 (WO2005033321 SEQ ID NO: 9), or variants thereof including, but not limited to, AAVcy.2, AAVcy.3, AAVcy.4, AAVcy.5, AAVcy.6, AAVrh.12, AAVrh.17, AAVrh.18, AAVrh.19, AAVrh.21, AAVrh.22, AAVrh.23, AAVrh.24, AAVrh.25, AAVrh.25/42 15, AAVrh.31, AAVrh.32, AAVrh.33, AAVrh.34, AAVrh.35, AAVrh.36, AAVrh.37, AAVrh14. Non limiting examples of variants include SEQ ID NO: 13, 15, 17, 19, 24, 36, 40, 45, 47, 48, 51-54, 60-62, 64-77, 79, 80, 82, 89, 90, 93-95, 98, 100, 101, 109-113, 118-120, 124, 126, 131, 139, 142, 151, 154, 158, 161, 162, 165-183, 202, 204-212, 215, 219, 224-236, of WO2005033321, the contents of which are herein incorporated by reference in their entirety.

In some embodiments, the AAV serotype, the parent AAV capsid polypeptide, or the AAV capsid variant may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence as described in International Publication No. WO2015168666, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAVrh8R (SEQ ID NO: 9 of WO2015168666), AAVrh8R A586R mutant (SEQ ID NO: 10 of WO2015168666), AAVrh8R R533A mutant (SEQ ID NO: 11 of WO2015168666), or variants thereof.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence as described in U.S. Pat. No. 9,233,131, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAVhE1.1 (SEQ ID NO:44 of U.S. Pat. No. 9,233,131), AAVhEr1.5 (SEQ ID NO:45 of U.S. Pat. No. 9,233,131), AAVhER1.14 (SEQ ID NO:46 of U.S. Pat. No. 9,233,131), AAVhEr1.8 (SEQ ID NO:47 of U.S. Pat. No. 9,233,131), AAVhEr1.16 (SEQ ID NO:48 of U.S. Pat. No. 9,233,131), AAVhEr1.18 (SEQ ID NO:49 of U.S. Pat. No. 9,233,131), AAVhEr1.35 (SEQ ID NO:50 of U.S. Pat. No. 9,233,131), AAVhEr1.7 (SEQ ID NO:51 of U.S. Pat. No. 9,233,131), AAVhEr1.36 (SEQ ID NO:52 of U.S. Pat. No. 9,233,131), AAVhEr2.29 (SEQ ID NO:53 of U.S. Pat. No. 9,233,131), AAVhEr2.4 (SEQ ID NO:54 of U.S. Pat. No. 9,233,131), AAVhEr2.16 (SEQ ID NO:55 of U.S. Pat. No. 9,233,131), AAVhEr2.30 (SEQ ID NO:56 of U.S. Pat. No. 9,233,131), AAVhEr2.31 (SEQ ID NO:58 of U.S. Pat. No. 9,233,131), AAVhEr2.36 (SEQ ID NO:57 of U.S. Pat. No. 9,233,131), AAVhER1.23 (SEQ ID NO:53 of U.S. Pat. No. 9,233,131), AAVhEr3.1 (SEQ ID NO:59 of U.S. Pat. No. 9,233,131), AAV2.5T (SEQ ID NO:42 of U.S. Pat. No. 9,233,131), or variants thereof.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence as described in United States Patent Publication No. US20150376607, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAV-PAEC (SEQ ID NO:1 of US20150376607), AAV-LK01 (SEQ ID NO:2 of US20150376607), AAV-LK02 (SEQ ID NO:3 of US20150376607), AAV-LK03 (SEQ ID NO:4 of US20150376607), AAV-LK04 (SEQ ID NO:5 of US20150376607), AAV-LK05 (SEQ ID NO:6 of US20150376607), AAV-LK06 (SEQ ID NO:7 of US20150376607), AAV-LK07 (SEQ ID NO:8 of US20150376607), AAV-LK08 (SEQ ID NO:9 of US20150376607), AAV-LK09 (SEQ ID NO:10 of US20150376607), AAV-LK10 (SEQ ID NO:11 of US20150376607), AAV-LK11 (SEQ ID NO:12 of US20150376607), AAV-LK12 (SEQ ID NO:13 of US20150376607), AAV-LK13 (SEQ ID NO:14 of US20150376607), AAV-LK14 (SEQ ID NO:15 of US20150376607), AAV-LK15 (SEQ ID NO:16 of US20150376607), AAV-LK16 (SEQ ID NO:17 of US20150376607), AAV-LK17 (SEQ ID NO:18 of US20150376607), AAV-LK18 (SEQ ID NO:19 of US20150376607), AAV-LK19 (SEQ ID NO:20 of US20150376607), AAV-PAEC2 (SEQ ID NO:21 of US20150376607), AAV-PAEC4 (SEQ ID NO:22 of US20150376607), AAV-PAEC6 (SEQ ID NO:23 of US20150376607), AAV-PAEC7 (SEQ ID NO:24 of US20150376607), AAV-PAEC8 (SEQ ID NO:25 of US20150376607), AAV-PAEC11 (SEQ ID NO:26 of US20150376607), AAV-PAEC12 (SEQ ID NO:27, of US20150376607), or variants thereof.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence as described in U.S. Pat. No. 9,163,261, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAV-2-pre-miRNA-101 (SEQ ID NO: 1 U.S. Pat. No. 9,163,261), or variants thereof.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence as described in United States Patent Publication No. US20150376240, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAV-8h (SEQ ID NO: 6 of US20150376240), AAV-8b (SEQ ID NO: 5 of US20150376240), AAV-h (SEQ ID NO: 2 of US20150376240), AAV-b (SEQ ID NO: 1 of US20150376240), or variants thereof.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence as described in United States Patent Publication No. US20160017295, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAV SM 10-2 (SEQ ID NO: 22 of US20160017295), AAV Shuffle 100-1 (SEQ ID NO: 23 of US20160017295), AAV Shuffle 100-3 (SEQ ID NO: 24 of US20160017295), AAV Shuffle 100-7 (SEQ ID NO: 25 of US20160017295), AAV Shuffle 10-2 (SEQ ID NO: 34 of US20160017295), AAV Shuffle 10-6 (SEQ ID NO: 35 of US20160017295), AAV Shuffle 10-8 (SEQ ID NO: 36 of US20160017295), AAV Shuffle 100-2 (SEQ ID NO: 37 of US20160017295), AAV SM 10-1 (SEQ ID NO: 38 of US20160017295), AAV SM 10-8 (SEQ ID NO: 39 of US20160017295), AAV SM 100-3 (SEQ ID NO: 40 of US20160017295), AAV SM 100-10 (SEQ ID NO: 41 of US20160017295), or variants thereof.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence as described in United States Patent Publication No. US20150238550, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, BNP61 AAV (SEQ ID NO: 1 of US20150238550), BNP62 AAV (SEQ ID NO: 3 of US20150238550), BNP63 AAV (SEQ ID NO: 4 of US20150238550), or variants thereof.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence as described in United States Patent Publication No. US20150315612, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAVrh.50 (SEQ ID NO: 108 of US20150315612), AAVrh.43 (SEQ ID NO: 163 of US20150315612), AAVrh.62 (SEQ ID NO: 114 of US20150315612), AAVrh.48 (SEQ ID NO: 115 of US20150315612), AAVhu.19 (SEQ ID NO: 133 of US20150315612), AAVhu.11 (SEQ ID NO: 153 of US20150315612), AAVhu.53 (SEQ ID NO: 186 of US20150315612), AAV4-8/rh.64 (SEQ ID No: 15 of US20150315612), AAVLG-9/hu.39 (SEQ ID No: 24 of US20150315612), AAV54.5/hu.23 (SEQ ID No: 60 of US20150315612), AAV54.2/hu.22 (SEQ ID No: 67 of US20150315612), AAV54.7/hu.24 (SEQ ID No: 66 of US20150315612), AAV54.1/hu.21 (SEQ ID No: 65 of US20150315612), AAV54.4R/hu.27 (SEQ ID No: 64 of US20150315612), AAV46.2/hu.28 (SEQ ID No: 68 of US20150315612), AAV46.6/hu.29 (SEQ ID No: 69 of US20150315612), AAV128.1/hu.43 (SEQ ID No: 80 of US20150315612), or variants thereof.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence as described in International Publication No. WO2015121501, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, true type AAV (ttAAV) (SEQ ID NO: 2 of WO2015121501), “UPenn AAV10” (SEQ ID NO: 8 of WO2015121501), “Japanese AAV10” (SEQ ID NO: 9 of WO2015121501), or variants thereof.

According to the present disclosure, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid, may be selected or derived from a variety of species. In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid, may be, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, an avian AAV (AAAV). In some embodiments, the AAV serotype, the parent AAV capsid polypeptide, or the AAV capsid variant may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence as described in U.S. Pat. No. 9,238,800, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAAV (SEQ ID NO: 1, 2, 4, 6, 8, 10, 12, and 14 of U.S. Pat. No. 9,238,800), or variants thereof.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence as described in U.S. Pat. No. 9,193,769, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, BAAV (SEQ ID NO: 1 and 6 of U.S. Pat. No. 9,193,769), or variants thereof. In some embodiments, the AAV serotype, the parent AAV capsid polypeptide, or the AAV capsid variant may be, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a BAAV serotype comprising a sequence as described in U.S. Pat. No. 7,427,396, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, BAAV (SEQ ID NO: 5 and 6 of U.S. Pat. No. 7,427,396), or variants thereof.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be a caprine AAV. In some embodiments, the AAV serotype, the parent AAV capsid polypeptide, or the AAV capsid variant may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, may be a caprine AAV serotype comprising a sequence as described in U.S. Pat. No. 7,427,396, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, caprine AAV (SEQ ID NO: 3 of U.S. Pat. No. 7,427,396), or variants thereof.

In other embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be engineered as a hybrid AAV from two or more serotypes, e.g., parental serotypes. In some embodiments, the AAV serotype, the parent AAV capsid polypeptide, or the AAV capsid variant may be, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, may be a AAV2G9, which comprises sequences from AAV2 and AAV9, e.g., wherein the AAV2G9 AAV serotype may be, or have, a sequence as described in United States Patent Publication No. US20160017005, the contents of which are herein incorporated by reference in its entirety.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138 a serotype generated by the AAV9 capsid library with mutations in amino acids 390-627 (VP1 numbering) as described by Pulicherla et al. (Molecular Therapy 19(6):1070-1078 (2011), the contents of which are herein incorporated by reference in their entirety. The serotype and corresponding nucleotide and amino acid substitutions may be, but is not limited to, AAV9.1 (G1594C; D532H), AAV6.2 (T1418A and T1436X; V473D and I479K), AAV9.3 (T1238A; F413Y), AAV9.4 (T1250C and A1617T; F417S), AAV9.5 (A1235G, A1314T, A1642G, C1760T; Q412R, T548A, A587V), AAV9.6 (T1231A; F411I), AAV9.9 (G1203A, G1785T; W595C), AAV9.10 (A1500G, T1676C; M559T), AAV9.11 (A1425T, A1702C, A1769T; T568P, Q590L), AAV9.13 (A1369C, A1720T; N457H, T574S), AAV9.14 (T1340A, T1362C, T1560C, G1713A; L447H), AAV9.16 (A1775T; Q592L), AAV9.24 (T1507C, T1521G; W503R), AAV9.26 (A1337G, A1769C; Y446C, Q590P), AAV9.33 (A1667C; D556A), AAV9.34 (A1534G, C1794T; N512D), AAV9.35 (A1289T, T1450A, C1494T, A1515T, C1794A, G1816A; Q430L, Y484N, N98K, V6061), AAV9.40 (A1694T, E565V), AAV9.41 (A1348T, T1362C; T450S), AAV9.44 (A1684C, A1701T, A1737G; N562H, K567N), AAV9.45 (A1492T, C1804T; N498Y, L602F), AAV9.46 (G1441C, T1525C, T1549G; G481R, W509R, L517V), 9.47 (G1241A, G1358A, A1669G, C1745T; S414N, G453D, K557E, T582I), AAV9.48 (C1445T, A1736T; P482L, Q579L), AAV9.50 (A1638T, C1683T, T1805A; Q546H, L602H), AAV9.53 (G1301A, A1405C, C1664T, G1811T; R134Q, S469R, A555V, G604V), AAV9.54 (C1531A, T1609A; L511I, L537M), AAV9.55 (T1605A; F535L), AAV9.58 (C1475T, C1579A; T492I, H527N), AAV.59 (T1336C; Y446H), AAV9.61 (A1493T; N498I), AAV9.64 (C1531A, A1617T; L511I), AAV9.65 (C1335T, T1530C, C1568A; A523D), AAV9.68 (C1510A; P504T), AAV9.80 (G1441A, G481R), AAV9.83 (C1402A, A1500T; P468T, E500D), AAV9.87 (T1464C, T1468C; S490P), AAV9.90 (A1196T; Y399F), AAV9.91 (T1316G, A1583T, C1782G, T1806C; L439R, K528I), AAV9.93 (A1273G, A1421G, A1638C, C1712T, G1732A, A1744T, A1832T; S425G, Q474R, Q546H, P571L, G578R, T582S, D611V), AAV9.94 (A1675T; M559L) and AAV9.95 (T1605A; F535L).

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence as described in International Publication No. WO2016049230, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to AAVF1/HSC1 (SEQ ID NO: 2 and 20 of WO2016049230), AAVF2/HSC2 (SEQ ID NO: 3 and 21 of WO2016049230), AAVF3/HSC3 (SEQ ID NO: 5 and 22 of WO2016049230), AAVF4/HSC4 (SEQ ID NO: 6 and 23 of WO2016049230), AAVF5/HSC5 (SEQ ID NO: 11 and 25 of WO2016049230), AAVF6/HSC6 (SEQ ID NO: 7 and 24 of WO2016049230), AAVF7/HSC7 (SEQ ID NO: 8 and 27 of WO2016049230), AAVF8/HSC8 (SEQ ID NO: 9 and 28 of WO2016049230), AAVF9/HSC9 (SEQ ID NO: 10 and 29 of WO2016049230), AAVF11/HSC11 (SEQ ID NO: 4 and 26 of WO2016049230), AAVF12/HSC12 (SEQ ID NO: 12 and 30 of WO2016049230), AAVF13/HSC13 (SEQ ID NO: 14 and 31 of WO2016049230), AAVF14/HSC14 (SEQ ID NO: 15 and 32 of WO2016049230), AAVF15/HSC15 (SEQ ID NO: 16 and 33 of WO2016049230), AAVF16/HSC16 (SEQ ID NO: 17 and 34 of WO2016049230), AAVF17/HSC17 (SEQ ID NO: 13 and 35 of WO2016049230), or variants or derivatives thereof.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence as described in U.S. Pat. No. 8,734,809, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAV CBr-E1 (SEQ ID NO: 13 and 87 of U.S. Pat. No. 8,734,809), AAV CBr-E2 (SEQ ID NO: 14 and 88 of U.S. Pat. No. 8,734,809), AAV CBr-E3 (SEQ ID NO: 15 and 89 of U.S. Pat. No. 8,734,809), AAV CBr-E4 (SEQ ID NO: 16 and 90 of U.S. Pat. No. 8,734,809), AAV CBr-E5 (SEQ ID NO: 17 and 91 of U.S. Pat. No. 8,734,809), AAV CBr-e5 (SEQ ID NO: 18 and 92 of U.S. Pat. No. 8,734,809), AAV CBr-E6 (SEQ ID NO: 19 and 93 of U.S. Pat. No. 8,734,809), AAV CBr-E7 (SEQ ID NO: 20 and 94 of U.S. Pat. No. 8,734,809), AAV CBr-E8 (SEQ ID NO: 21 and 95 of U.S. Pat. No. 8,734,809), AAV CLv-D1 (SEQ ID NO: 22 and 96 of U.S. Pat. No. 8,734,809), AAV CLv-D2 (SEQ ID NO: 23 and 97 of U.S. Pat. No. 8,734,809), AAV CLv-D3 (SEQ ID NO: 24 and 98 of U.S. Pat. No. 8,734,809), AAV CLv-D4 (SEQ ID NO: 25 and 99 of U.S. Pat. No. 8,734,809), AAV CLv-D5 (SEQ ID NO: 26 and 100 of U.S. Pat. No. 8,734,809), AAV CLv-D6 (SEQ ID NO: 27 and 101 of U.S. Pat. No. 8,734,809), AAV CLv-D7 (SEQ ID NO: 28 and 102 of U.S. Pat. No. 8,734,809), AAV CLv-D8 (SEQ ID NO: 29 and 103 of U.S. Pat. No. 8,734,809), AAV CLv-E1 (SEQ ID NO: 13 and 87 of U.S. Pat. No. 8,734,809), AAV CLv-R1 (SEQ ID NO: 30 and 104 of U.S. Pat. No. 8,734,809), AAV CLv-R2 (SEQ ID NO: 31 and 105 of U.S. Pat. No. 8,734,809), AAV CLv-R3 (SEQ ID NO: 32 and 106 of U.S. Pat. No. 8,734,809), AAV CLv-R4 (SEQ ID NO: 33 and 107 of U.S. Pat. No. 8,734,809), AAV CLv-R5 (SEQ ID NO: 34 and 108 of U.S. Pat. No. 8,734,809), AAV CLv-R6 (SEQ ID NO: 35 and 109 of U.S. Pat. No. 8,734,809), AAV CLv-R7 (SEQ ID NO: 36 and 110 of U.S. Pat. No. 8,734,809), AAV CLv-R8 (SEQ ID NO: X and X of U.S. Pat. No. 8,734,809), AAV CLv-R9 (SEQ ID NO: X and X of U.S. Pat. No. 8,734,809), AAV CLg-F1 (SEQ ID NO: 39 and 113 of U.S. Pat. No. 8,734,809), AAV CLg-F2 (SEQ ID NO: 40 and 114 of U.S. Pat. No. 8,734,809), AAV CLg-F3 (SEQ ID NO: 41 and 115 of U.S. Pat. No. 8,734,809), AAV CLg-F4 (SEQ ID NO: 42 and 116 of U.S. Pat. No. 8,734,809), AAV CLg-F5 (SEQ ID NO: 43 and 117 of U.S. Pat. No. 8,734,809), AAV CLg-F6 (SEQ ID NO: 43 and 117 of U.S. Pat. No. 8,734,809), AAV CLg-F7 (SEQ ID NO: 44 and 118 of U.S. Pat. No. 8,734,809), AAV CLg-F8 (SEQ ID NO: 43 and 117 of U.S. Pat. No. 8,734,809), AAV CSp-1 (SEQ ID NO: 45 and 119 of U.S. Pat. No. 8,734,809), AAV CSp-10 (SEQ ID NO: 46 and 120 of U.S. Pat. No. 8,734,809), AAV CSp-11 (SEQ ID NO: 47 and 121 of U.S. Pat. No. 8,734,809), AAV CSp-2 (SEQ ID NO: 48 and 122 of U.S. Pat. No. 8,734,809), AAV CSp-3 (SEQ ID NO: 49 and 123 of U.S. Pat. No. 8,734,809), AAV CSp-4 (SEQ ID NO: 50 and 124 of U.S. Pat. No. 8,734,809), AAV CSp-6 (SEQ ID NO: 51 and 125 of U.S. Pat. No. 8,734,809), AAV CSp-7 (SEQ ID NO: 52 and 126 of U.S. Pat. No. 8,734,809), AAV CSp-8 (SEQ ID NO: 53 and 127 of U.S. Pat. No. 8,734,809), AAV CSp-9 (SEQ ID NO: 54 and 128 of U.S. Pat. No. 8,734,809), AAV CHt-2 (SEQ ID NO: 55 and 129 of U.S. Pat. No. 8,734,809), AAV CHt-3 (SEQ ID NO: 56 and 130 of U.S. Pat. No. 8,734,809), AAV CKd-1 (SEQ ID NO: 57 and 131 of U.S. Pat. No. 8,734,809), AAV CKd-10 (SEQ ID NO: 58 and 132 of U.S. Pat. No. 8,734,809), AAV CKd-2 (SEQ ID NO: 59 and 133 of U.S. Pat. No. 8,734,809), AAV CKd-3 (SEQ ID NO: 60 and 134 of U.S. Pat. No. 8,734,809), AAV CKd-4 (SEQ ID NO: 61 and 135 of U.S. Pat. No. 8,734,809), AAV CKd-6 (SEQ ID NO: 62 and 136 of U.S. Pat. No. 8,734,809), AAV CKd-7 (SEQ ID NO: 63 and 137 of U.S. Pat. No. 8,734,809), AAV CKd-8 (SEQ ID NO: 64 and 138 of U.S. Pat. No. 8,734,809), AAV CLv-1 (SEQ ID NO: 35 and 139 of U.S. Pat. No. 8,734,809), AAV CLv-12 (SEQ ID NO: 66 and 140 of U.S. Pat. No. 8,734,809), AAV CLv-13 (SEQ ID NO: 67 and 141 of U.S. Pat. No. 8,734,809), AAV CLv-2 (SEQ ID NO: 68 and 142 of U.S. Pat. No. 8,734,809), AAV CLv-3 (SEQ ID NO: 69 and 143 of U.S. Pat. No. 8,734,809), AAV CLv-4 (SEQ ID NO: 70 and 144 of U.S. Pat. No. 8,734,809), AAV CLv-6 (SEQ ID NO: 71 and 145 of U.S. Pat. No. 8,734,809), AAV CLv-8 (SEQ ID NO: 72 and 146 of U.S. Pat. No. 8,734,809), AAV CKd-B1 (SEQ ID NO: 73 and 147 of U.S. Pat. No. 8,734,809), AAV CKd-B2 (SEQ ID NO: 74 and 148 of U.S. Pat. No. 8,734,809), AAV CKd-B3 (SEQ ID NO: 75 and 149 of U.S. Pat. No. 8,734,809), AAV CKd-B4 (SEQ ID NO: 76 and 150 of U.S. Pat. No. 8,734,809), AAV CKd-B5 (SEQ ID NO: 77 and 151 of U.S. Pat. No. 8,734,809), AAV CKd-B6 (SEQ ID NO: 78 and 152 of U.S. Pat. No. 8,734,809), AAV CKd-B7 (SEQ ID NO: 79 and 153 of U.S. Pat. No. 8,734,809), AAV CKd-B8 (SEQ ID NO: 80 and 154 of U.S. Pat. No. 8,734,809), AAV CKd-H1 (SEQ ID NO: 81 and 155 of U.S. Pat. No. 8,734,809), AAV CKd-H2 (SEQ ID NO: 82 and 156 of U.S. Pat. No. 8,734,809), AAV CKd-H3 (SEQ ID NO: 83 and 157 of U.S. Pat. No. 8,734,809), AAV CKd-H4 (SEQ ID NO: 84 and 158 of U.S. Pat. No. 8,734,809), AAV CKd-H5 (SEQ ID NO: 85 and 159 of U.S. Pat. No. 8,734,809), AAV CKd-H6 (SEQ ID NO: 77 and 151 of U.S. Pat. No. 8,734,809), AAV CHt-1 (SEQ ID NO: 86 and 160 of U.S. Pat. No. 8,734,809), AAV CLv1-1 (SEQ ID NO: 171 of U.S. Pat. No. 8,734,809), AAV CLv1-2 (SEQ ID NO: 172 of U.S. Pat. No. 8,734,809), AAV CLv1-3 (SEQ ID NO: 173 of U.S. Pat. No. 8,734,809), AAV CLv1-4 (SEQ ID NO: 174 of U.S. Pat. No. 8,734,809), AAV Clv1-7 (SEQ ID NO: 175 of U.S. Pat. No. 8,734,809), AAV Clv1-8 (SEQ ID NO: 176 of U.S. Pat. No. 8,734,809), AAV Clv1-9 (SEQ ID NO: 177 of U.S. Pat. No. 8,734,809), AAV Clv1-10 (SEQ ID NO: 178 of U.S. Pat. No. 8,734,809), AAV.VR-355 (SEQ ID NO: 181 of U.S. Pat. No. 8,734,809), AAV.hu.48R3 (SEQ ID NO: 183 of U.S. Pat. No. 8,734,809), or variants or derivatives thereof.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence as described in International Publication No. WO2016065001, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to AAV CHt-P2 (SEQ ID NO: 1 and 51 of WO2016065001), AAV CHt-P5 (SEQ ID NO: 2 and 52 of WO2016065001), AAV CHt-P9 (SEQ ID NO: 3 and 53 of WO2016065001), AAV CBr-7.1 (SEQ ID NO: 4 and 54 of WO2016065001), AAV CBr-7.2 (SEQ ID NO: 5 and 55 of WO2016065001), AAV CBr-7.3 (SEQ ID NO: 6 and 56 of WO2016065001), AAV CBr-7.4 (SEQ ID NO: 7 and 57 of WO2016065001), AAV CBr-7.5 (SEQ ID NO: 8 and 58 of WO2016065001), AAV CBr-7.7 (SEQ ID NO: 9 and 59 of WO2016065001), AAV CBr-7.8 (SEQ ID NO: 10 and 60 of WO2016065001), AAV CBr-7.10 (SEQ ID NO: 11 and 61 of WO2016065001), AAV CKd-N3 (SEQ ID NO: 12 and 62 of WO2016065001), AAV CKd-N4 (SEQ ID NO: 13 and 63 of WO2016065001), AAV CKd-N9 (SEQ ID NO: 14 and 64 of WO2016065001), AAV CLv-L4 (SEQ ID NO: 15 and 65 of WO2016065001), AAV CLv-L5 (SEQ ID NO: 16 and 66 of WO2016065001), AAV CLv-L6 (SEQ ID NO: 17 and 67 of WO2016065001), AAV CLv-K1 (SEQ ID NO: 18 and 68 of WO2016065001), AAV CLv-K3 (SEQ ID NO: 19 and 69 of WO2016065001), AAV CLv-K6 (SEQ ID NO: 20 and 70 of WO2016065001), AAV CLv-M1 (SEQ ID NO: 21 and 71 of WO2016065001), AAV CLv-M11 (SEQ ID NO: 22 and 72 of WO2016065001), AAV CLv-M2 (SEQ ID NO: 23 and 73 of WO2016065001), AAV CLv-M5 (SEQ ID NO: 24 and 74 of WO2016065001), AAV CLv-M6 (SEQ ID NO: 25 and 75 of WO2016065001), AAV CLv-M7 (SEQ ID NO: 26 and 76 of WO2016065001), AAV CLv-M8 (SEQ ID NO: 27 and 77 of WO2016065001), AAV CLv-M9 (SEQ ID NO: 28 and 78 of WO2016065001), AAV CHt-P1 (SEQ ID NO: 29 and 79 of WO2016065001), AAV CHt-P6 (SEQ ID NO: 30 and 80 of WO2016065001), AAV CHt-P8 (SEQ ID NO: 31 and 81 of WO2016065001), AAV CHt-6.1 (SEQ ID NO: 32 and 82 of WO2016065001), AAV CHt-6.10 (SEQ ID NO: 33 and 83 of WO2016065001), AAV CHt-6.5 (SEQ ID NO: 34 and 84 of WO2016065001), AAV CHt-6.6 (SEQ ID NO: 35 and 85 of WO2016065001), AAV CHt-6.7 (SEQ ID NO: 36 and 86 of WO2016065001), AAV CHt-6.8 (SEQ ID NO: 37 and 87 of WO2016065001), AAV CSp-8.10 (SEQ ID NO: 38 and 88 of WO2016065001), AAV CSp-8.2 (SEQ ID NO: 39 and 89 of WO2016065001), AAV CSp-8.4 (SEQ ID NO: 40 and 90 of WO2016065001), AAV CSp-8.5 (SEQ ID NO: 41 and 91 of WO2016065001), AAV CSp-8.6 (SEQ ID NO: 42 and 92 of WO2016065001), AAV CSp-8.7 (SEQ ID NO: 43 and 93 of WO2016065001), AAV CSp-8.8 (SEQ ID NO: 44 and 94 of WO2016065001), AAV CSp-8.9 (SEQ ID NO: 45 and 95 of WO2016065001), AAV CBr-B7.3 (SEQ ID NO: 46 and 96 of WO2016065001), AAV CBr-B7.4 (SEQ ID NO: 47 and 97 of WO2016065001), AAV3B (SEQ ID NO: 48 and 98 of WO2016065001), AAV4 (SEQ ID NO: 49 and 99 of WO2016065001), AAV5 (SEQ ID NO: 50 and 100 of WO2016065001), or variants or derivatives thereof.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a serotype selected from any of those found in Table 6.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence, fragment or variant thereof, of any of the sequences in Table 6.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be encoded by, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence, fragment or variant as described in Table 6.

TABLE 6 AAV Sequences SEQ ID Serotype NO: Reference Information VOY101 1 — VOY101 2 — VOY201 3 — VOY201 1724 — PHP.N/PHP.B-DGT 4 WO2017100671 SEQ ID NO: 46 AAVPHP.B or G2B-26 5 WO2015038958 SEQ ID NO: 8 and 13 AAVPHP.B 6 WO2015038958 SEQ ID NO: 9 AAVG2B-13 7 WO2015038958 SEQ ID NO: 12 AAVTH1.1-32 8 WO2015038958 SEQ ID NO: 14 AAVTH1.1-35 9 WO2015038958 SEQ ID NO: 15 PHP.S/G2A12 10 WO2017100671 SEQ ID NO: 47 AAV9/hu.14 K449R 11 WO2017100671 SEQ ID NO: 45 AAV1 12 U520150159173 SEQ ID NO: 11, US20150315612 SEQ ID NO: 202 AAV1 13 U520160017295 SEQ ID NO: 1, U520030138772 SEQ ID NO: 64, US20150159173 SEQ ID NO: 27, US20150315612 SEQ ID NO: 219, U57198951 SEQ ID NO: 5 AAV1 14 U520030138772 SEQ ID NO: 6 AAV1.3 15 U520030138772 SEQ ID NO: 14 AAV10 16 U520030138772 SEQ ID NO: 117 AAV10 17 WO2015121501 SEQ ID NO: 9 AAV10 18 WO2015121501 SEQ ID NO: 8 AAV11 19 U520030138772 SEQ ID NO: 118 AAV12 20 U520030138772 SEQ ID NO: 119 AAV2 21 U520150159173 SEQ ID NO: 7, US20150315612 SEQ ID NO: 211 AAV2 22 U520030138772 SEQ ID NO: 70, U520150159173 SEQ ID NO: 23, U520150315612 SEQ ID NO: 221, U520160017295 SEQ ID NO: 2, US6156303 SEQ ID NO: 4, U57198951 SEQ ID NO: 4, WO2015121501 SEQ ID NO: 1 AAV2 23 US6156303 SEQ ID NO: 8 AAV2 24 US20030138772 SEQ ID NO: 7 AAV2 25 US6156303 SEQ ID NO: 3 AAV2.5T 26 US9233131 SEQ ID NO: 42 AAV223.10 27 US20030138772 SEQ ID NO: 75 AAV223.2 28 US20030138772 SEQ ID NO: 49 AAV223.2 29 US20030138772 SEQ ID NO: 76 AAV223.4 30 US20030138772 SEQ ID NO: 50 AAV223.4 31 US20030138772 SEQ ID NO: 73 AAV223.5 32 U520030138772 SEQ ID NO: 51 AAV223.5 33 US20030138772 SEQ ID NO: 74 AAV223.6 34 US20030138772 SEQ ID NO: 52 AAV223.6 35 US20030138772 SEQ ID NO: 78 AAV223.7 36 US20030138772 SEQ ID NO: 53 AAV223.7 37 US20030138772 SEQ ID NO: 77 AAV29.3 38 US20030138772 SEQ ID NO: 82 AAV29.4 39 US20030138772 SEQ ID NO: 12 AAV29.5 40 US20030138772 SEQ ID NO: 83 AAV29.5 (AAVbb.2) 41 US20030138772 SEQ ID NO: 13 AAV3 42 U520150159173 SEQ ID NO: 12 AAV3 43 U520030138772 SEQ ID NO: 71, U520150159173 SEQ ID NO: 28, U520160017295 SEQ ID NO: 3, U57198951 SEQ ID NO: 6 AAV3 44 U520030138772 SEQ ID NO: 8 AAV3.3b 45 U520030138772 SEQ ID NO: 72 AAV3-3 46 U520150315612 SEQ ID NO: 200 AAV3-3 47 U520150315612 SEQ ID NO: 217 AAV3a 48 US6156303 SEQ ID NO: 5 AAV3a 49 U56156303 SEQ ID NO: 9 AAV3b 50 US6156303 SEQ ID NO: 6 AAV3b 51 US6156303 SEQ ID NO: 10 AAV3b 52 US6156303 SEQ ID NO: 1 AAV4 53 U520140348794 SEQ ID NO: 17 AAV4 54 U520140348794 SEQ ID NO: 5 AAV4 55 U520140348794 SEQ ID NO: 3 AAV4 56 U520140348794 SEQ ID NO: 14 AAV4 57 U520140348794 SEQ ID NO: 15 AAV4 58 U520140348794 SEQ ID NO: 19 AAV4 59 U520140348794 SEQ ID NO: 12 AAV4 60 US20140348794 SEQ ID NO: 13 AAV4 61 U520140348794 SEQ ID NO: 7 AAV4 62 U520140348794 SEQ ID NO: 8 AAV4 63 U520140348794 SEQ ID NO: 9 AAV4 64 U520140348794 SEQ ID NO: 2 AAV4 65 U520140348794 SEQ ID NO: 10 AAV4 66 U520140348794 SEQ ID NO: 11 AAV4 67 US20140348794 SEQ ID NO: 18 AAV4 68 U520030138772 SEQ ID NO: 63, U520160017295 SEQ ID NO: 4, U520140348794 SEQ ID NO: 4 AAV4 69 U520140348794 SEQ ID NO: 16 AAV4 70 U520140348794 SEQ ID NO: 20 AAV4 71 U520140348794 SEQ ID NO: 6 AAV4 72 U520140348794 SEQ ID NO: 1 AAV42.2 73 U520030138772 SEQ ID NO: 9 AAV42.2 74 US20030138772 SEQ ID NO: 102 AAV42.3b 75 US20030138772 SEQ ID NO: 36 AAV42.3B 76 U520030138772 SEQ ID NO: 107 AAV42.4 77 US20030138772 SEQ ID NO: 33 AAV42.4 78 US20030138772 SEQ ID NO: 88 AAV42.8 79 US20030138772 SEQ ID NO: 27 AAV42.8 80 US20030138772 SEQ ID NO: 85 AAV43.1 81 US20030138772 SEQ ID NO: 39 AAV43.1 82 US20030138772 SEQ ID NO: 92 AAV43.12 83 US20030138772 SEQ ID NO: 41 AAV43.12 84 US20030138772 SEQ ID NO: 93 AAV43.20 85 US20030138772 SEQ ID NO: 42 AAV43.20 86 US20030138772 SEQ ID NO: 99 AAV43.21 87 U520030138772 SEQ ID NO: 43 AAV43.21 88 U520030138772 SEQ ID NO: 96 AAV43.23 89 US20030138772 SEQ ID NO: 44 AAV43.23 90 US20030138772 SEQ ID NO: 98 AAV43.25 91 U520030138772 SEQ ID NO: 45 AAV43.25 92 US20030138772 SEQ ID NO: 97 AAV43.5 93 US20030138772 SEQ ID NO: 40 AAV43.5 94 US20030138772 SEQ ID NO: 94 AAV4-4 95 U520150315612 SEQ ID NO: 201 AAV4-4 96 US20150315612 SEQ ID NO: 218 AAV44.1 97 U520030138772 SEQ ID NO: 46 AAV44.1 98 U520030138772 SEQ ID NO: 79 AAV44.5 99 US20030138772 SEQ ID NO: 47 AAV44.5 100 US20030138772 SEQ ID NO: 80 AAV4407 101 U520150315612 SEQ ID NO: 90 AAV5 102 U57427396 SEQ ID NO: 1 AAV5 103 U520030138772 SEQ ID NO: 114 AAV5 104 U520160017295 SEQ ID NO: 5, U57427396 SEQ ID NO: 2, U520150315612 SEQ ID NO: 216 AAV5 105 U520150315612 SEQ ID NO: 199 AAV6 106 U520150159173 SEQ ID NO: 13 AAV6 107 U520030138772 SEQ ID NO: 65, U520150159173 SEQ ID NO: 29, U520160017295 SEQ ID NO: 6, U56156303 SEQ ID NO: 7 AAV6 108 U56156303 SEQ ID NO: 11 AAV6 109 U56156303 SEQ ID NO: 2 AAV6 110 U520150315612 SEQ ID NO: 203 AAV6 111 U520150315612 SEQ ID NO: 220 AAV6.1 112 U520150159173 AAV6.12 113 U520150159173 AAV6.2 114 U520150159173 AAV7 115 U520150159173 SEQ ID NO: 14 AAV7 116 U520150315612 SEQ ID NO: 183 AAV7 117 U520030138772 SEQ ID NO: 2, U520150159173 SEQ ID NO: 30, U520150315612 SEQ ID NO: 181, U520160017295 SEQ ID NO: 7 AAV7 118 U520030138772 SEQ ID NO: 3 AAV7 119 U520030138772 SEQ ID NO: 1, U520150315612 SEQ ID NO: 180 AAV7 120 U520150315612 SEQ ID NO: 213 AAV7 121 U520150315612 SEQ ID NO: 222 AAV8 122 U520150159173 SEQ ID NO: 15 AAV8 123 U520150376240 SEQ ID NO: 7 AAV8 124 U520030138772 SEQ ID NO: 4, US20150315612 SEQ ID NO: 182 AAV8 125 U520030138772 SEQ ID NO: 95, U520140359799 SEQ ID NO: 1, US20150159173 SEQ ID NO: 31, U520160017295 SEQ ID NO: 8, U57198951 SEQ ID NO: 7, US20150315612 SEQ ID NO: 223 AAV8 126 U520150376240 SEQ ID NO: 8 AAV8 127 U520150315612 SEQ ID NO: 214 AAV-8b 128 U520150376240 SEQ ID NO: 5 AAV-8b 129 U520150376240 SEQ ID NO: 3 AAV-8h 130 US20150376240 SEQ ID NO: 6 AAV-8h 131 US20150376240 SEQ ID NO: 4 AAV9 132 US20030138772 SEQ ID NO: 5 AAV9 133 US7198951 SEQ ID NO: 1 AAV9 134 US20160017295 SEQ ID NO: 9 AAV9 135 US20030138772 SEQ ID NO: 100, US7198951 SEQ ID NO: 2 AAV9 136 US7198951 SEQ ID NO: 3 AAV9 (AAVhu.14) 137 US7906111 SEQ ID NO: 3; WO2015038958 SEQ ID NO: 11 AAV9 (AAVhu.14) 138 US7906111 SEQ ID NO: 123; WO2015038958 SEQ ID NO: 2 AAVA3.1 139 US20030138772 SEQ ID NO: 120 AAVA3.3 140 U520030138772 SEQ ID NO: 57 AAVA3.3 141 U520030138772 SEQ ID NO: 66 AAVA3.4 142 US20030138772 SEQ ID NO: 54 AAVA3.4 143 US20030138772 SEQ ID NO: 68 AAVA3.5 144 US20030138772 SEQ ID NO: 55 AAVA3.5 145 US20030138772 SEQ ID NO: 69 AAVA3.7 146 US20030138772 SEQ ID NO: 56 AAVA3.7 147 US20030138772 SEQ ID NO: 67 AAV29.3 (AAVbb.1) 148 U520030138772 SEQ ID NO: 11 AAVC2 149 U520030138772 SEQ ID NO: 61 AAVCh.5 150 U520150159173 SEQ ID NO: 46, US20150315612 SEQ ID NO: 234 AAVcy.2 (AAV13.3) 151 U520030138772 SEQ ID NO: 15 AAV24.1 152 U520030138772 SEQ ID NO: 101 AAVcy.3 (AAV24.1) 153 U520030138772 SEQ ID NO: 16 AAV27.3 154 U520030138772 SEQ ID NO: 104 AAVcy.4 (AAV27.3) 155 US20030138772 SEQ ID NO: 17 AAVcy.5 156 U520150315612 SEQ ID NO: 227 AAV7.2 157 US20030138772 SEQ ID NO: 103 AAVcy.5 (AAV7.2) 158 US20030138772 SEQ ID NO: 18 AAV16.3 159 U520030138772 SEQ ID NO: 105 AAVcy.6 (AAV16.3) 160 U520030138772 SEQ ID NO: 10 AAVcy.5 161 U520150159173 SEQ ID NO: 8 AAVcy.5 162 U520150159173 SEQ ID NO: 24 AAVCy.5R1 163 U520150159173 AAVCy.5R2 164 U520150159173 AAVCy.5R3 165 U520150159173 AAVCy.5R4 166 U520150159173 AAVDJ 167 U520140359799 SEQ ID NO: 3, U57588772 SEQ ID NO: 2 AAVDJ 168 U520140359799 SEQ ID NO: 2, U57588772 SEQ ID NO: 1 AAVDJ-8 169 U57588772; Grimm et al 2008 AAVDJ-8 170 U57588772; Grimm et al 2008 AAVF5 171 U520030138772 SEQ ID NO: 110 AAVH2 172 U520030138772 SEQ ID NO: 26 AAVH6 173 U520030138772 SEQ ID NO: 25 AAVhE1.1 174 U59233131 SEQ ID NO: 44 AAVhEr1.14 175 U59233131 SEQ ID NO: 46 AAVhEr1.16 176 U59233131 SEQ ID NO: 48 AAVhEr1.18 177 U59233131 SEQ ID NO: 49 AAVhEr1.23 (AAVhEr2.29) 178 U59233131 SEQ ID NO: 53 AAVhEr1.35 179 U59233131 SEQ ID NO: 50 AAVhEr1.36 180 U59233131 SEQ ID NO: 52 AAVhEr1.5 181 U59233131 SEQ ID NO: 45 AAVhEr1.7 182 U59233131 SEQ ID NO: 51 AAVhEr1.8 183 U59233131 SEQ ID NO: 47 AAVhEr2.16 184 U59233131 SEQ ID NO: 55 AAVhEr2.30 185 U59233131 SEQ ID NO: 56 AAVhEr2.31 186 US9233131 SEQ ID NO: 58 AAVhEr2.36 187 US9233131 SEQ ID NO: 57 AAVhEr2.4 188 US9233131 SEQ ID NO: 54 AAVhEr3.1 189 US9233131 SEQ ID NO: 59 AAVhu.1 190 US20150315612 SEQ ID NO: 46 AAVhu.1 191 US20150315612 SEQ ID NO: 144 AAVhu.10 (AAV16.8) 192 US20150315612 SEQ ID NO: 56 AAVhu.10 (AAV16.8) 193 U520150315612 SEQ ID NO: 156 AAVhu.11 (AAV16.12) 194 U520150315612 SEQ ID NO: 57 AAVhu.11 (AAV16.12) 195 U520150315612 SEQ ID NO: 153 AAVhu.12 196 U520150315612 SEQ ID NO: 59 AAVhu.12 197 U520150315612 SEQ ID NO: 154 AAVhu.13 198 U520150159173 SEQ ID NO: 16, U520150315612 SEQ ID NO: 71 AAVhu.13 199 U520150159173 SEQ ID NO: 32, U520150315612 SEQ ID NO: 129 AAVhu.136.1 200 U520150315612 SEQ ID NO: 165 AAVhu.140.1 201 U520150315612 SEQ ID NO: 166 AAVhu.140.2 202 U520150315612 SEQ ID NO: 167 AAVhu.145.6 203 U520150315612 SEQ ID No: 178 AAVhu.15 204 U520150315612 SEQ ID NO: 147 AAVhu.15 (AAV33.4) 205 US20150315612 SEQ ID NO: 50 AAVhu.156.1 206 U520150315612 SEQ ID No: 179 AAVhu.16 207 U520150315612 SEQ ID NO: 148 AAVhu.16 (AAV33.8) 208 US20150315612 SEQ ID NO: 51 AAVhu.17 209 U520150315612 SEQ ID NO: 83 AAVhu.17 (AAV33.12) 210 U520150315612 SEQ ID NO: 4 AAVhu.172.1 211 U520150315612 SEQ ID NO: 171 AAVhu.172.2 212 U520150315612 SEQ ID NO: 172 AAVhu.173.4 213 U520150315612 SEQ ID NO: 173 AAVhu.173.8 214 U520150315612 SEQ ID NO: 175 AAVhu.18 215 U520150315612 SEQ ID NO: 52 AAVhu.18 216 U520150315612 SEQ ID NO: 149 AAVhu.19 217 U520150315612 SEQ ID NO: 62 AAVhu.19 218 U520150315612 SEQ ID NO: 133 AAVhu.2 219 U520150315612 SEQ ID NO: 48 AAVhu.2 220 U520150315612 SEQ ID NO: 143 AAVhu.20 221 U520150315612 SEQ ID NO: 63 AAVhu.20 222 U520150315612 SEQ ID NO: 134 AAVhu.21 223 U520150315612 SEQ ID NO: 65 AAVhu.21 224 U520150315612 SEQ ID NO: 135 AAVhu.22 225 U520150315612 SEQ ID NO: 67 AAVhu.22 226 U520150315612 SEQ ID NO: 138 AAVhu.23 227 U520150315612 SEQ ID NO: 60 AAVhu.23.2 228 U520150315612 SEQ ID NO: 137 AAVhu.24 229 U520150315612 SEQ ID NO: 66 AAVhu.24 230 U520150315612 SEQ ID NO: 136 AAVhu.25 231 US20150315612 SEQ ID NO: 49 AAVhu.25 232 U520150315612 SEQ ID NO: 146 AAVhu.26 233 U520150159173 SEQ ID NO: 17, U520150315612 SEQ ID NO: 61 AAVhu.26 234 U520150159173 SEQ ID NO: 33, U520150315612 SEQ ID NO: 139 AAVhu.27 235 U520150315612 SEQ ID NO: 64 AAVhu.27 236 U520150315612 SEQ ID NO: 140 AAVhu.28 237 U520150315612 SEQ ID NO: 68 AAVhu.28 238 U520150315612 SEQ ID NO: 130 AAVhu.29 239 U520150315612 SEQ ID NO: 69 AAVhu.29 240 US20150159173 SEQ ID NO: 42, US20150315612 SEQ ID NO: 132 AAVhu.29 241 US20150315612 SEQ ID NO: 225 AAVhu.29R 242 US20150159173 AAVhu.3 243 US20150315612 SEQ ID NO: 44 AAVhu.3 244 US20150315612 SEQ ID NO: 145 AAVhu.30 245 U520150315612 SEQ ID NO: 70 AAVhu.30 246 U520150315612 SEQ ID NO: 131 AAVhu.31 247 U520150315612 SEQ ID NO: 1 AAVhu.31 248 U520150315612 SEQ ID NO: 121 AAVhu.32 249 U520150315612 SEQ ID NO: 2 AAVhu.32 250 U520150315612 SEQ ID NO: 122 AAVhu.33 251 U520150315612 SEQ ID NO: 75 AAVhu.33 252 U520150315612 SEQ ID NO: 124 AAVhu.34 253 U520150315612 SEQ ID NO: 72 AAVhu.34 254 U520150315612 SEQ ID NO: 125 AAVhu.35 255 U520150315612 SEQ ID NO: 73 AAVhu.35 256 U520150315612 SEQ ID NO: 164 AAVhu.36 257 U520150315612 SEQ ID NO: 74 AAVhu.36 258 U520150315612 SEQ ID NO: 126 AAVhu.37 259 U520150159173 SEQ ID NO: 34, U520150315612 SEQ ID NO: 88 AAVhu.37 (AAV106.1) 260 U520150315612 SEQ ID NO: 10,U520150159173 SEQ ID NO: 18 AAVhu.38 261 U520150315612 SEQ ID NO: 161 AAVhu.39 262 U520150315612 SEQ ID NO: 102 AAVhu.39 (AAVLG-9) 263 US20150315612 SEQ ID NO: 24 AAVhu.4 264 U520150315612 SEQ ID NO: 47 AAVhu.4 265 U520150315612 SEQ ID NO: 141 AAVhu.40 266 U520150315612 SEQ ID NO: 87 AAVhu.40 (AAV114.3) 267 US20150315612 SEQ ID No: 11 AAVhu.41 268 U520150315612 SEQ ID NO: 91 AAVhu.41 (AAV127.2) 269 US20150315612 SEQ ID NO: 6 AAVhu.42 270 U520150315612 SEQ ID NO: 85 AAVhu.42 (AAV127.5) 271 US20150315612 SEQ ID NO: 8 AAVhu.43 272 U520150315612 SEQ ID NO: 160 AAVhu.43 273 US20150315612 SEQ ID NO: 236 AAVhu.43 (AAV128.1) 274 US20150315612 SEQ ID NO: 80 AAVhu.44 275 U520150159173 SEQ ID NO: 45, U520150315612 SEQ ID NO: 158 AAVhu.44 (AAV128.3) 276 US20150315612 SEQ ID NO: 81 AAVhu.44R1 277 U520150159173 AAVhu.44R2 278 U520150159173 AAVhu.44R3 279 U520150159173 AAVhu.45 280 U520150315612 SEQ ID NO: 76 AAVhu.45 281 U520150315612 SEQ ID NO: 127 AAVhu.46 282 U520150315612 SEQ ID NO: 82 AAVhu.46 283 U520150315612 SEQ ID NO: 159 AAVhu.46 284 US20150315612 SEQ ID NO: 224 AAVhu.47 285 U520150315612 SEQ ID NO: 77 AAVhu.47 286 U520150315612 SEQ ID NO: 128 AAVhu.48 287 U520150159173 SEQ ID NO: 38 AAVhu.48 288 U520150315612 SEQ ID NO: 157 AAVhu.48 (AAV130.4) 289 US20150315612 SEQ ID NO: 78 AAVhu.48R1 290 U520150159173 AAVhu.48R2 291 U520150159173 AAVhu.48R3 292 U520150159173 AAVhu.49 293 US20150315612 SEQ ID NO: 209 AAVhu.49 294 U520150315612 SEQ ID NO: 189 AAVhu.5 295 US20150315612 SEQ ID NO: 45 AAVhu.5 296 US20150315612 SEQ ID NO: 142 AAVhu.51 297 U520150315612 SEQ ID NO: 208 AAVhu.51 298 U520150315612 SEQ ID NO: 190 AAVhu.52 299 U520150315612 SEQ ID NO: 210 AAVhu.52 300 U520150315612 SEQ ID NO: 191 AAVhu.53 301 U520150159173 SEQ ID NO: 19 AAVhu.53 302 U520150159173 SEQ ID NO: 35 AAVhu.53 (AAV145.1) 303 US20150315612 SEQ ID NO: 176 AAVhu.54 304 U520150315612 SEQ ID NO: 188 AAVhu.54 (AAV145.5) 305 US20150315612 SEQ ID No: 177 AAVhu.55 306 U520150315612 SEQ ID NO: 187 AAVhu.56 307 US20150315612 SEQ ID NO: 205 AAVhu.56 (AAV145.6) 308 US20150315612 SEQ ID NO: 168 AAVhu.56 (AAV145.6) 309 US20150315612 SEQ ID NO: 192 AAVhu.57 310 US20150315612 SEQ ID NO: 206 AAVhu.57 311 U520150315612 SEQ ID NO: 169 AAVhu.57 312 U520150315612 SEQ ID NO: 193 AAVhu.58 313 US20150315612 SEQ ID NO: 207 AAVhu.58 314 U520150315612 SEQ ID NO: 194 AAVhu.6 (AAV3.1) 315 US20150315612 SEQ ID NO: 5 AAVhu.6 (AAV3.1) 316 US20150315612 SEQ ID NO: 84 AAVhu.60 317 U520150315612 SEQ ID NO: 184 AAVhu.60 (AAV161.10) 318 U520150315612 SEQ ID NO: 170 AAVhu.61 319 U520150315612 SEQ ID NO: 185 AAVhu.61 (AAV161.6) 320 U520150315612 SEQ ID NO: 174 AAVhu.63 321 U520150315612 SEQ ID NO: 204 AAVhu.63 322 U520150315612 SEQ ID NO: 195 AAVhu.64 323 U520150315612 SEQ ID NO: 212 AAVhu.64 324 U520150315612 SEQ ID NO: 196 AAVhu.66 325 U520150315612 SEQ ID NO: 197 AAVhu.67 326 US20150315612 SEQ ID NO: 215 AAVhu.67 327 U520150315612 SEQ ID NO: 198 AAVhu.7 328 U520150315612 SEQ ID NO: 226 AAVhu.7 329 U520150315612 SEQ ID NO: 150 AAVhu.7 (AAV7.3) 330 US20150315612 SEQ ID NO: 55 AAVhu.71 331 U520150315612 SEQ ID NO: 79 AAVhu.8 332 US20150315612 SEQ ID NO: 53 AAVhu.8 333 U520150315612 SEQ ID NO: 12 AAVhu.8 334 U520150315612 SEQ ID NO: 151 AAVhu.9 (AAV3.1) 335 US20150315612 SEQ ID NO: 58 AAVhu.9 (AAV3.1) 336 U520150315612 SEQ ID NO: 155 AAV-LK01 337 U520150376607 SEQ ID NO: 2 AAV-LK01 338 U520150376607 SEQ ID NO: 29 AAV-LKO2 339 U520150376607 SEQ ID NO: 3 AAV-LKO2 340 U520150376607 SEQ ID NO: 30 AAV-LKO3 341 U520150376607 SEQ ID NO: 4 AAV-LKO3 342 WO2015121501 SEQ ID NO: 12, U520150376607 SEQ ID NO: 31 AAV-LKO4 343 U520150376607 SEQ ID NO: 5 AAV-LKO4 344 U520150376607 SEQ ID NO: 32 AAV-LKO5 345 U520150376607 SEQ ID NO: 6 AAV-LKO5 346 U520150376607 SEQ ID NO: 33 AAV-LKO6 347 U520150376607 SEQ ID NO: 7 AAV-LKO6 348 U520150376607 SEQ ID NO: 34 AAV-LKO7 349 U520150376607 SEQ ID NO: 8 AAV-LKO7 350 U520150376607 SEQ ID NO: 35 AAV-LKO8 351 U520150376607 SEQ ID NO: 9 AAV-LK08 352 US20150376607 SEQ ID NO: 36 AAV-LK09 353 US20150376607 SEQ ID NO: 10 AAV-LK09 354 US20150376607 SEQ ID NO: 37 AAV -LK10 355 US20150376607 SEQ ID NO: 11 AAV -LK10 356 U520150376607 SEQ ID NO: 38 AAV-LK11 357 U520150376607 SEQ ID NO: 12 AAV -LK11 358 U520150376607 SEQ ID NO: 39 AAV-LK12 359 U520150376607 SEQ ID NO: 13 AAV-LK12 360 U520150376607 SEQ ID NO: 40 AAV-LK13 361 U520150376607 SEQ ID NO: 14 AAV-LK13 362 U520150376607 SEQ ID NO: 41 AAV-LK14 363 U520150376607 SEQ ID NO: 15 AAV-LK14 364 U520150376607 SEQ ID NO: 42 AAV -LK15 365 U520150376607 SEQ ID NO: 16 AAV -LK15 366 U520150376607 SEQ ID NO: 43 AAV-LK16 367 U520150376607 SEQ ID NO: 17 AAV-LK16 368 U520150376607 SEQ ID NO: 44 AAV-LK17 369 U520150376607 SEQ ID NO: 18 AAV-LK17 370 U520150376607 SEQ ID NO: 45 AAV-LK18 371 U520150376607 SEQ ID NO: 19 AAV-LK18 372 US20150376607 SEQ ID NO: 46 AAV-LK19 373 U520150376607 SEQ ID NO: 20 AAV-LK19 374 U520150376607 SEQ ID NO: 47 AAV-PAEC 375 U520150376607 SEQ ID NO: 1 AAV-PAEC 376 U520150376607 SEQ ID NO: 48 AAV -PAEC11 377 US20150376607 SEQ ID NO: 26 AAV -PAEC11 378 US20150376607 SEQ ID NO: 54 AAV-PAEC12 379 U520150376607 SEQ ID NO: 27 AAV-PAEC12 380 U520150376607 SEQ ID NO: 51 AAV-PAEC13 381 U520150376607 SEQ ID NO: 28 AAV-PAEC13 382 U520150376607 SEQ ID NO: 49 AAV-PAEC2 383 U520150376607 SEQ ID NO: 21 AAV-PAEC2 384 U520150376607 SEQ ID NO: 56 AAV-PAEC4 385 U520150376607 SEQ ID NO: 22 AAV-PAEC4 386 U520150376607 SEQ ID NO: 55 AAV-PAEC6 387 U520150376607 SEQ ID NO: 23 AAV-PAEC6 388 U520150376607 SEQ ID NO: 52 AAV-PAEC7 389 U520150376607 SEQ ID NO: 24 AAV-PAEC7 390 US20150376607 SEQ ID NO: 53 AAV-PAEC8 391 US20150376607 SEQ ID NO: 25 AAV-PAEC8 392 U520150376607 SEQ ID NO: 50 AAVpi.1 393 U520150315612 SEQ ID NO: 28 AAVpi.1 394 U520150315612 SEQ ID NO: 93 AAVpi.2 395 U520150315612 SEQ ID NO: 30 AAVpi.2 396 U520150315612 SEQ ID NO: 95 AAVpi.3 397 U520150315612 SEQ ID NO: 29 AAVpi.3 398 U520150315612 SEQ ID NO: 94 AAVrh.10 399 U520150159173 SEQ ID NO: 9 AAVrh.10 400 U520150159173 SEQ ID NO: 25 AAV44.2 401 US20030138772 SEQ ID NO: 59 AAVrh.10 (AAV44.2) 402 U520030138772 SEQ ID NO: 81 AAV42.1B 403 U520030138772 SEQ ID NO: 90 AAVrh.12 (AAV42.1b) 404 U520030138772 SEQ ID NO: 30 AAVrh.13 405 U520150159173 SEQ ID NO: 10 AAVrh.13 406 U520150159173 SEQ ID NO: 26 AAVrh.13 407 US20150315612 SEQ ID NO: 228 AAVrh.13R 408 U520150159173 AAV42.3A 409 US20030138772 SEQ ID NO: 87 AAVrh.14 (AAV42.3a) 410 US20030138772 SEQ ID NO: 32 AAV42.5A 411 US20030138772 SEQ ID NO: 89 AAVrh.17 (AAV42.5a) 412 US20030138772 SEQ ID NO: 34 AAV42.5B 413 US20030138772 SEQ ID NO: 91 AAVrh.18 (AAV42.5b) 414 US20030138772 SEQ ID NO: 29 AAV42.6B 415 US20030138772 SEQ ID NO: 112 AAVrh.19 (AAV42.6b) 416 U520030138772 SEQ ID NO: 38 AAVrh.2 417 U520150159173 SEQ ID NO: 39 AAVrh.2 418 U520150315612 SEQ ID NO: 231 AAVrh.20 419 U520150159173 SEQ ID NO: 1 AAV42.10 420 U520030138772 SEQ ID NO: 106 AAVrh.21 (AAV42.10) 421 U520030138772 SEQ ID NO: 35 AAV42.11 422 U520030138772 SEQ ID NO: 108 AAVrh.22 (AAV42.11) 423 U520030138772 SEQ ID NO: 37 AAV42.12 424 U520030138772 SEQ ID NO: 113 AAVrh.23 (AAV42.12) 425 U520030138772 SEQ ID NO: 58 AAV42.13 426 U520030138772 SEQ ID NO: 86 AAVrh.24 (AAV42.13) 427 U520030138772 SEQ ID NO: 31 AAV42.15 428 U520030138772 SEQ ID NO: 84 AAVrh.25 (AAV42.15) 429 U520030138772 SEQ ID NO: 28 AAVrh.2R 430 U520150159173 AAVrh.31 (AAV223.1) 431 U520030138772 SEQ ID NO: 48 AAVC1 432 U520030138772 SEQ ID NO: 60 AAVrh.32 (AAVC1) 433 U520030138772 SEQ ID NO: 19 AAVrh.32/33 434 U520150159173 SEQ ID NO: 2 AAVrh.33 (AAVC3) 435 US20030138772 SEQ ID NO: 20 AAVC5 436 US20030138772 SEQ ID NO: 62 AAVrh.34 (AAVC5) 437 U520030138772 SEQ ID NO: 21 AAVF1 438 US20030138772 SEQ ID NO: 109 AAVrh.35 (AAVF1) 439 U520030138772 SEQ ID NO: 22 AAVF3 440 U520030138772 SEQ ID NO: 111 AAVrh.36 (AAVF3) 441 U520030138772 SEQ ID NO: 23 AAVrh.37 442 US20030138772 SEQ ID NO: 24 AAVrh.37 443 U520150159173 SEQ ID NO: 40 AAVrh.37 444 US20150315612 SEQ ID NO: 229 AAVrh.37R2 445 U520150159173 AAVrh.38 (AAVLG-4) 446 US20150315612 SEQ ID NO: 7 AAVrh.38 (AAVLG-4) 447 US20150315612 SEQ ID NO: 86 AAVrh.39 448 U520150159173 SEQ ID NO: 20, U520150315612 SEQ ID NO: 13 AAVrh.39 449 U520150159173 SEQ ID NO: 3, U520150159173 SEQ ID NO: 36, U520150315612 SEQ ID NO: 89 AAVrh.40 450 U520150315612 SEQ ID NO: 92 AAVrh.40 (AAVLG-10) 451 US20150315612 SEQ ID No: 14 AAVrh.43 (AAVN721-8) 452 U520150315612 SEQ ID NO: 43, U520150159173 SEQ ID NO: 21 AAVrh.43 (AAVN721-8) 453 U520150315612 SEQ ID NO: 163, U520150159173 SEQ ID NO: 37 AAVrh.44 454 U520150315612 SEQ ID NO: 34 AAVrh.44 455 U520150315612 SEQ ID NO: 111 AAVrh.45 456 U520150315612 SEQ ID NO: 41 AAVrh.45 457 U520150315612 SEQ ID NO: 109 AAVrh.46 458 US20150159173 SEQ ID NO: 22, US20150315612 SEQ ID NO: 19 AAVrh.46 459 US20150159173 SEQ ID NO: 4, US20150315612 SEQ ID NO: 101 AAVrh.47 460 U520150315612 SEQ ID NO: 38 AAVrh.47 461 U520150315612 SEQ ID NO: 118 AAVrh.48 462 U520150159173 SEQ ID NO: 44, U520150315612 SEQ ID NO: 115 AAVrh.48.1 463 US20150159173 AAVrh.48.1.2 464 US20150159173 AAVrh.48.2 465 US20150159173 AAVrh.48 (AAV1 -7) 466 US20150315612 SEQ ID NO: 32 AAVrh.49 (AAV1 -8) 467 US20150315612 SEQ ID NO: 25 AAVrh.49 (AAV1 -8) 468 US20150315612 SEQ ID NO: 103 AAVrh.50 (AAV2-4) 469 US20150315612 SEQ ID NO: 23 AAVrh.50 (AAV2-4) 470 US20150315612 SEQ ID NO: 108 AAVrh.51 (AAV2-5) 471 US20150315612 SEQ ID No: 22 AAVrh.51 (AAV2-5) 472 US20150315612 SEQ ID NO: 104 AAVrh.52 (AAV3-9) 473 US20150315612 SEQ ID NO: 18 AAVrh.52 (AAV3-9) 474 US20150315612 SEQ ID NO: 96 AAVrh.53 475 U520150315612 SEQ ID NO: 97 AAVrh.53 (AAV3-11) 476 U520150315612 SEQ ID NO: 17 AAVrh.53 (AAV3-11) 477 U520150315612 SEQ ID NO: 186 AAVrh.54 478 U520150315612 SEQ ID NO: 40 AAVrh.54 479 U520150159173 SEQ ID NO: 49, U520150315612 SEQ ID NO: 116 AAVrh.55 480 U520150315612 SEQ ID NO: 37 AAVrh.55 (AAV4-19) 481 U520150315612 SEQ ID NO: 117 AAVrh.56 482 U520150315612 SEQ ID NO: 54 AAVrh.56 483 U520150315612 SEQ ID NO: 152 AAVrh.57 484 U520150315612 SEQ ID NO: 26 AAVrh.57 485 U520150315612 SEQ ID NO: 105 AAVrh.58 486 U520150315612 SEQ ID NO: 27 AAVrh.58 487 U520150159173 SEQ ID NO: 48, U520150315612 SEQ ID NO: 106 AAVrh.58 488 U520150315612 SEQ ID NO: 232 AAVrh.59 489 U520150315612 SEQ ID NO: 42 AAVrh.59 490 U520150315612 SEQ ID NO: 110 AAVrh.60 491 US20150315612 SEQ ID NO: 31 AAVrh.60 492 U520150315612 SEQ ID NO: 120 AAVrh.61 493 U520150315612 SEQ ID NO: 107 AAVrh.61 (AAV2-3) 494 US20150315612 SEQ ID NO: 21 AAVrh.62 (AAV2-15) 495 U520150315612 SEQ ID No: 33 AAVrh.62 (AAV2-15) 496 U520150315612 SEQ ID NO: 114 AAVrh.64 497 U520150315612 SEQ ID No: 15 AAVrh.64 498 U520150159173 SEQ ID NO: 43, U520150315612 SEQ ID NO: 99 AAVrh.64 499 U520150315612 SEQ ID NO: 233 AAVRh.64R1 500 U520150159173 AAVRh.64R2 501 U520150159173 AAVrh.65 502 U520150315612 SEQ ID NO: 35 AAVrh.65 503 U520150315612 SEQ ID NO: 112 AAVrh.67 504 U520150315612 SEQ ID NO: 36 AAVrh.67 505 US20150315612 SEQ ID NO: 230 AAVrh.67 506 U520150159173 SEQ ID NO: 47, U520150315612 SEQ ID NO: 113 AAVrh.68 507 U520150315612 SEQ ID NO: 16 AAVrh.68 508 U520150315612 SEQ ID NO: 100 AAVrh.69 509 U520150315612 SEQ ID NO: 39 AAVrh.69 510 U520150315612 SEQ ID NO: 119 AAVrh.70 511 U520150315612 SEQ ID NO: 20 AAVrh.70 512 U520150315612 SEQ ID NO: 98 AAVrh.71 513 U520150315612 SEQ ID NO: 162 AAVrh.72 514 U520150315612 SEQ ID NO: 9 AAVrh.73 515 U520150159173 SEQ ID NO: 5 AAVrh.74 516 U520150159173 SEQ ID NO: 6 AAVrh.8 517 US20150159173 SEQ ID NO: 41 AAVrh.8 518 US20150315612 SEQ ID NO: 235 AAVrh.8R 519 US20150159173, WO2015168666 SEQ ID NO: 9 AAVrh.8R A586R mutant 520 WO2015168666 SEQ ID NO: 10 AAVrh.8R R533A mutant 521 WO2015168666 SEQ ID NO: 11 BAAV (bovine AAV) 522 US9193769 SEQ ID NO: 8 BAAV (bovine AAV) 523 US9193769 SEQ ID NO: 10 BAAV (bovine AAV) 524 US9193769 SEQ ID NO: 4 BAAV (bovine AAV) 525 US9193769 SEQ ID NO: 2 BAAV (bovine AAV) 526 US9193769 SEQ ID NO: 6 BAAV (bovine AAV) 527 US9193769 SEQ ID NO: 1 BAAV (bovine AAV) 528 US9193769 SEQ ID NO: 5 BAAV (bovine AAV) 529 US9193769 SEQ ID NO: 3 BAAV (bovine AAV) 530 US9193769 SEQ ID NO: 11 BAAV (bovine AAV) 531 U57427396 SEQ ID NO: 5 BAAV (bovine AAV) 532 U57427396 SEQ ID NO: 6 BAAV (bovine AAV) 533 US9193769 SEQ ID NO: 7 BAAV (bovine AAV) 534 US9193769 SEQ ID NO: 9 BNP61 AAV 535 US20150238550 SEQ ID NO: 1 BNP61 AAV 536 US20150238550 SEQ ID NO: 2 BNP62 AAV 537 US20150238550 SEQ ID NO: 3 BNP63 AAV 538 U520150238550 SEQ ID NO: 4 caprine AAV 539 U57427396 SEQ ID NO: 3 caprine AAV 540 U57427396 SEQ ID NO: 4 true type AAV (ttAAV) 541 WO2015121501 SEQ ID NO: 2 AAAV (Avian AAV) 542 U59238800 SEQ ID NO: 12 AAAV (Avian AAV) 543 U59238800 SEQ ID NO: 2 AAAV (Avian AAV) 544 U59238800 SEQ ID NO: 6 AAAV (Avian AAV) 545 U59238800 SEQ ID NO: 4 AAAV (Avian AAV) 546 U59238800 SEQ ID NO: 8 AAAV (Avian AAV) 547 U59238800 SEQ ID NO: 14 AAAV (Avian AAV) 548 U59238800 SEQ ID NO: 10 AAAV (Avian AAV) 549 U59238800 SEQ ID NO: 15 AAAV (Avian AAV) 550 U59238800 SEQ ID NO: 5 AAAV (Avian AAV) 551 U59238800 SEQ ID NO: 9 AAAV (Avian AAV) 552 U59238800 SEQ ID NO: 3 AAAV (Avian AAV) 553 U59238800 SEQ ID NO: 7 AAAV (Avian AAV) 554 U59238800 SEQ ID NO: 11 AAAV (Avian AAV) 555 U59238800 SEQ ID NO: 13 AAAV (Avian AAV) 556 U59238800 SEQ ID NO: 1 AAV Shuffle 100-1 557 U520160017295 SEQ ID NO: 23 AAV Shuffle 100-1 558 U520160017295 SEQ ID NO: 11 AAV Shuffle 100-2 559 U520160017295 SEQ ID NO: 37 AAV Shuffle 100-2 560 U520160017295 SEQ ID NO: 29 AAV Shuffle 100-3 561 U520160017295 SEQ ID NO: 24 AAV Shuffle 100-3 562 U520160017295 SEQ ID NO: 12 AAV Shuffle 100-7 563 U520160017295 SEQ ID NO: 25 AAV Shuffle 100-7 564 U520160017295 SEQ ID NO: 13 AAV Shuffle 10-2 565 U520160017295 SEQ ID NO: 34 AAV Shuffle 10-2 566 U520160017295 SEQ ID NO: 26 AAV Shuffle 10-6 567 U520160017295 SEQ ID NO: 35 AAV Shuffle 10-6 568 U520160017295 SEQ ID NO: 27 AAV Shuffle 10-8 569 U520160017295 SEQ ID NO: 36 AAV Shuffle 10-8 570 U520160017295 SEQ ID NO: 28 AAV SM 100-10 571 U520160017295 SEQ ID NO: 41 AAV SM 100-10 572 U520160017295 SEQ ID NO: 33 AAV SM 100-3 573 U520160017295 SEQ ID NO: 40 AAV SM 100-3 574 US20160017295 SEQ ID NO: 32 AAV SM 10-1 575 U520160017295 SEQ ID NO: 38 AAV SM 10-1 576 U520160017295 SEQ ID NO: 30 AAV SM 10-2 577 U520160017295 SEQ ID NO: 10 AAV SM 10-2 578 U520160017295 SEQ ID NO: 22 AAV SM 10-8 579 U520160017295 SEQ ID NO: 39 AAV SM 10-8 580 U520160017295 SEQ ID NO: 31 AAVF1/HSC1 581 WO2016049230 SEQ ID NO: 20 AAVF2/HSC2 582 WO2016049230 SEQ ID NO: 21 AAVF3/HSC3 583 WO2016049230 SEQ ID NO: 22 AAVF4/HSC4 584 WO2016049230 SEQ ID NO: 23 AAVF5/HSC5 585 WO2016049230 SEQ ID NO: 25 AAVF6/HSC6 586 WO2016049230 SEQ ID NO: 24 AAVF7/HSC7 587 WO2016049230 SEQ ID NO: 27 AAVF8/HSC8 588 WO2016049230 SEQ ID NO: 28 AAVF9/HSC9 589 WO2016049230 SEQ ID NO: 29 AAVF11/HSC11 590 WO2016049230 SEQ ID NO: 26 AAVF12/HSC12 591 WO2016049230 SEQ ID NO: 30 AAVF13/HSC13 592 WO2016049230 SEQ ID NO: 31 AAVF14/HSC14 593 WO2016049230 SEQ ID NO: 32 AAVF15/HSC15 594 WO2016049230 SEQ ID NO: 33 AAVF16/HSC16 595 WO2016049230 SEQ ID NO: 34 AAVF17/HSC17 596 WO2016049230 SEQ ID NO: 35 AAVF1/HSC1 597 WO2016049230 SEQ ID NO: 2 AAVF2/HSC2 598 WO2016049230 SEQ ID NO: 3 AAVF3/HSC3 599 WO2016049230 SEQ ID NO: 5 AAVF4/HSC4 600 WO2016049230 SEQ ID NO: 6 AAVF5/HSC5 601 WO2016049230 SEQ ID NO: 11 AAVF6/HSC6 602 WO2016049230 SEQ ID NO: 7 AAVF7/HSC7 603 WO2016049230 SEQ ID NO: 8 AAVF8/HSC8 604 WO2016049230 SEQ ID NO: 9 AAVF9/HSC9 605 WO2016049230 SEQ ID NO: 10 AAVF11/HSC11 606 WO2016049230 SEQ ID NO: 4 AAVF12/HSC12 607 WO2016049230 SEQ ID NO: 12 AAVF13/HSC13 608 WO2016049230 SEQ ID NO: 14 AAVF14/HSC14 609 WO2016049230 SEQ ID NO: 15 AAVF15/HSC15 610 WO2016049230 SEQ ID NO: 16 AAVF16/HSC16 611 WO2016049230 SEQ ID NO: 17 AAVF17/HSC17 612 WO2016049230 SEQ ID NO: 13 AAV CBr-E1 613 U58734809 SEQ ID NO: 13 AAV CBr-E2 614 U58734809 SEQ ID NO: 14 AAV CBr-E3 615 U58734809 SEQ ID NO: 15 AAV CBr-E4 616 U58734809 SEQ ID NO: 16 AAV CBr-E5 617 U58734809 SEQ ID NO: 17 AAV CBr-e5 618 U58734809 SEQ ID NO: 18 AAV CBr-E6 619 U58734809 SEQ ID NO: 19 AAV CBr-E7 620 U58734809 SEQ ID NO: 20 AAV CBr-E8 621 U58734809 SEQ ID NO: 21 AAV CLv-D1 622 U58734809 SEQ ID NO: 22 AAV CLv-D2 623 U58734809 SEQ ID NO: 23 AAV CLv-D3 624 U58734809 SEQ ID NO: 24 AAV CLv-D4 625 U58734809 SEQ ID NO: 25 AAV CLv-D5 626 U58734809 SEQ ID NO: 26 AAV CLv-D6 627 U58734809 SEQ ID NO: 27 AAV CLv-D7 628 U58734809 SEQ ID NO: 28 AAV CLv-D8 629 U58734809 SEQ ID NO: 29 AAV CLv-E1 630 U58734809 SEQ ID NO: 13 AAV CLv-R1 631 US8734809 SEQ ID NO: 30 AAV CLv-R2 632 US8734809 SEQ ID NO: 31 AAV CLv-R3 633 US8734809 SEQ ID NO: 32 AAV CLv-R4 634 US8734809 SEQ ID NO: 33 AAV CLv-R5 635 US8734809 SEQ ID NO: 34 AAV CLv-R6 636 US8734809 SEQ ID NO: 35 AAV CLv-R7 637 US8734809 SEQ ID NO: 36 AAV CLv-R8 638 U58734809 SEQ ID NO: 37 AAV CLv-R9 639 U58734809 SEQ ID NO: 38 AAV CLg-F1 640 U58734809 SEQ ID NO: 39 AAV CLg-F2 641 U58734809 SEQ ID NO: 40 AAV CLg-F3 642 U58734809 SEQ ID NO: 41 AAV CLg-F4 643 U58734809 SEQ ID NO: 42 AAV CLg-F5 644 U58734809 SEQ ID NO: 43 AAV CLg-F6 645 U58734809 SEQ ID NO: 43 AAV CLg-F7 646 U58734809 SEQ ID NO: 44 AAV CLg-F8 647 U58734809 SEQ ID NO: 43 AAV CSp-1 648 U58734809 SEQ ID NO: 45 AAV CSp-10 649 U58734809 SEQ ID NO: 46 AAV CSp-11 650 U58734809 SEQ ID NO: 47 AAV CSp-2 651 U58734809 SEQ ID NO: 48 AAV CSp-3 652 U58734809 SEQ ID NO: 49 AAV CSp-4 653 U58734809 SEQ ID NO: 50 AAV CSp-6 654 U58734809 SEQ ID NO: 51 AAV CSp-7 655 U58734809 SEQ ID NO: 52 AAV CSp-8 656 U58734809 SEQ ID NO: 53 AAV CSp-9 657 U58734809 SEQ ID NO: 54 AAV CHt-2 658 U58734809 SEQ ID NO: 55 AAV CHt-3 659 U58734809 SEQ ID NO: 56 AAV CKd-1 660 U58734809 SEQ ID NO: 57 AAV CKd-10 661 U58734809 SEQ ID NO: 58 AAV CKd-2 662 U58734809 SEQ ID NO: 59 AAV CKd-3 663 U58734809 SEQ ID NO: 60 AAV CKd-4 664 U58734809 SEQ ID NO: 61 AAV CKd-6 665 U58734809 SEQ ID NO: 62 AAV CKd-7 666 U58734809 SEQ ID NO: 63 AAV CKd-8 667 U58734809 SEQ ID NO: 64 AAV CLv-1 668 U58734809 SEQ ID NO: 65 AAV CLv-12 669 U58734809 SEQ ID NO: 66 AAV CLv-13 670 U58734809 SEQ ID NO: 67 AAV CLv-2 671 U58734809 SEQ ID NO: 68 AAV CLv-3 672 U58734809 SEQ ID NO: 69 AAV CLv-4 673 U58734809 SEQ ID NO: 70 AAV CLv-6 674 U58734809 SEQ ID NO: 71 AAV CLv-8 675 U58734809 SEQ ID NO: 72 AAV CKd-B1 676 U58734809 SEQ ID NO: 73 AAV CKd-B2 677 U58734809 SEQ ID NO: 74 AAV CKd-B3 678 U58734809 SEQ ID NO: 75 AAV CKd-B4 679 U58734809 SEQ ID NO: 76 AAV CKd-B5 680 U58734809 SEQ ID NO: 77 AAV CKd-B6 681 U58734809 SEQ ID NO: 78 AAV CKd-B7 682 U58734809 SEQ ID NO: 79 AAV CKd-B8 683 U58734809 SEQ ID NO: 80 AAV CKd-H1 684 U58734809 SEQ ID NO: 81 AAV CKd-H2 685 U58734809 SEQ ID NO: 82 AAV CKd-H3 686 U58734809 SEQ ID NO: 83 AAV CKd-H4 687 U58734809 SEQ ID NO: 84 AAV CKd-H5 688 US8734809 SEQ ID NO: 85 AAV CKd-H6 689 US8734809 SEQ ID NO: 77 AAV CHt-1 690 US8734809 SEQ ID NO: 86 AAV CLv1-1 691 US8734809 SEQ ID NO: 171 AAV CLv1-2 692 U58734809 SEQ ID NO: 172 AAV CLv1-3 693 U58734809 SEQ ID NO: 173 AAV CLv1-4 694 U58734809 SEQ ID NO: 174 AAV Clv1-7 695 U58734809 SEQ ID NO: 175 AAV Clv1-8 696 U58734809 SEQ ID NO: 176 AAV Clv1-9 697 U58734809 SEQ ID NO: 177 AAV Clv1-10 698 U58734809 SEQ ID NO: 178 AAV.VR-355 699 U58734809 SEQ ID NO: 181 AAV.hu.48R3 700 U58734809 SEQ ID NO: 183 AAV CBr-E1 701 U58734809 SEQ ID NO: 87 AAV CBr-E2 702 U58734809 SEQ ID NO: 88 AAV CBr-E3 703 U58734809 SEQ ID NO: 89 AAV CBr-E4 704 U58734809 SEQ ID NO: 90 AAV CBr-E5 705 U58734809 SEQ ID NO: 91 AAV CBr-e5 706 U58734809 SEQ ID NO: 92 AAV CBr-E6 707 U58734809 SEQ ID NO: 93 AAV CBr-E7 708 U58734809 SEQ ID NO: 94 AAV CBr-E8 709 U58734809 SEQ ID NO: 95 AAV CLv-D1 710 U58734809 SEQ ID NO: 96 AAV CLv-D2 711 U58734809 SEQ ID NO: 97 AAV CLv-D3 712 U58734809 SEQ ID NO: 98 AAV CLv-D4 713 U58734809 SEQ ID NO: 99 AAV CLv-D5 714 U58734809 SEQ ID NO: 100 AAV CLv-D6 715 U58734809 SEQ ID NO: 101 AAV CLv-D7 716 U58734809 SEQ ID NO: 102 AAV CLv-D8 717 U58734809 SEQ ID NO: 103 AAV CLv-E1 718 U58734809 SEQ ID NO: 87 AAV CLv-R1 719 U58734809 SEQ ID NO: 104 AAV CLv-R2 720 U58734809 SEQ ID NO: 105 AAV CLv-R3 721 U58734809 SEQ ID NO: 106 AAV CLv-R4 722 U58734809 SEQ ID NO: 107 AAV CLv-R5 723 U58734809 SEQ ID NO: 108 AAV CLv-R6 724 U58734809 SEQ ID NO: 109 AAV CLv-R7 725 U58734809 SEQ ID NO: 110 AAV CLv-R8 726 U58734809 SEQ ID NO: 111 AAV CLv-R9 727 U58734809 SEQ ID NO: 112 AAV CLg-F1 728 U58734809 SEQ ID NO: 113 AAV CLg-F2 729 U58734809 SEQ ID NO: 114 AAV CLg-F3 730 U58734809 SEQ ID NO: 115 AAV CLg-F4 731 U58734809 SEQ ID NO: 116 AAV CLg-F5 732 U58734809 SEQ ID NO: 117 AAV CLg-F6 733 U58734809 SEQ ID NO: 117 AAV CLg-F7 734 U58734809 SEQ ID NO: 118 AAV CLg-F8 735 U58734809 SEQ ID NO: 117 AAV CSp-1 736 U58734809 SEQ ID NO: 119 AAV CSp-10 737 U58734809 SEQ ID NO: 120 AAV CSp-11 738 U58734809 SEQ ID NO: 121 AAV CSp-2 739 U58734809 SEQ ID NO: 122 AAV CSp-3 740 U58734809 SEQ ID NO: 123 AAV CSp-4 741 U58734809 SEQ ID NO: 124 AAV CSp-6 742 U58734809 SEQ ID NO: 125 AAV CSp-7 743 U58734809 SEQ ID NO: 126 AAV CSp-8 744 U58734809 SEQ ID NO: 127 AAV CSp-9 745 US8734809 SEQ ID NO: 128 AAV CHt-2 746 US8734809 SEQ ID NO: 129 AAV CHt-3 747 US8734809 SEQ ID NO: 130 AAV CKd-1 748 US8734809 SEQ ID NO: 131 AAV CKd-10 749 US8734809 SEQ ID NO: 132 AAV CKd-2 750 US8734809 SEQ ID NO: 133 AAV CKd-3 751 US8734809 SEQ ID NO: 134 AAV CKd-4 752 U58734809 SEQ ID NO: 135 AAV CKd-6 753 U58734809 SEQ ID NO: 136 AAV CKd-7 754 U58734809 SEQ ID NO: 137 AAV CKd-8 755 U58734809 SEQ ID NO: 138 AAV CLv-1 756 U58734809 SEQ ID NO: 139 AAV CLv-12 757 U58734809 SEQ ID NO: 140 AAV CLv-13 758 U58734809 SEQ ID NO: 141 AAV CLv-2 759 U58734809 SEQ ID NO: 142 AAV CLv-3 760 U58734809 SEQ ID NO: 143 AAV CLv-4 761 U58734809 SEQ ID NO: 144 AAV CLv-6 762 U58734809 SEQ ID NO: 145 AAV CLv-8 763 U58734809 SEQ ID NO: 146 AAV CKd-B1 764 U58734809 SEQ ID NO: 147 AAV CKd-B2 765 U58734809 SEQ ID NO: 148 AAV CKd-B3 766 U58734809 SEQ ID NO: 149 AAV CKd-B4 767 U58734809 SEQ ID NO: 150 AAV CKd-B5 768 U58734809 SEQ ID NO: 151 AAV CKd-B6 769 U58734809 SEQ ID NO: 152 AAV CKd-B7 770 U58734809 SEQ ID NO: 153 AAV CKd-B8 771 U58734809 SEQ ID NO: 154 AAV CKd-H1 772 U58734809 SEQ ID NO: 155 AAV CKd-H2 773 U58734809 SEQ ID NO: 156 AAV CKd-H3 774 U58734809 SEQ ID NO: 157 AAV CKd-H4 775 U58734809 SEQ ID NO: 158 AAV CKd-H5 776 U58734809 SEQ ID NO: 159 AAV CKd-H6 777 U58734809 SEQ ID NO: 151 AAV CHt-1 778 U58734809 SEQ ID NO: 160 AAV CHt-P2 779 WO2016065001 SEQ ID NO: 1 AAV CHt-P5 780 WO2016065001 SEQ ID NO: 2 AAV CHt-P9 781 WO2016065001 SEQ ID NO: 3 AAV CBr-7.1 782 WO2016065001 SEQ ID NO: 4 AAV CBr-7.2 783 WO2016065001 SEQ ID NO: 5 AAV CBr-7.3 784 WO2016065001 SEQ ID NO: 6 AAV CBr-7.4 785 WO2016065001 SEQ ID NO: 7 AAV CBr-7.5 786 WO2016065001 SEQ ID NO: 8 AAV CBr-7.7 787 WO2016065001 SEQ ID NO: 9 AAV CBr-7.8 788 WO2016065001 SEQ ID NO: 10 AAV CBr-7.10 789 WO2016065001 SEQ ID NO: 11 AAV CKd-N3 790 WO2016065001 SEQ ID NO: 12 AAV CKd-N4 791 WO2016065001 SEQ ID NO: 13 AAV CKd-N9 792 WO2016065001 SEQ ID NO: 14 AAV CLv-L4 793 WO2016065001 SEQ ID NO: 15 AAV CLv-L5 794 WO2016065001 SEQ ID NO: 16 AAV CLv-L6 795 WO2016065001 SEQ ID NO: 17 AAV CLv-K1 796 WO2016065001 SEQ ID NO: 18 AAV CLv-K3 797 WO2016065001 SEQ ID NO: 19 AAV CLv-K6 798 WO2016065001 SEQ ID NO: 20 AAV CLv-M1 799 WO2016065001 SEQ ID NO: 21 AAV CLv-M11 800 WO2016065001 SEQ ID NO: 22 AAV CLv-M2 801 WO2016065001 SEQ ID NO: 23 AAV CLv-M5 802 WO2016065001 SEQ ID NO: 24 AAV CLv-M6 803 WO2016065001 SEQ ID NO: 25 AAV CLv-M7 804 WO2016065001 SEQ ID NO: 26 AAV CLv-M8 805 WO2016065001 SEQ ID NO: 27 AAV CLv-M9 806 WO2016065001 SEQ ID NO: 28 AAV CHt-P1 807 WO2016065001 SEQ ID NO: 29 AAV CHt-P6 808 WO2016065001 SEQ ID NO: 30 AAV CHt-P8 809 WO2016065001 SEQ ID NO: 31 AAV CHt-6.1 810 WO2016065001 SEQ ID NO: 32 AAV CHt-6.10 811 WO2016065001 SEQ ID NO: 33 AAV CHt-6.5 812 WO2016065001 SEQ ID NO: 34 AAV CHt-6.6 813 WO2016065001 SEQ ID NO: 35 AAV CHt-6.7 814 WO2016065001 SEQ ID NO: 36 AAV CHt-6.8 815 WO2016065001 SEQ ID NO: 37 AAV CSp-8.10 816 WO2016065001 SEQ ID NO: 38 AAV CSp-8.2 817 WO2016065001 SEQ ID NO: 39 AAV CSp-8.4 818 WO2016065001 SEQ ID NO: 40 AAV CSp-8.5 819 WO2016065001 SEQ ID NO: 41 AAV CSp-8.6 820 WO2016065001 SEQ ID NO: 42 AAV CSp-8.7 821 WO2016065001 SEQ ID NO: 43 AAV CSp-8.8 822 WO2016065001 SEQ ID NO: 44 AAV CSp-8.9 823 WO2016065001 SEQ ID NO: 45 AAV CBr-B7.3 824 WO2016065001 SEQ ID NO: 46 AAV CBr-B7.4 825 WO2016065001 SEQ ID NO: 47 AAV3B 826 WO2016065001 SEQ ID NO: 48 AAV4 827 WO2016065001 SEQ ID NO: 49 AAV5 828 WO2016065001 SEQ ID NO: 50 AAV CHt-P2 829 WO2016065001 SEQ ID NO: 51 AAV CHt-P5 830 WO2016065001 SEQ ID NO: 52 AAV CHt-P9 831 WO2016065001 SEQ ID NO: 53 AAV CBr-7.1 832 WO2016065001 SEQ ID NO: 54 AAV CBr-7.2 833 WO2016065001 SEQ ID NO: 55 AAV CBr-7.3 834 WO2016065001 SEQ ID NO: 56 AAV CBr-7.4 835 WO2016065001 SEQ ID NO: 57 AAV CBr-7.5 836 WO2016065001 SEQ ID NO: 58 AAV CBr-7.7 837 WO2016065001 SEQ ID NO: 59 AAV CBr-7.8 838 WO2016065001 SEQ ID NO: 60 AAV CBr-7.10 839 WO2016065001 SEQ ID NO: 61 AAV CKd-N3 840 WO2016065001 SEQ ID NO: 62 AAV CKd-N4 841 WO2016065001 SEQ ID NO: 63 AAV CKd-N9 842 WO2016065001 SEQ ID NO: 64 AAV CLv-L4 843 WO2016065001 SEQ ID NO: 65 AAV CLv-L5 844 WO2016065001 SEQ ID NO: 66 AAV CLv-L6 845 WO2016065001 SEQ ID NO: 67 AAV CLv-K1 846 WO2016065001 SEQ ID NO: 68 AAV CLv-K3 847 WO2016065001 SEQ ID NO: 69 AAV CLv-K6 848 WO2016065001 SEQ ID NO: 70 AAV CLv-M1 849 WO2016065001 SEQ ID NO: 71 AAV CLv-M11 850 WO2016065001 SEQ ID NO: 72 AAV CLv-M2 851 WO2016065001 SEQ ID NO: 73 AAV CLv-M5 852 WO2016065001 SEQ ID NO: 74 AAV CLv-M6 853 WO2016065001 SEQ ID NO: 75 AAV CLv-M7 854 WO2016065001 SEQ ID NO: 76 AAV CLv-M8 855 WO2016065001 SEQ ID NO: 77 AAV CLv-M9 856 WO2016065001 SEQ ID NO: 78 AAV CHt-P1 857 WO2016065001 SEQ ID NO: 79 AAV CHt-P6 858 WO2016065001 SEQ ID NO: 80 AAV CHt-P8 859 WO2016065001 SEQ ID NO: 81 AAV CHt-6.1 860 WO2016065001 SEQ ID NO: 82 AAV CHt-6.10 861 WO2016065001 SEQ ID NO: 83 AAV CHt-6.5 862 WO2016065001 SEQ ID NO: 84 AAV CHt-6.6 863 WO2016065001 SEQ ID NO: 85 AAV CHt-6.7 864 WO2016065001 SEQ ID NO: 86 AAV CHt-6.8 865 WO2016065001 SEQ ID NO: 87 AAV CSp-8.10 866 WO2016065001 SEQ ID NO: 88 AAV CSp-8.2 867 WO2016065001 SEQ ID NO: 89 AAV CSp-8.4 868 WO2016065001 SEQ ID NO: 90 AAV CSp-8.5 869 WO2016065001 SEQ ID NO: 91 AAV CSp-8.6 870 WO2016065001 SEQ ID NO: 92 AAV CSp-8.7 871 WO2016065001 SEQ ID NO: 93 AAV CSp-8.8 872 WO2016065001 SEQ ID NO: 94 AAV CSp-8.9 873 WO2016065001 SEQ ID NO: 95 AAV CBr-B7.3 874 WO2016065001 SEQ ID NO: 96 AAV CBr-B7.4 875 WO2016065001 SEQ ID NO: 97 AAV3B 876 WO2016065001 SEQ ID NO: 98 AAV4 877 WO2016065001 SEQ ID NO: 99 AAV5 878 WO2016065001 SEQ ID NO: 100 GPV 879 U59624274B2 SEQ ID NO: 192 B19 880 U59624274B2 SEQ ID NO: 193 MVM 881 U59624274B2 SEQ ID NO: 194 FPV 882 U59624274B2 SEQ ID NO: 195 CPV 883 U59624274B2 SEQ ID NO: 196 AAV6 884 U59546112B2 SEQ ID NO: 5 AAV6 885 U59457103B2 SEQ ID NO: 1 AAV2 886 U59457103B2 SEQ ID NO: 2 ShH10 887 U59457103B2 SEQ ID NO: 3 ShH13 888 U59457103B2 SEQ ID NO: 4 ShH10 889 U59457103B2 SEQ ID NO: 5 ShH10 890 U59457103B2 SEQ ID NO: 6 ShH10 891 U59457103B2 SEQ ID NO: 7 ShH10 892 U59457103B2 SEQ ID NO: 8 ShH10 893 U59457103B2 SEQ ID NO: 9 rh74 894 U59434928B2 SEQ ID NO: 1, U52015023924A1 SEQ ID NO: 2 rh74 895 U59434928B2 SEQ ID NO: 2, U52015023924A1 SEQ ID NO: 1 AAV8 896 U59434928B2 SEQ ID NO: 4 rh74 897 U59434928B2 SEQ ID NO: 5 rh74 (RHM4-1) 898 U52015023924A1 SEQ ID NO: 5, U520160375110A1 SEQ ID NO: 4 rh74 (RHM15-1) 899 U52015023924A1 SEQ ID NO: 6, U520160375110A1 SEQ ID NO: 5 rh74 (RHM15-2) 900 U52015023924A1 SEQ ID NO: 7, U520160375110A1 SEQ ID NO: 6 rh74 (RHM15-3/RHM15-5) 901 U52015023924A1 SEQ ID NO: 8, U520160375110A1 SEQ ID NO: 7 rh74 (RHM15-4) 902 U52015023924A1 SEQ ID NO: 9, U520160375110A1 SEQ ID NO: 8 rh74 (RHM15-6) 903 U52015023924A1 SEQ ID NO: 10, U520160375110A1 SEQ ID NO: 9 rh74 (RHM4-1) 904 U52015023924A1 SEQ ID NO: 11 rh74 (RHM15-1) 905 U52015023924A1 SEQ ID NO: 12 rh74 (RHM15-2) 906 U52015023924A1 SEQ ID NO: 13 rh74 (RHM15-3/RHM15-5) 907 U52015023924A1 SEQ ID NO: 14 rh74 (RHM15-4) 908 U52015023924A1 SEQ ID NO: 15 rh74 (RHM15-6) 909 U52015023924A1 SEQ ID NO: 16 AAV2 (comprising 910 US20160175389A1 SEQ ID NO: 9 lung specific polypeptide) AAV2 (comprising 911 US20160175389A1 SEQ ID NO: 10 lung specific polypeptide) Anc80 912 US20170051257A1 SEQ ID NO: 1 Anc80 913 U520170051257A1 SEQ ID NO: 2 Anc81 914 U520170051257A1 SEQ ID NO: 3 Anc80 915 U520170051257A1 SEQ ID NO: 4 Anc82 916 U520170051257A1 SEQ ID NO: 5 Anc82 917 U520170051257A1 SEQ ID NO: 6 Anc83 918 U520170051257A1 SEQ ID NO: 7 Anc83 919 U520170051257A1 SEQ ID NO: 8 Anc84 920 U520170051257A1 SEQ ID NO: 9 Anc84 921 U520170051257A1 SEQ ID NO: 10 Anc94 922 U520170051257A1 SEQ ID NO: 11 Anc94 923 U520170051257A1 SEQ ID NO: 12 Anc113 924 U520170051257A1 SEQ ID NO: 13 Anc113 925 U520170051257A1 SEQ ID NO: 14 Anc126 926 U520170051257A1 SEQ ID NO: 15 Anc126 927 U520170051257A1 SEQ ID NO: 16 Anc127 928 U520170051257A1 SEQ ID NO: 17 Anc127 929 U520170051257A1 SEQ ID NO: 18 Anc80L27 930 U520170051257A1 SEQ ID NO: 19 Anc80L59 931 U520170051257A1 SEQ ID NO: 20 Anc80L60 932 U520170051257A1 SEQ ID NO: 21 Anc80L62 933 U520170051257A1 SEQ ID NO: 22 Anc80L65 934 U520170051257A1 SEQ ID NO: 23 Anc80L33 935 U520170051257A1 SEQ ID NO: 24 Anc80L36 936 U520170051257A1 SEQ ID NO: 25 Anc80L44 937 U520170051257A1 SEQ ID NO: 26 Anc80L1 938 U520170051257A1 SEQ ID NO: 35 Anc80L1 939 U520170051257A1 SEQ ID NO: 36 AAV-X1 940 U58283151B2 SEQ ID NO: 11 AAV-Xlb 941 U58283151B2 SEQ ID NO: 12 AAV-X5 942 U58283151B2 SEQ ID NO: 13 AAV-X19 943 U58283151B2 SEQ ID NO: 14 AAV-X21 944 U58283151B2 SEQ ID NO: 15 AAV-X22 945 U58283151B2 SEQ ID NO: 16 AAV-X23 946 U58283151B2 SEQ ID NO: 17 AAV-X24 947 U58283151B2 SEQ ID NO: 18 AAV-X25 948 U58283151B2 SEQ ID NO: 19 AAV-X26 949 U58283151B2 SEQ ID NO: 20 AAV-X1 950 U58283151B2 SEQ ID NO: 21 AAV-Xlb 951 U58283151B2 SEQ ID NO: 22 AAV-X5 952 U58283151B2 SEQ ID NO: 23 AAV-X19 953 U58283151B2 SEQ ID NO: 24 AAV-X21 954 U58283151B2 SEQ ID NO: 25 AAV-X22 955 U58283151B2 SEQ ID NO: 26 AAV-X23 956 U58283151B2 SEQ ID NO: 27 AAV-X24 957 U58283151B2 SEQ ID NO: 28 AAV-X25 958 U58283151B2 SEQ ID NO: 29 AAV-X26 959 U58283151B2 SEQ ID NO: 30 AAVrh8 960 WO2016054554A1 SEQ ID NO: 8 AAVrh8VP2FC5 961 WO2016054554A1 SEQ ID NO: 9 AAVrh8VP2FC44 962 WO2016054554A1 SEQ ID NO: 10 AAVrh8VP2ApoB100 963 WO2016054554A1 SEQ ID NO: 11 AAVrh8VP2RVG 964 WO2016054554A1 SEQ ID NO: 12 AAVrh8VP2Angiopep-2 VP2 965 WO2016054554A1 SEQ ID NO: 13 AAV9.47VP1.3 966 WO2016054554A1 SEQ ID NO: 14 AAV9.47VP2ICAMg3 967 WO2016054554A1 SEQ ID NO: 15 AAV9.47VP2RVG 968 WO2016054554A1 SEQ ID NO: 16 AAV9.47VP2Angiopep-2 969 WO2016054554A1 SEQ ID NO: 17 AAV9.47VP2A-string 970 WO2016054554A1 SEQ ID NO: 18 AAVrh8VP2FC5 VP2 971 WO2016054554A1 SEQ ID NO: 19 AAVrh8VP2FC44 VP2 972 WO2016054554A1 SEQ ID NO: 20 AAVrh8VP2ApoB100 VP2 973 WO2016054554A1 SEQ ID NO: 21 AAVrh8VP2RVG VP2 974 WO2016054554A1 SEQ ID NO: 22 AAVrh8VP2Angiopep-2 VP2 975 WO2016054554A1 SEQ ID NO: 23 AAV9.47VP2ICAMg3 VP2 976 WO2016054554A1 SEQ ID NO: 24 AAV9.47VP2RVG VP2 977 WO2016054554A1 SEQ ID NO: 25 AAV9.47VP2Angiopep-2 VP2 978 WO2016054554A1 SEQ ID NO: 26 AAV9.47VP2A-string VP2 979 WO2016054554A1 SEQ ID NO: 27 rAAV-B1 980 WO2016054557A1 SEQ ID NO: 1 rAAV-B2 981 WO2016054557A1 SEQ ID NO: 2 rAAV-B3 982 WO2016054557A1 SEQ ID NO: 3 rAAV-B4 983 WO2016054557A1 SEQ ID NO: 4 rAAV-B1 984 WO2016054557A1 SEQ ID NO: 5 rAAV-B2 985 WO2016054557A1 SEQ ID NO: 6 rAAV-B3 986 WO2016054557A1 SEQ ID NO: 7 rAAV-B4 987 WO2016054557A1 SEQ ID NO: 8 rAAV-L1 988 WO2016054557A1 SEQ ID NO: 9 rAAV-L2 989 WO2016054557A1 SEQ ID NO: 10 rAAV-L3 990 WO2016054557A1 SEQ ID NO: 11 rAAV-L4 991 WO2016054557A1 SEQ ID NO: 12 rAAV-L1 992 WO2016054557A1 SEQ ID NO: 13 rAAV-L2 993 WO2016054557A1 SEQ ID NO: 14 rAAV-L3 994 WO2016054557A1 SEQ ID NO: 15 rAAV-L4 995 WO2016054557A1 SEQ ID NO: 16 AAV9 996 WO2016073739A1 SEQ ID NO: 3 rAAV 997 WO2016081811A1 SEQ ID NO: 1 rAAV 998 WO2016081811A1 SEQ ID NO: 2 rAAV 999 WO2016081811A1 SEQ ID NO: 3 rAAV 1000 WO2016081811A1 SEQ ID NO: 4 rAAV 1001 WO2016081811A1 SEQ ID NO: 5 rAAV 1002 WO2016081811A1 SEQ ID NO: 6 rAAV 1003 WO2016081811A1 SEQ ID NO: 7 rAAV 1004 WO2016081811A1 SEQ ID NO: 8 rAAV 1005 WO2016081811A1 SEQ ID NO: 9 rAAV 1006 WO2016081811A1 SEQ ID NO: 10 rAAV 1007 WO2016081811A1 SEQ ID NO: 11 rAAV 1008 WO2016081811A1 SEQ ID NO: 12 rAAV 1009 WO2016081811A1 SEQ ID NO: 13 rAAV 1010 WO2016081811A1 SEQ ID NO: 14 rAAV 1011 WO2016081811A1 SEQ ID NO: 15 rAAV 1012 WO2016081811A1 SEQ ID NO: 16 rAAV 1013 WO2016081811A1 SEQ ID NO: 17 rAAV 1014 WO2016081811A1 SEQ ID NO: 18 rAAV 1015 WO2016081811A1 SEQ ID NO: 19 rAAV 1016 WO2016081811A1 SEQ ID NO: 20 rAAV 1017 WO2016081811A1 SEQ ID NO: 21 rAAV 1018 WO2016081811A1 SEQ ID NO: 22 rAAV 1019 WO2016081811A1 SEQ ID NO: 23 rAAV 1020 WO2016081811A1 SEQ ID NO: 24 rAAV 1021 WO2016081811A1 SEQ ID NO: 25 rAAV 1022 WO2016081811A1 SEQ ID NO: 26 rAAV 1023 WO2016081811A1 SEQ ID NO: 27 rAAV 1024 WO2016081811A1 SEQ ID NO: 28 rAAV 1025 WO2016081811A1 SEQ ID NO: 29 rAAV 1026 WO2016081811A1 SEQ ID NO: 30 rAAV 1027 WO2016081811A1 SEQ ID NO: 31 rAAV 1028 WO2016081811A1 SEQ ID NO: 32 rAAV 1029 WO2016081811A1 SEQ ID NO: 33 rAAV 1030 WO2016081811A1 SEQ ID NO: 34 rAAV 1031 WO2016081811A1 SEQ ID NO: 35 rAAV 1032 WO2016081811A1 SEQ ID NO: 36 rAAV 1033 WO2016081811A1 SEQ ID NO: 37 rAAV 1034 WO2016081811A1 SEQ ID NO: 38 rAAV 1035 WO2016081811A1 SEQ ID NO: 39 rAAV 1036 WO2016081811A1 SEQ ID NO: 40 rAAV 1037 WO2016081811A1 SEQ ID NO: 41 rAAV 1038 WO2016081811A1 SEQ ID NO: 42 rAAV 1039 WO2016081811A1 SEQ ID NO: 43 rAAV 1040 WO2016081811A1 SEQ ID NO: 44 rAAV 1041 WO2016081811A1 SEQ ID NO: 45 rAAV 1042 WO2016081811A1 SEQ ID NO: 46 rAAV 1043 WO2016081811A1 SEQ ID NO: 47 rAAV 1044 WO2016081811A1 SEQ ID NO: 48 rAAV 1045 WO2016081811A1 SEQ ID NO: 49 rAAV 1046 WO2016081811A1 SEQ ID NO: 50 rAAV 1047 WO2016081811A1 SEQ ID NO: 51 rAAV 1048 WO2016081811A1 SEQ ID NO: 52 rAAV 1049 WO2016081811A1 SEQ ID NO: 53 rAAV 1050 WO2016081811A1 SEQ ID NO: 54 rAAV 1051 WO2016081811A1 SEQ ID NO: 55 rAAV 1052 WO2016081811A1 SEQ ID NO: 56 rAAV 1053 WO2016081811A1 SEQ ID NO: 57 rAAV 1054 WO2016081811A1 SEQ ID NO: 58 rAAV 1055 WO2016081811A1 SEQ ID NO: 59 rAAV 1056 WO2016081811A1 SEQ ID NO: 60 rAAV 1057 WO2016081811A1 SEQ ID NO: 61 rAAV 1058 WO2016081811A1 SEQ ID NO: 62 rAAV 1059 WO2016081811A1 SEQ ID NO: 63 rAAV 1060 WO2016081811A1 SEQ ID NO: 64 rAAV 1061 WO2016081811A1 SEQ ID NO: 65 rAAV 1062 WO2016081811A1 SEQ ID NO: 66 rAAV 1063 WO2016081811A1 SEQ ID NO: 67 rAAV 1064 WO2016081811A1 SEQ ID NO: 68 rAAV 1065 WO2016081811A1 SEQ ID NO: 69 rAAV 1066 WO2016081811A1 SEQ ID NO: 70 rAAV 1067 WO2016081811A1 SEQ ID NO: 71 rAAV 1068 WO2016081811A1 SEQ ID NO: 72 rAAV 1069 WO2016081811A1 SEQ ID NO: 73 rAAV 1070 WO2016081811A1 SEQ ID NO: 74 rAAV 1071 WO2016081811A1 SEQ ID NO: 75 rAAV 1072 WO2016081811A1 SEQ ID NO: 76 rAAV 1073 WO2016081811A1 SEQ ID NO: 77 rAAV 1074 WO2016081811A1 SEQ ID NO: 78 rAAV 1075 WO2016081811A1 SEQ ID NO: 79 rAAV 1076 WO2016081811A1 SEQ ID NO: 80 rAAV 1077 WO2016081811A1 SEQ ID NO: 81 rAAV 1078 WO2016081811A1 SEQ ID NO: 82 rAAV 1079 WO2016081811A1 SEQ ID NO: 83 rAAV 1080 WO2016081811A1 SEQ ID NO: 84 rAAV 1081 WO2016081811A1 SEQ ID NO: 85 rAAV 1082 WO2016081811A1 SEQ ID NO: 86 rAAV 1083 WO2016081811A1 SEQ ID NO: 87 rAAV 1084 WO2016081811A1 SEQ ID NO: 88 rAAV 1085 WO2016081811A1 SEQ ID NO: 89 rAAV 1086 WO2016081811A1 SEQ ID NO: 90 rAAV 1087 WO2016081811A1 SEQ ID NO: 91 rAAV 1088 WO2016081811A1 SEQ ID NO: 92 rAAV 1089 WO2016081811A1 SEQ ID NO: 93 rAAV 1090 WO2016081811A1 SEQ ID NO: 94 rAAV 1091 WO2016081811A1 SEQ ID NO: 95 rAAV 1092 WO2016081811A1 SEQ ID NO: 96 rAAV 1093 WO2016081811A1 SEQ ID NO: 97 rAAV 1094 WO2016081811A1 SEQ ID NO: 98 rAAV 1095 WO2016081811A1 SEQ ID NO: 99 rAAV 1096 WO2016081811A1 SEQ ID NO: 100 rAAV 1097 WO2016081811A1 SEQ ID NO: 101 rAAV 1098 WO2016081811A1 SEQ ID NO: 102 rAAV 1099 WO2016081811A1 SEQ ID NO: 103 rAAV 1100 WO2016081811A1 SEQ ID NO: 104 rAAV 1101 WO2016081811A1 SEQ ID NO: 105 rAAV 1102 WO2016081811A1 SEQ ID NO: 106 rAAV 1103 WO2016081811A1 SEQ ID NO: 107 rAAV 1104 WO2016081811A1 SEQ ID NO: 108 rAAV 1105 WO2016081811A1 SEQ ID NO: 109 rAAV 1106 WO2016081811A1 SEQ ID NO: 110 rAAV 1107 WO2016081811A1 SEQ ID NO: 111 rAAV 1108 WO2016081811A1 SEQ ID NO: 112 rAAV 1109 WO2016081811A1 SEQ ID NO: 113 rAAV 1110 WO2016081811A1 SEQ ID NO: 114 rAAV 1111 WO2016081811A1 SEQ ID NO: 115 rAAV 1112 WO2016081811A1 SEQ ID NO: 116 rAAV 1113 WO2016081811A1 SEQ ID NO: 117 rAAV 1114 WO2016081811A1 SEQ ID NO: 118 rAAV 1115 WO2016081811A1 SEQ ID NO: 119 rAAV 1116 WO2016081811A1 SEQ ID NO: 120 rAAV 1117 WO2016081811A1 SEQ ID NO: 121 rAAV 1118 WO2016081811A1 SEQ ID NO: 122 rAAV 1119 WO2016081811A1 SEQ ID NO: 123 rAAV 1120 WO2016081811A1 SEQ ID NO: 124 rAAV 1121 WO2016081811A1 SEQ ID NO: 125 rAAV 1122 WO2016081811A1 SEQ ID NO: 126 rAAV 1123 WO2016081811A1 SEQ ID NO: 127 rAAV 1124 WO2016081811A1 SEQ ID NO: 128 AAV8 E532K 1125 WO2016081811A1 SEQ ID NO: 133 AAV8 E532K 1126 WO2016081811A1 SEQ ID NO: 134 rAAV4 1127 WO2016115382A1 SEQ ID NO: 2 rAAV4 1128 WO2016115382A1 SEQ ID NO: 3 rAAV4 1129 WO2016115382A1 SEQ ID NO: 4 rAAV4 1130 WO2016115382A1 SEQ ID NO: 5 rAAV4 1131 WO2016115382A1 SEQ ID NO: 6 rAAV4 1132 WO2016115382A1 SEQ ID NO: 7 rAAV4 1133 WO2016115382A1 SEQ ID NO: 8 rAAV4 1134 WO2016115382A1 SEQ ID NO: 9 rAAV4 1135 WO2016115382A1 SEQ ID NO: 10 rAAV4 1136 WO2016115382A1 SEQ ID NO: 11 rAAV4 1137 WO2016115382A1 SEQ ID NO: 12 rAAV4 1138 WO2016115382A1 SEQ ID NO: 13 rAAV4 1139 WO2016115382A1 SEQ ID NO: 14 rAAV4 1140 WO2016115382A1 SEQ ID NO: 15 rAAV4 1141 WO2016115382A1 SEQ ID NO: 16 rAAV4 1142 WO2016115382A1 SEQ ID NO: 17 rAAV4 1143 WO2016115382A1 SEQ ID NO: 18 rAAV4 1144 WO2016115382A1 SEQ ID NO: 19 rAAV4 1145 WO2016115382A1 SEQ ID NO: 20 rAAV4 1146 WO2016115382A1 SEQ ID NO: 21 AAV11 1147 WO2016115382A1 SEQ ID NO: 22 AAV12 1148 WO2016115382A1 SEQ ID NO: 23 rh32 1149 WO2016115382A1 SEQ ID NO: 25 rh33 1150 WO2016115382A1 SEQ ID NO: 26 rh34 1151 WO2016115382A1 SEQ ID NO: 27 rAAV4 1152 WO2016115382A1 SEQ ID NO: 28 rAAV4 1153 WO2016115382A1 SEQ ID NO: 29 rAAV4 1154 WO2016115382A1 SEQ ID NO: 30 rAAV4 1155 WO2016115382A1 SEQ ID NO: 31 rAAV4 1156 WO2016115382A1 SEQ ID NO: 32 rAAV4 1157 WO2016115382A1 SEQ ID NO: 33 AAV2/8 1158 WO2016131981A1 SEQ ID NO: 47 AAV2/8 1159 WO2016131981A1 SEQ ID NO: 48 ancestral AAV 1160 WO2016154344A1 SEQ ID NO: 7 ancestral AAV variant C4 1161 WO2016154344A1 SEQ ID NO: 13 ancestral AAV variant C7 1162 WO2016154344A1 SEQ ID NO: 14 ancestral AAV variant G4 1163 WO2016154344A1 SEQ ID NO: 15 consensus amino acid sequence 1164 WO2016154344A1 SEQ ID NO: 16 of ancestral AAV variants, C4, C7 and G4 consensus amino acid sequence 1165 WO2016154344A1 SEQ ID NO: 17 of ancestral AAV variants, C4 and C7 AAV8 (with a AAV2 1166 WO2016150403A1 SEQ ID NO: 13 phospholipase domain) AAV VR-942n 1167 U520160289275A1 SEQ ID NO: 10 AAV5-A (M569V) 1168 U520160289275A1 SEQ ID NO: 13 AAV5-A (M569V) 1169 U520160289275A1 SEQ ID NO: 14 AAV5-A (Y585V) 1170 U520160289275A1 SEQ ID NO: 16 AAV5-A (Y585V) 1171 U520160289275A1 SEQ ID NO: 17 AAV5-A (L587T) 1172 U520160289275A1 SEQ ID NO: 19 AAV5-A (L587T) 1173 U520160289275A1 SEQ ID NO: 20 AAV5-A (Y585V/L587T) 1174 U520160289275A1 SEQ ID NO: 22 AAV5-A (Y585V/L587T) 1175 U520160289275A1 SEQ ID NO: 23 AAV5-B (D652A) 1176 U520160289275A1 SEQ ID NO: 25 AAV5-B (D652A) 1177 U520160289275A1 SEQ ID NO: 26 AAV5-B (T362M) 1178 U520160289275A1 SEQ ID NO: 28 AAV5-B (T362M) 1179 U520160289275A1 SEQ ID NO: 29 AAV5-B (Q359D) 1180 U520160289275A1 SEQ ID NO: 31 AAV5-B (Q359D) 1181 U520160289275A1 SEQ ID NO: 32 AAV5-B (E350Q) 1182 U520160289275A1 SEQ ID NO: 34 AAV5-B (E350Q) 1183 U520160289275A1 SEQ ID NO: 35 AAV5-B (P533S) 1184 U520160289275A1 SEQ ID NO: 37 AAV5-B (P533S) 1185 U520160289275A1 SEQ ID NO: 38 AAV5-B (P533G) 1186 U520160289275A1 SEQ ID NO: 40 AAV5-B (P533G) 1187 U520160289275A1 SEQ ID NO: 41 AAV5-mutation in loop VII 1188 US20160289275A1 SEQ ID NO: 43 AAV5-mutation in loop VII 1189 US20160289275A1 SEQ ID NO: 44 AAV8 1190 US20160289275A1 SEQ ID NO: 47 Mut A (LK03/AAV8) 1191 WO2016181123A1 SEQ ID NO: 1 Mut B (LK03/AAV5) 1192 WO2016181123A1 SEQ ID NO: 2 Mut C (AAV8/AAV3B) 1193 WO2016181123A1 SEQ ID NO: 3 Mut D (AAV5/AAV3B) 1194 WO2016181123A1 SEQ ID NO: 4 Mut E (AAV8/AAV3B) 1195 WO2016181123A1 SEQ ID NO: 5 Mut F (AAV3B/AAV8) 1196 WO2016181123A1 SEQ ID NO: 6 AAV44.9 1197 WO2016183297A1 SEQ ID NO: 4 AAV44.9 1198 WO2016183297A1 SEQ ID NO: 5 AAVrh8 1199 WO2016183297A1 SEQ ID NO: 6 AAV44.9 (5470N) 1200 WO2016183297A1 SEQ ID NO: 9 rh74 VP1 1201 U520160375110A1 SEQ ID NO: 1 AAV-LK03 (L125I) 1202 WO2017015102A1 SEQ ID NO: 5 AAV3B (S663V + T492V) 1203 WO2017015102A1 SEQ ID NO: 6 Anc80 1204 WO2017019994A2 SEQ ID NO: 1 Anc80 1205 WO2017019994A2 SEQ ID NO: 2 Anc81 1206 WO2017019994A2 SEQ ID NO: 3 Anc81 1207 WO2017019994A2 SEQ ID NO: 4 Anc82 1208 WO2017019994A2 SEQ ID NO: 5 Anc82 1209 WO2017019994A2 SEQ ID NO: 6 Anc83 1210 WO2017019994A2 SEQ ID NO: 7 Anc83 1211 WO2017019994A2 SEQ ID NO: 8 Anc84 1212 WO2017019994A2 SEQ ID NO: 9 Anc84 1213 WO2017019994A2 SEQ ID NO: 10 Anc94 1214 WO2017019994A2 SEQ ID NO: 11 Anc94 1215 WO2017019994A2 SEQ ID NO: 12 Anc113 1216 WO2017019994A2 SEQ ID NO: 13 Anc113 1217 WO2017019994A2 SEQ ID NO: 14 Anc126 1218 WO2017019994A2 SEQ ID NO: 15 Anc126 1219 WO2017019994A2 SEQ ID NO: 16 Anc127 1220 WO2017019994A2 SEQ ID NO: 17 Anc127 1221 WO2017019994A2 SEQ ID NO: 18 Anc80L27 1222 WO2017019994A2 SEQ ID NO: 19 Anc80L59 1223 WO2017019994A2 SEQ ID NO: 20 Anc80L60 1224 WO2017019994A2 SEQ ID NO: 21 Anc80L62 1225 WO2017019994A2 SEQ ID NO: 22 Anc80L65 1226 WO2017019994A2 SEQ ID NO: 23 Anc80L33 1227 WO2017019994A2 SEQ ID NO: 24 Anc80L36 1228 WO2017019994A2 SEQ ID NO: 25 Anc80L44 1229 WO2017019994A2 SEQ ID NO: 26 Anc80L1 1230 WO2017019994A2 SEQ ID NO: 35 Anc80L1 1231 WO2017019994A2 SEQ ID NO: 36 AAVrh10 1232 WO2017019994A2 SEQ ID NO: 41 Anc110 1233 WO2017019994A2 SEQ ID NO: 42 Anc110 1234 WO2017019994A2 SEQ ID NO: 43 AAVrh32.33 1235 WO2017019994A2 SEQ ID NO: 45 AAVrh74 1236 WO2017049031A1 SEQ ID NO: 1 AAV2 1237 WO2017053629A2 SEQ ID NO: 49 AAV2 1238 WO2017053629A2 SEQ ID NO: 50 AAV2 1239 WO2017053629A2 SEQ ID NO: 82 Parvo-like virus 1240 WO2017070476A2 SEQ ID NO: 1 Parvo-like virus 1241 WO2017070476A2 SEQ ID NO: 2 Parvo-like virus 1242 WO2017070476A2 SEQ ID NO: 3 Parvo-like virus 1243 WO2017070476A2 SEQ ID NO: 4 Parvo-like virus 1244 WO2017070476A2 SEQ ID NO: 5 Parvo-like virus 1245 WO2017070476A2 SEQ ID NO: 6 AAVrh.10 1246 WO2017070516A1 SEQ ID NO: 7 AAVrh.10 1247 WO2017070516A1 SEQ ID NO: 14 AAV2tYF 1248 WO2017070491A1 SEQ ID NO: 1 AAV-SPK 1249 WO2017075619A1 SEQ ID NO:28 AAV2.5 1250 U520170128528A1 SEQ ID NO: 13 AAV1.1 1251 U520170128528A1 SEQ ID NO: 15 AAV6.1 1252 U520170128528A1 SEQ ID NO: 17 AAV6.3.1 1253 U520170128528A1 SEQ ID NO: 18 AAV2i8 1254 U520170128528A1 SEQ ID NO: 28 AAV2i8 1255 U520170128528A1 SEQ ID NO: 29 ttAAV 1256 U520170128528A1 SEQ ID NO: 30 ttAAV-5312N 1257 U520170128528A1 SEQ ID NO: 32 ttAAV-5312N 1258 U520170128528A1 SEQ ID NO: 33 AAV6 (Y705, Y731, and T492) 1259 WO2016134337A1 SEQ ID NO: 24 AAV2 1260 WO2016134375A1 SEQ ID NO: 9 AAV2 1261 WO2016134375A1 SEQ ID NO: 10

Each of the patents, applications and or publications listed in Table 6 are hereby incorporated by reference in their entirety.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence as described in International Patent Publication WO2015038958, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAV9 (SEQ ID NO: 2 and 11 of WO2015038958 or SEQ ID NO: 138 and 137 respectively herein), PHP.B (SEQ ID NO: 8 and 9 of WO2015038958, herein SEQ ID NO: 5 and 6), G2B-13 (SEQ ID NO: 12 of WO2015038958, herein SEQ ID NO: 7), G2B-26 (SEQ ID NO: 13 of WO2015038958, herein SEQ ID NO: 5), TH1.1-32 (SEQ ID NO: 14 of WO2015038958, herein SEQ ID NO: 8), TH1.1-35 (SEQ ID NO: 15 of WO2015038958, herein SEQ ID NO: 9) or variants thereof.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid described herein does not comprise an insert sequence present immediately subsequent to position 586, 588, or 589 numbered relative to SEQ ID NO: 138, having at least 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, of any of the following amino acid sequences, TLAVPFK (SEQ ID NO: 1262), KFPVALT (SEQ ID NO: 1263), LAVPFK (SEQ ID NO: 1264), AVPFK (SEQ ID NO: 1265), VPFK (SEQ ID NO: 1266), TLAVPF (SEQ ID NO: 1267), TLAVP (SEQ ID NO: 1268), TLAV (SEQ ID NO: 1269), SVSKPFL (SEQ ID NO: 1270), FTLTTPK (SEQ ID NO: 1271), MNATKNV (SEQ ID NO: 1272), QSSQTPR (SEQ ID NO: 1273), ILGTGTS (SEQ ID NO: 1274), TRTNPEA (SEQ ID NO: 1275), NGGTSSS (SEQ ID NO: 1276), or YTLSQGW (SEQ ID NO: 1277); or encoded by a nucleotide sequence of any of SEQ ID NO: 1278, SEQ ID NO: 1279, SEQ ID NO: 1280, SEQ ID NO: 1281, SEQ ID NO: 1282, SEQ ID NO: 1283, SEQ ID NO: 1284, SEQ ID NO: 1285, SEQ ID NO: 1286, or SEQ ID NO: 1287.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence as described in International Patent Publication WO2017100671, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAV9 (SEQ ID NO: 11), PHP.N (SEQ ID NO: 4), PHP.S (SEQ ID NO: 10), or variants thereof.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid described herein does not comprise an insert sequence present immediately subsequent to position 586, 588, or 589 numbered relative to SEQ ID NO: 138, having at least 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, of the targeting peptides or amino acid inserts described in WO2017100671.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid described herein does not comprise an insert sequence present immediately subsequent to position 586, 588, or 589 numbered relative to SEQ ID NO: 138, having at least 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, of any of the following amino acid sequences, AQTLAVPFKAQ (SEQ ID NO: 1288), AQSVSKPFLAQ (SEQ ID NO: 1289), AQFTLTTPKAQ (SEQ ID NO: 1290), DGTLAVPFKAQ (SEQ ID NO: 1291), ESTLAVPFKAQ (SEQ ID NO: 1292), GGTLAVPFKAQ (SEQ ID NO: 1293), AQTLATPFKAQ (SEQ ID NO: 1294), ATTLATPFKAQ (SEQ ID NO: 1295), DGTLATPFKAQ (SEQ ID NO: 1296), GGTLATPFKAQ (SEQ ID NO: 1297), SGSLAVPFKAQ (SEQ ID NO: 1298), AQTLAQPFKAQ (SEQ ID NO: 1299), AQTLQQPFKAQ (SEQ ID NO: 1300), AQTLSNPFKAQ (SEQ ID NO: 1301), AQTLAVPFSNP (SEQ ID NO: 1302), QGTLAVPFKAQ (SEQ ID NO: 1303), NQTLAVPFKAQ (SEQ ID NO: 1304), EGSLAVPFKAQ (SEQ ID NO: 1305), SGNLAVPFKAQ (SEQ ID NO: 1306), EGTLAVPFKAQ (SEQ ID NO: 1307), DSTLAVPFKAQ (SEQ ID NO: 1308), AVTLAVPFKAQ (SEQ ID NO: 1309), AQTLSTPFKAQ (SEQ ID NO: 1310), AQTLPQPFKAQ (SEQ ID NO: 1311), AQTLSQPFKAQ (SEQ ID NO: 1312), AQTLQLPFKAQ (SEQ ID NO: 1313), AQTLTMPFKAQ (SEQ ID NO: 1314), AQTLTTPFKAQ (SEQ ID NO: 1315), AQYTLSQGWAQ (SEQ ID NO: 1316), AQMNATKNVAQ (SEQ ID NO: 1317), AQVSGGHHSAQ (SEQ ID NO: 1318), AQTLTAPFKAQ (SEQ ID NO: 1319), AQTLSKPFKAQ (SEQ ID NO: 1320), QAVRTSL (SEQ ID NO: 1321), YTLSQGW (SEQ ID NO: 1277), LAKERLS (SEQ ID NO: 1322), TLAVPFK (SEQ ID NO: 1262), SVSKPFL (SEQ ID NO: 1270), FTLTTPK (SEQ ID NO: 1271), MNSTKNV (SEQ ID NO: 1323), VSGGHHS (SEQ ID NO: 1324), SAQTLAVPFKAQAQ (SEQ ID NO: 1325), SXXXLAVPFKAQAQ (wherein X may be any amino acid; SEQ ID NO: 1326), SAQXXXVPFKAQAQ (wherein X may be any amino acid; SEQ ID NO: 1327), SAQTLXXXFKAQAQ (wherein X may be any amino acid; SEQ ID NO: 1328), SAQTLAVXXXAQAQ (wherein X may be any amino acid; SEQ ID NO: 1329), SAQTLAVPFXXXAQ (wherein X may be any amino acid; SEQ ID NO: 1330), TNHQSAQ (SEQ ID NO: 1331), AQAQTGW (SEQ ID NO: 1332), DGTLATPFK (SEQ ID NO: 1333), DGTLATPFKXX (wherein X may be any amino acid; SEQ ID NO: 1334), LAVPFKAQ (SEQ ID NO: 1335), VPFKAQ (SEQ ID NO: 1336), FKAQ (SEQ ID NO: 1337), AQTLAV (SEQ ID NO: 1338), AQTLAVPF (SEQ ID NO: 1339), QAVR (SEQ ID NO: 1340), AVRT (SEQ ID NO: 1341), VRTS (SEQ ID NO: 1342), RTSL (SEQ ID NO: 1343), QAVRT (SEQ ID NO: 1344), AVRTS (SEQ ID NO: 1345), VRTSL (SEQ ID NO: 1346), QAVRTS (SEQ ID NO: 1347), or AVRTSL (SEQ ID NO: 1348); or encoded by a nucleotide sequence of any of SEQ ID NO: 1349, SEQ ID NO: 1350, SEQ ID NO: 1351, SEQ ID NO: 1352, SEQ ID NO: 1353, SEQ ID NO: 1354, SEQ ID NO: 1355, SEQ ID NO: 1356, SEQ ID NO: 1357, SEQ ID NO: 1358 (wherein N may be A, C, T, or G), SEQ ID NO: 1359 (wherein N may be A, C, T, or G), SEQ ID NO: 1360 (wherein N may be A, C, T, or G), SEQ ID NO: 1361 (wherein N may be A, C, T, or G), SEQ ID NO: 1362 (wherein N may be A, C, T, or G), SEQ ID NO: 1279, SEQ ID NO: 1280, SEQ ID NO: 1281, SEQ ID NO: 1287, or SEQ ID NO: 1363.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence as described in U.S. Pat. No. 9,624,274, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAV1 (SEQ ID NO: 181 of U.S. Pat. No. 9,624,274), AAV6 (SEQ ID NO: 182 of U.S. Pat. No. 9,624,274), AAV2 (SEQ ID NO: 183 of U.S. Pat. No. 9,624,274), AAV3b (SEQ ID NO: 184 of U.S. Pat. No. 9,624,274), AAV7 (SEQ ID NO: 185 of U.S. Pat. No. 9,624,274), AAV8 (SEQ ID NO: 186 of U.S. Pat. No. 9,624,274), AAV10 (SEQ ID NO: 187 of U.S. Pat. No. 9,624,274), AAV4 (SEQ ID NO: 188 of U.S. Pat. No. 9,624,274), AAV11 (SEQ ID NO: 189 of U.S. Pat. No. 9,624,274), bAAV (SEQ ID NO: 190 of U.S. Pat. No. 9,624,274), AAV5 (SEQ ID NO: 191 of U.S. Pat. No. 9,624,274), GPV (SEQ ID NO: 192 of U.S. Pat. No. 9,624,274; herein SEQ ID NO: 879), B19 (SEQ ID NO: 193 of U.S. Pat. No. 9,624,274; herein SEQ ID NO: 880), MVM (SEQ ID NO: 194 of U.S. Pat. No. 9,624,274; herein SEQ ID NO: 881), FPV (SEQ ID NO: 195 of U.S. Pat. No. 9,624,274; herein SEQ ID NO: 882), CPV (SEQ ID NO: 196 of U.S. Pat. No. 9,624,274; herein SEQ ID NO: 883) or variants thereof.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid described herein does not comprise an insert sequence present immediately subsequent to position 586, 588, or 589 numbered relative to SEQ ID NO: 138, having at least 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, of any of the structural protein inserts described in U.S. Pat. No. 9,624,274 or any of the following amino acid sequences, VNLTWSRASG (SEQ ID NO: 1364), EFCINHRGYWVCGD (SEQ ID NO: 1365), EDGQVMDVDLS (SEQ ID NO: 1366), EKQRNGTLT (SEQ ID NO: 1367), TYQCRVTHPHLPRALMR (SEQ ID NO: 1368), RHSTTQPRKTKGSG (SEQ ID NO: 1369), DSNPRGVSAYLSR (SEQ ID NO: 1370), TITCLWDLAPSK (SEQ ID NO: 1371), KTKGSGFFVF (SEQ ID NO: 1372), THPHLPRALMRS (SEQ ID NO: 1373), GETYQCRVTHPHLPRALMRSTTK (SEQ ID NO: 1374), LPRALMRS (SEQ ID NO: 1375), INHRGYWV (SEQ ID NO: 1376), CDAGSVRTNAPD (SEQ ID NO: 1377), AKAVSNLTESRSESLQS (SEQ ID NO: 1378), SLTGDEFKKVLET (SEQ ID NO: 1379), REAVAYRFEED (SEQ ID NO: 1380), INPEIITLDG (SEQ ID NO: 1381), DISVTGAPVITATYL (SEQ ID NO: 1382), DISVTGAPVITA (SEQ ID NO: 1383), PKTVSNLTESSSESVQS (SEQ ID NO: 1384), SLMGDEFKAVLET (SEQ ID NO: 1385), QHSVAYTFEED (SEQ ID NO: 1386), INPEIITRDG (SEQ ID NO: 1387), DISLTGDPVITASYL (SEQ ID NO: 1388), DISLTGDPVITA (SEQ ID NO: 1389), DQSIDFEIDSA (SEQ ID NO: 1390), KNVSEDLPLPTFSPTLLGDS (SEQ ID NO: 1391), KNVSEDLPLPT (SEQ ID NO: 1392), CDSGRVRTDAPD (SEQ ID NO: 1393), FPEHLLVDFLQSLS (SEQ ID NO: 1394), DAEFRHDSG (SEQ ID NO: 1395), HYAAAQWDFGNTMCQL (SEQ ID NO: 1396), YAAQWDFGNTMCQ (SEQ ID NO: 1397), RSQKEGLHYT (SEQ ID NO: 1398), SSRTPSDKPVAHWANPQAE (SEQ ID NO: 1399), SRTPSDKPVAHWANP (SEQ ID NO: 1400), SSRTPSDKP (SEQ ID NO: 1401), NADGNVDYHMNSVP (SEQ ID NO: 1402), DGNVDYHMNSV (SEQ ID NO: 1403), RSFKEFLQSSLRALRQ (SEQ ID NO: 1404); FKEFLQSSLRA (SEQ ID NO: 1405), or QMWAPQWGPD (SEQ ID NO: 1406).

In some embodiments, the AAV serotype, the parent AAV capsid polypeptide, or the AAV capsid variant may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence as described in U.S. Pat. No. 9,475,845, the contents of which are herein incorporated by reference in their entirety.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid described herein does not comprise an insert sequence present immediately subsequent to position 586, 588, or 589 numbered relative to SEQ ID NO: 138, having at least 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, of any of the amino acid sequences of RGNRQA (SEQ ID NO: 1407), SSSTDP (SEQ ID NO: 1408), SSNTAP (SEQ ID NO: 1409), SNSNLP (SEQ ID NO: 1410), SSTTAP (SEQ ID NO: 1411), AANTAA (SEQ ID NO: 1412), QQNTAP (SEQ ID NO: 1413), SAQAQA (n SEQ ID NO: 1414), QANTGP (SEQ ID NO: 1415), NATTAP (SEQ ID NO: 1416), SSTAGP (SEQ ID NO: 1417), QQNTAA (SEQ ID NO: 1418), PSTAGP (SEQ ID NO: 1419), NQNTAP (SEQ ID NO: 1420), QAANAP (SEQ ID NO: 1421), SIVGLP (SEQ ID NO: 1422), AASTAA (SEQ ID NO: 1423), SQNTTA (SEQ ID NO: 1424), QQDTAP (SEQ ID NO: 1425), QTNTGP (SEQ ID NO: 1426), QTNGAP (SEQ ID NO: 1427), QQNAAP (SEQ ID NO: 1428), or AANTQA (SEQ ID NO: 1429).

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid described herein may further comprise an amino acid modification, e.g., a substitution or insertion, at amino acid positions 262 through 265, numbered according to the AAV2 capsid protein or the corresponding position in the capsid protein of another AAV with a targeting sequence, e.g., of any of the amino acid sequences of, NGRAHA (SEQ ID NO: 1430), QPEHSST (SEQ ID NO: 1431), VNTANST (SEQ ID NO: 1432), HGPMQKS (SEQ ID NO: 1433), PHKPPLA (SEQ ID NO: 1434), IKNNEMW (SEQ ID NO: 1435), RNLDTPM (SEQ ID NO: 1436), VDSHRQS (SEQ ID NO: 1437), YDSKTKT (SEQ ID NO: 1438), SQLPHQK (SEQ ID NO: 1439), STMQQNT (SEQ ID NO: 1440), TERYMTQ (SEQ ID NO: 1441), DASLSTS (SEQ ID NO: 1442), DLPNKKT (SEQ ID NO: 1443), DLTAARL (SEQ ID NO: 1444), EPHQFNY (SEQ ID NO: 1445), EPQSNHT (SEQ ID NO: 1446), MSSWPSQ (SEQ ID NO: 1447), NPKHNAT (SEQ ID NO: 1448), PDGMRTT (SEQ ID NO: 1449), PNNNKTT (SEQ ID NO: 1450), QSTTHDS (SEQ ID NO: 1451), TGSKQKQ (SEQ ID NO: 1452), SLKHQAL (SEQ ID NO: 1453), SPIDGEQ (SEQ ID NO: 1454), WIFPWIQL (SEQ ID NO: 1455), CDCRGDCFC (SEQ ID NO: 1456), CNGRC (SEQ ID NO: 1457), CPRECES (SEQ ID NO: 1458), CTTHWGFTLC (SEQ ID NO: 1459), CGRRAGGSC (SEQ ID NO: 1460), CKGGRAKDC (SEQ ID NO: 1461), CVPELGHEC (SEQ ID NO: 1462), CRRETAWAK (SEQ ID NO: 1463), VSWFSHRYSPFAVS (SEQ ID NO: 1464), GYRDGYAGPILYN (SEQ ID NO: 1465), XXXYXXX (SEQ ID NO: 1466), YXNW (SEQ ID NO: 1467), RPLPPLP (SEQ ID NO: 1468), APPLPPR (SEQ ID NO: 1469), DVFYPYPYASGS (SEQ ID NO: 1470), MYWYPY (SEQ ID NO: 1471), DITWDQLWDLMK (SEQ ID NO: 1472), CWDDXWLC (SEQ ID NO: 1473), EWCEYLGGYLRCYA (SEQ ID NO: 1474), YXCXXGPXTWXCXP (SEQ ID NO: 1475), IEGPTLRQWLAARA (SEQ ID NO: 1476), LWXXX (SEQ ID NO: 1477), XFXXYLW (SEQ ID NO: 1478), SSIISHFRWGLCD (SEQ ID NO: 1479), MSRPACPPNDKYE (SEQ ID NO: 1480), CLRSGRGC (SEQ ID NO: 1481), CHWMFSPWC (SEQ ID NO: 1482), WXXF (SEQ ID NO: 1483), CSSRLDAC (SEQ ID NO: 1484), CLPVASC (SEQ ID NO: 1485), CGFECVRQCPERC (SEQ ID NO: 1486), CVALCREACGEGC (SEQ ID NO: 1487), SWCEPGWCR (SEQ ID NO: 1488), YSGKWGW (SEQ ID NO: 1489), GLSGGRS (SEQ ID NO: 1490), LMLPRAD (SEQ ID NO: 1491), CSCFRDVCC (SEQ ID NO: 1492), CRDVVSVIC (SEQ ID NO: 1493), MARSGL (SEQ ID NO: 1494), MARAKE (SEQ ID NO: 1495), MSRTMS (SEQ ID NO: 1496, KCCYSL (SEQ ID NO: 1497), MYWGDSHWLQYWYE (SEQ ID NO: 1498), MQLPLAT (SEQ ID NO: 1499), EWLS (SEQ ID NO: 1500), SNEW (SEQ ID NO: 1501), TNYL (SEQ ID NO: 1502), WDLAWMFRLPVG (SEQ ID NO: 1503), CTVALPGGYVRVC (SEQ ID NO: 1504), CVAYCIEHHCWTC (SEQ ID NO: 1505), CVFAHNYDYLVC (SEQ ID NO: 1506), CVFTSNYAFC (SEQ ID NO: 1507), VHSPNKK (SEQ ID NO: 1508), CRGDGWC (SEQ ID NO: 1509), XRGCDX (SEQ ID NO: 1510), PXXX (SEQ ID NO: 1511), SGKGPRQITAL (SEQ ID NO: 1512), AAAAAAAAAXXXXX (SEQ ID NO: 1513), VYMSPF (SEQ ID NO: 1514), ATWLPPR (SEQ ID NO: 1515), HTMYYHHYQHHL (SEQ ID NO: 1516), SEVGCRAGPLQWLCEKYFG (SEQ ID NO: 1517), CGLLPVGRPDRNVWRWLC (SEQ ID NO: 1518), CKGQCDRFKGLPWEC (SEQ ID NO: 1519), SGRSA (SEQ ID NO: 1520), WGFP (SEQ ID NO: 1521), AEPMPHSLNFSQYLWYT (SEQ ID NO: 1522), WAYXSP (SEQ ID NO: 1523), IELLQAR (SEQ ID NO: 1524), AYTKCSRQWRTCMTTH (SEQ ID NO: 1525), PQNSKIPGPTFLDPH (SEQ ID NO: 1526), SMEPALPDWWWKMFK (SEQ ID NO: 1527), ANTPCGPYTHDCPVKR (SEQ ID NO: 1528), TACHQHVRMVRP (SEQ ID NO: 1529), VPWMEPAYQRFL (SEQ ID NO: 1530), DPRATPGS (SEQ ID NO: 1531), FRPNRAQDYNTN (SEQ ID NO: 1532), CTKNSYLMC (SEQ ID NO: 1533), CXXTXXXGXGC (SEQ ID NO: 1534), CPIEDRPMC (SEQ ID NO: 1535), HEWSYLAPYPWF (SEQ ID NO: 1536), MCPKHPLGC (SEQ ID NO: 1537), RMWPSSTVNLSAGRR (SEQ ID NO: 1538), SAKTAVSQRVWLPSHRGGEP (SEQ ID NO: 1539), KSREHVNNSACPSKRITAAL (SEQ ID NO: 1540), EGFR (SEQ ID NO: 1541), AGLGVR SEQ ID NO: 1542), GTRQGHTMRLGVSDG (SEQ ID NO: 1543), IAGLATPGWSHWLAL (SEQ ID NO: 1544), SMSIARL (SEQ ID NO: 1545), HTFEPGV (SEQ ID NO: 1546), NTSLKRISNKRIRRK (SEQ ID NO: 1547), LRIKRKRRKRKKTRK (SEQ ID NO: 1548), GGG, GFS, LWS, EGG, LLV, LSP, LBS, AGG, GRR, GGH and GTV.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be or have, at a position other than 5 consecutive amino acids corresponding to positions 586 to 594 numbered relative to SEQ ID NO: 138, a sequence as described in United States Publication No. US 20160369298, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, site-specific mutated capsid protein of AAV2 (SEQ ID NO: 97 of US 20160369298; herein SEQ ID NO: 1549) or variants thereof, wherein the specific site is at least one site selected from sites R447, G453, and/or 5662 of a VP1 or a fragment thereof.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may further comprise any of the mutated sequences described in US 20160369298, e.g., any of the following sequences SDSGASN (SEQ ID NO: 1550), SPSGASN (SEQ ID NO: 1551), SHSGASN (SEQ ID NO: 1552), SRSGASN (SEQ ID NO: 1553), SKSGASN (SEQ ID NO: 1554), SNSGASN (SEQ ID NO: 1555), SGSGASN (SEQ ID NO: 1556), SASGASN (SEQ ID NO: 1557), SESGTSN (SEQ ID NO: 1558), STTGGSN (SEQ ID NO: 1559), SSAGSTN (SEQ ID NO: 1560), NNDSQA (SEQ ID NO: 1561), NNRNQA (SEQ ID NO: 1562), NNNKQA (SEQ ID NO: 1563), NAKRQA (SEQ ID NO: 1564), NDEHQA (SEQ ID NO: 1565), NTSQKA (SEQ ID NO: 1566), YYLSRTNTPSGTDTQSRLVFSQAGA (SEQ ID NO: 1567), YYLSRTNTDSGTETQSGLDFSQAGA (SEQ ID NO: 1568), YYLSRTNTESGTPTQSALEFSQAGA (SEQ ID NO: 1569), YYLSRTNTHSGTHTQSPLHFSQAGA (SEQ ID NO: 1570), YYLSRTNTSSGTITISHLIFSQAGA (SEQ ID NO: 1571), YYLSRTNTRSGIMTKSSLMFSQAGA (SEQ ID NO: 1572), YYLSRTNTKSGRKTLSNLSFSQAGA (SEQ ID NO: 1573), YYLSRTNDGSGPVTPSKLRFSQRGA (SEQ ID NO: 1574), YYLSRTNAASGHATHSDLKFSQPGA (SEQ ID NO: 1575), YYLSRTNGQAGSLTMSELGFSQVGA (SEQ ID NO: 1576), YYLSRTNSTGGNQTTSQLLFSQLSA (SEQ ID NO: 1577), YFLSRTNNNTGLNTNSTLNFSQGRA (SEQ ID NO: 1578), SKTGADNNNSEYSWTG (SEQ ID NO: 1579), SKTDADNNNSEYSWTG (SEQ ID NO: 1580), SKTEADNNNSEYSWTG (SEQ ID NO: 1581), SKTPADNNNSEYSWTG (SEQ ID NO: 1582), SKTHADNNNSEYSWTG (SEQ ID NO: 1583), SKTQADNNNSEYSWTG SEQ ID NO: 1584), SKTIADNNNSEYSWTG (SEQ ID NO: 1585), SKTMADNNNSEYSWTG (SEQ ID NO: 1586), SKTRADNNNSEYSWTG (SEQ ID NO: 1587), SKTNADNNNSEYSWTG (SEQ ID NO: 1588), SKTVGRNNNSEYSWTG (SEQ ID NO: 1589), SKTADRNNNSEYSWTG (SEQ ID NO: 1590), SKKLSQNNNSKYSWQG (SEQ ID NO: 1591), SKPTTGNNNSDYSWPG (SEQ ID NO: 1592), STQKNENNNSNYSWPG (SEQ ID NO: 1593), HKDDEGKF (SEQ ID NO: 1594), HKDDNRKF (SEQ ID NO: 1595), HKDDTNKF (SEQ ID NO: 1596), HEDSDKNF (SEQ ID NO: 1597), HRDGADSF (SEQ ID NO: 1598), HGDNKSRF (SEQ ID NO: 1599), KQGSEKTNVDFEEV (SEQ ID NO: 1600), KQGSEKTNVDSEEV (SEQ ID NO: 1601), KQGSEKTNVDVEEV (SEQ ID NO: 1602), KQGSDKTNVDDAGV (SEQ ID NO: 1603), KQGSSKTNVDPREV (SEQ ID NO: 1604), KQGSRKTNVDHKQV (SEQ ID NO: 1605), KQGSKGGNVDTNRV (SEQ ID NO: 1606), KQGSGEANVDNGDV (SEQ ID NO: 1607), KQDAAADNIDYDHV (SEQ ID NO: 1608), KQSGTRSNAAASSV (SEQ ID NO: 1609), KENTNTNDTELTNV (SEQ ID NO: 1610), QRGNNVAATADVNT (SEQ ID NO: 1611), QRGNNEAATADVNT (SEQ ID NO: 1612), QRGNNPAATADVNT (SEQ ID NO: 1613), QRGNNHAATADVNT (SEQ ID NO: 1614), QEENNIAATPGVNT (SEQ ID NO: 1615), QPPNNMAATHEVNT (SEQ ID NO: 1616), QHHNNSAATTIVNT (SEQ ID NO: 1617), QTTNNRAAFNMVET (SEQ ID NO: 1618), QKKNNNAASKKVAT (SEQ ID NO: 1619), QGGNNKAADDAVKT (SEQ ID NO: 1620), QAAKGGAADDAVKT (SEQ ID NO: 1621), QDDRAAAANESVDT (SEQ ID NO: 1622), QQQHDDAAYQRVHT (SEQ ID NO: 1623), QSSSSLAAVSTVQT (SEQ ID NO: 1624), QNNQTTAAIRNVTT (SEQ ID NO: 1625), NYNKKSDNVDFT (SEQ ID NO: 1626), NYNKKSENVDFT (SEQ ID NO: 1627), NYNKKSLNVDFT (SEQ ID NO: 1628), NYNKKSPNVDFT (SEQ ID NO: 1629), NYSKKSHCVDFT (SEQ ID NO: 1630), NYRKTIYVDFT (SEQ ID NO: 1631), NYKEKKDVHFT (SEQ ID NO: 1632), NYGHRAIVQFT (SEQ ID NO: 1633), NYANHQFVVCT (SEQ ID NO: 1634), NYDDDPTGVLLT (SEQ ID NO: 1635), NYDDPTGVLLT (SEQ ID NO: 1636), NFEQQNSVEWT (SEQ ID NO: 1637), SQSGASN (SEQ ID NO: 1638), NNGSQA (SEQ ID NO: 1639), YYLSRTNTPSGTTTWSRLQFSQAGA (SEQ ID NO: 1640), SKTSADNNNSEYSWTG (SEQ ID NO: 1641), HKDDEEKF (SEQ ID NO: 1642), KQGSEKTNVDIEEV (SEQ ID NO: 1643), QRGNNQAATADVNT (SEQ ID NO: 1644), NYNKKSVNVDFT (SEQ ID NO: 1645), SQSGASNYNTPSGTTTQSRLQFSTSADNNNSEYSWTGATKYH (SEQ ID NO: 1646), SASGASNFNSEGGSLTQSSLGFSTDGENNNSDFSWTGATKYH (SEQ ID NO: 1647), SQSGASNYNTPSGTTTQSRLQFSTDGENNNSDFSWTGATKYH (SEQ ID NO: 1648), SASGASNYNTPSGTTTQSRLQFSTSADNNNSEFSWPGATTYH (SEQ ID NO: 1649), SQSGASNFNSEGGSLTQSSLGFSTDGENNNSDFSWTGATKYH (SEQ ID NO: 1650), SASGASNYNTPSGSLTQSSLGFSTDGENNNSDFSWTGATKYH (SEQ ID NO: 1651), SQSGASNYNTPSGTTTQSRLQFSTSADNNNSDFSWTGATKYH (SEQ ID NO: 1652), SGAGASNFNSEGGSLTQSSLGFSTDGENNNSDFSWTGATKYH (SEQ ID NO: 1653), SGAGASN (SEQ ID NO: 1654), NSEGGSLTQSSLGFS (SEQ ID NO: 1655), TDGENNNSDFS (SEQ ID NO: 1656), SEFSWPGATT (SEQ ID NO: 1657), TSADNNNSDFSWT (SEQ ID NO: 1658), SQSGASNY (SEQ ID NO: 1659), NTPSGTTTQSRLQFS (SEQ ID NO: 1660), TSADNNNSEYSWTGATKYH (SEQ ID NO: 1661), SASGASNF (SEQ ID NO: 1662), TDGENNNSDFSWTGATKYH (SEQ ID NO: 1663), SASGASNY (SEQ ID NO: 1664), TSADNNNSEFSWPGATTYH (SEQ ID NO: 1665), NTPSGSLTQSSLGFS (SEQ ID NO: 1666), TSADNNNSDFSWTGATKYH (SEQ ID NO: 1667), SGAGASNF (SEQ ID NO: 1668), CTCCAGVVSVVSMRSRVCVNSGCAGCTDHCVVSRNSGTCVMSACACAA (SEQ ID NO: 1669), CTCCAGAGAGGCAACAGACAAGCAGCTACCGCAGATGTCAACACACAA (SEQ ID NO: 1670), SAAGASN (SEQ ID NO: 1671), YFLSRTNTESGSTTQSTLRFSQAG (SEQ ID NO: 1672), SKTSADNNNSDFS (SEQ ID NO: 1673), KQGSEKTDVDIDKV (SEQ ID NO: 1674), STAGASN (SEQ ID NO: 1675), YFLSRTNTTSGIETQSTLRFSQAG (SEQ ID NO: 1676), SKTDGENNNSDFS (SEQ ID NO: 1677), KQGAAADDVEIDGV (SEQ ID NO: 1678), SEAGASN (SEQ ID NO: 1679), YYLSRTNTPSGTTTQSRLQFSQAG (SEQ ID NO: 1680), SKTSADNNNSEYS SEQ ID NO: 1681), KQGSEKTNVDIEKV (SEQ ID NO: 1682), YFLSRTNDASGSDTKSTLLFSQAG (SEQ ID NO: 1683), STTPSENNNSEYS (SEQ ID NO: 1684), SAAGATN (SEQ ID NO: 1685), YFLSRTNGEAGSATLSELRFSQAG (SEQ ID NO: 1686), HGDDADRF (SEQ ID NO: 1687), KQGAEKSDVEVDRV (SEQ ID NO: 1688), KQDSGGDNIDIDQV (SEQ ID NO: 1689), SDAGASN (SEQ ID NO: 1690), YFLSRTNTEGGHDTQSTLRFSQAG (SEQ ID NO: 1691), KEDGGGSDVAIDEV (SEQ ID NO: 1692), SNAGASN (SEQ ID NO: 1693), and YFLSRTNGEAGSATLSELRFSQPG (SEQ ID NO: 1694); or a nucleotide sequence that may encode the amino acid mutated sites of any of the following SEQ ID NO: 1695, SEQ ID NO: 1696, SEQ ID NO: 1697, SEQ ID NO: 1698, SEQ ID NO: 1699, SEQ ID NO: 1700, SEQ ID NO: 1701, SEQ ID NO: 1702, SEQ ID NO: 1703, SEQ ID NO: 1704, SEQ ID NO: 1705, SEQ ID NO: 1706, SEQ ID NO: 1707, SEQ ID NO: 1708, SEQ ID NO: 1709, SEQ ID NO: 1710, SEQ ID NO: 1711, SEQ ID NO: 1712, SEQ ID NO: 1713, SEQ ID NO: 1714, SEQ ID NO: 1715, SEQ ID NO: 1716, and SEQ ID NO: 1717.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may further comprise an ocular cell targeting peptide, e.g., as described in International Patent Publication WO2016134375, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to SEQ ID NO: 9, and SEQ ID NO:10 of WO2016134375. Further, any of the ocular cell targeting peptides or amino acids described in WO2016134375, may be inserted into any parent AAV capsid sequence, such as, but not limited to, AAV2 (SEQ ID NO:8 of WO2016134375; herein SEQ ID NO: 1718), or AAV9 (SEQ ID NO: 11 of WO2016134375; herein SEQ ID NO: 1719). The ocular cell targeting peptide may be, but is not limited to, any of the following amino acid sequences, GSTPPPM (SEQ ID NO: 1720), or GETRAPL (SEQ ID NO: 1721).

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be modified as described in the United States Publication US 20170145405 the contents of which are herein incorporated by reference in their entirety. AAV serotypes may include, modified AAV2 (e.g., modifications at Y444F, Y500F, Y730F and/or S662V), modified AAV3 (e.g., modifications at Y705F, Y731F and/or T492V), and modified AAV6 (e.g., modifications at S663V and/or T492V).

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be modified as described in the International Publication WO2017083722 the contents of which are herein incorporated by reference in their entirety. AAV serotypes may include, AAV1 (Y705+731F+T492V), AAV2 (Y444+500+730F+T491V), AAV3 (Y705+731F), AAV5, AAV 5 (Y436+693+719F), AAV6 (VP3 variant Y705F/Y731F/T492V), AAV8 (Y733F), AAV9, AAV9 (VP3 variant Y731F), and AAV10 (Y733F).

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may comprise, as described in International Patent Publication WO2017015102, the contents of which are herein incorporated by reference in their entirety, an engineered epitope comprising the amino acids SPAKFA (SEQ ID NO: 24 of WO2017015102; herein SEQ ID NO: 1722) or NKDKLN (SEQ ID NO:2 of WO2017015102; herein SEQ ID NO: 1723). The epitope may be inserted in the region of amino acids 665 to 670 based on the numbering of the VP1 capsid of AAV8 (SEQ ID NO: 3 of WO2017015102) and/or residues 664 to 668 of AAV3B (SEQ ID NO: 3).

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may be, or may have a sequence as described in International Patent Publication WO2017058892, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAV variants with capsid proteins that may comprise a substitution at one or more (e.g., 2, 3, 4, 5, 6, or 7) of amino acid residues 262-268, 370-379, 451-459, 472-473, 493-500, 528-534, 547-552, 709-710, 716-722 of AAV1, in any combination, or the equivalent amino acid residues in AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAV11, AAV12, AAVrh8, AAVrh10, AAVrh32.33, bovine AAV or avian AAV. The amino acid substitution may be, but is not limited to, any of the amino acid sequences described in WO2017058892. In some embodiments, the AAV may comprise an amino acid substitution at residues 256L, 258K, 259Q, 261S, 263A, 264S, 265T, 266G, 272H, 385S, 386Q, S472R, V473D, N500E 547S, 709A, 710N, 716D, 717N, 718N, 720L, A456T, Q457T, N458Q, K459S, T492S, K493A, S586R, S587G, S588N, T589R and/or 722T of AAV1 (SEQ ID NO: 1 of WO2017058892) in any combination, 244N, 246Q, 248R, 249E, 2501, 251K, 252S, 253G, 254S, 255V, 256D, 263Y, 377E, 378N, 453L, 456R, 532Q, 533P, 535N, 536P, 537G, 538T, 539T, 540A, 541T, 542Y, 543L, 546N, 653V, 654P, 656S, 697Q, 698F, 704D, 705S, 706T, 707G, 708E, 709Y and/or 710R of AAV5 (SEQ ID NO:5 of WO2017058892) in any combination, 248R, 316V, 317Q, 318D, 319S, 443N, 530N, 531S, 532Q 533P, 534A, 535N, 540A, 541 T, 542Y, 543L, 545G, 546N, 697Q, 704D, 706T, 708E, 709Y and/or 710R of AAV5 (SEQ ID NO: 5 of WO2017058892) in any combination, 264S, 266G, 269N, 272H, and/or 457Q, of AAV6 (SEQ ID NO:6 of WO2017058892) in any combination, 457T, 459N, 496G, 499N, and/or 500N, of AAV8 (SEQ ID NO: 8 of WO2017058892) in any combination, 4511, 452N, 453G, 454S, 455G, 456Q, 457N and/or 458Q of AAV9 (SEQ ID NO: 9 of WO2017058892) in any combination.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid may include a sequence of amino acids at positions 155, 156 and 157 of VP1 or at positions 17, 18, 19 and 20 of VP2, as described in International Publication No. WO 2017066764, the contents of which are herein incorporated by reference in their entirety. The sequences of amino acid may be, but not limited to, N-S-S, S-X-S, S-S-Y, N-X-S, N-S-Y, S-X-Y and N-X-Y, where N, X and Y are, but not limited to, independently non-serine, or non-threonine amino acids, wherein the AAV may be, but not limited to AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAV11 and AAV12. In some embodiments, the AAV may include a deletion of at least one amino acid at positions 156, 157 or 158 of VP1 or at positions 19, 20 or 21 of VP2, wherein the AAV may be, but not limited to AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAV11 and AAV12.

In some embodiments, the AAV capsid polypeptide, e.g., AAV capsid variant, or the parent AAV capsid further comprise one or more components generated by Cre-recombination-based AAV targeted evolution (CREATE) as described by Deverman et al., (Nature Biotechnology 34(2):204-209 (2016)), the contents of which are herein incorporated by reference in their entirety. In some embodiments, AAV serotypes generated in this manner have improved CNS transduction and/or neuronal and astrocytic tropism, as compared to other AAV serotypes.

Promoters

In some embodiments, an AAV particle comprising a novel capsid of the present disclosure, which may hereinafter also be referred to as an AAV particle comprising an AAV capsid polypeptide, e.g., AAV capsid variant (e.g., a TRACER AAV particle), comprises at least one element to enhance target specificity and expression (See e.g., Powell et al. Viral Expression Cassette Elements to Enhance Transgene Target Specificity and Expression in Gene Therapy, 2015; the contents of which are herein incorporated by reference in its entirety). Non-limiting examples of an element to enhance the target specificity and expression include promoters, endogenous miRNAs, post-transcriptional regulatory elements (PREs), polyadenylation (PolyA) signal sequences and upstream enhancers (USEs), CMV enhancers and introns.

A person skilled in the art may recognize that expression of the polypeptides in a target cell may require a specific promoter, including but not limited to, a promoter that is species specific, inducible, tissue-specific, or cell cycle-specific (Parr et al., Nat. Med. 3:1145-9 (1997); the contents of which are herein incorporated by reference in their entirety).

In some embodiments, the promoter is deemed to be efficient when it drives expression of the polypeptide(s) encoded by AAV capsid mRNA described herein. In some embodiments, the promoter is deemed to be efficient when it drives expression of the polypeptide(s) encoded by viral genomes encapsulated within a capsid described herein.

In some embodiments, the promoter drives expression of the polypeptides (e.g., AAV capsid polypeptides) for a period of time in targeted tissues. Expression driven by a promoter may be for a period of 1 hour, 2, hours, 3 hours, 4 hours, 5 hours, 6 hours, 7 hours, 8 hours, 9 hours, 10 hours, 11 hours, 12 hours, 13 hours, 14 hours, 15 hours, 16 hours, 17 hours, 18 hours, 19 hours, 20 hours, 21 hours, 22 hours, 23 hours, 1 day, 2 days, 3 days, 4 days, 5 days, 6 days, 1 week, 8 days, 9 days, 10 days, 11 days, 12 days, 13 days, 2 weeks, 15 days, 16 days, 17 days, 18 days, 19 days, 20 days, 3 weeks, 22 days, 23 days, 24 days, 25 days, 26 days, 27 days, 28 days, 29 days, 30 days, 31 days, 1 month, 2 months, 3 months, 4 months, 5 months, 6 months, 7 months, 8 months, 9 months, 10 months, 11 months, 1 year, 13 months, 14 months, 15 months, 16 months, 17 months, 18 months, 19 months, 20 months, 21 months, 22 months, 23 months, 2 years, 3 years, 4 years, 5 years, 6 years, 7 years, 8 years, 9 years, 10 years or more than 10 years. Expression may be for 1-5 hours, 1-12 hours, 1-2 days, 1-5 days, 1-2 weeks, 1-3 weeks, 1-4 weeks, 1-2 months, 1-4 months, 1-6 months, 2-6 months, 3-6 months, 3-9 months, 4-8 months, 6-12 months, 1-2 years, 1-5 years, 2-5 years, 3-6 years, 3-8 years, 4-8 years, or 5-10 years.

In some embodiments, the promoter drives expression of the polypeptides (e.g., AAV capsid polypeptides) for at least 1 month, 2 months, 3 months, 4 months, 5 months, 6 months, 7 months, 8 months, 9 months, 10 months, 11 months, 1 year, 2 years, 3 years 4 years, 5 years, 6 years, 7 years, 8 years, 9 years, 10 years, 11 years, 12 years, 13 years, 14 years, 15 years, 16 years, 17 years, 18 years, 19 years, 20 years, 21 years, 22 years, 23 years, 24 years, 25 years, 26 years, 27 years, 28 years, 29 years, 30 years, 31 years, 32 years, 33 years, 34 years, 35 years, 36 years, 37 years, 38 years, 39 years, 40 years, 41 years, 42 years, 43 years, 44 years, 45 years, 46 years, 47 years, 48 years, 49 years, 50 years, 55 years, 60 years, 65 years, or more than 65 years.

Promoters may be naturally occurring or non-naturally occurring. Non-limiting examples of promoters include viral promoters, plant promoters and mammalian promoters. In some embodiments, the promoters may be human promoters. In some embodiments, the promoter may be truncated.

Promoters which drive or promote expression in most tissues include, but are not limited to, human elongation factor 1α-subunit (EF1α), cytomegalovirus (CMV) immediate-early enhancer and/or promoter, chicken β-actin (CBA; such as, but not limited to, a CBA promotor as described in Miyazaki et al. (Gene. 1989 Jul. 15; 79(2):269-77, the contents of which are herein incorporated by reference in its entirety)) and its derivative CAG, β glucuronidase (GUSB), or ubiquitin C (UBC). Tissue-specific expression elements can be used to restrict expression to certain cell types such as, but not limited to, muscle specific promoters, B cell promoters, monocyte promoters, leukocyte promoters, macrophage promoters, pancreatic acinar cell promoters, endothelial cell promoters, lung tissue promoters, astrocyte promoters, or nervous system promoters which can be used to restrict expression to neurons, astrocytes, or oligodendrocytes.

Non-limiting examples of muscle-specific promoters include mammalian muscle creatine kinase (MCK) promoter, mammalian desmin (DES) promoter, mammalian troponin I (TNNI2) promoter, and mammalian skeletal alpha-actin (ASKA) promoter (see, e.g. U.S. Patent Publication US20110212529, the contents of which are herein incorporated by reference in their entirety). Muscle specific promotors may also include Mb promoter, myosin promotor, dystrophin promotor, dMCK and tMCK. As a non-limiting example, the muscle-specific promotor may be used to drive or promote expression in certain cell types, such as, but not limited to, myocytes and muscle stem cells.

Non-limiting examples of blood-specific promoters include B29 promoter, immunoglobulin heavy chain promoter, CD45 promoter, mouse INF-β promoter, CD45 SV40/CD45 promoter, WASP promoter, CD43 promoter, CD43 SV40/CD43 promoter, CD68 promoter, GPIIb promoter, CD14 promoter, and CD2 promoter. As a non-limiting example, the blood-specific promotor may be used to drive or promote expression in certain cell types, such as, but not limited to, in B cells, hematopoietic cells, leukocytes, platelets, macrophages, megakaryocytes, monocytes and/or T cells.

Non-limiting examples of bone-specific promotors include osteocalcin, bone sialoprotein, and OG-2 promoter. As a non-limiting example, the bone-specific promotor may be used to drive or promote expression in certain cell types, such as, but not limited to, osteoblasts and odontoblasts.

Non-limiting examples of eye-specific promotors include Chx10, PrP, Dkk3, Math5, Ptf1a, Pcp2, Nefh, gamma-synuclein gene (SNCG), Grik4, Pdgfra, Chat, Thy1.2, hVmd2, Thy1, Modified αA-crystallin, hRgp, mMo, Opn4, RLBP1, Glast, Foxg1, hVmd2, Trp1, Six3, cx36, Grm6-SV40 eukaryotic promoter, hVmd2, Dct, Rpc65, mRho, Irbp, hRho, Pcp2, Rhodopsin, and mSo, As a non-limiting example, the eye-specific promotor may be used to drive or promote expression in certain cell types, such as, but not limited, to retinal neurons, horizontal cells, bipolar cells, ganglion cells (GCs), ONL Müller cells, amacrine cells, lens cells, S-cone cells, M-cone cells, melanopsin-expressing GCs, neurons, ON bipolar, optic nerve cells, pigmented cells, retinal pigment epithelial cells, rod cells, rod bipolar cells, and rod photoreceptors.

Non-limiting examples of heart-specific promotors include MLC2v promoter, αMHC promoter, rat troponin T (Tnnt2), Tie2, and Tcf21. As a non-limiting example, the heart-specific promotor may be used to drive or promote expression in certain cell types, such as, but not limited to, cardiomyocytes, endothelial cells, and fibroblasts.

Non-limiting examples of kidney-specific promotors include, ECAD, NKCC2, KSPC, NPHS1, and SGLT2. As a non-limiting example, the kidney-specific promotor may be used to drive or promote expression in certain cell types, such as, but not limited to, collecting duct cells, loop of Henle cells, nephron cells, podocytes and proximal tubular cells.

Non-limiting examples of liver-specific promotors include, SV40/bA1b promoter, SV40/hA1b promoter, Hepatitis B virus core promoter, and Alpha fetoprotein. As a non-limiting example, the liver-specific promotor may be used to drive or promote expression in certain cell types, such as, but not limited to, hepatocytes.

Non-limiting examples of lung-specific promotors include Surfactant protein B promoter and Surfactant protein C promoter. As a non-limiting example, the lung-specific promotor may be used to drive or promote expression in certain cell types, such as, but not limited to, AT II cells and Clara cells.

Non-limiting examples of pancreas-specific promotors include elastase-1 promoter, PDX1 promoter, and insulin promoter. As a non-limiting example, the pancreas-specific promotor may be used to drive or promote expression in certain cell types, such as, but not limited to, acinar cells, beta cells, and Langerhans cells.

Non-limiting examples of vascular- or vasculature-specific promotors include Slco1c1, tie, cadherin, ICAM-2, claudin 1, Cldn5, Flt-1 promoter, and Endoglin promoter. As a non-limiting example, the vascular-specific promotor may be used to drive or promote expression in certain cell types, such as, endothelial cells. As a non-limiting example, the endothelial cell is a blood-brain barrier endothelial cell.

Non-limiting examples of tissue-specific expression elements for neurons include neuron-specific enolase (NSE), platelet-derived growth factor (PDGF), platelet-derived growth factor B-chain (PDGF-β), synapsin (Syn or Syn1), methyl-CpG binding protein 2 (MeCP2), Ca²⁺/calmodulin-dependent protein kinase II (CaMKII), metabotropic glutamate receptor 2 (mGluR2), neurofilament light (NFL) or heavy (NFH) chain, β-globin minigene nβ2, preproenkephalin (PPE), enkephalin (Enk), VGF, and excitatory amino acid transporter 2 (EAAT2) promoters. A non-limiting examples of tissue-specific expression elements for neuroectodermal stem cells is nestin.

Non-limiting examples of tissue-specific expression elements for astrocytes include glial fibrillary acidic protein (GFAP, GFabc1D) and EAAT2 promoters. A non-limiting example of a tissue-specific expression element for oligodendrocytes includes the myelin basic protein (MBP) promoter.

In some embodiments, the promoter may be less than 1 kb. The promoter may have a length of 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, or more than 800 nucleotides. The promoter may have a length between 200-300, 200-400, 200-500, 200-600, 200-700, 200-800, 300-400, 300-500, 300-600, 300-700, 300-800, 400-500, 400-600, 400-700, 400-800, 500-600, 500-700, 500-800, 600-700, 600-800, or 700-800.

In some embodiments, the promoter may be a combination of two or more components of the same or different starting or parental promoters such as, but not limited to, CMV and CBA. Each component may have a length of 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, or more than 800. Each component may have a length between 200-300, 200-400, 200-500, 200-600, 200-700, 200-800, 300-400, 300-500, 300-600, 300-700, 300-800, 400-500, 400-600, 400-700, 400-800, 500-600, 500-700, 500-800, 600-700, 600-800 or 700-800. In some embodiments, the promoter is a combination of a 382 nucleotide CMV-enhancer sequence and a 260 nucleotide CBA-promoter sequence.

In some embodiments, the AAV particle comprises a ubiquitous promoter. Non-limiting examples of ubiquitous promoters include CMV, CBA (including derivatives CAG, CB6, CBh, etc.), EF-1α, PGK, UBC, GUSB (hGBp), and UCOE (promoter of HNRPA2B1-CBX3).

Yu et al. (Molecular Pain 2011, 7:63; the contents of which are herein incorporated by reference in their entirety) evaluated the expression of eGFP under the CAG, EFIα, PGK and UBC promoters in rat DRG cells and primary DRG cells using lentiviral vectors and found that UBC showed weaker expression than the other 3 promoters and only 10-12% glial expression was seen for all promoters. Soderblom et al. (E. Neuro 2015; the contents of which are herein incorporated by reference in its entirety) evaluated the expression of eGFP in AAV8 with CMV and UBC promoters and AAV2 with the CMV promoter after injection in the motor cortex. Intranasal administration of a plasmid containing a UBC or EFIα promoter showed a sustained airway expression greater than the expression with the CMV promoter (See e.g., Gill et al., Gene Therapy 2001, Vol. 8, 1539-1546; the contents of which are herein incorporated by reference in their entirety). Husain et al. (Gene Therapy 2009; the contents of which are herein incorporated by reference in its entirety) evaluated an HβH construct with a hGUSB promoter, an HSV-1LAT promoter and an NSE promoter and found that the HβH construct showed weaker expression than NSE in mouse brain. Passini and Wolfe (J. Virol. 2001, 12382-12392, the contents of which are herein incorporated by reference in its entirety) evaluated the long-term effects of the HβH vector following an intraventricular injection in neonatal mice and found that there was sustained expression for at least 1 year. Low expression in all brain regions was found by Xu et al. (Gene Therapy 2001, 8, 1323-1332; the contents of which are herein incorporated by reference in their entirety) when NFL and NFH promoters were used as compared to the CMV-lacZ, CMV-luc, EF, GFAP, hENK, nAChR, PPE, PPE+wpre, NSE (0.3 kb), NSE (1.8 kb) and NSE (1.8 kb+wpre). Xu et al. found that the promoter activity in descending order was NSE (1.8 kb), EF, NSE (0.3 kb), GFAP, CMV, hENK, PPE, NFL and NFH. NFL is a 650 nucleotide promoter and NFH is a 920-nucleotide promoter which are both absent in the liver but NFH is abundant in the sensory proprioceptive neurons, brain and spinal cord and NFH is present in the heart. Scn8a is a 470 nucleotide promoter which expresses throughout the DRG, spinal cord and brain with particularly high expression seen in the hippocampal neurons and cerebellar Purkinje cells, cortex, thalamus, and hypothalamus (See e.g., Drews et al. Identification of evolutionary conserved, functional noncoding elements in the promoter region of the sodium channel gene SCN8A, Mamm Genome (2007) 18:723-731; and Raymond et al. Expression of Alternatively Spliced Sodium Channel α-subunit genes, Journal of Biological Chemistry (2004) 279(44) 46234-46241; the contents of each of which are herein incorporated by reference in their entireties).

Any of promoters taught by the aforementioned Yu, Soderblom, Gill, Husain, Passini, Xu, Drews, or Raymond may be used in the present disclosures.

In some embodiments, the promoter is ubiquitous. In some embodiments, the promoter is not cell specific.

In some embodiments, the promoter is a ubiquitin c (UBC) promoter. The UBC promoter may have a size of 300-350 nucleotides. As a non-limiting example, the UBC promoter is 332 nucleotides.

In some embodiments, the promoter is a β-glucuronidase (GUSB) promoter. The GUSB promoter may have a size of 350-400 nucleotides. As a non-limiting example, the GUSB promoter is 378 nucleotides.

In some embodiments, the promoter is a neurofilament light (NFL) promoter. The NFL promoter may have a size of 600-700 nucleotides. As a non-limiting example, the NFL promoter is 650 nucleotides.

In some embodiments, the promoter is a neurofilament heavy (NFH) promoter. The NFH promoter may have a size of 900-950 nucleotides. As a non-limiting example, the NFH promoter is 920 nucleotides.

In some embodiments, the promoter is a scn8a promoter. The scn8a promoter may have a size of 450-500 nucleotides. As a non-limiting example, the scn8a promoter is 470 nucleotides.

In some embodiments, the promoter is a phosphoglycerate kinase 1 (PGK) promoter.

In some embodiments, the promoter is a chicken β-actin (CBA) promoter, or a variant thereof.

In some embodiments, the promoter is a CB6 promoter.

In some embodiments, the promoter is a minimal CB promoter.

In some embodiments, the promoter is a P40 promoter. In some embodiments, the P40 promoter is located in the 3′ of the AAV capsid REP gene.

In some embodiments, the promoter is a cytomegalovirus (CMV) promoter.

In some embodiments, the CMV promoter is a hybrid CMV enhancer/Chicken beta-actin promoter sequence such as described by Niwa et al., 1991, the contents of which are incorporated herein by reference in their entirety.

In some embodiments, the promoter is a CAG promoter.

In some embodiments, the promoter is a GFAP promoter.

In some embodiments, the promoter is a synapsin (syn or syn1) promoter.

In some embodiments, the promoter is a liver or a skeletal muscle promoter. Non-limiting examples of liver promoters include human α-1-antitrypsin (hAAT) and thyroxine binding globulin (TBG). Non-limiting examples of skeletal muscle promoters include Desmin, MCK or synthetic C5-12.

In some embodiments, the promoter is an RNA pol III promoter. As a non-limiting example, the RNA pol III promoter is U6. As a non-limiting example, the RNA pol III promoter is H1.

In some embodiments, the promoter may be selected depending on the desired tropism. Non-limiting examples of promoters are described in WO2020072683, the contents of which are herein incorporated by reference in their entirety.

In some embodiments, the promoter drives capsid mRNA expression in the absence of helper virus co-infection.

In some embodiments, the AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant described herein) comprises two promoters. As a non-limiting example, the promoters are an P40 promoter and a CMV promoter. As another non-limiting example, the promoters are an P40 promoter and a cell-type specific promoter (e.g. synapsin).

In some embodiments, the AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant described herein) comprises an engineered promoter.

In another embodiment, the AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., AAV capsid variant described herein) comprises a promoter from a naturally expressed protein.

In some embodiments, a portion of the AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., AAV capsid variant described herein) REP gene is deleted to accommodate the promoter insertion. The promoter may be inserted upstream or downstream of the AAV particle CAP gene.

In some embodiments, the AAV particle of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., AAV capsid variant described herein) comprises a cell type-specific promoter to drive capsid mRNA expression. As a non-limiting example, the promotor is cell-type specific. The cell-type specific promotor may be synapsin. The cell-type specific promotor may be glial fibrillary acidic protein (GFAP). The AAV particle may comprise a P40 promoter and a cell-type specific promotor.

In some embodiments, the AAV particle of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., AAV capsid variant described herein) comprises a structure as shown in FIG. 4 . In some embodiments, an alternate backbone may be used for generation of the capsid libraries. In the alternate backbone, the forward version is changed into reverse, which can avoid the expression of capsid proteins and possible immune response to these foreign capsid proteins during in vivo evolution. In some embodiments, one or more additional WPRE elements may also be added to the backbone to improve RNA yield during viral library RNA recovery. In some embodiments, the backbone for the TRACER approach is as shown in FIG. 5 .

AAV Selection

The present disclosure provides methods of AAV selection for tissue- and/or cell type-specific transduction, whereby AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein) with high tropism for a tissue(s) and/or cell type(s) are identified and selected for use. In some embodiments, the AAV selection comprises administration of the AAV particles to a cell and/or a subject by standard methods known in the art (e.g. intravenously). In some embodiments, the AAV selection may comprise extraction of polynucleotides, e.g., capsid RNA, encoded by AAV particles, from a specific tissue and/or cell type. In some embodiments, the tissue may be non-nervous system tissue such as, but not limited to, liver, spleen and heart. The cells type may be, e.g., hepatocytes, Islets of Langerhans cells, and cardiomyocytes. In some embodiments, the tissue may be nervous system tissue such as, but not limited to, brain tissue, spinal cord tissue, and dorsal root ganglion tissue. The cell type may be, e.g., neurons, astrocytes, or oligodendrocytes. In some embodiments, the extracted RNA is enriched, reverse transcribed, and/or amplified. In some embodiments, the extracted RNA allows for recovery of full-length capsid “amplicon(s)” from a specific tissue and/or cell type, using various production methods, e.g., reverse transcription polymerase chain reaction (RT-PCR). As used herein, amplicon may refer to any piece of RNA or DNA formed as the product of amplification events, e.g. PCR. In some embodiments, full-length capsid amplicons may be used as templates for next generation sequencing (NGS) library generation. Full-length capsid amplicons may be used for cloning into a DNA library for the generation of AAV TRACER particles for any number of additional rounds of AAV selection as described above. In some embodiments, the AAV selection may be performed iteratively, or repeated, any number of times, or rounds. The above-described selection of AAV particles may also be more generally referred to herein as biopanning.

In some embodiments, the AAV selection comprises administration of the AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., AAV capsid variant, described herein) to a cell by standard methods known in the art (e.g. infection). As a non-limiting example, the cell is a HEK293 cell. As another non-limiting example, the cell is a nervous system cell such as, but not limited to, a neuron and/or a glial cell. As yet another non-limiting example, the cell is a brain microvascular endothelial cells (BMVEC). The BMVEC may be a human BMVEC (hBMVEC). The BMVEC may be a non-human primate (NHP) BMVEC.

In some embodiments, the AAV selection comprises administration of the AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., AAV capsid variant, described herein) to a rodent by standard methods known in the art (e.g. intravenously). The rodent may be a transgenic rodent or a non-transgenic (e.g., wild type) rodent. As a non-limiting example, the rodent is a rat or a mouse. Non-limiting examples of rats include Sprague Dawley, Wistar Albino, and Long Evans rats. Non-limiting examples of mice include BALB/C, FVB and C57BL/6 mice.

In some embodiments, the AAV selection comprises administration of the AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein) to a non-human primate (NHP) by standard methods known in the art (e.g. intravenously). Non-limiting examples of NHPs include rhesus macaques (Macaca mulatta) and cynomolgus macaques (Macaca fascicularis).

In some embodiments, the AAV selection comprises administration of AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein) to a rodent, non-human primate, and/or human cells. In some embodiments, the AAV selection comprises administration of AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein) to a rodent, non-human primate, and/or human subjects.

In some embodiments, the AAV selection may be performed iteratively, or repeated, any number of times, or rounds, within a single cell- or subject-type, wherein the single cell- or subject-type may remain unchanged, or the same, across AAV selection rounds. Cell types may be, e.g., HEK293 cells, hBMVECs, and NHP BMVECs. Subject types may be, e.g., Sprague Dawley rats, Wistar Albino rats, Long-Evans rats, BALB/C mice, FVB mice, C57BL/6 mice, rhesus macaques, cynomolgus macaques, and humans. As a non-limiting example, AAV selection is performed across one, two and/or three or more AAV selection rounds in the hBMVEC cell. As a non-limiting example, AAV selection is performed across one, two and/or three or more rounds in a mouse such as, but not limited to, a BALB/C mouse. As a non-limiting example, AAV selection is performed across one, two and/or three or more rounds in an NHP such as, but not limited to, a cynomolgus macaque, as represented in FIG. 1A and FIG. 1B.

AAV selection may be performed iteratively, or repeated, any number of times, or rounds, within any number of cell- and/or subject-types, wherein the cell- and or subject-type may change, or differ, across AAV selection rounds. As a non-limiting example, the AAV selection is performed a first round in a rhesus macaque, and an additional, e.g., subsequent, one, two, and/or or three or more rounds in a Sprague-Dawley rat.

AAV selection may be performed iteratively, or repeated, any number of times, or rounds, within any number of cell- and/or subject-types, and may additionally comprise the combination and/or comparison of any AAV capsid serotype as disclosed herein, or variants or derivatives thereof, with the AAV particle pool, at any AAV selection round. As a non-limiting example, the AAV capsid serotype comprises AAVF7/HSC7 (SEQ ID NO: 8 and 27 of WO2016049230). As another non-limiting example, the AAV capsid serotype comprises AAVF15/HSC15 (SEQ ID NO: 16 and 33 of WO2016049230). As yet another non-limiting example, the AAV capsid serotype AAVF17/HSC17 (SEQ ID NO: 13 and 35 of WO2016049230). The AAV selection round may be the first, second, third, or fourth AAV selection round.

Orthogonal Evolution

Methods of AAV selection of the present disclosure may comprise orthogonal evolution. In some embodiments, orthogonal evolution is a method wherein AAV particles are administered for a first round of AAV selection as described herein across a set of any number of cell- and/or subject-types that may be from different species and/or strains, and wherein any number of additional, e.g., subsequent, AAV selection rounds are performed either across a set of any number of cell- and/or subject-types that may be from different species and/or strains, or across a set of any number of cell- and/or subject-types that may be from the same species and/or strains, as represented in FIG. 2 . Cell types may be, e.g., HEK293 cells, hBMVECs, and NHP BMVECs. Subject types may be, e.g., Sprague Dawley rats, Wistar Albino rats, Long-Evans rats, BALB/C mice, FVB mice, C57BL/6 mice, rhesus macaques, cynomolgus macaques, and humans.

Viral Genome of the AAV Particle

AAV particle as described herein, comprising a peptide, e.g., a targeting peptide and/or selected by the TRACER methods (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid polypeptide, e.g., AAV capsid variant, described herein), may be used for the delivery of a viral genome to a tissue, e.g., a target tissue (e.g., CNS, DRG, and/or muscle). In some embodiments, an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein can be used for delivery of a viral genome to a tissue or cell, e.g., CNS, DRG, or muscle cell or tissue. In some embodiments, an AAV particle of the present disclosure is a recombinant AAV particle. In some embodiments, an AAV particle of the present disclosure is an isolated AAV particle.

The viral genome may encode any payload, such as but not limited to a polypeptide (e.g., a therapeutic polypeptide), an antibody, an enzyme, an RNAi agent and/or components of a gene editing system. In one embodiment, the AAV particles described herein are used to deliver a payload to cells of the CNS, after intravenous delivery. In another embodiment, the AAV particles described herein are used to deliver a payload to cells of the DRG, after intravenous delivery. In some embodiments, the AAV particles described herein are used to deliver a payload to cells of a muscle, e.g., a heart muscle, after intravenous delivery.

A viral genome of an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant, as described herein, comprises a nucleic acid sequence with at least one payload region encoding a payload, and at least one ITR. A viral genome typically comprises two ITR sequences, one at each of the 5′ and 3′ ends. Further, a viral genome of the AAV particles described herein may comprise nucleic acid sequences for additional components, such as, but not limited to, a regulatory element (e.g., promoter), untranslated regions (UTR), a polyadenylation sequence (polyA), a filler or stuffer sequence, an intron, and/or a linker sequence for enhanced expression.

These viral genome components can be selected and/or engineered to further tailor the specificity and efficiency of expression of a given payload in a target tissue (e.g., CNS, muscle, or DRG).

Viral Genome Component: Inverted Terminal Repeats (ITRs)

In some embodiments, the AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein comprises a viral genome with at least one ITR and a payload region. In one embodiment, the viral genome has two ITRs. These two ITRs flank the payload region at the 5′ and 3′ ends. The ITRs function as origins of replication comprising recognition sites for replication. ITRs comprise sequence regions which can be complementary and symmetrically arranged. ITRs incorporated into viral genomes as described herein may be comprised of naturally occurring polynucleotide sequences or recombinantly derived polynucleotide sequences.

The ITRs may be derived from the same serotype as the capsid polypeptide, e.g., capsid variant, selected from any of the known serotypes, or a derivative thereof. The ITR may be of a different serotype than the capsid. In one embodiment, the AAV particle has more than one ITR. In a non-limiting example, the AAV particle has a viral genome comprising two ITRs. In one embodiment, the ITRs are of the same serotype as one another. In another embodiment, the ITRs are of different serotypes. Non-limiting examples include zero, one or both of the ITRs having the same serotype as the capsid. In one embodiment both ITRs of the viral genome of the AAV particle are AAV2 ITRs.

Independently, each ITR may be about 100 to about 150 nucleotides in length. An ITR may be about 100-105 nucleotides in length, 106-110 nucleotides in length, 111-115 nucleotides in length, 116-120 nucleotides in length, 121-125 nucleotides in length, 126-130 nucleotides in length, 131-135 nucleotides in length, 136-140 nucleotides in length, 141-145 nucleotides in length or 146-150 nucleotides in length. In one embodiment, the ITRs are 140-142 nucleotides in length. Non-limiting examples of ITR length are 102, 105, 130, 140, 141, 142, 145 nucleotides in length. ITRs encompassed by the present disclosure include those with at least 90% identity, at least 95% identity, at least 98% identity, or at least 99% identity to a known AAV serotype ITR sequence.

Viral Genome Component: Promoters

In one embodiment, the payload region of the viral genome comprises at least one element to enhance the payload target specificity and expression (See e.g., Powell et al. Viral Expression Cassette Elements to Enhance Transgene Target Specificity and Expression in Gene Therapy, 2015; the contents of which are herein incorporated by reference in their entirety). Non-limiting examples of elements to enhance payload target specificity and expression include promoters, endogenous miRNAs, post-transcriptional regulatory elements (PREs), polyadenylation (PolyA) signal sequences and upstream enhancers (USEs), CMV enhancers and introns.

A person skilled in the art may recognize that expression of a payload in a target cell may require a specific promoter, including but not limited to, a promoter that is species specific, inducible, tissue-specific, or cell cycle-specific (Parr et al., Nat. Med. 3:1145-9 (1997); the contents of which are herein incorporated by reference in their entirety).

In one embodiment, the promoter is deemed to be efficient when it drives expression of the payload encoded by the viral genome of the AAV particle.

In one embodiment, the promoter is a promoter deemed to be efficient when it drives expression in a cell being targeted.

In one embodiment, the promoter is a promoter having a tropism for a cell being targeted.

In one embodiment, the promoter drives expression of the payload for a period of time in targeted tissues. Expression driven by a promoter may be for a period of 1 hour, 2, hours, 3 hours, 4 hours, 5 hours, 6 hours, 7 hours, 8 hours, 9 hours, 10 hours, 11 hours, 12 hours, 13 hours, 14 hours, 15 hours, 16 hours, 17 hours, 18 hours, 19 hours, 20 hours, 21 hours, 22 hours, 23 hours, 1 day, 2 days, 3 days, 4 days, 5 days, 6 days, 1 week, 8 days, 9 days, 10 days, 11 days, 12 days, 13 days, 2 weeks, 15 days, 16 days, 17 days, 18 days, 19 days, 20 days, 3 weeks, 22 days, 23 days, 24 days, 25 days, 26 days, 27 days, 28 days, 29 days, 30 days, 31 days, 1 month, 2 months, 3 months, 4 months, 5 months, 6 months, 7 months, 8 months, 9 months, 10 months, 11 months, 1 year, 13 months, 14 months, 15 months, 16 months, 17 months, 18 months, 19 months, 20 months, 21 months, 22 months, 23 months, 2 years, 3 years, 4 years, 5 years, 6 years, 7 years, 8 years, 9 years, 10 years or more than 10 years. Expression may be for 1-5 hours, 1-12 hours, 1-2 days, 1-5 days, 1-2 weeks, 1-3 weeks, 1-4 weeks, 1-2 months, 1-4 months, 1-6 months, 2-6 months, 3-6 months, 3-9 months, 4-8 months, 6-12 months, 1-2 years, 1-5 years, 2-5 years, 3-6 years, 3-8 years, 4-8 years or 5-10 years. As a non-limiting example, the promoter is a selected for sustained expression of a payload in tissues and/or cells of the central or peripheral nervous system.

Promoters may be naturally occurring or non-naturally occurring. Non-limiting examples of promoters include those derived from viruses, plants, mammals, or humans. In some embodiments, the promoters may be those derived from human cells or systems. In some embodiments, the promoter may be truncated or mutated.

Promoters which drive or promote expression in most tissues include, but are not limited to, the human elongation factor 1α-subunit (EF1a) promoter, the cytomegalovirus (CMV) immediate-early enhancer and/or promoter, the chicken β-actin (CBA) promoter and its derivative CAG, glucuronidase (GUSB) promoter, or ubiquitin C (UBC) promoter. Tissue-specific promoters can be used to restrict expression to certain cell types such as, but not limited to, cells of the central or peripheral nervous systems, targeted regions within (e.g., frontal cortex), and/or sub-sets of cells therein (e.g., excitatory neurons). As non-limiting examples, cell-type specific promoters may be used to restrict expression of a payload to excitatory neurons (e.g., glutamatergic), inhibitory neurons (e.g., GABA-ergic), neurons of the sympathetic or parasympathetic nervous system, sensory neurons, neurons of the dorsal root ganglia, motor neurons, or supportive cells of the nervous systems such as microglia, astrocytes, oligodendrocytes, and/or Schwann cells.

Cell-type specific promoters also exist for other tissues of the body, with non-limiting examples including, liver promoters (e.g., hAAT, TBG), skeletal muscle specific promoters (e.g., desmin, MCK, C512), B cell promoters, monocyte promoters, leukocyte promoters, macrophage promoters, pancreatic acinar cell promoters, endothelial cell promoters, lung tissue promoters, and/or cardiac or cardiovascular promoters (e.g., αMHC, cTnT, and CMV-MLC2k).

Non-limiting examples of tissue-specific promoters for targeting payload expression to central nervous system tissues and cells include synapsin (Syn), glutamate vesicular transporter (VGLUT), vesicular GABA transporter (VGAT), parvalbumin (PV), sodium channel Na_(v) 1.8, tyrosine hydroxylase (TH), choline acetyltransferase (ChaT), methyl-CpG binding protein 2 (MeCP2), Ca²⁺/calmodulin-dependent protein kinase II (CaMKII), metabotropic glutamate receptor 2 (mGluR2), neurofilament light (NFL) or heavy (NFH), neuron-specific enolase (NSE), β-globin minigene nβ2, preproenkephalin (PPE), enkephalin (Enk) and excitatory amino acid transporter 2 (EAAT2) promoters. Non-limiting examples of tissue-specific expression elements for astrocytes include glial fibrillary acidic protein (GFAP) and EAAT2 promoters. A non-limiting example of a tissue-specific expression element for oligodendrocytes includes the myelin basic protein (MBP) promoter.

In some embodiments, the promoter of the viral genome used to drive expression of the payload in a target tissue may be any of those noted herein for use in AAV particles.

Viral Genome Component: Untranslated Regions (UTRs)

In some embodiments, wild type untranslated regions (UTRs) of a gene are transcribed but not translated. Generally, the 5′ UTR starts at the transcription start site and ends at the start codon and the 3′ UTR starts immediately following the stop codon and continues until the termination signal for transcription.

Features typically found in abundantly expressed genes of specific target organs (e.g., CNS tissue, muscle, or DRG) may be engineered into UTRs to enhance stability and protein production. As a non-limiting example, a 5′ UTR from mRNA normally expressed in the brain (e.g., huntingtin) may be used in the viral genomes of the AAV particles described herein to enhance expression in neuronal cells or other cells of the central nervous system.

While not wishing to be bound by theory, wild-type 5′ untranslated regions (UTRs) include features which play roles in translation initiation. Kozak sequences, which are commonly known to be involved in the process by which the ribosome initiates translation of many genes, are usually included in 5′ UTRs. Kozak sequences have the consensus CCR(A/G)CCAUGG, where R is a purine (adenine or guanine) three bases upstream of the start codon (ATG), which is followed by another ‘G’.

In one embodiment, the 5′UTR in the viral genome includes a Kozak sequence.

In one embodiment, the 5′UTR in the viral genome does not include a Kozak sequence.

While not wishing to be bound by theory, wild-type 3′ UTRs are known to have stretches of Adenosines and Uridines embedded therein. These AU rich signatures are particularly prevalent in genes with high rates of turnover. Based on their sequence features and functional properties, the AU rich elements (AREs) can be separated into three classes (Chen et al, 1995, the contents of which are herein incorporated by reference in its entirety): Class I AREs, such as, but not limited to, c-Myc and MyoD, contain several dispersed copies of an AUUUA motif within U-rich regions. Class II AREs, such as, but not limited to, GM-CSF and TNF-α, possess two or more overlapping UUAUUUA(U/A)(U/A) nonamers. Class III ARES, such as, but not limited to, c-Jun and Myogenin, are less well defined. These U rich regions do not contain an AUUUA motif. Most proteins binding to the AREs are known to destabilize the messenger, whereas members of the ELAV family, most notably HuR, have been documented to increase the stability of mRNA. HuR binds to AREs of all the three classes. Engineering the HuR specific binding sites into the 3′ UTR of nucleic acid molecules will lead to HuR binding and thus, stabilization of the message in vivo.

Introduction, removal or modification of 3′ UTR AU rich elements (AREs) can be used to modulate the stability of a polynucleotide. When engineering specific polynucleotides, e.g., payload regions of viral genomes, one or more copies of an ARE can be introduced to make polynucleotides less stable and thereby curtail translation and decrease production of the resultant protein. Likewise, AREs can be identified and removed or mutated to increase the intracellular stability and thus increase translation and production of the resultant protein.

In one embodiment, the 3′ UTR of the viral genome may include an oligo(dT) sequence for templated addition of a poly-A tail.

In one embodiment, the viral genome may include at least one miRNA seed, binding site or full sequence. microRNAs (or miRNA or miR) are 19-25 nucleotide noncoding RNAs that bind to the sites of nucleic acid targets and down-regulate gene expression either by reducing nucleic acid molecule stability or by inhibiting translation. In some embodiments, a microRNA sequence comprises a seed region, e.g., a sequence in the region of positions 2-8 of the mature microRNA, which has Watson-Crick sequence fully or partially complementarity to the miRNA target sequence of the nucleic acid.

In one embodiment, the viral genome may be engineered to include, alter or remove at least one miRNA binding site, full sequence or seed region.

Any UTR from any gene known in the art may be incorporated into the viral genome of the AAV particle. These UTRs, or portions thereof, may be placed in the same orientation as in the gene from which they were selected or they may be altered in orientation or location. In one embodiment, the UTR used in the viral genome of the AAV particle may be inverted, shortened, lengthened, made with one or more other 5′ UTRs or 3′ UTRs known in the art. As used herein, the term “altered” as it relates to a UTR, means that the UTR has been changed in some way in relation to a reference sequence. For example, a 3′ or 5′ UTR may be altered relative to a wild type or native UTR by the change in orientation or location as taught above or may be altered by the inclusion of additional nucleotides, deletion of nucleotides, swapping or transposition of nucleotides.

In one embodiment, the viral genome of the AAV particle comprises at least one artificial UTR which is not a variant of a wild type UTR.

In one embodiment, the viral genome of the AAV particle comprises UTRs which have been selected from a family of transcripts whose proteins share a common function, structure, feature or property.

Viral Genome Component: Polyadenylation Sequence

The viral genome of the AAV particle described herein (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein) may comprise at least one polyadenylation sequence. In one embodiment, the viral genome of the AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein) comprises a polyadenylation sequence between the 3′ end of the payload encoding region and the 5′ end of the 3′ITR.

In one embodiment, the polyadenylation sequence or “polyA sequence” may range from absent to about 500 nucleotides in length. In some embodiments, the polyA sequence comprises a length of about 100-600 nucleotides, e.g., about 100-500 nucleotides, about 100-400 nucleotides, about 100-300 nucleotides, about 100-200 nucleotides, about 200-600 nucleotides, about 200-500 nucleotides, about 200-400 nucleotides, about 200-300 nucleotides, about 300-600 nucleotides, about 300-500 nucleotides, about 300-400 nucleotides, about 400-600 nucleotides, about 400-500 nucleotides, or about 500-600 nucleotides. In some embodiments, the polyA signal region comprises a length of about 100 to 150 nucleotides, e.g., about 127 nucleotides. In some embodiments, the polyA sequence comprises a length of about 450 to 500 nucleotides, e.g., about 477 nucleotides. In some embodiments, the polyA sequence comprises a length of about 520 to about 560 nucleotides, e.g., about 552 nucleotides. In some embodiments, the polyA sequence comprises a length of about 127 nucleotides.

Viral Genome Component: Introns

In one embodiment, the viral genome of the AAV particle as described herein (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant), comprises at least one element to enhance the payload target specificity and expression (See e.g., Powell et al. Viral Expression Cassette Elements to Enhance Transgene Target Specificity and Expression in Gene Therapy, Discov. Med, 2015, 19(102): 49-57; the contents of which are herein incorporated by reference in their entirety) such as an intron. Non-limiting examples of introns include, MVM (67-97 bps), F.IX truncated intron 1 (300 bps), β-globin SD/immunoglobulin heavy chain splice acceptor (250 bps), adenovirus splice donor/immunoglobin splice acceptor (500 bps), SV40 late splice donor/splice acceptor (19S/16S) (180 bps) and hybrid adenovirus splice donor/IgG splice acceptor (230 bps).

In one embodiment, the intron or intron portion may be 100-500 nucleotides in length. The intron may have a length of 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490 or 500 nucleotides. The intron may have a length between 80-100, 80-120, 80-140, 80-160, 80-180, 80-200, 80-250, 80-300, 80-350, 80-400, 80-450, 80-500, 200-300, 200-400, 200-500, 300-400, 300-500, or 400-500 nucleotides.

Viral Genome Component: Stuffer Sequences

In one embodiment, the viral genome of the AAV particle described herein (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant), comprises at least one element to improve packaging efficiency and expression, such as a stuffer or filler sequence. Non-limiting examples of stuffer sequences include albumin and/or alpha-1 antitrypsin. Any known viral, mammalian, or plant sequence may be manipulated for use as a stuffer sequence.

In one embodiment, the stuffer or filler sequence may be from about 100-3500 nucleotides in length. The stuffer sequence may have a length of about 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100, 1200, 1300, 1400, 1500, 1600, 1700, 1800, 1900, 2000, 2100, 2200, 2300, 2400, 2500, 2600, 2700, 2800, 2900 or 3000 nucleotides.

Viral Genome Component: miRNA

In one embodiment, the viral genome comprises at least one sequence encoding a miRNA to reduce the expression of the payload in an “off-target” tissue. As used herein, “off-target” indicates a tissue or cell-type unintentionally targeted by the AAV particles described herein. miRNAs and their targeted tissues are well known in the art. As an example, an “off-target” tissue or cell when targeting the DRG (dorsal root ganglion), may be neurons of other ganglia, such as those of the sympathetic or parasympathetic nervous system. In some embodiments, a miRNA, e.g., a miR183, a miR182, and/or miR96, may be encoded in the viral genome to modulate, e.g., reduce the expression, of the viral genome in a DRG neuron. As another non-limiting example, a miR-122 miRNA may be encoded in the viral genome to modulate, e.g., reduce, the expression of the viral genome in the liver. In some embodiments, a miRNA, e.g., a miR-142-3p, may be encoded in the viral genome to modulate, e.g., reduce, the expression, of the viral genome in a cell or tissue of the hematopoietic lineage, including for example immune cells (e.g., antigen presenting cells or APC, including dendritic cells (DCs), macrophages, and B-lymphocytes).

Viral Genome Component: miR Binding Site

Tissue- or cell-specific expression of the AAV viral particles of the invention can be enhanced by introducing tissue- or cell-specific regulatory sequences, e.g., promoters, enhancers, microRNA binding sites, e.g., a detargeting site. Without wishing to be bound by theory, it is believed that a miR binding site can modulate, e.g., prevent, suppress, or otherwise inhibit, the expression of a gene of interest on the viral genome of the invention, based on the expression of the corresponding endogenous microRNA (miRNA) or a corresponding controlled exogenous miRNA in a tissue or cell, e.g., a non-targeting cell or tissue. In some embodiments, a miR binding site modulates, e.g., reduces, expression of the payload encoded by a viral genome of an AAV particle described herein in a cell or tissue where the corresponding mRNA is expressed.

In some embodiments, the viral genome of an AAV particle described herein comprises a microRNA binding site, e.g., a detargeting site. In some embodiments, the viral genome of an AAV particle described herein comprises a coding sequence for a miR binding site, a microRNA binding site series (miR BSs), or a reverse complement thereof.

In some embodiments, the miR binding site series or the miR binding site is located in the 3′-UTR region of the viral genome (e.g., 3′ relative to the nucleic acid sequence encoding a payload), e.g., before the polyA sequence, 5′-UTR region of the viral genome (e.g., 5′ relative to the nucleic acid sequence encoding a payload), or both.

In some embodiments, the miR binding site series comprise at least 1-5 copies, e.g., at least 1-3, 2-4, 3-5, 1, 2, 3, 4, 5 or more copies of a miR binding site (miR BS). In some embodiments, all copies are identical, e.g., comprise the same miR binding site. In some embodiments, the miR binding sites within the miR binding site series are continuous and not separated by a spacer. In some embodiments, the miR binding sites within a miR binding site series are separated by a spacer, e.g., a non-coding sequence. In some embodiments, the spacer is at least about 5 to 10 nucleotides, e.g., about 7-8 nucleotides, nucleotides in length. In some embodiments, the spacer comprises one or more of (i) GGAT; (ii) CACGTG; (iii) GCATGC, or a repeat of one or more of (i)-(iii).

In some embodiments, the miR binding site series comprise at least 1-5 copies, e.g., at least 1-3, 2-4, 3-5, 1, 2, 3, 4, 5 or more copies of a miR binding site (miR BS). In some embodiments, at least 1, 2, 3, 4, 5, or all of the copies are different, e.g., comprise a different miR binding site. In some embodiments, the miR binding sites within the miR binding site series are continuous and not separated by a spacer. In some embodiments, the miR binding sites within a miR binding site series are separated by a spacer, e.g., a non-coding sequence. In some embodiments, the spacer is at least about 5 to 10 nucleotides, e.g., about 7-8 nucleotides, in length. In some embodiments, the spacer comprises one or more of (i) GGAT; (ii) CACGTG; (iii) GCATGC, or a repeat of one or more of (i)-(iii).

In some embodiments, the miR binding site is substantially identical (e.g., at least 70%, 75%, 80%, 85%, 90%, 95%, 99% or 100% identical), to the miR in the host cell. In some embodiments, the miR binding site comprises at least 1, 2, 3, 4, or 5 mismatches or no more than 6, 7, 8, 9, or 10 mismatches to a miR in the host cell. In some embodiments, the mismatched nucleotides are contiguous. In some embodiments, the mismatched nucleotides are non-contiguous. In some embodiments, the mismatched nucleotides occur outside the seed region-binding sequence of the miR binding site, such as at one or both ends of the miR binding site. In some embodiments, the miR binding site is 100% identical to the miR in the host cell.

In some embodiments, a miR binding site or sequence region is at least about 10 to about 125 nucleotides in length, e.g., at least about 10 to 50 nucleotides, 10 to 100 nucleotides, 50 to 100 nucleotides, 50 to 125 nucleotides, or 100 to 125 nucleotides in length. In some embodiments, a miR binding site or sequence region is at least about 7 to about 28 nucleotides in length, e.g., at least about 8-28 nucleotides, 7-28 nucleotides, 8-18 nucleotides, 12-28 nucleotides, 20-26 nucleotides, 22 nucleotides, 24 nucleotides, or 26 nucleotides in length, and optionally comprises at least one consecutive region (e.g., 7 or 8 nucleotides) complementary to the seed sequence of a miRNA (e.g., a miR122, a miR142, a miR183).

In some embodiments, the miR binding site is complementary to a miR expressed in liver or hepatocytes, such as miR122. In some embodiments, the miR binding site or miR binding site series comprises a miR122 binding site sequence. In some embodiments, the miR122 binding site comprises the nucleotide sequence of ACAAACACCATTGTCACACTCCA (SEQ ID NO: 3672), or a nucleotide sequence having at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, at least 95%, at least 99%, or 100% sequence identity, or having at least one, two, three, four, five, six, or seven modifications but no more than ten modifications to SEQ ID NO: 3672, e.g., wherein the modification can result in a mismatch between the miR binding site and the corresponding miRNA. In some embodiments, the miR122 binding site comprises at least 3, 4, or 5 copies of a miR122 binding site, optionally comprising the nucleotide sequence of: ACAAACACCATTGTCACACTCCACACAAACACCATTGTCACACTCCACACAAACACCATTGTCACACT CCA (SEQ ID NO: 3673), or a sequence having at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, at least 95%, at least 99%, or 100% sequence identity, or having at least one, two, three, four, five, six, or seven modifications but no more than ten modifications to SEQ ID NO: 3673, e.g., wherein the modification can result in a mismatch between the miR binding site and the corresponding miRNA. In some embodiments, at least two of the miR122 binding sites are connected directly, e.g., without a spacer. In other embodiments, at least two of the miR122 binding sites are separated by a spacer, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides in length, which is located between two or more consecutive miR122 binding site sequences. In embodiments, the spacer is at least about 5 to 10 nucleotides, e.g., about 7-8, in length. In some embodiments, the spacer comprises one or more of (i) GGAT; (ii) CACGTG; (iii) GCATGC, or repeats thereof. In some embodiments, a miR binding site series comprises at least 3-5 copies, e.g., 4 copies, of a miR122 binding site, with or without a spacer, wherein the spacer is at least about 5 to 10 nucleotides, e.g., about 7-8 nucleotides, in length.

In some embodiments, the miR binding site is complementary to a miR expressed in hematopoietic lineage, including immune cells (e.g., antigen presenting cells or APC, including dendritic cells (DCs), macrophages, and B-lymphocytes). In some embodiments, the miR binding site complementary to a miR expressed in hematopoietic lineage comprises a nucleotide sequence disclosed, e.g., in US 2018/0066279, the contents of which are incorporated by reference herein in its entirety.

In some embodiments, the miR binding site or miR binding site series comprises a miR-142-3p binding site sequence. In some embodiments, the miR-142-3p binding site comprises the nucleotide sequence of TCCATAAAGTAGGAAACACTACA (SEQ ID NO: 3674), a sequence having at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, at least 95%, at least 99%, or 100% sequence identity, or having at least one, two, three, four, five, six, or seven modifications but no more than ten modifications to SEQ ID NO: 3674, e.g., wherein the modification can result in a mismatch between the miR binding site and the corresponding miRNA. In some embodiments, the miR-142-3p binding site comprises at least 3, 4, or 5 copies of a miR-142-3p binding site. In some embodiments, a miR binding site series comprises at least 3-5 copies, e.g., 4 copies, of a miR-142-3p binding site, with or without a spacer, wherein the spacer is at least about 5 to 10 nucleotides, e.g., about 7-8 nucleotides, in length.

In some embodiments, the miR binding site is complementary to a miR expressed in a DRG (dorsal root ganglion) neuron, e.g., a miR183, a miR182, and/or miR96 binding site. In some embodiments, the miR binding site complementary to a miR expressed in expressed in a DRG neuron comprises a nucleotide sequence disclosed, e.g., in WO2020/132455, the contents of which are incorporated by reference herein in its entirety.

In some embodiments, the miR binding site or miR binding site series comprises a miR183 binding site sequence. In some embodiments, the miR183 binding site comprises the nucleotide sequence of AGTGAATTCTACCAGTGCCATA (SEQ ID NO: 3675), or a sequence having at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, at least 95%, at least 99%, or 100% sequence identity, or having at least one, two, three, four, five, six, or seven modifications but no more than ten modifications to SEQ ID NO: 3675, e.g., wherein the modification can result in a mismatch between the miR binding site and the corresponding miRNA. In some embodiments, the sequence complementary to the seed sequence corresponds to the double underlined of the miR-183 binding site sequence. In some embodiments, the miR183 binding site comprises at least 3, 4, or 5 copies of a miR183 binding site. In some embodiments, a miR binding site series comprises at least 3-5 copies, e.g., 4 copies, of a miR183 binding site, with or without a spacer, wherein the spacer is at least about 5 to 10 nucleotides, e.g., about 7-8 nucleotides, in length.

In some embodiments, the miR binding site or miR binding site series comprises a miR182 binding site sequence. In some embodiments, the miR182 binding site comprises, the nucleotide sequence of AGTGTGAGTTCTACCATTGCCAAA (SEQ ID NO: 3676), a sequence having at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, at least 95%, at least 99%, or 100% sequence identity, or having at least one, two, three, four, five, six, or seven modifications but no more than ten modifications to SEQ ID NO: 3676, e.g., wherein the modification can result in a mismatch between the miR binding site and the corresponding miRNA. In some embodiments, the miR182 binding site comprises at least 3, 4, or 5 copies of a miR182 binding site. In some embodiments, a miR binding site series comprises at least 3-5 copies, e.g., 4 copies, of a miR182 binding site, with or without a spacer, wherein the spacer is at least about 5 to 10 nucleotides, e.g., about 7-8 nucleotides, in length.

In certain embodiments, the miR binding site or miR binding site series comprises a miR96 binding site sequence. In some embodiments, the miR96 binding site comprises the nucleotide sequence of AGCAAAAATGTGCTAGTGCCAAA (SEQ ID NO: 3677), a sequence having at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, at least 95%, at least 99%, or 100% sequence identity, or having at least one, two, three, four, five, six, or seven modifications but no more than ten modifications to SEQ ID NO: 3677, e.g., wherein the modification can result in a mismatch between the miR binding site and the corresponding miRNA. In some embodiments, the miR96 binding site comprises at least 3, 4, or 5 copies of a miR96 binding site. In some embodiments, a miR binding site series comprises at least 3-5 copies, e.g., 4 copies, of a miR96 binding site, with or without a spacer, wherein the spacer is at least about 5 to 10 nucleotides, e.g., about 7-8, in length.

In some embodiments, the miR binding site series comprises a miR122 binding site, a miR142 binding site, a miR183 binding site, a miR182 binding site, a miR 96 binding site, or a combination thereof. In some embodiments, the miR binding site series comprises at least 3, 4, or 5 copies of a miR122 binding site, a miR142 binding site, a miR183 binding site, a miR182 binding site, a miR 96 binding site, or a combination thereof. In some embodiments, at least two of the miR binding sites are connected directly, e.g., without a spacer. In other embodiments, at least two of the miR binding sites are separated by a spacer, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides in length, which is located between two or more consecutive miR binding site sequences. In embodiments, the spacer is at least about 5 to 10 nucleotides, e.g., about 7-8 nucleotides, in length. In some embodiments, the spacer comprises one or more of (i) GGAT; (ii) CACGTG; (iii) GCATGC, or a repeat of one or more of (i)-(iii). In some embodiments, a miR binding site series comprises at least 3-5 copies, e.g., 4 copies, of a combination of at least two, three, four, five, or all of a miR122 binding site, a miR142 binding site, a miR183 binding site, a miR182 binding site, a miR96 binding site, with or without a spacer, wherein the spacer is at least about 5 to 10 nucleotides, e.g., about 7-8 nucleotides, in length.

Viral Genome Component: Selectable Marker

In some embodiments, the viral genome of the AAV particle described herein (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant), optionally encodes a selectable marker. The selectable marker may comprise a cell-surface marker, such as any protein expressed on the surface of the cell including, but not limited to receptors, CD markers, lectins, integrins, or truncated versions thereof.

In some embodiments, selectable marker reporter genes are described in International Publication Nos. WO 1996023810 and WO 1996030540; Heim et al., Current Biology 2:178-182 (1996); Heim et al., Proc. Natl. Acad. Sci. USA (1995); or Heim et al., Science 373:663-664 (1995), the contents of each of which are incorporated herein by reference in their entirety.

Genome Size

In one embodiment, the AAV particle described herein (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant), may comprise a single-stranded or double-stranded viral genome. The size of the viral genome may be small, medium, large or the maximum size. As described above, the viral genome may comprise a promoter and a polyA tail.

In one embodiment, the viral genome may be a small single stranded viral genome. A small single stranded viral genome may be 2.1 to 3.5 kb in size such as, but not limited to, about 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.0, 3.1, 3.2, 3.3, 3.4, and 3.5 kb in size.

In one embodiment, the viral genome may be a small double stranded viral genome. A small double stranded viral genome may be 1.3 to 1.7 kb in size such as, but not limited to, about 1.3, 1.4, 1.5, 1.6, and 1.7 kb in size.

In one embodiment, the viral genome may be a medium single stranded viral genome. A medium single stranded viral genome may be 3.6 to 4.3 kb in size such as, but not limited to, about 3.6, 3.7, 3.8, 3.9, 4.0, 4.1, 4.2 and 4.3 kb in size.

In one embodiment, the viral genome may be a medium double stranded viral genome. A medium double stranded viral genome may be 1.8 to 2.1 kb in size such as, but not limited to, about 1.8, 1.9, 2.0, and 2.1 kb in size.

In one embodiment, the viral genome may be a large single stranded viral genome. A large single stranded viral genome may be 4.4 to 6.0 kb in size such as, but not limited to, about 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9 and 6.0 kb in size.

In one embodiment, the viral genome may be a large double stranded viral genome. A large double stranded viral genome may be 2.2 to 3.0 kb in size such as, but not limited to, about 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9 and 3.0 kb in size.

Payloads

The AAV particles of the present disclosure (e.g. an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) comprise a viral genome with at least one payload region. In some embodiments, a payload region may be a nucleic acid sequence within the viral genome of an AAV particle described herein, which encodes a payload, wherein the payload is an RNAi agent or a polypeptide. Payloads of the present disclosure may be, but are not limited to, peptides, polypeptides, proteins, antibodies, RNAi agents, etc.

In some embodiments, the payload region may comprise a combination of coding and non-coding nucleic acid sequences. In some embodiments, the payload region may encode a coding or non-coding RNA.

In one embodiment, the AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein) comprises a viral genome with a payload region encoding more than one payload of interest. In such an embodiment, a viral genome encoding more than one payload may be replicated and packaged into a viral particle. A target cell transduced with a viral particle comprising more than one payload may express each of the payloads in a single cell.

In some embodiments, the AAV particles described herein, e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant, comprises a nucleic acid encoding a payload. In some embodiments, the encoded payload comprises a therapeutic protein, an antibody, an enzyme, one or more components of a genome editing system, and/or an RNAi agent (e.g., a dsRNA, siRNA, shRNA, pre-miRNA, pri-miRNA, miRNA, stRNA, lncRNA, piRNA, or snoRNA). In some embodiments, the encoded payload modulates, e.g., increases or decreases, the presence, level, and/or activity of a gene, mRNA, protein, or a combination thereof, e.g., in a cell or a tissue.

Polypeptides

In some embodiments, the payload of AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein comprises a polypeptide, e.g., a polypeptide described herein. Where the payload region encodes a polypeptide, the polypeptide may be a peptide or protein. The payload region may encode a product of any known gene and/or a recombinant version thereof. As a non-limiting example, the payload region may encode at least one allele of apolipoprotein E (APOE) such as, but not limited to ApoE2, ApoE3 and/or ApoE4. In one embodiment, the payload region encodes ApoE2 (cys112, cys158). In one embodiment, the payload region encodes ApoE3 (cys112, arg158). In one embodiment, the payload region of encodes ApoE4 (arg112, arg158). As another non-limiting example, the payload region may encode aromatic L-amin acid decarboxylase (AADC). As another non-limiting example, the payload region may encode an antibody, or a fragment thereof. As another non-limiting example, the payload region may encode human survival of motor neuron (SMN) 1 or SMN2, or fragments or variants thereof. As another non-limiting example, the payload region may encode glucocerebrosidase (GBA1), or a fragment or variant thereof. As another non-limiting example, the payload region may encode granulin precursor or progranulin (GRN), or a fragment or variant thereof. As another non-limiting example, the payload region may encode aspartoacylase (ASPA), or a fragment or variant thereof. As another non-limiting example, the payload region may encode tripeptidyl peptidase I (CLN2), or a fragment or variant thereof. As another non-limiting example, the payload region may encode beta-galactosidase (GLB1), or a fragment or variant thereof. As another non-limiting example, the payload region may encode N-sulphoglucosamine sulphohydrolase (SGSH), or a fragment or variant thereof. As another non-limiting example, the payload region may encode N-acetyl-alpha-glucosaminidase (NAGLU), or a fragment or variant thereof. As another non-limiting example, the payload region may encode iduronate 2-sulfatase (IDS), or a fragment or variant thereof. As another non-limiting example, the payload region may encode Intracellular cholesterol transporter (NPC1), or a fragment or variant thereof. As another non-limiting example, the payload region may encode gigaxonin (GAN), or a fragment or variant thereof. The AAV viral genomes encoding polypeptides described herein may be useful in the fields of human disease, viruses, infections veterinary applications and a variety of in vivo and in vitro settings.

Amino acid sequences encoded by payload regions of the viral genomes described herein, may be translated as a whole polypeptide, a plurality of polypeptides or fragments of polypeptides, which independently may be encoded by one or more nucleic acids, fragments of nucleic acids or variants of any of the aforementioned.

Antibodies and Antibody Binding Fragments

In some embodiments, the payload of AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein comprises an antibody or antibody binding fragment. Where the payload region encodes an antibody, the “antibody” may be a full antibody, a fragment, or any derivative thereof, which forms a “functional antibody”, exhibiting the desired biological activity. As non-limiting examples, an antibody may be a native antibody (e.g., with two heavy and two light chains), a heavy chain variable region, a light chain variable region, a heavy chain constant region, a light chain constant region, Fab, Fab′, F(ab′)₂, Fv, or scFv fragments, a diabody, a linear antibody, a single-chain antibody, a multi-specific antibody, an intrabody, one or more heavy chain complementarity determining regions (CDR), one or more light chain CDRs, a bispecific antibody, a monoclonal antibody, a polyclonal antibody, a humanized antibody, an antibody mimetic, an antibody variant, a miniaturized antibody, a unibody, a maxibody, and/or a chimeric antigen receptor.

A payload region of an AAV particle described herein (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) may encode a polypeptide that forms or functions as any antibody, including antibodies that are known in the art and/or antibodies that are commercially available. The encoded antibody or antibody binding fragment may be therapeutic, diagnostic, or for research purposes. The encoded antibody or antibody binding fragment may be useful in the treatment of a neurological disease, a neurodegenerative disorder, a muscular disease, a neuromuscular disorder, a neuro-oncological disorder, or any disorder associated with the central and/or peripheral nervous systems.

In some embodiments, the viral genome of the AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant described herein) may comprise a nucleic acid which has been engineered to enable or enhance the expression of an antibody, or antibody binding fragment thereof.

In some embodiments, the encoded antibody of the payload of an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein comprises at least one immunoglobulin variable domain sequence. An antibody may include, for example, full-length, mature antibodies and antigen-binding fragments of an antibody. For example, an antibody can include a heavy (H) chain variable domain sequence (VH), and a light (L) chain variable domain sequence (VL). In another example, an antibody includes two heavy (H) chain variable domain sequences and two light (L) chain variable domain sequence, thereby forming two antigen binding sites, such as Fab, Fab′, F(ab′)2, Fc, Fd, Fd′, Fv, single chain antibodies (scFv for example), single variable domain antibodies, diabodies (Dab) (bivalent and bispecific), and chimeric (e.g., humanized) antibodies, which may be produced by the modification of whole antibodies or those synthesized de novo using recombinant DNA technologies. These functional antibody fragments, e.g., an antibody binding fragments, retain the ability to selectively bind with their respective antigen or receptor.

In some embodiments, the antibody binding fragment comprises at least one portion of an intact antibody, or recombinant variants thereof, and refers to the antigen binding domain, for example, an antigenic determining variable region of an intact antibody, that is sufficient to confer recognition and specific binding of the antibody fragment to a target, such as an antigen. Examples of antigen binding fragments include: (i) a Fab fragment, a monovalent fragment consisting of the VL, VH, CL and CH1 domains; (ii) a F(ab′)2 fragment, a bivalent fragment comprising two Fab fragments linked by a disulfide bridge at the hinge region; (iii) a Fd fragment consisting of the VH and CH1 domains; (iv) a Fv fragment consisting of the VL and VH domains of a single arm of an antibody, (v) a diabody (dAb) fragment, which consists of a VH domain; (vi) a camelid or camelized variable domain; (vii) a single chain Fv (scFv), see e.g., Bird et al. (1988) Science 242:423-426; and Huston et al. (1988) Proc. Natl. Acad. Sci. USA 85:5879-5883); and (viii) a single domain antibody. These antibody fragments are obtained using conventional techniques known to those with skill in the art, and the fragments are screened for utility in the same manner as are intact antibodies. An antibody fragment can also be incorporated into single domain antibodies, maxibodies, minibodies, nanobodies, intrabodies, diabodies, triabodies, tetrabodies, v-NAR and bis-scFv (see, for example, Hollinger and Hudson, Nature Biotechnology 23:1126-1136, 2005).

In some embodiments, the encoded antibody of the payload of an AAV particle described herein comprises a multispecific antibody, e.g., it comprises a plurality of immunoglobulin variable domains sequences, wherein a first immunoglobulin variable domain sequence of the plurality has binding specificity for a first epitope and a second immunoglobulin variable domain sequence of the plurality has binding specificity for a second epitope. In some embodiments, the first and second epitopes are on the same antigen, e.g., the same protein (or subunit of a multimeric protein). In some embodiments, the first and second epitopes overlap. In some embodiments, the first and second epitopes do not overlap. In some embodiments, the first and second epitopes are on different antigens, e.g., the different proteins (or different subunits of a multimeric protein). In some embodiments, a multispecific antibody comprises a third, fourth or fifth immunoglobulin variable domain. In some embodiments, a multispecific antibody is a bispecific antibody, a trispecific antibody, or tetraspecific antibody.

In some embodiments, an encoded multispecific antibody of the payload of an AAV particle described herein is an encoded bispecific antibody. A bispecific antibody has specificity for no more than two antigens. A bispecific antibody is characterized by a first immunoglobulin variable domain sequence which has binding specificity for a first epitope and a second immunoglobulin variable domain sequence that has binding specificity for a second epitope. In some embodiments, the first and second epitopes are on the same antigen, e.g., the same protein (or subunit of a multimeric protein). In some embodiments, the first and second epitopes overlap. In some embodiments, the first and second epitopes do not overlap. In some embodiments, the first and second epitopes are on different antigens, e.g., the different proteins (or different subunits of a multimeric protein).

An antibody or an antibody binding fragment encoded in a payload region of an AAV particle described herein, may be, but is not limited to, an antibody or antibody fragment that binds to β-amyloid, APOE, tau, SOD1, TDP-43, huntingtin, and/or synuclein. In some embodiments, the encoded payload comprises an antibody or antibody fragment that binds to a neuro-oncology related target, e.g., HER2, EGFR (e.g., EGFRvIII).

Gene Editing System

In some embodiments, the payload of AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein comprises a gene editing system or one or more components thereof. In some embodiments, the gene editing system comprises nucleic acid sequences that encode proteins having enzymatic activity to (i) selectively induce double or single stranded breaks in a DNA or RNA sequence, or (ii) substitute, insert or delete a particular base or set of bases of a DNA or RNA sequence in the absence of a double or single stranded break in the DNA or RNA. In some embodiments, the gene editing system includes, but is not limited to a CRISPR-Cas system (including different Cas or Cas-related nucleases), a Zinc finger nuclease, a meganuclease, a TALEN or a base editors. In some embodiments, the gene editing system comprises a chromosomal integration of a transgene, e.g., introduced by a parvovirus vector in the absence of an exogenous nuclease or an enzymatic entity.

RNAi Agents

In some embodiments, the payload of AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein comprises an RNAi agent, e.g., an RNAi agent described herein. RNAi (also known as post-transcriptional gene silencing (PTGS), quelling, or co-suppression) is a post-transcriptional gene silencing process in which RNA molecules, in a sequence specific manner, inhibit gene expression, typically by causing the destruction of specific mRNA molecules. RNAi mediated gene silencing can specifically inhibit targeted gene expression. Where the payload region of the viral genome of the AAV particles described herein encodes an RNAi agent, the RNAi agent may be, but is not limited to, dsRNA, siRNA, shRNA, pre-miRNA, pri-miRNA, miRNA, stRNA, lncRNA, piRNA, or snoRNA. Non-limiting examples of a target gene of an RNAi agent include, SOD1, MAPT, APOE, HTT, C9ORF72, TDP-43, APP, BACE, SNCA, ATXN1, ATXN3, ATXN7, SCN1A-SCN5A, or SCN8A-SCN11A.

The AAV particles described herein may comprise viral genomes encoding RNAi agents, wherein the RNAi agent targets the mRNA of a gene of interest to interfere with gene expression and/or protein production. Such AAV particles may be used as a therapeutic, a diagnostic, or for research purposes.

In one embodiment, the RNAi agent may target the gene of interest along any segment of their respective nucleotide sequence.

In one embodiment, the RNAi agent may target the gene of interest at the location of a single-nucleotide polymorphism (SNP) or variant within the nucleotide sequence.

In some embodiments, a nucleic acid sequence encoding an RNAi agent, or a single strand of an RNAi agent, is inserted into the viral genome of the AAV particle and introduced into cells, specifically cells in the central nervous system or cells of the DRG.

The RNAi agent may be an siRNA duplex, wherein the siRNA duplex contains an antisense strand (guide strand) and a sense strand (passenger strand) hybridized together forming a duplex structure, wherein the antisense strand is complementary to the nucleic acid sequence of the targeted gene, and wherein the sense strand is homologous to the nucleic acid sequence of the targeted gene. In some aspects, the 5′ end of the antisense strand has a 5′ phosphate group and the 3′ end of the sense strand contains a 3′ hydroxyl group. In other aspects, there are none, one or 2 nucleotide overhangs at the 3′ end of each strand.

Each strand of an siRNA duplex targeting a gene of interest may be about 19 to 25, 19 to 24 or 19 to 21 nucleotides in length, preferably about 19 nucleotides, 20 nucleotides, 21 nucleotides, 22 nucleotides, 23 nucleotides, 24 nucleotides, or 25 nucleotides in length.

In one embodiment, an siRNA or dsRNA includes at least two sequences that are complementary to each other. The dsRNA includes a sense strand having a first sequence and an antisense strand having a second sequence. The antisense strand includes a nucleotide sequence that is substantially complementary to at least part of an mRNA encoding the target gene, and the region of complementarity is 30 nucleotides or less, and at least 15 nucleotides in length. Generally, the dsRNA is 19 to 25, 19 to 24 or 19 to 21 nucleotides in length. In some embodiments, the dsRNA is from about 15 to about 25 nucleotides in length, and in other embodiments the dsRNA is from about 25 to about 30 nucleotides in length. In some embodiments, the dsRNA is about 15 nucleotides in length, 16 nucleotides in length, 17 nucleotides in length, 18 nucleotides in length, 19 nucleotides, 20 nucleotides, 21 nucleotides, 22 nucleotides, 23 nucleotides, 24 nucleotides, 25 nucleotides in length, 26 nucleotides in length, 27 nucleotides in length, 28 nucleotides in length, 29 nucleotides in length, or 30 nucleotides in length.

The dsRNA, whether directly administered or encoded in a viral genome in an AAV particle, upon contacting with a cell expressing the target protein, inhibits the expression of the protein by at least 10%, at least 20%, at least 25%, at least 30%, at least 35% or at least 40% or more, such as when assayed by a method known in the art.

In one embodiment, the RNAi agent may be used to reduce the expression of target protein by at least about 30%, 40%, 50%, 60%, 70%, 80%, 85%, 90%, 95% and 100%, or at least 20-30%, 20-40%, 20-50%, 20-60%, 20-70%, 20-80%, 20-90%, 20-95%, 20-100%, 30-40%, 30-50%, 30-60%, 30-70%, 30-80%, 30-90%, 30-95%, 30-100%, 40-50%, 40-60%, 40-70%, 40-80%, 40-90%, 40-95%, 40-100%, 50-60%, 50-70%, 50-80%, 50-90%, 50-95%, 50-100%, 60-70%, 60-80%, 60-90%, 60-95%, 60-100%, 70-80%, 70-90%, 70-95%, 70-100%, 80-90%, 80-95%, 80-100%, 90-95%, 90-100% or 95-100%. As a non-limiting example, the expression of target protein expression may be reduced 50-90%.

In one embodiment, the RNAi agent may be used to reduce the expression of target mRNA by at least about 30%, 40%, 50%, 60%, 70%, 80%, 85%, 90%, 95% and 100%, or at least 20-30%, 20-40%, 20-50%, 20-60%, 20-70%, 20-80%, 20-90%, 20-95%, 20-100%, 30-40%, 30-50%, 30-60%, 30-70%, 30-80%, 30-90%, 30-95%, 30-100%, 40-50%, 40-60%, 40-70%, 40-80%, 40-90%, 40-95%, 40-100%, 50-60%, 50-70%, 50-80%, 50-90%, 50-95%, 50-100%, 60-70%, 60-80%, 60-90%, 60-95%, 60-100%, 70-80%, 70-90%, 70-95%, 70-100%, 80-90%, 80-95%, 80-100%, 90-95%, 90-100% or 95-100%. As a non-limiting example, the expression of target mRNA expression may be reduced 50-90%.

In one embodiment, RNAi agent may be used to reduce the expression of target protein and/or mRNA in at least one region of the CNS. The expression of target protein and/or mRNA is reduced by at least about 30%, 40%, 50%, 60%, 70%, 80%, 85%, 90%, 95% and 100%, or at least 20-30%, 20-40%, 20-50%, 20-60%, 20-70%, 20-80%, 20-90%, 20-95%, 20-100%, 30-40%, 30-50%, 30-60%, 30-70%, 30-80%, 30-90%, 30-95%, 30-100%, 40-50%, 40-60%, 40-70%, 40-80%, 40-90%, 40-95%, 40-100%, 50-60%, 50-70%, 50-80%, 50-90%, 50-95%, 50-100%, 60-70%, 60-80%, 60-90%, 60-95%, 60-100%, 70-80%, 70-90%, 70-95%, 70-100%, 80-90%, 80-95%, 80-100%, 90-95%, 90-100% or 95-100% in at least one region of the CNS. As a non-limiting example, the expression of target protein and mRNA in neurons (e.g., cortical neurons) is reduced by 50-90%. As a non-limiting example, the expression of target protein and mRNA in neurons (e.g., cortical neurons) is reduced by 40-50%.

In some embodiments, the AAV particle described herein, comprising a viral genome encoding at least one RNAi agent targeting a gene of interest is administered to a subject in need for treating and/or ameliorating a disease, e.g., a neurological disorder of any disease associated with the central or peripheral nervous systems.

In one embodiment, the RNAi agent is an siRNA.

Design of siRNA

An AAV particle described herein (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant described herein) may comprise a viral genome encoding one or more siRNA molecules (e.g., siRNA duplexes or encoded dsRNA) that are specifically designed to target a gene of interest and suppress target gene expression and protein production. In some aspects, the siRNA molecules are designed and used to selectively “knock out” target gene variants in cells, e.g., transcripts that are identified in neurological disease. In some aspects, the siRNA molecules are designed and used to selectively “knock down” target gene variants in cells.

In some embodiments, siRNA molecules targeting a gene of interest may be designed using any available design tools.

Some guidelines for designing siRNAs (for insertion into a viral genome of the AAV particles described herein) have been proposed in the art. These guidelines generally recommend generating a 19-nucleotide duplexed region, symmetric 2-3 nucleotide 3′ overhangs, 5-phosphate and 3-hydroxyl groups targeting a region in the gene to be silenced. Other rules that may govern siRNA sequence preference include, but are not limited to, (i) A/U at the 5′ end of the antisense strand; (ii) G/C at the 5′ end of the sense strand; (iii) at least five A/U residues in the 5′ terminal one-third of the antisense strand; and (iv) the absence of any GC stretch of more than 9 nucleotides in length. In accordance with such considerations, together with the specific sequence of a target gene, highly effective siRNA molecules essential for suppressing mammalian target gene expression may be readily designed.

In one embodiment, the sense and/or antisense strand is designed based on the method and rules outlined in European Patent Publication No. EP1752536, the contents of which are herein incorporated by reference in their entirety. As a non-limiting example, the 3′-terminal base of the sequence is adenine, thymine or uracil. As a non-limiting example, the 5′-terminal base of the sequence is guanine or cytosine. As a non-limiting example, the 3′-terminal sequence comprises seven bases rich in one or more bases of adenine, thymine and uracil.

In one embodiment, an siRNA molecule comprises a sense strand and a complementary antisense strand in which both strands are hybridized together to form a duplex structure. The antisense strand has sufficient complementarity to the target mRNA sequence to direct target-specific RNAi, e.g., the siRNA molecule has a sequence sufficient to trigger the destruction of the target mRNA by the RNAi machinery or process.

In some embodiments, the antisense strand and target mRNA sequences have 100% complementarity. The antisense strand may be complementary to any part of the target mRNA sequence. Neither the identity of the sense sequence nor the homology of the antisense sequence need be 100% complementary to the target.

In other embodiments, the antisense strand and target mRNA sequences comprise at least one mismatch. As a non-limiting example, the antisense strand and the target mRNA sequence have at least 30%, 40%, 50%, 60%, 70%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% or at least 20-30%, 20-40%, 20-50%, 20-60%, 20-70%, 20-80%, 20-90%, 20-95%, 20-99%, 30-40%, 30-50%, 30-60%, 30-70%, 30-80%, 30-90%, 30-95%, 30-99%, 40-50%, 40-60%, 40-70%, 40-80%, 40-90%, 40-95%, 40-99%, 50-60%, 50-70%, 50-80%, 50-90%, 50-95%, 50-99%, 60-70%, 60-80%, 60-90%, 60-95%, 60-99%, 70-80%, 70-90%, 70-95%, 70-99%, 80-90%, 80-95%, 80-99%, 90-95%, 90-99% or 95-99% complementary.

The siRNA molecule may have a length from about 10-50 or more nucleotides, e.g., each strand comprising 10-50 nucleotides (or nucleotide analogs). Preferably, the siRNA molecule has a length from about 15-30, e.g., 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 nucleotides in each strand, wherein one of the strands is sufficiently complementary to a target region. In one embodiment, the siRNA molecule has a length from about 19 to 25, 19 to 24 or 19 to 21 nucleotides.

In some embodiments, the siRNA molecule can be a synthetic RNA duplex comprising about 19 nucleotides to about 25 nucleotides, and two overhanging nucleotides at the 3′-end.

The siRNA molecule may comprise an antisense sequence and a sense sequence, or a fragment or variant thereof. As a non-limiting example, the antisense sequence and the sense sequence have at least 30%, 40%, 50%, 60%, 70%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% or at least 20-30%, 20-40%, 20-50%, 20-60%, 20-70%, 20-80%, 20-90%, 20-95%, 20-99%, 30-40%, 30-50%, 30-60%, 30-70%, 30-80%, 30-90%, 30-95%, 30-99%, 40-50%, 40-60%, 40-70%, 40-80%, 40-90%, 40-95%, 40-99%, 50-60%, 50-70%, 50-80%, 50-90%, 50-95%, 50-99%, 60-70%, 60-80%, 60-90%, 60-95%, 60-99%, 70-80%, 70-90%, 70-95%, 70-99%, 80-90%, 80-95%, 80-99%, 90-95%, 90-99% or 95-99% complementary.

The sense and antisense sequences may be completely complementary across a substantial portion of their length. In other embodiments, the sense sequence and antisense sequence may be at least 70, 80, 90, 95 or 99% complementary across independently at least 50, 60, 70, 80, 85, 90, 95, or 99% of the length of the strands.

In some embodiments, the sense and antisense strands of a siRNA duplex are linked by a short spacer sequence leading to the expression of a stem-loop structure termed short hairpin RNA (shRNA). The hairpin is recognized and cleaved by Dicer, thus generating mature siRNA molecules.

In some embodiments, the siRNA molecules, as well as associated spacer and/or flanking regions once designed, can be encoded by the viral genome of the AAV particles described herein, for delivery to a cell.

Molecular Scaffold

In some embodiments, the siRNA molecules may be encoded in a modulatory polynucleotide which also comprises a molecular scaffold.

In some embodiments, the modulatory polynucleotide which comprises the payload (e.g., siRNA, miRNA or other RNAi agent described herein) includes a molecular scaffold which comprises at least one 5′ flanking sequence which may be of any length and may be derived in whole or in part from wild type microRNA sequence or be completely artificial. A 3′ flanking sequence may mirror the 5′ flanking sequence in size and origin. Either flanking sequence may be absent. In one embodiment, both the 5′ and 3′ flanking sequences are absent. The 3′ flanking sequence may optionally contain one or more CNNC motifs, where “N” represents any nucleotide.

In some embodiments the 5′ and 3′ flanking sequences are the same length.

In some embodiments the 5′ flanking sequence is from 1-10 nucleotides in length, from 5-15 nucleotides in length, from 10-30 nucleotides in length, from 20-50 nucleotides in length, greater than 40 nucleotides in length, greater than 50 nucleotides in length, greater than 100 nucleotides in length or greater than 200 nucleotides in length.

In some embodiments, the 5′ flanking sequence may be 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462, 463, 464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476, 477, 478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490, 491, 492, 493, 494, 495, 496, 497, 498, 499, or 500 nucleotides in length.

In one embodiment, the molecular scaffold comprises at least one 3′ flanking region. As a non-limiting example, the 3′ flanking region may comprise a 3′ flanking sequence which may be of any length and may be derived in whole or in part from wild type microRNA sequence or be a completely artificial sequence.

In some embodiments the 3′ flanking sequence is from 1-10 nucleotides in length, from 5-15 nucleotides in length, from 10-30 nucleotides in length, from 20-50 nucleotides in length, greater than 40 nucleotides in length, greater than 50 nucleotides in length, greater than 100 nucleotides in length or greater than 200 nucleotides in length.

In some embodiments, the 3′ flanking sequence may be 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462, 463, 464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476, 477, 478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490, 491, 492, 493, 494, 495, 496, 497, 498, 499, or 500 nucleotides in length.

In some embodiments the 5′ and 3′ flanking sequences are the same sequence. In some embodiments they differ by 2%, 3%, 4%, 5%, 10%, 20% or more than 30% when aligned to each other.

Forming the stem of a stem loop structure is a minimum of at least one payload sequence. In some embodiments, the payload sequence comprises at least one nucleic acid sequence which is in part complementary or will hybridize to the target sequence. In some embodiments, the payload is an siRNA molecule or fragment of an siRNA molecule.

In some embodiments, the 5′ arm of the stem loop comprises a sense sequence.

In some embodiments, the 3′ arm of the stem loop comprises an antisense sequence. The antisense sequence, in some instances, comprises a “G” nucleotide at the 5′ most end.

In other embodiments, the sense sequence may reside on the 3′ arm while the antisense sequence resides on the 5′ arm of the stem of the stem loop structure.

Separating the sense and antisense sequence of the stem loop structure is a loop (also known as a loop motif). The loop may be of any length, between 4-30 nucleotides, between 4-20 nucleotides, between 4-15 nucleotides, between 5-15 nucleotides, between 6-12 nucleotides, 6 nucleotides, 7, nucleotides, 8 nucleotides, 9 nucleotides, 10 nucleotides, 11 nucleotides, and/or 12 nucleotides.

In some embodiments, the loop comprises at least one UGUG motif. In some embodiments, the UGUG motif is located at the 5′ terminus of the loop.

Spacer regions may be present in the modulatory polynucleotide to separate one or more modules from one another. There may be one or more such spacer regions present.

In one embodiment, a spacer region of between 8-20, e.g., 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 nucleotides may be present between the sense sequence and a flanking sequence.

In one embodiment, the spacer is 13 nucleotides and is located between the 5′ terminus of the sense sequence and a flanking sequence. In one embodiment, a spacer is of sufficient length to form approximately one helical turn of the sequence.

In one embodiment, a spacer region of between 8-20, e.g., 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 nucleotides may be present between the antisense sequence and a flanking sequence.

In one embodiment, the spacer sequence is between 10-13, e.g., 10, 11, 12 or 13 nucleotides and is located between the 3′ terminus of the antisense sequence and a flanking sequence. In one embodiment, a spacer is of sufficient length to form approximately one helical turn of the sequence.

In one embodiment, the modulatory polynucleotide comprises in the 5′ to 3′ direction, a 5′ flanking sequence, a 5′ arm, a loop motif, a 3′ arm and a 3′ flanking sequence. As a non-limiting example, the 5′ arm may comprise a sense sequence and the 3′ arm comprises the antisense sequence. In another non-limiting example, the 5′ arm comprises the antisense sequence and the 3′ arm comprises the sense sequence.

In one embodiment, the 5′ arm, payload (e.g., sense and/or antisense sequence), loop motif and/or 3′ arm sequence may be altered (e.g., substituting 1 or more nucleotides, adding nucleotides and/or deleting nucleotides). The alteration may cause a beneficial change in the function of the construct (e.g., increase knock-down of the target sequence, reduce degradation of the construct, reduce off target effect, increase efficiency of the payload, and reduce degradation of the payload).

In one embodiment, the molecular scaffold of the modulatory polynucleotides is aligned in order to have the rate of excision of the guide or antisense strand be greater than the rate of excision of the passenger or sense strand. The rate of excision of the guide or passenger strand may be, independently, 1%, 2%, 3%, 4%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 99% or more than 99%. As a non-limiting example, the rate of excision of the guide strand is at least 80%. As another non-limiting example, the rate of excision of the guide strand is at least 90%.

In one embodiment, the rate of excision of the guide strand is greater than the rate of excision of the passenger strand. In one aspect, the rate of excision of the guide strand may be at least 1%, 2%, 3%, 4%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 99% or more than 99% greater than the passenger strand.

In one embodiment, the efficiency of excision of the guide strand is at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 99% or more than 99%. As a non-limiting example, the efficiency of the excision of the guide strand is greater than 80%.

In one embodiment, the efficiency of the excision of the guide strand is greater than the excision of the passenger strand from the molecular scaffold. The excision of the guide strand may be 2, 3, 4, 5, 6, 7, 8, 9, 10 or more than 10 times more efficient than the excision of the passenger strand from the molecular scaffold.

In one embodiment, the molecular scaffold comprises a dual-function targeting modulatory polynucleotide.

In one embodiment, the molecular scaffold may comprise one or more linkers known in the art. The linkers may separate regions or one molecular scaffold from another. As a non-limiting example, the molecular scaffold may be polycistronic.

In one embodiment, the modulatory polynucleotide is designed using at least one of the following properties: loop variant, seed mismatch/bulge/wobble variant, stem mismatch, loop variant and basal stem mismatch variant, seed mismatch and basal stem mismatch variant, stem mismatch and basal stem mismatch variant, seed wobble and basal stem wobble variant, or a stem sequence variant.

AAV Production

Viral production disclosed herein describes processes and methods for producing AAV particles (with enhanced, improved and/or increased tropism for a target tissue), e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant, that may be used to contact a target cell to deliver a payload.

In some embodiments, disclosed herein is a method of making AAV particle of the present disclosure, e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant, the method comprising: (i) providing a host cell comprising a viral genome described herein and (ii) incubating the host cell under conditions suitable to enclose the viral genome in an AAV capsid variant, e.g., an AAV capsid variant described herein (e.g., an AAV capsid variant listed in Tables 3, 4, or 5), thereby making the AAV particle. In some embodiments, the method comprises prior to step (i), introducing a first nucleic acid comprising the viral genome into a cell. In some embodiments, the host cell comprises a second nucleic acid encoding the AAV capsid variant. In some embodiments, the second nucleic acid is introduced into the host cell prior to, concurrently with, or after the first nucleic acid molecule. In some embodiments, the AAV particle described herein is an isolated AAV particle. In some embodiments, the AAV particle described herein is a recombinant AAV particle.

The present disclosure provides methods for the generation of an AAV particle comprising a peptide, e.g., a targeting peptide, (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant, described herein). In some embodiments, the AAV particles are prepared by viral genome replication in a viral replication cell. Any method known in the art may be used for the preparation of AAV particles. In some embodiments, AAV particles are produced in mammalian cells (e.g., HEK293). In another embodiment, AAV particles are produced in insect cells (e.g., Sf9).

Methods of making AAV particles are well known in the art and are described in e.g., U.S. Pat. Nos. 6,204,059, 5,756,283, 6,258,595, 6,261,551, 6,270,996, 6,281,010, 6,365,394, 6,475,769, 6,482,634, 6,485,966, 6,943,019, 6,953,690, 7,022,519, 7,238,526, 7,291,498 and 7,491,508, 5,064,764, 6,194,191, 6,566,118, 8,137,948; or International Publication Nos. WO1996039530, WO1998010088, WO1999014354, WO1999015685, WO1999047691, WO2000055342, WO2000075353 and WO2001023597; Methods In Molecular Biology, ed. Richard, Humana Press, N J (1995); O'Reilly et al., Baculovirus Expression Vectors, A Laboratory Manual, Oxford Univ. Press (1994); Samulski et al., J. Vir. 63:3822-8 (1989); Kajigaya et al., Proc. Nat'l. Acad. Sci. USA 88: 4646-50 (1991); Ruffing et al., J. Vir. 66:6922-30 (1992); Kimbauer et al., Vir., 219:37-44 (1996); Zhao et al., Vir. 272:382-93 (2000); the contents of each of which are herein incorporated by reference in their entirety. In some embodiments, the AAV particles are made using the methods described in International Patent Publication WO2015191508, the contents of which are herein incorporated by reference in their entirety.

Therapeutic Applications

The present disclosure provides a method for treating a disease, disorder and/or condition in a mammalian subject, including a human subject, comprising administering to the subject an AAV particle described herein, e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant (e.g., an AAV capsid variant described herein), or administering to the subject any of the described compositions, including a pharmaceutical composition, described herein.

In some embodiments, the AAV particle of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) are administered to a subject prophylactically, to prevent on-set of disease. In another embodiment, the AAV particle (e.g., an AAV particle comprising an AAV capsid variant) of the present disclosure are administered to treat (lessen the effects of) a disease or symptoms thereof. In yet another embodiment, the AAV particle of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) are administered to cure (eliminate) a disease. In another embodiment, the AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) of the present disclosure are administered to prevent or slow progression of disease. In yet another embodiment, the AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) of the present disclosure are used to reverse the deleterious effects of a disease. Disease status and/or progression may be determined or monitored by standard methods known in the art.

In some embodiments, provided herein is method for treating a neurological disorder and/or neurodegenerative disorder in a subject, comprising administering to the subject an effective amount of a pharmaceutical composition described herein or an AAV particle, e.g., a plurality of particles, comprising an AAV capsid variant described herein. In some embodiments, treatment of a neurological disorder and/or neurodegenerative disorder comprises prevention of said neurological disorder and/or neurological disorder.

In some embodiments, the AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) of the disclosure is useful in the field of medicine for the treatment, prophylaxis, palliation or amelioration of neurological diseases and/or disorders.

In some embodiments, the AAV particle of the disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) is useful in the field of medicine for the treatment, prophylaxis, palliation or amelioration of tauopathy.

In some embodiments, the AAV particle of the disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) is useful in the field of medicine for the treatment, prophylaxis, palliation or amelioration of Alzheimer's Disease.

In some embodiments, the AAV particle of the disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) is useful in the field of medicine for the treatment, prophylaxis, palliation or amelioration of Friedreich's ataxia, or any disease stemming from a loss or partial loss of frataxin protein.

In some embodiments, the AAV particle of the disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) is useful in the field of medicine for the treatment, prophylaxis, palliation or amelioration of Parkinson's Disease.

In some embodiments, the AAV particle of the disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) is useful in the field of medicine for the treatment, prophylaxis, palliation or amelioration of Amyotrophic lateral sclerosis.

In some embodiments, the AAV particle of the disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) is useful in the field of medicine for the treatment, prophylaxis, palliation or amelioration of Huntington's Disease.

In some embodiments, the AAV particle of the disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) is useful in the field of medicine for the treatment, prophylaxis, palliation or amelioration of chronic or neuropathic pain.

In some embodiments, the AAV particle of the disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) is useful in the field of medicine for treatment, prophylaxis, palliation or amelioration of a disease associated with the central nervous system.

In some embodiments, the AAV particle of the disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) is useful in the field of medicine for treatment, prophylaxis, palliation or amelioration of a disease associated with the peripheral nervous system.

In some embodiments, the AAV particle of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) is administered to a subject having at least one of the diseases or symptoms described herein. In some embodiments, an AAV particle of the present disclosure is administered to a subject having or diagnosed with having a disease or disorder described herein.

In some embodiments, provided herein is a method for treating a muscular disorder and/or neuromuscular disorder in a subject, comprising administering to the subject an effective amount of a pharmaceutical composition described herein or an AAV particle, e.g., a plurality of particles, comprising an AAV capsid variant described herein. In some embodiments, treatment of a muscular disorder and/or neuromuscular disorder comprises prevention of said muscular disorder and/or neuromuscular disorder.

In some embodiments, provided herein is a method for treating a neuro-oncological disorder in a subject, comprising administering to the subject an effective amount of a pharmaceutical composition described herein or an AAV particle, e.g., a plurality of particles, comprising an AAV capsid variant described herein. In some embodiments, treatment of a neuro-oncological disorder comprises prevention of said neuro-oncological disorder. In some embodiments, a neuro-oncological disorder comprises a cancer of a primary CNS origin (e.g., a CNS cell, a tissue, or a region), or a metastatic cancer in a CNS cell, tissue, or region.

Any neurological disease or disorder, neurodegenerative disorder, muscular disorder, neuromuscular disorder, and/or neuro-oncological disorder may be treated with the AAV particles of the disclosure, or pharmaceutical compositions thereof, including but not limited to, Absence of the Septum Pellucidum, Acid Lipase Disease, Acid Maltase Deficiency, Acquired Epileptiform Aphasia, Acute Disseminated Encephalomyelitis, Attention Deficit-Hyperactivity Disorder (ADHD), Adie's Pupil, Adie's Syndrome, Adrenoleukodystrophy, Agenesis of the Corpus Callosum, Agnosia, Aicardi Syndrome, Aicardi-Goutieres Syndrome Disorder, AIDS—Neurological Complications, Alexander Disease, Alpers' Disease, Alternating Hemiplegia, Alzheimer's Disease, Amyotrophic Lateral Sclerosis (ALS), Anencephaly, Aneurysm, Angelman Syndrome, Angiomatosis, Anoxia, Antiphospholipid Syndrome, Aphasia, Apraxia, Arachnoid Cysts, Arachnoiditis, Arnold-Chiari Malformation, Arteriovenous Malformation, Asperger Syndrome, Ataxia, Ataxia Telangiectasia, Ataxias and Cerebellar or Spinocerebellar Degeneration, Atrial Fibrillation and Stroke, Attention Deficit-Hyperactivity Disorder, Autism Spectrum Disorder, Autonomic Dysfunction, Back Pain, Barth Syndrome, Batten Disease, Becker's Myotonia, Bechet's Disease, Bell's Palsy, Benign Essential Blepharospasm, Benign Focal Amyotrophy, Benign Intracranial Hypertension, Bernhardt-Roth Syndrome, Binswanger's Disease, Blepharospasm, Bloch-Sulzberger Syndrome, Brachial Plexus Birth Injuries, Brachial Plexus Injuries, Bradbury-Eggleston Syndrome, Brain and Spinal Tumors, Brain Aneurysm, Brain Injury, Brown-Sequard Syndrome, Bulbar palsy, Bulbospinal Muscular Atrophy, Cerebral Autosomal Dominant Arteriopathy with Sub-cortical Infarcts and Leukoencephalopathy (CADASIL), Canavan Disease, Carpal Tunnel Syndrome, Causalgia, Cavernomas, Cavernous Angioma, Cavernous Malformation, Central Cervical Cord Syndrome, Central Cord Syndrome, Central Pain Syndrome, Central Pontine Myelinolysis, Cephalic Disorders, Ceramidase Deficiency, Cerebellar Degeneration, Cerebellar Hypoplasia, Cerebral Aneurysms, Cerebral Arteriosclerosis, Cerebral Atrophy, Cerebral Beriberi, Cerebral Cavernous Malformation, Cerebral Gigantism, Cerebral Hypoxia, Cerebral Palsy, Cerebro-Oculo-Facio-Skeletal Syndrome (COFS), Charcot-Marie-Tooth Disease, Chiari Malformation, Cholesterol Ester Storage Disease, Chorea, Choreoacanthocytosis, Chronic Inflammatory Demyelinating Polyneuropathy (CIDP), Chronic Orthostatic Intolerance, Chronic Pain, Cockayne Syndrome Type II, Coffin Lowry Syndrome, Colpocephaly, Coma, Complex Regional Pain Syndrome, Concentric sclerosis (Baló's sclerosis), Congenital Facial Diplegia, Congenital Myasthenia, Congenital Myopathy, Congenital Vascular Cavernous Malformations, Corticobasal Degeneration, Cranial Arteritis, Craniosynostosis, Cree encephalitis, Creutzfeldt-Jakob Disease, Chronic progressive external ophtalmoplegia, Cumulative Trauma Disorders, Cushing's Syndrome, Cytomegalic Inclusion Body Disease, Cytomegalovirus Infection, Dancing Eyes-Dancing Feet Syndrome, Dandy-Walker Syndrome, Dawson Disease, De Morsier's Syndrome, Dejerine-Klumpke Palsy, Dementia, Dementia—Multi-Infarct, Dementia—Semantic, Dementia—Subcortical, Dementia With Lewy Bodies, Demyelination diseases, Dentate Cerebellar Ataxia, Dentatorubral Atrophy, Dermatomyositis, Developmental Dyspraxia, Devic's Syndrome, Diabetic Neuropathy, Diffuse Sclerosis, Distal hereditary motor neuronopathies, Dravet Syndrome, Dysautonomia, Dysgraphia, Dyslexia, Dysphagia, Dyspraxia, Dyssynergia Cerebellaris Myoclonica, Dyssynergia Cerebellaris Progressiva, Dystonias, Early Infantile Epileptic Encephalopathy, Empty Sella Syndrome, Encephalitis, Encephalitis Lethargica, Encephaloceles, Encephalomyelitis, Encephalopathy, Encephalopathy (familial infantile), Encephalotrigeminal Angiomatosis, Epilepsy, Epileptic Hemiplegia, Episodic ataxia, Erb's Palsy, Erb-Duchenne and Dejerine-Klumpke Palsies, Essential Tremor, Extrapontine Myelinolysis, Faber's disease, Fabry Disease, Fahr's Syndrome, Fainting, Familial Dysautonomia, Familial Hemangioma, Familial Idiopathic Basal Ganglia Calcification, Familial Periodic Paralyses, Familial Spastic Paralysis, Farber's Disease, Febrile Seizures, Fibromuscular Dysplasia, Fisher Syndrome, Floppy Infant Syndrome, Foot Drop, Friedreich's Ataxia, Frontotemporal Dementia, Gaucher Disease, Generalized Gangliosidoses (GM1, GM2), Gerstmann's Syndrome, Gerstmann-Straussler-Scheinker Disease, Giant Axonal Neuropathy, Giant Cell Arteritis, Giant Cell Inclusion Disease, Globoid Cell Leukodystrophy, Glossopharyngeal Neuralgia, Glycogen Storage Disease, Guillain-Barré Syndrome, Hallervorden-Spatz Disease, Head Injury, Headache, Hemicrania Continua, Hemifacial Spasm, Hemiplegia Alterans, Hereditary Neuropathies, Hereditary Spastic Paraplegia, Heredopathia Atactica Polyneuritiformis, Herpes Zoster, Herpes Zoster Oticus, Hirayama Syndrome, Holmes-Adie syndrome, Holoprosencephaly, HTLV-1 Associated Myelopathy, Hughes Syndrome, Huntington's Disease, Hurler syndrome, Hydranencephaly, Hydrocephalus, Hydrocephalus—Normal Pressure, Hydromyelia, Hypercortisolism, Hypersomnia, Hypertonia, Hypotonia, Hypoxia, Immune-Mediated Encephalomyelitis, Inclusion Body Myositis, Incontinentia Pigmenti, Infantile Hypotonia, Infantile Neuroaxonal Dystrophy, Infantile Phytanic Acid Storage Disease, Infantile Refsum Disease, Infantile Spasms, Inflammatory Myopathies, Iniencephaly, Intestinal Lipodystrophy, Intracranial Cysts, Intracranial Hypertension, Isaacs' Syndrome, Joubert Syndrome, Kearns-Sayre Syndrome, Kennedy's Disease, Kinsbourne syndrome, Kleine-Levin Syndrome, Klippel-Feil Syndrome, Klippel-Trenaunay Syndrome (KTS), Klüver-Bucy Syndrome, Korsakoffs Amnesic Syndrome, Krabbe Disease, Kugelberg-Welander Disease, Kuru, Lambert-Eaton Myasthenic Syndrome, Landau-Kleffner Syndrome, Lateral Femoral Cutaneous Nerve Entrapment, Lateral Medullary Syndrome, Learning Disabilities, Leigh's Disease, Lennox-Gastaut Syndrome, Lesch-Nyhan Syndrome, Leukodystrophy, Levine-Critchley Syndrome, Lewy Body Dementia, Lichtheim's disease, Lipid Storage Diseases, Lipoid Proteinosis, Lissencephaly, Locked-In Syndrome, Lou Gehrig's Disease, Lupus—Neurological Sequelae, Lyme Disease—Neurological Complications, Lysosomal storage disorders, Machado-Joseph Disease, Macrencephaly, Megalencephaly, Melkersson-Rosenthal Syndrome, Meningitis, Meningitis and Encephalitis, Menkes Disease, Meralgia Paresthetica, Metachromatic Leukodystrophy, Microcephaly, Migraine, Miller Fisher Syndrome, Mini Stroke, Mitochondrial Myopathy, Mitochondrial DNA depletion syndromes, Moebius Syndrome, Monomelic Amyotrophy, Morvan Syndrome, Motor Neuron Diseases, Moyamoya Disease, Mucolipidoses, Mucopolysaccharidoses, Multi-Infarct Dementia, Multifocal Motor Neuropathy, Multiple Sclerosis, Multiple System Atrophy, Multiple System Atrophy with Orthostatic Hypotension, Muscular Dystrophy, Myasthenia—Congenital, Myasthenia Gravis, Myelinoclastic Diffuse Sclerosis, Myelitis, Myoclonic Encephalopathy of Infants, Myoclonus, Myoclonus epilepsy, Myopathy, Myopathy-Congenital, Myopathy—Thyrotoxic, Myotonia, Myotonia Congenita, Narcolepsy, NARP (neuropathy, ataxia and retinitis pigmentosa), Neuroacanthocytosis, Neurodegeneration with Brain Iron Accumulation, Neurodegenerative disease, Neurofibromatosis, Neuroleptic Malignant Syndrome, Neurological Complications of AIDS, Neurological Complications of Lyme Disease, Neurological Consequences of Cytomegalovirus Infection, Neurological Manifestations of Pompe Disease, Neurological Sequelae Of Lupus, Neuromyelitis Optica, Neuromyotonia, Neuronal Ceroid Lipofuscinosis, Neuronal Migration Disorders, Neuropathic pain, Neuropathy—Hereditary, Neuropathy, Neurosarcoidosis, Neurosyphilis, Neurotoxicity, Nevus Cavernosus, Niemann-Pick Disease, O'Sullivan-McLeod Syndrome, Occipital Neuralgia, Ohtahara Syndrome, Olivopontocerebellar Atrophy, Opsoclonus Myoclonus, Orthostatic Hypotension, Overuse Syndrome, Pain—Chronic, Pantothenate Kinase-Associated Neurodegeneration, Paraneoplastic Syndromes, Paresthesia, Parkinson's Disease, Paroxysmal Choreoathetosis, Paroxysmal Hemicrania, Parry-Romberg, Pelizaeus-Merzbacher Disease, Pena Shokeir II Syndrome, Perineural Cysts, Peroneal muscular atrophy, Periodic Paralyses, Peripheral Neuropathy, Periventricular Leukomalacia, Persistent Vegetative State, Pervasive Developmental Disorders, Phytanic Acid Storage Disease, Pick's Disease, Pinched Nerve, Piriformis Syndrome, Pituitary Tumors, Polymyositis, Pompe Disease, Porencephaly, Post-Polio Syndrome, Postherpetic Neuralgia, Postinfectious Encephalomyelitis, Postural Hypotension, Postural Orthostatic Tachycardia Syndrome, Postural Tachycardia Syndrome, Primary Dentatum Atrophy, Primary Lateral Sclerosis, Primary Progressive Aphasia, Prion Diseases, Progressive bulbar palsy, Progressive Hemifacial Atrophy, Progressive Locomotor Ataxia, Progressive Multifocal Leukoencephalopathy, Progressive Muscular Atrophy, Progressive Sclerosing Poliodystrophy, Progressive Supranuclear Palsy, Prosopagnosia, Pseudobulbar palsy, Pseudo-Torch syndrome, Pseudotoxoplasmosis syndrome, Pseudotumor Cerebri, Psychogenic Movement, Ramsay Hunt Syndrome I, Ramsay Hunt Syndrome II, Rasmussen's Encephalitis, Reflex Sympathetic Dystrophy Syndrome, Refsum Disease, Refsum Disease—Infantile, Repetitive Motion Disorders, Repetitive Stress Injuries, Restless Legs Syndrome, Retrovirus-Associated Myelopathy, Rett Syndrome, Reye's Syndrome, Rheumatic Encephalitis, Riley-Day Syndrome, Sacral Nerve Root Cysts, Saint Vitus Dance, Salivary Gland Disease, Sandhoff Disease, Schilder's Disease, Schizencephaly, Seitelberger Disease, Seizure Disorder, Semantic Dementia, Septo-Optic Dysplasia, Severe Myoclonic Epilepsy of Infancy (SMEI), Shaken Baby Syndrome, Shingles, Shy-Drager Syndrome, Sjögren's Syndrome, Sleep Apnea, Sleeping Sickness, Sotos Syndrome, Spasticity, Spina Bifida, Spinal Cord Infarction, Spinal Cord Injury, Spinal Cord Tumors, Spinal Muscular Atrophy, Spinocerebellar Ataxia, Spinocerebellar Atrophy, Spinocerebellar Degeneration, Sporadic ataxia, Steele-Richardson-Olszewski Syndrome, Stiff-Person Syndrome, Striatonigral Degeneration, Stroke, Sturge-Weber Syndrome, Subacute Sclerosing Panencephalitis, Subcortical Arteriosclerotic Encephalopathy, Short-lasting, Unilateral, Neuralgiform (SUNCT) Headache, Swallowing Disorders, Sydenham Chorea, Syncope, Syphilitic Spinal Sclerosis, Syringohydromyelia, Syringomyelia, Systemic Lupus Erythematosus, Tabes Dorsalis, Tardive Dyskinesia, Tarlov Cysts, Tay-Sachs Disease, Temporal Arteritis, Tethered Spinal Cord Syndrome, Thomsen's Myotonia, Thoracic Outlet Syndrome, Thyrotoxic Myopathy, Tic Douloureux, Todd's Paralysis, Tourette Syndrome, Transient Ischemic Attack, Transmissible Spongiform Encephalopathies, Transverse Myelitis, Traumatic Brain Injury, Tremor, Trigeminal Neuralgia, Tropical Spastic Paraparesis, Troyer Syndrome, Tuberous Sclerosis, Vascular Erectile Tumor, Vasculitis Syndromes of the Central and Peripheral Nervous Systems, Vitamin B12 deficiency, Von Economo's Disease, Von Hippel-Lindau Disease (VHL), Von Recklinghausen's Disease, Wallenberg's Syndrome, Werdnig-Hoffman Disease, Wernicke-Korsakoff Syndrome, West Syndrome, Whiplash, Whipple's Disease, Williams Syndrome, Wilson Disease, Wolman's Disease, X-Linked Spinal and Bulbar Muscular Atrophy.

Pharmaceutical Composition and Formulations

According to the present disclosure, an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant described herein may be prepared as a pharmaceutical composition. In some embodiments, the pharmaceutical composition comprises at least one active ingredients. In some embodiments, the pharmaceutical composition comprises a pharmaceutically acceptable excipient.

In some embodiments, an AAV particle of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid polypeptide, e.g., an AAV capsid variant) can be formulated using an excipient to: (1) increase stability; (2) increase cell transfection or transduction; (3) permit the sustained or delayed expression of the payload; (4) alter the biodistribution (e.g., target the viral particle to specific tissues or cell types); (5) increase the translation of encoded protein; (6) alter the release profile of encoded protein; and/or (7) allow for regulatable expression of the payload. Formulations of the present disclosure can include, without limitation, saline, liposomes, lipid nanoparticles, polymers, peptides, proteins, cells transfected with viral vectors (e.g., for transfer or transplantation into a subject) and combinations thereof.

In some embodiments, the relative amount of the active ingredient (e.g. an AAV particle described herein), a pharmaceutically acceptable excipient, and/or any additional ingredients in a pharmaceutical composition in accordance with the present disclosure may vary, depending upon the identity, size, and/or condition of the subject being treated and further depending upon the route by which the composition is to be administered. For example, the composition may comprise between 0.1% and 99% (w/w) of the active ingredient. By way of example, the composition may comprise between 0.1% and 100%, e.g., between 0.5 and 50%, between 1-30%, between 5-80%, at least 80% (w/w) active ingredient.

In some embodiments, the pharmaceutical composition comprising an AAV particle described herein may comprise an AAV capsid polypeptide, e.g., an AAV capsid variant and a viral genome encoding a payload, e.g., a payload described herein, with or without a pharmaceutically acceptable excipient.

The present disclosure also provides in some embodiments, a pharmaceutical composition suitable for administration to a subject, e.g., a human. In some embodiments, the pharmaceutical composition is administered to a subject, e.g., a human.

Administration

In some embodiments, an AAV particle disclosed herein (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) may be administered by a to a subject by a delivery route, e.g., a localized delivery route or a systemic delivery route. In some embodiments, the AAV particle is administered to a subject by a delivery route which results in therapeutically effective outcome.

In some embodiments, an AAV particle described herein (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) may be administered via such a route that it is able to cross the blood-brain barrier, vascular barrier, or other epithelial barrier. In some embodiments, an AAV particle of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) may be administered in any suitable form, either as a liquid solution or suspension, as a solid form suitable for liquid solution or suspension in a liquid solution. In some embodiments, an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) may be formulated with any appropriate and pharmaceutically acceptable excipient.

In some embodiments, the AAV particle described herein (e.g., an AAV particle comprising, an AAV capsid polypeptide, e.g., an AAV capsid variant) is administered intramuscularly, intravenously, intracerebrally, intrathecally, intracerebroventricularly, via intraparenchymal administration, or via intra-cisterna magna injection (ICM).

In some embodiments, an AAV particle of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) may be delivered to a subject via a single route administration. In some embodiments, an AAV particle of the present disclosure may be delivered to a subject via a multi-site route of administration. In some embodiments, a subject may be administered at 2, 3, 4, 5, or more than 5 sites.

In some embodiments, an AAV particle of the present disclosure (e.g., an AAV particle comprising, an AAV capsid polypeptide, e.g., an AAV capsid variant) is administered via a bolus infusion. In some embodiments, an AAV particle of the present disclosure is administered via sustained delivery over a period of minutes, hours, or days. In some embodiments, the infusion rate may be changed depending on the subject, distribution, formulation, and/or another delivery parameter. In some embodiments, an AAV particle of the present disclosure is administered using a controlled release, e.g., a release profile that conforms to a particular pattern of release to effect a therapeutic outcome. In some embodiments, an AAV particle of the present disclosure is administered using a sustained release, e.g., a release profile that conforms to a release rate over a specific period of time.

In some embodiments, an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) may be delivered by more than one route of administration. As non-limiting examples of combination administrations, an AAV particle may be delivered by intrathecal and intracerebroventricular, or by intravenous and intraparenchymal administration.

Intravenous Administration

In some embodiments, an AAV particle described herein (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) may be administered to a subject by systemic administration. In some embodiments, the systemic administration is intravenous administration. In another embodiment, the systemic administration is intraarterial administration. In some embodiments, an AAV particle of the present disclosure may be administered to a subject by intravenous administration. In some embodiments, the intravenous administration may be achieved by subcutaneous delivery. In some embodiments, the AAV particle is administered to the subject via focused ultrasound (FUS), e.g., coupled with the intravenous administration of microbubbles (FUS-MB) or MRI-guided FUS coupled with intravenous administration, e.g., as described in Terstappen et al. (Nat Rev Drug Discovery, https://doi.org/10.1038/s41573-021-00139-y (2021)), the contents of which are incorporated herein by reference in its entirety. In some embodiments, the AAV particle is administered to the subject intravenously. In some embodiments, the subject is a human.

Administration to the CNS

In some embodiments, an AAV particle described herein (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) may be delivered by direct injection into the brain. As a non-limiting example, the brain delivery may be by intrahippocampal administration. In some embodiments, an AAV particle of the present disclosure may be administered to a subject by intraparenchymal administration. In some embodiments, the intraparenchymal administration is to tissue of the central nervous system. In some embodiments, an AAV particle of the present disclosure may be administered to a subject by intracranial delivery (See, e.g., U.S. Pat. No. 8,119,611; the content of which is incorporated herein by reference in its entirety). In some embodiments, an AAV particle described herein may be delivered by injection into the CSF pathway. Non-limiting examples of delivery to the CSF pathway include intrathecal and intracerebroventricular administration.

In some embodiments, an AAV particle of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) may be delivered to the brain by systemic delivery. As a non-limiting example, the systemic delivery may be by intravascular administration. As a non-limiting example, the systemic or intravascular administration may be intravenous.

In some embodiments, an AAV particle of the present disclosure may be delivered by an intraocular delivery route. A non-limiting example of an intraocular administration includes an intravitreal injection.

Intramuscular Administration

In some embodiments, an AAV particle described herein (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) may be delivered by intramuscular administration. Without wishing to be bound by theory, it is believed in some embodiments, that the multi-nucleated nature of muscle cells provides an advantage to gene transduction subsequent to AAV delivery. In some embodiments, cells of the muscle are capable of expressing recombinant proteins with the appropriate post-translational modifications. Without wishing to be bound by theory, it is believed in some embodiments, the enrichment of muscle tissue with vascular structures allows for transfer to the blood stream and whole-body delivery. Examples of intramuscular administration include systemic (e.g., intravenous), subcutaneous or directly into the muscle. In some embodiments, more than one injection is administered. In some embodiments, an AAV particle of the present disclosure may be delivered by an intramuscular delivery route. (See, e.g., U.S. Pat. No. 6,506,379; the content of which is incorporated herein by reference in its entirety). Non-limiting examples of intramuscular administration include an intravenous injection or a subcutaneous injection.

In some embodiments, an AAV particle of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) is administered to a subject and transduces the muscle of a subject. As a non-limiting example, an AAV particle is administered by intramuscular administration. In some embodiments, an AAV particle of the present disclosure may be administered to a subject by subcutaneous administration. In some embodiments, the intramuscular administration is via systemic delivery. In some embodiments, the intramuscular administration is via intravenous delivery. In some embodiments, the intramuscular administration is via direct injection to the muscle.

In some embodiments, the muscle is transduced by administration, e.g., intramuscular administration. In some embodiments, an intramuscular delivery comprises administration at one site. In some embodiments, an intramuscular delivery comprises administration at more than one site. In some embodiments, an intramuscular delivery comprises administration at two, three, four, or more sites. In some embodiments, intramuscular delivery is combined with at least one other method of administration.

In some embodiments, an AAV particle pf the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) may be administered to a subject by peripheral injections. Non-limiting examples of peripheral injections include intraperitoneal, intramuscular, intravenous, conjunctival, or joint injection. It was disclosed in the art that the peripheral administration of AAV vectors can be transported to the central nervous system, for example, to the motor neurons (e.g., U.S. Patent Publication Nos. US20100240739 and US20100130594; the content of each of which is incorporated herein by reference in their entirety).

In some embodiments, an AAV particle of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) may be administered to a subject by intraparenchymal administration. In some embodiments, the intraparenchymal administration is to muscle tissue. In some embodiments, an AAV particle of the present disclosure is delivered as described in Bright et al 2015 (Neurobiol Aging. 36(2):693-709), the contents of which are herein incorporated by reference in their entirety. In some embodiments, an AAV particle of the present disclosure is administered to the gastrocnemius muscle of a subject. In some embodiments, an AAV particle of the present disclosure is administered to the bicep femorii of the subject. In some embodiments, an AAV particles of the present disclosure is administered to the tibialis anterior muscles. In some embodiments, an AAV particle of the present disclosure is administered to the soleus muscle.

Depot Administration

As described herein, in some embodiments, a pharmaceutical composition and/or an AAV particle of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) are formulated in depots for extended release. Generally, specific organs or tissues are targeted for administration.

In some embodiments, a pharmaceutical composition and/or an AAV particle of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) are spatially retained within or proximal to target tissues. Provided are methods of providing a pharmaceutical composition, an AAV particle, to target tissues of mammalian subjects by contacting target tissues (which comprise one or more target cells) with the pharmaceutical composition and/or the AAV particle, under conditions such that they are substantially retained in target tissues, e.g., such that at least 10, 20, 30, 40, 50, 60, 70, 80, 85, 90, 95, 96, 97, 98, 99, 99.9, 99.99 or greater than 99.99% of the composition is retained in the target tissues. In some embodiments, retention is determined by measuring the amount of pharmaceutical composition and/or AAV particle, that enter a target cell or a plurality of target cells. For example, at least 1%, 5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99.9%, 99.99%, or greater than 99.99% of a pharmaceutical composition and/or an AAV particle, administered to a subject are present intracellularly at a period of time following administration. For example, intramuscular injection to a subject may be performed using aqueous compositions comprising a pharmaceutical composition and/or an AAV particle of the present disclosure and a transfection reagent, and retention is determined by measuring the amount of the pharmaceutical composition and/or the AAV particle, present in the muscle cell or plurality of muscle cells.

In some embodiments, disclosed herein are methods of providing a pharmaceutical composition and/or an AAV particle of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) to a tissue of a subject, by contacting the tissue (comprising a cell, e.g., a plurality of cells) with the pharmaceutical composition and/or the AAV particle under conditions such that they are substantially retained in the tissue. In some embodiments, a pharmaceutical composition and/or AAV particle described herein comprise a sufficient amount of an active ingredient such that the effect of interest is produced in at least one cell. In some embodiments, a pharmaceutical composition and/or an AAV particle generally comprise one or more cell penetration agents. In some embodiments, the disclosure provides a naked formulations (such as without cell penetration agents or other agents), with or without pharmaceutically acceptable carriers.

Methods of Treatment

AAV Particles Encoding Protein Payloads

Provided in the present disclosure are methods for introducing the AAV particles of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) into cells, the method comprising introducing into said cells any of the vectors in an amount sufficient to modulate, e.g., increase, the production of a target mRNA and/or protein. In some aspects, the cells may be neurons such as but not limited to, motor, hippocampal, entorhinal, thalamic, cortical, sensory, sympathetic, or parasympathetic neurons, and glial cells such as astrocytes, microglia, and/or oligodendrocytes. In other aspects, the cells may be a muscle cell, e.g., a cell of a diaphragm, a quadriceps, or a heart (e.g., a heart atrium or a heart ventricle).

Disclosed in the present disclosure are methods for treating a neurological disease/disorder or a neurodegenerative disorder, a muscular or neuromuscular disorder, or a neurooncological disorder associated with aberrant, e.g., insufficient or increased, function/presence of a protein, e.g., a target protein in a subject in need of treatment. The method comprises administering to the subject a therapeutically effective amount of a composition comprising AAV particles of the present disclosure. As a non-limiting example, the AAV particles can increase target gene expression, increase target protein production, and thus reduce one or more symptoms of neurological disease in the subject such that the subject is therapeutically treated.

In some embodiments, the composition comprising the AAV particles of the present disclosure is administered to the central nervous system of the subject via systemic administration. In some embodiments, the systemic administration is intravenous (IV) injection. In some embodiments, the AAV particle described herein or a pharmaceutical composition comprising an AAV particle described herein is administered by focused ultrasound (FUS), e.g., coupled with the intravenous administration of microbubbles (FUS-MB) or MRI-guided FUS coupled with intravenous administration.

In some embodiments, the composition comprising the AAV particle of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) is administered to the central nervous system of the subject via intraventricular administration. In some embodiments, the intraventricular administration is intra-cisterna magna injection (ICM).

In some embodiments, the composition comprising an AAV particle of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) is administered to the central nervous system of the subject via intraventricular injection and intravenous injection.

In some embodiments, the composition comprising the AAV particle of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) is administered to the central nervous system of the subject via ICM injection and intravenous injection at a specific dose per subject. As a non-limiting example, the AAV particles are administered via ICM injection at a dose of 1×10⁴ VG per subject. As a non-limiting example, the AAV particles are administered via IV injection at a dose of 2×10¹³ VG per subject.

In some embodiments, the composition comprising the AAV particle of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) is administered to the central nervous system of the subject. In other embodiments, the composition comprising the AAV particles of the present disclosure is administered to a CNS tissue of a subject (e.g., putamen, thalamus or cortex of the subject).

In some embodiments, the composition comprising the AAV particle of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) is administered to the central nervous system of the subject via intraparenchymal injection. Non-limiting examples of intraparenchymal injections include intraputamenal, intracortical, intrathalamic, intrastriatal, intrahippocampal or into the entorhinal cortex.

In some embodiments, the composition comprising the AAV particle of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) is administered to the central nervous system of the subject via intraparenchymal injection and intravenous injection.

In some embodiments, the composition comprising the AAV particle of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) is administered to the central nervous system of the subject via intraventricular injection, intraparenchymal injection and intravenous injection.

In some embodiments, the composition comprising an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) of a plurality of particles of the present disclosure is administered to a muscle of the subject via intravenous injection. In some embodiments, the composition comprising an AAV particle of a plurality of particles of the present disclosure is administered to a muscle of the subject via intramuscular injection.

In some embodiments, an AAV particle of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) may be delivered into specific types of targeted cells, including, but not limited to, thalamic, hippocampal, entorhinal, cortical, motor, sensory, excitatory, inhibitory, sympathetic, or parasympathetic neurons; glial cells including oligodendrocytes, astrocytes and microglia; and/or other cells surrounding neurons such as T cells. In some embodiments, an AAV particle of the present disclosure may be delivered into a muscle cell, e.g., a cell of the quadriceps, diaphragm, liver, and/or heart.

In some embodiments, an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant), e.g., a plurality of particles, of the present disclosure may be delivered to neurons in the putamen, thalamus and/or cortex.

In some embodiments, an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant), e.g., a plurality of particles, of the present disclosure may be used as a therapy for neurological disease.

In some embodiments, an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant), e.g., a plurality of particles, of the present disclosure may be used as a therapy for tauopathies.

In some embodiments, an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant), e.g., a plurality of particles, of the present disclosure may be used as a therapy for Alzheimer's Disease.

In some embodiments, an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant), e.g., a plurality of particles, of the present disclosure may be used as a therapy for Amyotrophic Lateral Sclerosis.

In some embodiments, an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant), e.g., a plurality of particles, of the present disclosure may be used as a therapy for Huntington's Disease.

In some embodiments, an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant), e.g., a plurality of particles, of the present disclosure may be used as a therapy for Parkinson's Disease.

In some embodiments, an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant), e.g., a plurality of particles, of the present disclosure may be used as a therapy for Friedreich's Ataxia.

In some embodiments, an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant), e.g., a plurality of particles, of the present disclosure may be used as a therapy for chronic or neuropathic pain.

In some embodiments, an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant), e.g., a plurality of particles, of the present disclosure may be used as a therapy for a muscular disorder or a neuromuscular disorder.

In some embodiments, an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) e.g., a plurality of particles, of the present disclosure may be used as a therapy for a neuro-oncological disorder. In some embodiments, the neuro-oncological disorder is a cancer of primary CNS origin (e.g., a cancer of a CNS cell and/or CNS tissue). In some embodiments, the neuro-oncological disorder is metastatic cancer in a CNS cell, CNS region, and/or a CNS tissue.

In some embodiments, administration of the AAV particle described herein (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) to a subject may increase target protein levels in a subject. The target protein levels may be increased by about 30%, 40%, 50%, 60%, 70%, 80%, 85%, 90%, 95% and 100%, or at least 20-30%, 20-40%, 20-50%, 20-60%, 20-70%, 20-80%, 20-90%, 20-95%, 20-100%, 30-40%, 30-50%, 30-60%, 30-70%, 30-80%, 30-90%, 30-95%, 30-100%, 40-50%, 40-60%, 40-70%, 40-80%, 40-90%, 40-95%, 40-100%, 50-60%, 50-70%, 50-80%, 50-90%, 50-95%, 50-100%, 60-70%, 60-80%, 60-90%, 60-95%, 60-100%, 70-80%, 70-90%, 70-95%, 70-100%, 80-90%, 80-95%, 80-100%, 90-95%, 90-100% or 95-100% in a subject such as, but not limited to, the CNS, a region of the CNS, or a specific cell of the CNS, or a muscle, a region of a muscle, or a cell of a muscle, of a subject. As a non-limiting example, the AAV particles may increase the protein levels of a target protein by at least 50%. As a non-limiting example, the AAV particles may increase the proteins levels of a target protein by at least 40%. As a non-limiting example, a subject may have an increase of 10% of target protein. As a non-limiting example, the AAV particles may increase the protein levels of a target protein by fold increases over baseline. In some embodiments, AAV particles lead to 5-6 times higher levels of a target protein.

In some embodiments, administration of the AAV particle described herein (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) to a subject may increase the expression of a target protein in a subject. The expression of the target protein may be increased by about 30%, 40%, 50%, 60%, 70%, 80%, 85%, 90%, 95% and 100%, or at least 20-30%, 20-40%, 20-50%, 20-60%, 20-70%, 20-80%, 20-90%, 20-95%, 20-100%, 30-40%, 30-50%, 30-60%, 30-70%, 30-80%, 30-90%, 30-95%, 30-100%, 40-50%, 40-60%, 40-70%, 40-80%, 40-90%, 40-95%, 40-100%, 50-60%, 50-70%, 50-80%, 50-90%, 50-95%, 50-100%, 60-70%, 60-80%, 60-90%, 60-95%, 60-100%, 70-80%, 70-90%, 70-95%, 70-100%, 80-90%, 80-95%, 80-100%, 90-95%, 90-100% or 95-100% in a subject such as, but not limited to, the CNS, a region of the CNS, or a specific cell of the CNS or a muscle, a region of a muscle, or a cell of a muscle of a subject. As a non-limiting example, the AAV particles may increase the expression of a target protein by at least 50%. As a non-limiting example, the AAV particles may increase the expression of a target protein by at least 40%.

In some embodiments, intravenous administration of the AAV particles described herein (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) to a subject may increase the CNS expression or expression in a muscle, of a target protein in a subject. The expression of the target protein may be increased by about 30%, 40%, 50%, 60%, 70%, 80%, 85%, 90%, 95% and 100%, or at least 20-30%, 20-40%, 20-50%, 20-60%, 20-70%, 20-80%, 20-90%, 20-95%, 20-100%, 30-40%, 30-50%, 30-60%, 30-70%, 30-80%, 30-90%, 30-95%, 30-100%, 40-50%, 40-60%, 40-70%, 40-80%, 40-90%, 40-95%, 40-100%, 50-60%, 50-70%, 50-80%, 50-90%, 50-95%, 50-100%, 60-70%, 60-80%, 60-90%, 60-95%, 60-100%, 70-80%, 70-90%, 70-95%, 70-100%, 80-90%, 80-95%, 80-100%, 90-95%, 90-100% or 95-100% in a subject such as, but not limited to, the CNS, a region of the CNS, or a specific cell of the CNS or a muscle, a region of a muscle, or a cell of a muscle of a subject. As a non-limiting example, the AAV particles may increase the expression of a target protein in the CNS by at least 50%. As a non-limiting example, the AAV particles may increase the expression of a target protein in the CNS by at least 40%. In some embodiments, the AAV particle may increase expression of a target protein in a muscle by at least 50%. In some embodiments, the AAV particle may increase expression of a target protein in a muscle by at least 50%.

In some embodiments, the AAV particles of the present disclosure (e.g., an AAV particle comprising, an AAV capsid polypeptide, e.g., an AAV capsid variant) may be used to increase target protein expression in astrocytes in order to treat a neurological disease. Target protein in astrocytes may be increased by 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or more than 95%, 5-15%, 5-20%, 5-25%, 5-30%, 5-35%, 5-40%, 5-45%, 5-50%, 5-55%, 5-60%, 5-65%, 5-70%, 5-75%, 5-80%, 5-85%, 5-90%, 5-95%, 10-20%, 10-25%, 10-30%, 10-35%, 10-40%, 10-45%, 10-50%, 10-55%, 10-60%, 10-65%, 10-70%, 10-75%, 10-80%, 10-85%, 10-90%, 10-95%, 15-25%, 15-30%, 15-35%, 15-40%, 15-45%, 15-50%, 15-55%, 15-60%, 15-65%, 15-70%, 15-75%, 15-80%, 15-85%, 15-90%, 15-95%, 20-30%, 20-35%, 20-40%, 20-45%, 20-50%, 20-55%, 20-60%, 20-65%, 20-70%, 20-75%, 20-80%, 20-85%, 20-90%, 20-95%, 25-35%, 25-40%, 25-45%, 25-50%, 25-55%, 25-60%, 25-65%, 25-70%, 25-75%, 25-80%, 25-85%, 25-90%, 25-95%, 30-40%, 30-45%, 30-50%, 30-55%, 30-60%, 30-65%, 30-70%, 30-75%, 30-80%, 30-85%, 30-90%, 30-95%, 35-45%, 35-50%, 35-55%, 35-60%, 35-65%, 35-70%, 35-75%, 35-80%, 35-85%, 35-90%, 35-95%, 40-50%, 40-55%, 40-60%, 40-65%, 40-70%, 40-75%, 40-80%, 40-85%, 40-90%, 40-95%, 45-55%, 45-60%, 45-65%, 45-70%, 45-75%, 45-80%, 45-85%, 45-90%, 45-95%, 50-60%, 50-65%, 50-70%, 50-75%, 50-80%, 50-85%, 50-90%, 50-95%, 55-65%, 55-70%, 55-75%, 55-80%, 55-85%, 55-90%, 55-95%, 60-70%, 60-75%, 60-80%, 60-85%, 60-90%, 60-95%, 65-75%, 65-80%, 65-85%, 65-90%, 65-95%, 70-80%, 70-85%, 70-90%, 70-95%, 75-85%, 75-90%, 75-95%, 80-90%, 80-95%, or 90-95%.

In some embodiments, the AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) may be used to increase target protein in microglia. The increase of target protein in microglia may be, independently, increased by 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or more than 95%, 5-15%, 5-20%, 5-25%, 5-30%, 5-35%, 5-40%, 5-45%, 5-50%, 5-55%, 5-60%, 5-65%, 5- 70%, 5-75%, 5-80%, 5-85%, 5-90%, 5-95%, 10-20%, 10-25%, 10-30%, 10-35%, 10-40%, 10-45%, 10-50%, 10-55%, 10-60%, 10-65%, 10-70%, 10-75%, 10-80%, 10-85%, 10-90%, 10-95%, 15-25%, 15-30%, 15-35%, 15-40%, 15-45%, 15-50%, 15-55%, 15-60%, 15-65%, 15-70%, 15-75%, 15-80%, 15-85%, 15-90%, 15-95%, 20-30%, 20-35%, 20-40%, 20-45%, 20-50%, 20-55%, 20-60%, 20-65%, 20-70%, 20-75%, 20-80%, 20-85%, 20-90%, 20-95%, 25-35%, 25-40%, 25-45%, 25-50%, 25-55%, 25-60%, 25-65%, 25-70%, 25-75%, 25-80%, 25-85%, 25-90%, 25-95%, 30-40%, 30-45%, 30-50%, 30-55%, 30-60%, 30-65%, 30-70%, 30-75%, 30-80%, 30-85%, 30-90%, 30-95%, 35-45%, 35-50%, 35-55%, 35-60%, 35-65%, 35-70%, 35-75%, 35-80%, 35-85%, 35-90%, 35-95%, 40-50%, 40-55%, 40-60%, 40-65%, 40-70%, 40-75%, 40-80%, 40-85%, 40-90%, 40-95%, 45-55%, 45-60%, 45-65%, 45-70%, 45-75%, 45-80%, 45-85%, 45-90%, 45-95%, 50-60%, 50-65%, 50-70%, 50-75%, 50-80%, 50-85%, 50-90%, 50-95%, 55-65%, 55-70%, 55-75%, 55-80%, 55-85%, 55-90%, 55-95%, 60-70%, 60-75%, 60-80%, 60-85%, 60-90%, 60-95%, 65-75%, 65-80%, 65-85%, 65-90%, 65-95%, 70-80%, 70-85%, 70-90%, 70-95%, 75-85%, 75-90%, 75-95%, 80-90%, 80-95%, or 90-95%.

In some embodiments, the AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) may be used to increase target protein in cortical neurons. The increase of target protein in the cortical neurons may be, independently, increased by 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or more than 95%, 5-15%, 5-20%, 5-25%, 5-30%, 5-35%, 5-40%, 5-45%, 5-50%, 5-55%, 5-60%, 5-65%, 5-70%, 5-75%, 5-80%, 5-85%, 5-90%, 5-95%, 10-20%, 10-25%, 10-30%, 10-35%, 10-40%, 10-45%, 10-50%, 10-55%, 10-60%, 10-65%, 10-70%, 10-75%, 10-80%, 10-85%, 10-90%, 10-95%, 15-25%, 15-30%, 15-35%, 15-40%, 15-45%, 15-50%, 15-55%, 15-60%, 15-65%, 15-70%, 15-75%, 15-80%, 15-85%, 15-90%, 15-95%, 20-30%, 20-35%, 20-40%, 20-45%, 20-50%, 20-55%, 20-60%, 20-65%, 20-70%, 20-75%, 20-80%, 20-85%, 20-90%, 20-95%, 25-35%, 25-40%, 25-45%, 25-50%, 25-55%, 25-60%, 25-65%, 25-70%, 25-75%, 25-80%, 25-85%, 25-90%, 25-95%, 30-40%, 30-45%, 30-50%, 30-55%, 30-60%, 30-65%, 30-70%, 30-75%, 30-80%, 30-85%, 30-90%, 30-95%, 35-45%, 35-50%, 35-55%, 35-60%, 35-65%, 35-70%, 35-75%, 35-80%, 35-85%, 35-90%, 35-95%, 40-50%, 40-55%, 40-60%, 40-65%, 40-70%, 40-75%, 40-80%, 40-85%, 40-90%, 40-95%, 45-55%, 45-60%, 45-65%, 45-70%, 45-75%, 45-80%, 45-85%, 45-90%, 45-95%, 50-60%, 50-65%, 50-70%, 50-75%, 50-80%, 50-85%, 50-90%, 50-95%, 55-65%, 55-70%, 55-75%, 55-80%, 55-85%, 55-90%, 55-95%, 60-70%, 60-75%, 60-80%, 60-85%, 60-90%, 60-95%, 65-75%, 65-80%, 65-85%, 65-90%, 65-95%, 70-80%, 70-85%, 70-90%, 70-95%, 75-85%, 75-90%, 75-95%, 80-90%, 80-95%, or 90-95%.

In some embodiments, the AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) may be used to increase target protein in hippocampal neurons. The increase of target protein in the hippocampal neurons may be, independently, increased by 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or more than 95%, 5-15%, 5-20%, 5-25%, 5-30%, 5-35%, 5-40%, 5-45%, 5-50%, 5-55%, 5-60%, 5-65%, 5-70%, 5-75%, 5-80%, 5-85%, 5-90%, 5-95%, 10-20%, 10-25%, 10-30%, 10-35%, 10-40%, 10-45%, 10-50%, 10-55%, 10-60%, 10-65%, 10-70%, 10-75%, 10-80%, 10-85%, 10-90%, 10-95%, 15-25%, 15-30%, 15-35%, 15-40%, 15-45%, 15-50%, 15-55%, 15-60%, 15-65%, 15-70%, 15-75%, 15-80%, 15-85%, 15-90%, 15-95%, 20-30%, 20-35%, 20-40%, 20-45%, 20-50%, 20-55%, 20-60%, 20-65%, 20-70%, 20-75%, 20-80%, 20-85%, 20-90%, 20-95%, 25-35%, 25-40%, 25-45%, 25-50%, 25-55%, 25-60%, 25-65%, 25-70%, 25-75%, 25-80%, 25-85%, 25-90%, 25-95%, 30-40%, 30-45%, 30-50%, 30-55%, 30-60%, 30-65%, 30-70%, 30-75%, 30-80%, 30-85%, 30-90%, 30-95%, 35-45%, 35-50%, 35-55%, 35-60%, 35-65%, 35-70%, 35-75%, 35-80%, 35-85%, 35-90%, 35-95%, 40-50%, 40-55%, 40-60%, 40-65%, 40-70%, 40-75%, 40-80%, 40-85%, 40-90%, 40-95%, 45-55%, 45-60%, 45-65%, 45-70%, 45-75%, 45-80%, 45-85%, 45-90%, 45-95%, 50-60%, 50-65%, 50-70%, 50-75%, 50-80%, 50-85%, 50-90%, 50-95%, 55-65%, 55-70%, 55-75%, 55-80%, 55-85%, 55-90%, 55-95%, 60-70%, 60-75%, 60-80%, 60-85%, 60-90%, 60-95%, 65-75%, 65-80%, 65-85%, 65-90%, 65-95%, 70-80%, 70-85%, 70-90%, 70-95%, 75-85%, 75-90%, 75-95%, 80-90%, 80-95%, or 90-95%.

In some embodiments, the AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) may be used to increase target protein in DRG and/or sympathetic neurons. The increase of target protein in the DRG and/or sympathetic neurons may be, independently, increased by 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or more than 95%, 5-15%, 5-20%, 5-25%, 5-30%, 5-35%, 5-40%, 5-45%, 5-50%, 5-55%, 5-60%, 5-65%, 5-70%, 5-75%, 5-80%, 5-85%, 5-90%, 5-95%, 10-20%, 10-25%, 10-30%, 10-35%, 10-40%, 10-45%, 10-50%, 10-55%, 10-60%, 10-65%, 10-70%, 10-75%, 10-80%, 10-85%, 10-90%, 10-95%, 15-25%, 15-30%, 15-35%, 15-40%, 15-45%, 15-50%, 15-55%, 15-60%, 15-65%, 15-70%, 15-75%, 15-80%, 15-85%, 15-90%, 15-95%, 20-30%, 20-35%, 20-40%, 20-45%, 20-50%, 20-55%, 20-60%, 20-65%, 20-70%, 20-75%, 20-80%, 20-85%, 20-90%, 20-95%, 25-35%, 25-40%, 25-45%, 25-50%, 25-55%, 25-60%, 25-65%, 25-70%, 25-75%, 25-80%, 25-85%, 25-90%, 25-95%, 30-40%, 30-45%, 30-50%, 30-55%, 30-60%, 30-65%, 30-70%, 30-75%, 30-80%, 30-85%, 30-90%, 30-95%, 35-45%, 35-50%, 35-55%, 35-60%, 35-65%, 35-70%, 35-75%, 35-80%, 35-85%, 35-90%, 35-95%, 40-50%, 40-55%, 40-60%, 40-65%, 40-70%, 40-75%, 40-80%, 40-85%, 40-90%, 40-95%, 45-55%, 45-60%, 45-65%, 45-70%, 45-75%, 45-80%, 45-85%, 45-90%, 45-95%, 50-60%, 50-65%, 50-70%, 50-75%, 50-80%, 50-85%, 50-90%, 50-95%, 55-65%, 55-70%, 55-75%, 55-80%, 55-85%, 55-90%, 55-95%, 60-70%, 60-75%, 60-80%, 60-85%, 60-90%, 60-95%, 65-75%, 65-80%, 65-85%, 65-90%, 65-95%, 70-80%, 70-85%, 70-90%, 70-95%, 75-85%, 75-90%, 75-95%, 80-90%, 80-95%, or 90-95%.

In some embodiments, the AAV particles of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) may be used to increase target protein in sensory neurons in order to treat neurological disease. Target protein in sensory neurons may be increased by 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or more than 95%, 5-15%, 5-20%, 5-25%, 5-30%, 5-35%, 5-40%, 5-45%, 5-50%, 5-55%, 5-60%, 5-65%, 5-70%, 5-75%, 5-80%, 5-85%, 5-90%, 5-95%, 10-20%, 10-25%, 10-30%, 10-35%, 10-40%, 10-45%, 10-50%, 10-55%, 10-60%, 10-65%, 10-70%, 10-75%, 10-80%, 10-85%, 10-90%, 10-95%, 15-25%, 15-30%, 15-35%, 15-40%, 15-45%, 15-50%, 15-55%, 15-60%, 15-65%, 15-70%, 15-75%, 15-80%, 15-85%, 15-90%, 15-95%, 20-30%, 20-35%, 20-40%, 20-45%, 20-50%, 20-55%, 20-60%, 20-65%, 20-70%, 20-75%, 20-80%, 20-85%, 20-90%, 20-95%, 25-35%, 25-40%, 25-45%, 25-50%, 25-55%, 25-60%, 25-65%, 25-70%, 25-75%, 25-80%, 25-85%, 25-90%, 25-95%, 30-40%, 30-45%, 30-50%, 30-55%, 30-60%, 30-65%, 30-70%, 30-75%, 30-80%, 30-85%, 30-90%, 30-95%, 35-45%, 35-50%, 35-55%, 35-60%, 35-65%, 35-70%, 35-75%, 35-80%, 35-85%, 35-90%, 35-95%, 40-50%, 40-55%, 40-60%, 40-65%, 40-70%, 40-75%, 40-80%, 40-85%, 40-90%, 40-95%, 45-55%, 45-60%, 45-65%, 45-70%, 45-75%, 45-80%, 45-85%, 45-90%, 45-95%, 50-60%, 50-65%, 50-70%, 50-75%, 50-80%, 50-85%, 50-90%, 50-95%, 55-65%, 55-70%, 55-75%, 55-80%, 55-85%, 55-90%, 55-95%, 60-70%, 60-75%, 60-80%, 60-85%, 60-90%, 60-95%, 65-75%, 65-80%, 65-85%, 65-90%, 65-95%, 70-80%, 70-85%, 70-90%, 70-95%, 75-85%, 75-90%, 75-95%, 80-90%, 80-95%, or 90-95%.

In some embodiments, the AAV particles of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) may be used to increase target protein and reduce symptoms of neurological disease in a subject. The increase of target protein and/or the reduction of symptoms of neurological disease may be, independently, altered (increased for the production of target protein and reduced for the symptoms of neurological disease) by 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or more than 95%, 5-15%, 5-20%, 5-25%, 5-30%, 5-35%, 5-40%, 5-45%, 5-50%, 5-55%, 5-60%, 5-65%, 5-70%, 5-75%, 5-80%, 5-85%, 5-90%, 5-95%, 10-20%, 10-25%, 10-30%, 10-35%, 10-40%, 10-45%, 10-50%, 10-55%, 10-60%, 10-65%, 10-70%, 10-75%, 10-80%, 10-85%, 10-90%, 10-95%, 15-25%, 15-30%, 15-35%, 15-40%, 15-45%, 15-50%, 15-55%, 15-60%, 15-65%, 15-70%, 15-75%, 15-80%, 15-85%, 15-90%, 15-95%, 20-30%, 20-35%, 20-40%, 20-45%, 20-50%, 20-55%, 20-60%, 20-65%, 20-70%, 20-75%, 20-80%, 20-85%, 20-90%, 20-95%, 25-35%, 25-40%, 25-45%, 25-50%, 25-55%, 25-60%, 25-65%, 25-70%, 25-75%, 25-80%, 25-85%, 25-90%, 25-95%, 30-40%, 30-45%, 30-50%, 30-55%, 30-60%, 30-65%, 30-70%, 30-75%, 30-80%, 30-85%, 30-90%, 30-95%, 35-45%, 35-50%, 35-55%, 35-60%, 35-65%, 35-70%, 35-75%, 35-80%, 35-85%, 35-90%, 35-95%, 40-50%, 40-55%, 40-60%, 40-65%, 40-70%, 40-75%, 40-80%, 40-85%, 40-90%, 40-95%, 45-55%, 45-60%, 45-65%, 45-70%, 45-75%, 45-80%, 45-85%, 45-90%, 45-95%, 50-60%, 50-65%, 50-70%, 50-75%, 50-80%, 50-85%, 50-90%, 50-95%, 55-65%, 55-70%, 55-75%, 55-80%, 55-85%, 55-90%, 55-95%, 60-70%, 60-75%, 60-80%, 60-85%, 60-90%, 60-95%, 65-75%, 65-80%, 65-85%, 65-90%, 65-95%, 70-80%, 70-85%, 70-90%, 70-95%, 75-85%, 75-90%, 75-95%, 80-90%, 80-95%, or 90-95%.

In some embodiments, the AAV particles of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) may be used to reduce the decline of functional capacity and activities of daily living as measured by a standard evaluation system such as, but not limited to, the total functional capacity (TFC) scale.

In some embodiments, the AAV particles of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) may be used to improve performance on any assessment used to measure symptoms of neurological disease. Such assessments include, but are not limited to ADAS-cog (Alzheimer Disease Assessment Scale—cognitive), MMSE (Mini-Mental State Examination), GDS (Geriatric Depression Scale), FAQ (Functional Activities Questionnaire), ADL (Activities of Daily Living), GPCOG (General Practitioner Assessment of Cognition), Mini-Cog, AMTS (Abbreviated Mental Test Score), Clock-drawing test, 6-CIT (6-item Cognitive Impairment Test), TYM (Test Your Memory), MoCa (Montreal Cognitive Assessment), ACE-R (Addenbrookes Cognitive Assessment), MIS (Memory Impairment Screen), BADLS (Bristol Activities of Daily Living Scale), Barthel Index, Functional Independence Measure, Instrumental Activities of Daily Living, IQCODE (Informant Questionnaire on Cognitive Decline in the Elderly), Neuropsychiatric Inventory, The Cohen-Mansfield Agitation Inventory, BEHAVE-AD, EuroQol, Short Form-36 and/or MBR Caregiver Strain Instrument, or any of the other tests as described in Sheehan B (Ther Adv Neurol Disord. 5(6):349-358 (2012)), the contents of which are herein incorporated by reference in their entirety.

In some embodiments, the present composition is administered as a solo therapeutic or as combination therapeutic for the treatment of a neurological disease/disorder or a neurodegenerative disorder, a muscular disorder or neuromuscular disorder, and/or a neuro-oncological disorder.

The AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) encoding the target protein may be used in combination with one or more other therapeutic agents. In some embodiments, compositions can be administered concurrently with, prior to, or subsequent to, one or more other desired therapeutics or medical procedures. In general, each agent will be administered at a dose and/or on a time schedule determined for that agent.

Therapeutic agents that may be used in combination with the AAV particles of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) can be small molecule compounds which are antioxidants, anti-inflammatory agents, anti-apoptosis agents, calcium regulators, anti-glutamatergic agents, structural protein inhibitors, compounds involved in muscle function, and compounds involved in metal ion regulation. As a non-limiting example, the combination therapy may be in combination with one or more neuroprotective agents such as small molecule compounds, growth factors and hormones which have been tested for their neuroprotective effect on motor neuron degeneration.

Compounds tested for treating neurological disease which may be used in combination with the AAV particles described herein include, but are not limited to, cholinesterase inhibitors (donepezil, rivastigmine, galantamine), NMDA receptor antagonists such as memantine, anti-psychotics, anti-depressants, anti-convulsants (e.g., sodium valproate and levetiracetam for myoclonus), secretase inhibitors, amyloid aggregation inhibitors, copper or zinc modulators, BACE inhibitors, inhibitors of tau aggregation, such as Methylene blue, phenothiazines, anthraquinones, n-phenylamines or rhodamines, microtubule stabilizers such as NAP, taxol or paclitaxel, kinase or phosphatase inhibitors such as those targeting GSK3β (lithium) or PP2A, immunization with Aβ peptides or tau phospho-epitopes, anti-tau or anti-amyloid antibodies, dopamine-depleting agents (e.g., tetrabenazine for chorea), benzodiazepines (e.g., clonazepam for myoclonus, chorea, dystonia, rigidity, and/or spasticity), amino acid precursors of dopamine (e.g., levodopa for rigidity), skeletal muscle relaxants (e.g., baclofen, tizanidine for rigidity and/or spasticity), inhibitors for acetylcholine release at the neuromuscular junction to cause muscle paralysis (e.g., botulinum toxin for bruxism and/or dystonia), atypical neuroleptics (e.g., olanzapine and quetiapine for psychosis and/or irritability, risperidone, sulpiride and haloperidol for psychosis, chorea and/or irritability, clozapine for treatment-resistant psychosis, aripiprazole for psychosis with prominent negative symptoms), selective serotonin reuptake inhibitors (SSRIs) (e.g., citalopram, fluoxetine, paroxetine, sertraline, mirtazapine, venlafaxine for depression, anxiety, obsessive compulsive behavior and/or irritability), hypnotics (e.g., xopiclone and/or zolpidem for altered sleep-wake cycle), anticonvulsants (e.g., sodium valproate and carbamazepine for mania or hypomania) and mood stabilizers (e.g., lithium for mania or hypomania).

Neurotrophic factors may be used in combination therapy with the AAV particles of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) for treating neurological disease. Generally, a neurotrophic factor is defined as a substance that promotes survival, growth, differentiation, proliferation and/or maturation of a neuron, or stimulates increased activity of a neuron. In some embodiments, the present methods further comprise delivery of one or more trophic factors into the subject in need of treatment. Trophic factors may include, but are not limited to, IGF-I, GDNF, BDNF, CTNF, VEGF, Colivelin, Xaliproden, Thyrotrophin-releasing hormone and ADNF, and variants thereof.

In one aspect, the AAV particle described herein (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) may be co-administered with AAV particles expressing neurotrophic factors such as AAV-IGF-I (See e.g., Vincent et al., Neuromolecular medicine, 2004, 6, 79-85; the contents of which are incorporated herein by reference in their entirety) and AAV-GDNF (See e.g., Wang et al., J Neurosci., 2002, 22, 6920-6928; the contents of which are incorporated herein by reference in their entirety).

In some embodiments, administration of the AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) to a subject will modulate, e.g., increase or decrease, the expression of a target protein in a subject and the modulation, e.g., increase or decrease of the presence, level, activity, and/or expression of the target protein will reduce the effects and/or symptoms of a neurological disease/disorder or a neurodegenerative disorder, a muscular disorder or neuromuscular disorder, and/or a neuro-oncological disorder in a subject.

As a non-limiting example, the target protein may be a therapeutic protein or functional variant, or an antibody or antibody binding fragment thereof.

AAV Particles Comprising RNAi Agents or Modulatory Polynucleotides

Provided in the present disclosure are methods for introducing the AAV particles of the disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant), comprising a viral genome with a nucleic acid sequence encoding a payload comprising a siRNA molecule into cells, the method comprising introducing into said cells any of the vectors in an amount sufficient for degradation of a target mRNA to occur, thereby activating target-specific RNAi in the cells. In some aspects, the cells may be neurons such as but not limited to, motor, hippocampal, entorhinal, thalamic, cortical, sensory, sympathetic, or parasympathetic neurons, and glial cells such as astrocytes, microglia, and/or oligodendrocytes. In other aspects, the cells may be a muscle cell, e.g., a cell of a diaphragm, a quadriceps, or a heart (e.g., a heart atrium or a heart ventricle).

Disclosed in the present disclosure are methods for treating a neurological disease/disorder or a neurodegenerative disorder, a muscular or neuromuscular disorder, or a neurooncological disorder associated with dysfunction and/or aberrant, e.g., increased or decreased expression of a target protein in a subject in need of treatment. The method comprises administering to the subject a therapeutically effective amount of a composition comprising AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules. As a non-limiting example, the siRNA molecules can silence target gene expression, inhibit target protein production, and reduce one or more symptoms of neurological disease in the subject such that the subject is therapeutically treated.

In some embodiments, the composition comprising the AAV particles of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) comprising a viral genome encoding one or more siRNA molecules comprise an AAV capsid that allows for enhanced transduction of CNS and/or PNS cells after intravenous administration.

In some embodiments, the composition comprising the AAV particles of the present disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) with a viral genome encoding at least one siRNA molecule is administered to the central nervous system of the subject. In other embodiments, the composition comprising the AAV particles of the present disclosure is administered to a tissue of a subject (e.g., putamen, thalamus or cortex of the subject).

In some embodiments, the composition comprising the AAV particles of the disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant), comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules is administered to the central nervous system of the subject via systemic administration. In some embodiments, the systemic administration is intravenous injection. In some embodiments, the AAV particle described herein or a pharmaceutical composition comprising an AAV particle described herein is administered by focused ultrasound (FUS), e.g., coupled with the intravenous administration of microbubbles (FUS-MB) or MRI-guided FUS coupled with intravenous administration.

In some embodiments, the composition comprising the AAV particles of the disclosure (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules is administered to the central nervous system of the subject via intraparenchymal injection. Non-limiting examples of intraparenchymal injections include intraputamenal, intracortical, intrathalamic, intrastriatal, intrahippocampal or into the entorhinal cortex.

In some embodiments, the composition comprising the AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules is administered to the central nervous system of the subject via intraparenchymal injection and intravenous injection.

In some embodiments, the composition comprising an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) of a plurality of particles of the present disclosure is administered to a muscle of the subject via intravenous injection. In some embodiments, the composition comprising an AAV particle of a plurality of particles of the present disclosure is administered to a muscle of the subject via intramuscular injection.

In some embodiments, the AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules may be delivered into specific types or targeted cells, including, but not limited to, thalamic, hippocampal, entorhinal, cortical, motor, sensory, excitatory, inhibitory, sympathetic, or parasympathetic neurons; glial cells including oligodendrocytes, astrocytes and microglia; and/or other cells surrounding neurons such as T cells. In some embodiments, an AAV particle of the present disclosure may be delivered into a muscle cell, e.g., a cell of the quadriceps, diaphragm, liver, and/or heart.

In some embodiments, an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant), e.g., a plurality of particles, comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules may be delivered to neurons in the putamen, thalamus, and/or cortex.

In some embodiments, an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant), e.g., a plurality of particles, comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules may be used as a therapy for neurological disease.

In some embodiments, an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant), e.g., a plurality of particles, comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules may be used as a therapy for tauopathies.

In some embodiments, an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant), e.g., a plurality of particles, comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules may be used as a therapy for Alzheimer's Disease.

In some embodiments, an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant), e.g., a plurality of particles, comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules may be used as a therapy for Amyotrophic Lateral Sclerosis.

In some embodiments, an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant), e.g., a plurality of particles, comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules may be used as a therapy for Huntington's Disease.

In some embodiments, an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant), e.g., a plurality of particles, comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules may be used as a therapy for Parkinson's Disease.

In some embodiments, an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant), e.g., a plurality of particles, comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules may be used as a therapy for Friedreich's Ataxia.

In some embodiments, an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant), e.g., a plurality of particles, comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules may be used as a therapy for a muscular disorder or a neuromuscular disorder.

In some embodiments, an AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant), e.g., a plurality of particles, comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules may be used as a therapy for a neuro-oncological disorder. In some embodiments, the neuro-oncological disorder is a cancer of primary CNS origin (e.g., a cancer of a CNS cell and/or CNS tissue). In some embodiments, the neuro-oncological disorder is metastatic cancer in a CNS cell, a CNS region, and/or a CNS tissue.

In some embodiments, the administration of AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules to a subject may lower target protein levels in a subject. The target protein levels may be lowered by about 30%, 40%, 50%, 60%, 70%, 80%, 85%, 90%, 95% and 100%, or at least 20-30%, 20-40%, 20-50%, 20-60%, 20-70%, 20-80%, 20-90%, 20-95%, 20-100%, 30-40%, 30-50%, 30-60%, 30-70%, 30-80%, 30-90%, 30-95%, 30-100%, 40-50%, 40-60%, 40-70%, 40-80%, 40-90%, 40-95%, 40-100%, 50-60%, 50-70%, 50-80%, 50-90%, 50-95%, 50-100%, 60-70%, 60-80%, 60-90%, 60-95%, 60-100%, 70-80%, 70-90%, 70-95%, 70-100%, 80-90%, 80-95%, 80-100%, 90-95%, 90-100% or 95-100% in a subject such as, but not limited to, the CNS, a region of the CNS, or a specific cell of the CNS, or a muscle, a region of a muscle, or a cell of a muscle, of a subject. As a non-limiting example, the AAV particles may lower the protein levels of a target protein by at least 50%. As a non-limiting example, the AAV particles may lower the proteins levels of a target protein by at least 40%.

In some embodiments, the administration of AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules to a subject may lower the expression of a target protein in a subject. The expression of a target protein may be lowered by about 30%, 40%, 50%, 60%, 70%, 80%, 85%, 90%, 95% and 100%, or at least 20-30%, 20-40%, 20-50%, 20-60%, 20-70%, 20-80%, 20-90%, 20-95%, 20-100%, 30-40%, 30-50%, 30-60%, 30-70%, 30-80%, 30-90%, 30-95%, 30-100%, 40-50%, 40-60%, 40-70%, 40-80%, 40-90%, 40-95%, 40-100%, 50-60%, 50-70%, 50-80%, 50-90%, 50-95%, 50-100%, 60-70%, 60-80%, 60-90%, 60-95%, 60-100%, 70-80%, 70-90%, 70-95%, 70-100%, 80-90%, 80-95%, 80-100%, 90-95%, 90-100% or 95-100% in a subject such as, but not limited to, the CNS, a region of the CNS, or a specific cell of the CNS, or a muscle, a region of a muscle, or a cell of a muscle, of a subject. As a non-limiting example, the AAV particles may lower the expression of a target protein by at least 50%. As a non-limiting example, the AAV particles may lower the expression of a target protein by at least 40%.

In some embodiments, the administration of AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules to a subject may lower the expression of a target protein in the CNS of a subject. The expression of a target protein may be lowered by about 30%, 40%, 50%, 60%, 70%, 80%, 85%, 90%, 95% and 100%, or at least 20-30%, 20-40%, 20-50%, 20-60%, 20-70%, 20-80%, 20-90%, 20-95%, 20-100%, 30-40%, 30-50%, 30-60%, 30-70%, 30-80%, 30-90%, 30-95%, 30-100%, 40-50%, 40-60%, 40-70%, 40-80%, 40-90%, 40-95%, 40-100%, 50-60%, 50-70%, 50-80%, 50-90%, 50-95%, 50-100%, 60-70%, 60-80%, 60-90%, 60-95%, 60-100%, 70-80%, 70-90%, 70-95%, 70-100%, 80-90%, 80-95%, 80-100%, 90-95%, 90-100% or 95-100% in a subject such as, but not limited to, the CNS, a region of the CNS, or a specific cell of the CNS, or a muscle, a region of a muscle, or a cell of a muscle, of a subject. As a non-limiting example, the AAV particles may lower the expression of a target protein by at least 50%. As a non-limiting example, the AAV particles may lower the expression of a target protein by at least 40%.

In some embodiments, the AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules may be used to suppress a target protein in astrocytes in order to treat neurological disease. Target protein in astrocytes may be suppressed by 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or more than 95%, 5-15%, 5-20%, 5-25%, 5-30%, 5-35%, 5-40%, 5-45%, 5-50%, 5-55%, 5-60%, 5-65%, 5-70%, 5-75%, 5-80%, 5-85%, 5-90%, 5-95%, 10-20%, 10-25%, 10-30%, 10-35%, 10-40%, 10-45%, 10-50%, 10-55%, 10-60%, 10-65%, 10-70%, 10-75%, 10-80%, 10-85%, 10-90%, 10-95%, 15-25%, 15-30%, 15-35%, 15-40%, 15-45%, 15-50%, 15-55%, 15-60%, 15-65%, 15-70%, 15-75%, 15-80%, 15-85%, 15-90%, 15-95%, 20-30%, 20-35%, 20-40%, 20-45%, 20-50%, 20-55%, 20-60%, 20-65%, 20-70%, 20-75%, 20-80%, 20-85%, 20-90%, 20-95%, 25-35%, 25-40%, 25-45%, 25-50%, 25-55%, 25-60%, 25-65%, 25-70%, 25-75%, 25-80%, 25-85%, 25-90%, 25-95%, 30-40%, 30-45%, 30-50%, 30-55%, 30-60%, 30-65%, 30-70%, 30-75%, 30-80%, 30-85%, 30-90%, 30-95%, 35-45%, 35-50%, 35-55%, 35-60%, 35-65%, 35-70%, 35-75%, 35-80%, 35-85%, 35-90%, 35-95%, 40-50%, 40-55%, 40-60%, 40-65%, 40-70%, 40-75%, 40-80%, 40-85%, 40-90%, 40-95%, 45-55%, 45-60%, 45-65%, 45-70%, 45-75%, 45-80%, 45-85%, 45-90%, 45-95%, 50-60%, 50-65%, 50-70%, 50-75%, 50-80%, 50-85%, 50-90%, 50-95%, 55-65%, 55-70%, 55-75%, 55-80%, 55-85%, 55-90%, 55-95%, 60-70%, 60-75%, 60-80%, 60-85%, 60-90%, 60-95%, 65-75%, 65-80%, 65-85%, 65-90%, 65-95%, 70-80%, 70-85%, 70-90%, 70-95%, 75-85%, 75-90%, 75-95%, 80-90%, 80-95%, or 90-95%. Target protein in astrocytes may be reduced may be 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or more than 95%, 5-15%, 5-20%, 5-25%, 5-30%, 5-35%, 5-40%, 5-45%, 5-50%, 5-55%, 5-60%, 5-65%, 5-70%, 5-75%, 5-80%, 5-85%, 5-90%, 5-95%, 10-20%, 10-25%, 10-30%, 10-35%, 10-40%, 10-45%, 10-50%, 10-55%, 10-60%, 10-65%, 10-70%, 10-75%, 10-80%, 10-85%, 10-90%, 10-95%, 15-25%, 15-30%, 15-35%, 15-40%, 15-45%, 15-50%, 15-55%, 15-60%, 15-65%, 15-70%, 15-75%, 15-80%, 15-85%, 15-90%, 15-95%, 20-30%, 20-35%, 20-40%, 20-45%, 20-50%, 20-55%, 20-60%, 20-65%, 20-70%, 20-75%, 20-80%, 20-85%, 20-90%, 20-95%, 25-35%, 25-40%, 25-45%, 25-50%, 25-55%, 25-60%, 25-65%, 25-70%, 25-75%, 25-80%, 25-85%, 25-90%, 25-95%, 30-40%, 30-45%, 30-50%, 30-55%, 30-60%, 30-65%, 30-70%, 30-75%, 30-80%, 30-85%, 30-90%, 30-95%, 35-45%, 35-50%, 35-55%, 35-60%, 35-65%, 35-70%, 35-75%, 35-80%, 35-85%, 35-90%, 35-95%, 40-50%, 40-55%, 40-60%, 40-65%, 40-70%, 40-75%, 40-80%, 40-85%, 40-90%, 40-95%, 45-55%, 45-60%, 45-65%, 45-70%, 45-75%, 45-80%, 45-85%, 45-90%, 45-95%, 50-60%, 50-65%, 50-70%, 50-75%, 50-80%, 50-85%, 50-90%, 50-95%, 55-65%, 55-70%, 55-75%, 55-80%, 55-85%, 55-90%, 55-95%, 60-70%, 60-75%, 60-80%, 60-85%, 60-90%, 60-95%, 65-75%, 65-80%, 65-85%, 65-90%, 65-95%, 70-80%, 70-85%, 70-90%, 70-95%, 75-85%, 75-90%, 75-95%, 80-90%, 80-95%, or 90-95%.

In some embodiments, the AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules may be used to suppress a target protein in microglia. The suppression of the target protein in microglia may be, independently, suppressed by 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or more than 95%, 5-15%, 5-20%, 5-25%, 5-30%, 5-35%, 5-40%, 5-45%, 5-50%, 5-55%, 5-60%, 5-65%, 5-70%, 5-75%, 5-80%, 5-85%, 5-90%, 5-95%, 10-20%, 10-25%, 10-30%, 10-35%, 10-40%, 10-45%, 10-50%, 10-55%, 10-60%, 10-65%, 10-70%, 10-75%, 10-80%, 10-85%, 10-90%, 10-95%, 15-25%, 15-30%, 15-35%, 15-40%, 15-45%, 15-50%, 15-55%, 15-60%, 15-65%, 15-70%, 15-75%, 15-80%, 15-85%, 15-90%, 15-95%, 20-30%, 20-35%, 20-40%, 20-45%, 20-50%, 20-55%, 20-60%, 20-65%, 20-70%, 20-75%, 20-80%, 20-85%, 20-90%, 20-95%, 25-35%, 25-40%, 25-45%, 25-50%, 25-55%, 25-60%, 25-65%, 25-70%, 25-75%, 25-80%, 25-85%, 25-90%, 25-95%, 30-40%, 30-45%, 30-50%, 30-55%, 30-60%, 30-65%, 30-70%, 30-75%, 30-80%, 30-85%, 30-90%, 30-95%, 35-45%, 35-50%, 35-55%, 35-60%, 35-65%, 35-70%, 35-75%, 35-80%, 35-85%, 35-90%, 35-95%, 40-50%, 40-55%, 40-60%, 40-65%, 40-70%, 40-75%, 40-80%, 40-85%, 40-90%, 40-95%, 45-55%, 45-60%, 45-65%, 45-70%, 45-75%, 45-80%, 45-85%, 45-90%, 45-95%, 50-60%, 50-65%, 50-70%, 50-75%, 50-80%, 50-85%, 50-90%, 50-95%, 55-65%, 55-70%, 55-75%, 55-80%, 55-85%, 55-90%, 55-95%, 60-70%, 60-75%, 60-80%, 60-85%, 60-90%, 60-95%, 65-75%, 65-80%, 65-85%, 65-90%, 65-95%, 70-80%, 70-85%, 70-90%, 70-95%, 75-85%, 75-90%, 75-95%, 80-90%, 80-95%, or 90-95%. The reduction may be 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or more than 95%, 5-15%, 5-20%, 5-25%, 5-30%, 5-35%, 5-40%, 5-45%, 5-50%, 5-55%, 5-60%, 5-65%, 5-70%, 5-75%, 5-80%, 5-85%, 5-90%, 5-95%, 10-20%, 10-25%, 10-30%, 10-35%, 10-40%, 10-45%, 10-50%, 10-55%, 10-60%, 10-65%, 10-70%, 10-75%, 10-80%, 10-85%, 10-90%, 10-95%, 15-25%, 15-30%, 15-35%, 15-40%, 15-45%, 15-50%, 15-55%, 15-60%, 15-65%, 15-70%, 15-75%, 15-80%, 15-85%, 15-90%, 15-95%, 20-30%, 20-35%, 20-40%, 20-45%, 20-50%, 20-55%, 20-60%, 20-65%, 20-70%, 20-75%, 20-80%, 20-85%, 20-90%, 20-95%, 25-35%, 25-40%, 25-45%, 25-50%, 25-55%, 25-60%, 25-65%, 25-70%, 25-75%, 25-80%, 25-85%, 25-90%, 25-95%, 30-40%, 30-45%, 30-50%, 30-55%, 30-60%, 30-65%, 30-70%, 30-75%, 30-80%, 30-85%, 30-90%, 30-95%, 35-45%, 35-50%, 35-55%, 35-60%, 35-65%, 35-70%, 35-75%, 35-80%, 35-85%, 35-90%, 35-95%, 40-50%, 40-55%, 40-60%, 40-65%, 40-70%, 40-75%, 40-80%, 40-85%, 40-90%, 40-95%, 45-55%, 45-60%, 45-65%, 45-70%, 45-75%, 45-80%, 45-85%, 45-90%, 45-95%, 50-60%, 50-65%, 50-70%, 50-75%, 50-80%, 50-85%, 50-90%, 50-95%, 55-65%, 55-70%, 55-75%, 55-80%, 55-85%, 55-90%, 55-95%, 60-70%, 60-75%, 60-80%, 60-85%, 60-90%, 60-95%, 65-75%, 65-80%, 65-85%, 65-90%, 65-95%, 70-80%, 70-85%, 70-90%, 70-95%, 75-85%, 75-90%, 75-95%, 80-90%, 80-95%, or 90-95%.

In some embodiments, the AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules may be used to suppress target protein in cortical neurons. The suppression of a target protein in cortical neurons may be, independently, suppressed by 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or more than 95%, 5-15%, 5-20%, 5-25%, 5-30%, 5-35%, 5-40%, 5-45%, 5-50%, 5-55%, 5-60%, 5-65%, 5-70%, 5-75%, 5-80%, 5-85%, 5-90%, 5-95%, 10-20%, 10-25%, 10-30%, 10-35%, 10-40%, 10-45%, 10-50%, 10-55%, 10-60%, 10-65%, 10-70%, 10-75%, 10-80%, 10-85%, 10-90%, 10-95%, 15-25%, 15-30%, 15-35%, 15-40%, 15-45%, 15-50%, 15-55%, 15-60%, 15-65%, 15-70%, 15-75%, 15-80%, 15-85%, 15-90%, 15-95%, 20-30%, 20-35%, 20-40%, 20-45%, 20-50%, 20-55%, 20-60%, 20-65%, 20-70%, 20-75%, 20-80%, 20-85%, 20-90%, 20-95%, 25-35%, 25-40%, 25-45%, 25-50%, 25-55%, 25-60%, 25-65%, 25-70%, 25-75%, 25-80%, 25-85%, 25-90%, 25-95%, 30-40%, 30-45%, 30-50%, 30-55%, 30-60%, 30-65%, 30-70%, 30-75%, 30-80%, 30-85%, 30-90%, 30-95%, 35-45%, 35-50%, 35-55%, 35-60%, 35-65%, 35-70%, 35-75%, 35-80%, 35-85%, 35-90%, 35-95%, 40-50%, 40-55%, 40-60%, 40-65%, 40-70%, 40-75%, 40-80%, 40-85%, 40-90%, 40-95%, 45-55%, 45-60%, 45-65%, 45-70%, 45-75%, 45-80%, 45-85%, 45-90%, 45-95%, 50-60%, 50-65%, 50-70%, 50-75%, 50-80%, 50-85%, 50-90%, 50-95%, 55-65%, 55-70%, 55-75%, 55-80%, 55-85%, 55-90%, 55-95%, 60-70%, 60-75%, 60-80%, 60-85%, 60-90%, 60-95%, 65-75%, 65-80%, 65-85%, 65-90%, 65-95%, 70-80%, 70-85%, 70-90%, 70-95%, 75-85%, 75-90%, 75-95%, 80-90%, 80-95%, or 90-95%. The reduction may be 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or more than 95%, 5-15%, 5-20%, 5-25%, 5-30%, 5-35%, 5-40%, 5-45%, 5-50%, 5-55%, 5-60%, 5-65%, 5-70%, 5-75%, 5-80%, 5-85%, 5-90%, 5-95%, 10-20%, 10-25%, 10-30%, 10-35%, 10-40%, 10-45%, 10-50%, 10-55%, 10-60%, 10-65%, 10-70%, 10-75%, 10-80%, 10-85%, 10-90%, 10-95%, 15-25%, 15-30%, 15-35%, 15-40%, 15-45%, 15-50%, 15-55%, 15-60%, 15-65%, 15-70%, 15-75%, 15-80%, 15-85%, 15-90%, 15-95%, 20-30%, 20-35%, 20-40%, 20-45%, 20-50%, 20-55%, 20-60%, 20-65%, 20-70%, 20-75%, 20-80%, 20-85%, 20-90%, 20-95%, 25-35%, 25-40%, 25-45%, 25-50%, 25-55%, 25-60%, 25-65%, 25-70%, 25-75%, 25-80%, 25-85%, 25-90%, 25-95%, 30-40%, 30-45%, 30-50%, 30-55%, 30-60%, 30-65%, 30-70%, 30-75%, 30-80%, 30-85%, 30-90%, 30-95%, 35-45%, 35-50%, 35-55%, 35-60%, 35-65%, 35-70%, 35-75%, 35-80%, 35-85%, 35-90%, 35-95%, 40-50%, 40-55%, 40-60%, 40-65%, 40-70%, 40-75%, 40-80%, 40-85%, 40-90%, 40-95%, 45-55%, 45-60%, 45-65%, 45-70%, 45-75%, 45-80%, 45-85%, 45-90%, 45-95%, 50-60%, 50-65%, 50-70%, 50-75%, 50-80%, 50-85%, 50-90%, 50-95%, 55-65%, 55-70%, 55-75%, 55-80%, 55-85%, 55-90%, 55-95%, 60-70%, 60-75%, 60-80%, 60-85%, 60-90%, 60-95%, 65-75%, 65-80%, 65-85%, 65-90%, 65-95%, 70-80%, 70-85%, 70-90%, 70-95%, 75-85%, 75-90%, 75-95%, 80-90%, 80-95%, or 90-95%.

In some embodiments, the AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules may be used to suppress a target protein in hippocampal neurons. The suppression of a target protein in the hippocampal neurons may be, independently, suppressed by 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or more than 95%, 5-15%, 5-20%, 5-25%, 5-30%, 5-35%, 5-40%, 5-45%, 5-50%, 5-55%, 5-60%, 5-65%, 5-70%, 5-75%, 5-80%, 5-85%, 5-90%, 5-95%, 10-20%, 10-25%, 10-30%, 10-35%, 10-40%, 10-45%, 10-50%, 10-55%, 10-60%, 10-65%, 10-70%, 10-75%, 10-80%, 10-85%, 10-90%, 10-95%, 15-25%, 15-30%, 15-35%, 15-40%, 15-45%, 15-50%, 15-55%, 15-60%, 15-65%, 15-70%, 15-75%, 15-80%, 15-85%, 15-90%, 15-95%, 20-30%, 20-35%, 20-40%, 20-45%, 20-50%, 20-55%, 20-60%, 20-65%, 20-70%, 20-75%, 20-80%, 20-85%, 20-90%, 20-95%, 25-35%, 25-40%, 25-45%, 25-50%, 25-55%, 25-60%, 25-65%, 25-70%, 25-75%, 25-80%, 25-85%, 25-90%, 25-95%, 30-40%, 30-45%, 30-50%, 30-55%, 30-60%, 30-65%, 30-70%, 30-75%, 30-80%, 30-85%, 30-90%, 30-95%, 35-45%, 35-50%, 35-55%, 35-60%, 35-65%, 35-70%, 35-75%, 35-80%, 35-85%, 35-90%, 35-95%, 40-50%, 40-55%, 40-60%, 40-65%, 40-70%, 40-75%, 40-80%, 40-85%, 40-90%, 40-95%, 45-55%, 45-60%, 45-65%, 45-70%, 45-75%, 45-80%, 45-85%, 45-90%, 45-95%, 50-60%, 50-65%, 50-70%, 50-75%, 50-80%, 50-85%, 50-90%, 50-95%, 55-65%, 55-70%, 55-75%, 55-80%, 55-85%, 55-90%, 55-95%, 60-70%, 60-75%, 60-80%, 60-85%, 60-90%, 60-95%, 65-75%, 65-80%, 65-85%, 65-90%, 65-95%, 70-80%, 70-85%, 70-90%, 70-95%, 75-85%, 75-90%, 75-95%, 80-90%, 80-95%, or 90-95%. The reduction may be 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or more than 95%, 5-15%, 5-20%, 5-25%, 5-30%, 5-35%, 5-40%, 5-45%, 5-50%, 5-55%, 5-60%, 5-65%, 5-70%, 5-75%, 5-80%, 5-85%, 5-90%, 5-95%, 10-20%, 10-25%, 10-30%, 10-35%, 10-40%, 10-45%, 10-50%, 10-55%, 10-60%, 10-65%, 10-70%, 10-75%, 10-80%, 10-85%, 10-90%, 10-95%, 15-25%, 15-30%, 15-35%, 15-40%, 15-45%, 15-50%, 15-55%, 15-60%, 15-65%, 15-70%, 15-75%, 15-80%, 15-85%, 15-90%, 15-95%, 20-30%, 20-35%, 20-40%, 20-45%, 20-50%, 20-55%, 20-60%, 20-65%, 20-70%, 20-75%, 20-80%, 20-85%, 20-90%, 20-95%, 25-35%, 25-40%, 25-45%, 25-50%, 25-55%, 25-60%, 25-65%, 25-70%, 25-75%, 25-80%, 25-85%, 25-90%, 25-95%, 30-40%, 30-45%, 30-50%, 30-55%, 30-60%, 30-65%, 30-70%, 30-75%, 30-80%, 30-85%, 30-90%, 30-95%, 35-45%, 35-50%, 35-55%, 35-60%, 35-65%, 35-70%, 35-75%, 35-80%, 35-85%, 35-90%, 35-95%, 40-50%, 40-55%, 40-60%, 40-65%, 40-70%, 40-75%, 40-80%, 40-85%, 40-90%, 40-95%, 45-55%, 45-60%, 45-65%, 45-70%, 45-75%, 45-80%, 45-85%, 45-90%, 45-95%, 50-60%, 50-65%, 50-70%, 50-75%, 50-80%, 50-85%, 50-90%, 50-95%, 55-65%, 55-70%, 55-75%, 55-80%, 55-85%, 55-90%, 55-95%, 60-70%, 60-75%, 60-80%, 60-85%, 60-90%, 60-95%, 65-75%, 65-80%, 65-85%, 65-90%, 65-95%, 70-80%, 70-85%, 70-90%, 70-95%, 75-85%, 75-90%, 75-95%, 80-90%, 80-95%, or 90-95%.

In some embodiments, the AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules may be used to suppress a target protein in DRG and/or sympathetic neurons. The suppression of a target protein in the DRG and/or sympathetic neurons may be, independently, suppressed by 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or more than 95%, 5-15%, 5-20%, 5-25%, 5-30%, 5-35%, 5-40%, 5-45%, 5-50%, 5-55%, 5-60%, 5-65%, 5-70%, 5-75%, 5-80%, 5-85%, 5-90%, 5-95%, 10-20%, 10-25%, 10-30%, 10-35%, 10-40%, 10-45%, 10-50%, 10-55%, 10-60%, 10-65%, 10-70%, 10-75%, 10-80%, 10-85%, 10-90%, 10-95%, 15-25%, 15-30%, 15-35%, 15-40%, 15-45%, 15-50%, 15-55%, 15-60%, 15-65%, 15-70%, 15-75%, 15-80%, 15-85%, 15-90%, 15-95%, 20-30%, 20-35%, 20-40%, 20-45%, 20-50%, 20-55%, 20-60%, 20-65%, 20-70%, 20-75%, 20-80%, 20-85%, 20-90%, 20-95%, 25-35%, 25-40%, 25-45%, 25-50%, 25-55%, 25-60%, 25-65%, 25-70%, 25-75%, 25-80%, 25-85%, 25-90%, 25-95%, 30-40%, 30-45%, 30-50%, 30-55%, 30-60%, 30-65%, 30-70%, 30-75%, 30-80%, 30-85%, 30-90%, 30-95%, 35-45%, 35-50%, 35-55%, 35-60%, 35-65%, 35-70%, 35-75%, 35-80%, 35-85%, 35-90%, 35-95%, 40-50%, 40-55%, 40-60%, 40-65%, 40-70%, 40-75%, 40-80%, 40-85%, 40-90%, 40-95%, 45-55%, 45-60%, 45-65%, 45-70%, 45-75%, 45-80%, 45-85%, 45-90%, 45-95%, 50-60%, 50-65%, 50-70%, 50-75%, 50-80%, 50-85%, 50-90%, 50-95%, 55-65%, 55-70%, 55-75%, 55-80%, 55-85%, 55-90%, 55-95%, 60-70%, 60-75%, 60-80%, 60-85%, 60-90%, 60-95%, 65-75%, 65-80%, 65-85%, 65-90%, 65-95%, 70-80%, 70-85%, 70-90%, 70-95%, 75-85%, 75-90%, 75-95%, 80-90%, 80-95%, or 90-95%. The reduction may be 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or more than 95%, 5-15%, 5-20%, 5-25%, 5-30%, 5-35%, 5-40%, 5-45%, 5-50%, 5-55%, 5-60%, 5-65%, 5-70%, 5-75%, 5-80%, 5-85%, 5-90%, 5-95%, 10-20%, 10-25%, 10-30%, 10-35%, 10-40%, 10-45%, 10-50%, 10-55%, 10-60%, 10-65%, 10-70%, 10-75%, 10-80%, 10-85%, 10-90%, 10-95%, 15-25%, 15-30%, 15-35%, 15-40%, 15-45%, 15-50%, 15-55%, 15-60%, 15-65%, 15-70%, 15-75%, 15-80%, 15-85%, 15-90%, 15-95%, 20-30%, 20-35%, 20-40%, 20-45%, 20-50%, 20-55%, 20-60%, 20-65%, 20-70%, 20-75%, 20-80%, 20-85%, 20-90%, 20-95%, 25-35%, 25-40%, 25-45%, 25-50%, 25-55%, 25-60%, 25-65%, 25-70%, 25-75%, 25-80%, 25-85%, 25-90%, 25-95%, 30-40%, 30-45%, 30-50%, 30-55%, 30-60%, 30-65%, 30-70%, 30-75%, 30-80%, 30-85%, 30-90%, 30-95%, 35-45%, 35-50%, 35-55%, 35-60%, 35-65%, 35-70%, 35-75%, 35-80%, 35-85%, 35-90%, 35-95%, 40-50%, 40-55%, 40-60%, 40-65%, 40-70%, 40-75%, 40-80%, 40-85%, 40-90%, 40-95%, 45-55%, 45-60%, 45-65%, 45-70%, 45-75%, 45-80%, 45-85%, 45-90%, 45-95%, 50-60%, 50-65%, 50-70%, 50-75%, 50-80%, 50-85%, 50-90%, 50-95%, 55-65%, 55-70%, 55-75%, 55-80%, 55-85%, 55-90%, 55-95%, 60-70%, 60-75%, 60-80%, 60-85%, 60-90%, 60-95%, 65-75%, 65-80%, 65-85%, 65-90%, 65-95%, 70-80%, 70-85%, 70-90%, 70-95%, 75-85%, 75-90%, 75-95%, 80-90%, 80-95%, or 90-95%.

In some embodiments, the AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules may be used to suppress a target protein in sensory neurons in order to treat neurological disease. Target protein in sensory neurons may be suppressed by 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or more than 95%, 5-15%, 5-20%, 5-25%, 5-30%, 5-35%, 5-40%, 5-45%, 5-50%, 5-55%, 5-60%, 5-65%, 5-70%, 5-75%, 5-80%, 5-85%, 5-90%, 5-95%, 10-20%, 10-25%, 10-30%, 10-35%, 10-40%, 10-45%, 10-50%, 10-55%, 10-60%, 10-65%, 10-70%, 10-75%, 10-80%, 10-85%, 10-90%, 10-95%, 15-25%, 15-30%, 15-35%, 15-40%, 15-45%, 15-50%, 15-55%, 15-60%, 15-65%, 15-70%, 15-75%, 15-80%, 15-85%, 15-90%, 15-95%, 20-30%, 20-35%, 20-40%, 20-45%, 20-50%, 20-55%, 20-60%, 20-65%, 20-70%, 20-75%, 20-80%, 20-85%, 20-90%, 20-95%, 25-35%, 25-40%, 25-45%, 25-50%, 25-55%, 25-60%, 25-65%, 25-70%, 25-75%, 25-80%, 25-85%, 25-90%, 25-95%, 30-40%, 30-45%, 30-50%, 30-55%, 30-60%, 30-65%, 30-70%, 30-75%, 30-80%, 30-85%, 30-90%, 30-95%, 35-45%, 35-50%, 35-55%, 35-60%, 35-65%, 35-70%, 35-75%, 35-80%, 35-85%, 35-90%, 35-95%, 40-50%, 40-55%, 40-60%, 40-65%, 40-70%, 40-75%, 40-80%, 40-85%, 40-90%, 40-95%, 45-55%, 45-60%, 45-65%, 45-70%, 45-75%, 45-80%, 45-85%, 45-90%, 45-95%, 50-60%, 50-65%, 50-70%, 50-75%, 50-80%, 50-85%, 50-90%, 50-95%, 55-65%, 55-70%, 55-75%, 55-80%, 55-85%, 55-90%, 55-95%, 60-70%, 60-75%, 60-80%, 60-85%, 60-90%, 60-95%, 65-75%, 65-80%, 65-85%, 65-90%, 65-95%, 70-80%, 70-85%, 70-90%, 70-95%, 75-85%, 75-90%, 75-95%, 80-90%, 80-95%, or 90-95%. Target protein in the sensory neurons may be reduced may be 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or more than 95%, 5-15%, 5-20%, 5-25%, 5-30%, 5-35%, 5-40%, 5-45%, 5-50%, 5-55%, 5-60%, 5-65%, 5-70%, 5-75%, 5-80%, 5-85%, 5-90%, 5-95%, 10-20%, 10-25%, 10-30%, 10-35%, 10-40%, 10-45%, 10-50%, 10-55%, 10-60%, 10-65%, 10-70%, 10-75%, 10-80%, 10-85%, 10-90%, 10-95%, 15-25%, 15-30%, 15-35%, 15-40%, 15-45%, 15-50%, 15-55%, 15-60%, 15-65%, 15-70%, 15-75%, 15-80%, 15-85%, 15-90%, 15-95%, 20-30%, 20-35%, 20-40%, 20-45%, 20-50%, 20-55%, 20-60%, 20-65%, 20-70%, 20-75%, 20-80%, 20-85%, 20-90%, 20-95%, 25-35%, 25-40%, 25-45%, 25-50%, 25-55%, 25-60%, 25-65%, 25-70%, 25-75%, 25-80%, 25-85%, 25-90%, 25-95%, 30-40%, 30-45%, 30-50%, 30-55%, 30-60%, 30-65%, 30-70%, 30-75%, 30-80%, 30-85%, 30-90%, 30-95%, 35-45%, 35-50%, 35-55%, 35-60%, 35-65%, 35-70%, 35-75%, 35-80%, 35-85%, 35-90%, 35-95%, 40-50%, 40-55%, 40-60%, 40-65%, 40-70%, 40-75%, 40-80%, 40-85%, 40-90%, 40-95%, 45-55%, 45-60%, 45-65%, 45-70%, 45-75%, 45-80%, 45-85%, 45-90%, 45-95%, 50-60%, 50-65%, 50-70%, 50-75%, 50-80%, 50-85%, 50-90%, 50-95%, 55-65%, 55-70%, 55-75%, 55-80%, 55-85%, 55-90%, 55-95%, 60-70%, 60-75%, 60-80%, 60-85%, 60-90%, 60-95%, 65-75%, 65-80%, 65-85%, 65-90%, 65-95%, 70-80%, 70-85%, 70-90%, 70-95%, 75-85%, 75-90%, 75-95%, 80-90%, 80-95%, or 90-95%.

In some embodiments, the AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules may be used to suppress a target protein and reduce symptoms of neurological disease in a subject. The suppression of target protein and/or the reduction of symptoms of neurological disease may be, independently, reduced or suppressed by 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or more than 95%, 5-15%, 5-20%, 5-25%, 5-30%, 5-35%, 5-40%, 5-45%, 5-50%, 5-55%, 5-60%, 5-65%, 5-70%, 5-75%, 5-80%, 5-85%, 5-90%, 5-95%, 10-20%, 10-25%, 10-30%, 10-35%, 10-40%, 10-45%, 10-50%, 10-55%, 10-60%, 10-65%, 10-70%, 10-75%, 10-80%, 10-85%, 10-90%, 10-95%, 15-25%, 15-30%, 15-35%, 15-40%, 15-45%, 15-50%, 15-55%, 15-60%, 15-65%, 15-70%, 15-75%, 15-80%, 15-85%, 15-90%, 15-95%, 20-30%, 20-35%, 20-40%, 20-45%, 20-50%, 20-55%, 20-60%, 20-65%, 20-70%, 20-75%, 20-80%, 20-85%, 20-90%, 20-95%, 25-35%, 25-40%, 25-45%, 25-50%, 25-55%, 25-60%, 25-65%, 25-70%, 25-75%, 25-80%, 25-85%, 25-90%, 25-95%, 30-40%, 30-45%, 30-50%, 30-55%, 30-60%, 30-65%, 30-70%, 30-75%, 30-80%, 30-85%, 30-90%, 30-95%, 35-45%, 35-50%, 35-55%, 35-60%, 35-65%, 35-70%, 35-75%, 35-80%, 35-85%, 35-90%, 35-95%, 40-50%, 40-55%, 40-60%, 40-65%, 40-70%, 40-75%, 40-80%, 40-85%, 40-90%, 40-95%, 45-55%, 45-60%, 45-65%, 45-70%, 45-75%, 45-80%, 45-85%, 45-90%, 45-95%, 50-60%, 50-65%, 50-70%, 50-75%, 50-80%, 50-85%, 50-90%, 50-95%, 55-65%, 55-70%, 55-75%, 55-80%, 55-85%, 55-90%, 55-95%, 60-70%, 60-75%, 60-80%, 60-85%, 60-90%, 60-95%, 65-75%, 65-80%, 65-85%, 65-90%, 65-95%, 70-80%, 70-85%, 70-90%, 70-95%, 75-85%, 75-90%, 75-95%, 80-90%, 80-95%, or 90-95%.

In some embodiments, the AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules may be used to reduce the decline of functional capacity and activities of daily living as measured by a standard evaluation system such as, but not limited to, the total functional capacity (TFC) scale.

In some embodiments, the present composition is administered as a solo therapeutic or as combination therapeutic for the treatment of a neurological disease/disorder or a neurodegenerative disorder, a muscular disorder or neuromuscular disorder, and/or a neuro-oncological disorder.

The AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules may be used in combination with one or more other therapeutic agents. In some embodiments, compositions can be administered concurrently with, prior to, or subsequent to, one or more other desired therapeutics or medical procedures. In general, each agent will be administered at a dose and/or on a time schedule determined for that agent.

Therapeutic agents that may be used in combination with the AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules can be small molecule compounds which are antioxidants, anti-inflammatory agents, anti-apoptosis agents, calcium regulators, antiglutamatergic agents, structural protein inhibitors, compounds involved in muscle function, and compounds involved in metal ion regulation.

Compounds tested for treating neurological disease which may be used in combination with the AAV particles comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules include, but are not limited to, cholinesterase inhibitors (donepezil, rivastigmine, galantamine), NMDA receptor antagonists such as memantine, anti-psychotics, anti-depressants, anti-convulsants (e.g., sodium valproate and levetiracetam for myoclonus), secretase inhibitors, amyloid aggregation inhibitors, copper or zinc modulators, BACE inhibitors, inhibitors of tau aggregation, such as Methylene blue, phenothiazines, anthraquinones, n-phenylamines or rhodamines, microtubule stabilizers such as NAP, taxol or paclitaxel, kinase or phosphatase inhibitors such as those targeting GSK3β (lithium) or PP2A, immunization with Aβ peptides or tau phospho-epitopes, anti-tau or anti-amyloid antibodies, dopamine-depleting agents (e.g., tetrabenazine for chorea), benzodiazepines (e.g., clonazepam for myoclonus, chorea, dystonia, rigidity, and/or spasticity), amino acid precursors of dopamine (e.g., levodopa for rigidity), skeletal muscle relaxants (e.g., baclofen, tizanidine for rigidity and/or spasticity), inhibitors for acetylcholine release at the neuromuscular junction to cause muscle paralysis (e.g., botulinum toxin for bruxism and/or dystonia), atypical neuroleptics (e.g., olanzapine and quetiapine for psychosis and/or irritability, risperidone, sulpiride and haloperidol for psychosis, chorea and/or irritability, clozapine for treatment-resistant psychosis, aripiprazole for psychosis with prominent negative symptoms), selective serotonin reuptake inhibitors (SSRIs) (e.g., citalopram, fluoxetine, paroxetine, sertraline, mirtazapine, venlafaxine for depression, anxiety, obsessive compulsive behavior and/or irritability), hypnotics (e.g., xopiclone and/or zolpidem for altered sleep-wake cycle), anticonvulsants (e.g., sodium valproate and carbamazepine for mania or hypomania) and mood stabilizers (e.g., lithium for mania or hypomania).

Neurotrophic factors may be used in combination therapy with the AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) comprising a viral genome with a nucleic acid sequence encoding one or more siRNA molecules for treating neurological disease. Generally, a neurotrophic factor is defined as a substance that promotes survival, growth, differentiation, proliferation and/or maturation of a neuron, or stimulates increased activity of a neuron. In some embodiments, the present methods further comprise delivery of one or more trophic factors into the subject in need of treatment. Trophic factors may include, but are not limited to, IGF-I, GDNF, BDNF, CTNF, VEGF, Colivelin, Xaliproden, Thyrotrophin-releasing hormone and ADNF, and variants thereof.

In one aspect, the AAV particle (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) encoding the nucleic acid sequence for the at least one siRNA duplex targeting the gene of interest may be co-administered with AAV particles expressing neurotrophic factors such as AAV-IGF-I (See e.g., Vincent et al., Neuromolecular medicine, 2004, 6, 79-85; the content of which is incorporated herein by reference in its entirety) and AAV-GDNF (See e.g., Wang et al., J Neurosci., 2002, 22, 6920-6928; the contents of which are incorporated herein by reference in their entirety).

In some embodiments, administration of the AAV particles (e.g., an AAV particle comprising an AAV capsid polypeptide, e.g., an AAV capsid variant) to a subject will modulate, e.g., reduce the presence, level, activity, and/or expression of a target gene, mRNA, and/or protein in a subject and the reduction of expression of the target protein will reduce the effects and/or symptoms of a neurological disease/disorder or a neurodegenerative disorder, a muscular disorder or neuromuscular disorder, and/or a neuro-oncological disorder in a subject.

Definitions

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the invention pertains.

Articles such as “a,” “an,” and “the” may mean one or more than one unless indicated to the contrary or otherwise evident from the context. Claims or descriptions that include “or” between one or more members of a group are considered satisfied if one, more than one, or all of the group members are present in, employed in, or otherwise relevant to a given product or process unless indicated to the contrary or otherwise evident from the context. The disclosure includes embodiments in which exactly one member of the group is present in, employed in, or otherwise relevant to a given product or process. The disclosure includes embodiments in which more than one, or the entire group members are present in, employed in, or otherwise relevant to a given product or process.

It is also noted that the term “comprising” is intended to be open and permits but does not require the inclusion of additional elements or steps. When the term “comprising” is used herein, the term “consisting of” and “consisting essentially thereof” is thus also encompassed and disclosed.

Where ranges are given, endpoints are included. Furthermore, it is to be understood that unless otherwise indicated or otherwise evident from the context and understanding of one of ordinary skill in the art, values that are expressed as ranges can assume any specific value or subrange within the stated ranges in different embodiments of the disclosure, to the tenth of the unit of the lower limit of the range, unless the context clearly dictates otherwise.

Adeno-associated virus: As used herein, the term “adeno-associated virus” or “AAV” refers to members of the dependovirus genus or a variant, e.g., a functional variant, thereof. In some embodiments, the AAV is wildtype, or naturally occurring. In some embodiments, the AAV is recombinant.

AAV Particle: As used herein, an “AAV particle” refers to a particle or a virion comprising an AAV capsid, e.g., an AAV capsid variant, and a polynucleotide, e.g., a viral genome or a vector genome. In some embodiments, the viral genome of the AAV particle comprises at least one payload region and at least one ITR. In some embodiments, an AAV particle of the disclosure is an AAV particle comprising an AAV capsid polypeptide, e.g., a parent capsid sequence with at least one peptide, e.g., targeting peptide, insert. In some embodiments, the AAV particle is capable of delivering a nucleic acid, e.g., a payload region, encoding a payload to cells, typically, mammalian, e.g., human, cells. In some embodiments, an AAV particle of the present disclosure may be produced recombinantly. In some embodiments, an AAV particle may be derived from any serotype, described herein or known in the art, including combinations of serotypes (e.g., “pseudotyped” AAV) or from various genomes (e.g., single stranded or self-complementary). In some embodiments, the AAV particle may be replication defective and/or targeted. In some embodiments, the AAV particle may comprises a peptide, e.g., targeting peptide, present, e.g., inserted into, the capsid to enhance tropism for a desired target tissue. It is to be understood that reference to the AAV particle of the disclosure also includes pharmaceutical compositions thereof, even if not explicitly recited.

Administering: As used herein, the term “administering” refers to providing a pharmaceutical agent or composition to a subject.

Amelioration: As used herein, the term “amelioration” or “ameliorating” refers to a lessening of severity of at least one indicator of a condition or disease. For example, in the context of neurodegeneration disorder, amelioration includes the reduction of neuron loss.

Amplicon: As used herein, “amplicon” may refer to any piece of RNA or DNA formed as the product of amplification events, e.g. PCR. In some embodiments, full-length capsid amplicons may be used as templates for next generation sequencing (NGS) library generation. Full-length capsid amplicons may be used for cloning into a DNA library for any number of additional rounds of AAV selection as described herein.

Animal: As used herein, the term “animal” refers to any member of the animal kingdom. In some embodiments, “animal” refers to humans at any stage of development. In some embodiments, “animal” refers to non-human animals at any stage of development. In certain embodiments, the non-human animal is a mammal (e.g., a rodent, a mouse, a rat, a rabbit, a monkey, a dog, a cat, a sheep, cattle, a primate, or a pig). In some embodiments, animals include, but are not limited to, mammals, birds, reptiles, amphibians, fish, and worms. In some embodiments, the animal is a transgenic animal, genetically engineered animal, or a clone.

Antisense strand: As used herein, the term “the antisense strand” or “the first strand” or “the guide strand” of a siRNA molecule refers to a strand that is substantially complementary to a section of about 10-50 nucleotides, e.g., about 15-30, 16-25, 18-23 or 19-22 nucleotides of the mRNA of a gene targeted for silencing. The antisense strand or first strand has sequence sufficiently complementary to the desired target mRNA sequence to direct target-specific silencing, e.g., complementarity sufficient to trigger the destruction of the desired target mRNA by the RNAi machinery or process.

Approximately: As used herein, the term “approximately” or “about,” as applied to one or more values of interest, refers to a value that is similar to a stated reference value. In certain embodiments, the term “approximately” or “about” refers to a range of values that fall within 25%, 20%, 19%, 18%, 17%, 16%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, or less in either direction (greater than or less than) of the stated reference value unless otherwise stated or otherwise evident from the context (except where such number would exceed 100% of a possible value).

Biopanning: As used herein, the term “biopanning” refers to an AAV capsid library selection process comprising administration of an AAV particle with enhanced tissue- and/or cell type-specific transduction to a cell and/or subject; extraction of nucleotides encoded by said AAV particle from said transduced tissue- and/or cell type-specific; and, use of the extracted nucleotides for cloning into a nucleotide library for the generation of AAV particles for subsequent rounds of the same.

Capsid: As used herein, the term “capsid” refers to the exterior, e.g., a protein shell, of a virus particle, e.g., an AAV particle, that is substantially (e.g., >50%, >60%, >70%, >80%, >90%, >95%, >99%, or 100%) protein. In some embodiments, the capsid is an AAV capsid comprising an AAV capsid protein described herein, e.g., a VP1, VP2, and/or VP3 polypeptide. The AAV capsid protein can be a wild-type AAV capsid protein or a variant, e.g., a structural and/or functional variant from a wild-type or a reference capsid protein, referred to herein as an “AAV capsid variant.” In some embodiments, the AAV capsid variant described herein has the ability to enclose, e.g., encapsulate, a viral genome and/or is capable of entry into a cell, e.g., a mammalian cell. In some embodiments, the AAV capsid variant described herein may have modified tropism compared to that of a wild-type AAV capsid, e.g., the corresponding wild-type capsid.

Complementary and substantially complementary: As used herein, the term “complementary” refers to the ability of polynucleotides to form base pairs with one another. Base pairs are typically formed by hydrogen bonds between nucleotide units in antiparallel polynucleotide strands. Complementary polynucleotide strands can form base pairs in the Watson-Crick manner (e.g., A to T, A to U, C to G), or in any other manner that allows for the formation of duplexes. As persons skilled in the art are aware, when using RNA as opposed to DNA, uracil rather than thymine is the base that is considered to be complementary to adenine. However, when a U is denoted in the context of the present disclosure, the ability to substitute a T is implied, unless otherwise stated. Perfect complementarity or 100% complementarity refers to the situation in which each nucleotide unit of one polynucleotide strand can form a hydrogen bond with a nucleotide unit of a second polynucleotide strand. Less than perfect complementarity refers to the situation in which some, but not all, nucleotide units of two strands can form hydrogen bond with each other. For example, for two 20-mers, if only two base pairs on each strand can form a hydrogen bond with each other, the polynucleotide strands exhibit 10% complementarity. In the same example, if 18 base pairs on each strand can form hydrogen bonds with each other, the polynucleotide strands exhibit 90% complementarity. The term “complementary” as used herein can encompass fully complementary, partially complementary, or substantially complementary. As used herein, the term “substantially complementary” means that the siRNA has a sequence (e.g., in the antisense strand) which is sufficient to bind the desired target mRNA, and to trigger the RNA silencing of the target mRNA. “Fully complementary”, “perfect complementarity”, or “100% complementarity” refers to the situation in which each nucleotide unit of one polynucleotide or oligonucleotide strand can base-pair with a nucleotide unit of a second polynucleotide or oligonucleotide strand.

Control Elements: As used herein, “control elements”, “regulatory control elements” or “regulatory sequences” refers to promoter regions, polyadenylation signals, transcription termination sequences, upstream regulatory domains, origins of replication, internal ribosome entry sites (“IRES”), enhancers, and the like, which provide for the replication, transcription and translation of a coding sequence in a recipient cell. Not all of these control elements need always be present as long as the selected coding sequence is capable of being replicated, transcribed and/or translated in an appropriate host cell.

Delivery: As used herein, “delivery” refers to the act or manner of delivering an AAV particle, a compound, substance, entity, moiety, cargo or payload.

Element: As used herein, the term “element” refers to a distinct portion of an entity. In some embodiments, an element may be a polynucleotide sequence with a specific purpose, incorporated into a longer polynucleotide sequence.

Encapsulate: As used herein, the term “encapsulate” means to enclose, surround or encase. As an example, a capsid protein, e.g., an AAV capsid variant, often encapsulates a viral genome. In some embodiments, encapsulate within a capsid, e.g., an AAV capsid variant, encompasses 100% coverage by a capsid, as well as less than 100% coverage, e.g., 95%, 90%, 85%, 80%, 70%, 60% or less. For example, gaps or discontinuities may be present in the capsid so long as the viral genome is retained in the capsid, e.g., prior to entry into a cell.

Engineered: As used herein, embodiments of the disclosure are “engineered” when they are designed to have a feature or property, whether structural or chemical, that varies from a starting point, wild type or native molecule.

Effective Amount: As used herein, the term “effective amount” of an agent is that amount sufficient to effect beneficial or desired results, for example, clinical results, and, as such, an “effective amount” depends upon the context in which it is being applied. For example, in the context of administering an agent that treats cancer, an effective amount of an agent is, for example, an amount sufficient to achieve treatment, as defined herein, of cancer, as compared to the response obtained without administration of the agent.

Expression: As used herein, “expression” of a nucleic acid sequence refers to one or more of the following events: (1) production of an RNA template from a DNA sequence (e.g., by transcription); (2) processing of an RNA transcript (e.g., by splicing, editing, 5′ cap formation, and/or 3′ end processing); (3) translation of an RNA into a polypeptide or protein; and (4) post-translational modification of a polypeptide or protein.

Formulation: As used herein, a “formulation” includes at least one AAV particle (active ingredient) and an excipient, and/or an inactive ingredient.

Fragment: A “fragment,” as used herein, refers to a portion. For example, an antibody fragment may comprise a CDR, or a heavy chain variable region, or a scFv, etc.

Functional: As used herein, a “functional” biological molecule is a biological molecule in a form in which it exhibits a property and/or activity.

Dual-function targeting: As used herein, a “dual-function targeting” modulatory polynucleotide is a polynucleotide where both the guide and passenger strands knock down the same target or the guide and passenger strands knock down different targets.

Gene expression: The term “gene expression” refers to the process by which a nucleic acid sequence undergoes transcription and/or translation to produce a protein or peptide. For clarity, when reference is made to measurement of “gene expression”, this should be understood to mean that measurements may be of the nucleic acid product of transcription, e.g., RNA or mRNA or of the amino acid product of translation, e.g., polypeptides or peptides. Methods of measuring the amount or levels of RNA, mRNA, polypeptides and peptides are known in the art.

Homology: As used herein, the term “homology” refers to the overall relatedness between polymeric molecules, e.g. between polynucleotide molecules (e.g. DNA molecules and/or RNA molecules) and/or between polypeptide molecules. In some embodiments, polymeric molecules are considered to be “homologous” to one another if their sequences are at least 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 99% identical or similar. The term “homologous” necessarily refers to a comparison between at least two sequences (polynucleotide or polypeptide sequences). In accordance with the disclosure, two polynucleotide sequences are considered to be homologous if the polypeptides they encode are at least about 50%, 60%, 70%, 80%, 90%, 95%, or even 99% for at least one stretch of at least about 20 amino acids. In some embodiments, homologous polynucleotide sequences are characterized by the ability to encode a stretch of at least 4-5 uniquely specified amino acids. For polynucleotide sequences less than 60 nucleotides in length, homology is determined by the ability to encode a stretch of at least 4-5 uniquely specified amino acids. In accordance with the disclosure, two protein sequences are considered to be homologous if the proteins are at least about 50%, 60%, 70%, 80%, or 90% identical for at least one stretch of at least about 20 amino acids.

Identity: As used herein, the term “identity” refers to the overall relatedness between polymeric molecules, e.g., between polynucleotide molecules (e.g. DNA molecules and/or RNA molecules) and/or between polypeptide molecules. Calculation of the percent identity of two polynucleotide sequences, for example, can be performed by aligning the two sequences for optimal comparison purposes (e.g., gaps can be introduced in one or both of a first and a second nucleic acid sequences for optimal alignment and non-identical sequences can be disregarded for comparison purposes). In certain embodiments, the length of a sequence aligned for comparison purposes is at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, or 100% of the length of the reference sequence. The nucleotides at corresponding nucleotide positions are then compared. When a position in the first sequence is occupied by the same nucleotide as the corresponding position in the second sequence, then the molecules are identical at that position. The percent identity between the two sequences is a function of the number of identical positions shared by the sequences, taking into account the number of gaps, and the length of each gap, which needs to be introduced for optimal alignment of the two sequences. The comparison of sequences and determination of percent identity between two sequences can be accomplished using a mathematical algorithm. For example, the percent identity between two nucleotide sequences can be determined using methods such as those described in Computational Molecular Biology, Lesk, A. M., ed., Oxford University Press, New York, 1988; Biocomputing: Informatics and Genome Projects, Smith, D. W., ed., Academic Press, New York, 1993; Sequence Analysis in Molecular Biology, von Heinje, G., Academic Press, 1987; Computer Analysis of Sequence Data, Part I, Griffin, A. M., and Griffin, H. G., eds., Humana Press, New Jersey, 1994; and Sequence Analysis Primer, Gribskov, M. and Devereux, J., eds., M Stockton Press, New York, 1991; the contents of each of which are incorporated herein by reference in their entirety. For example, the percent identity between two nucleotide sequences can be determined using the algorithm of Meyers and Miller (CABIOS, 1989, 4:11-17), which has been incorporated into the ALIGN program (version 2.0) using a PAM120 weight residue table, a gap length penalty of 12 and a gap penalty of 4. The percent identity between two nucleotide sequences can, alternatively, be determined using the GAP program in the GCG software package using an NWSgapdna.CMP matrix. Methods commonly employed to determine percent identity between sequences include, but are not limited to those disclosed in Carillo, H., and Lipman, D., SIAM J Applied Math., 48:1073 (1988); incorporated herein by reference. Techniques for determining identity are codified in publicly available computer programs. Exemplary computer software to determine homology between two sequences include, but are not limited to, GCG program package, Devereux, J., et al., Nucleic Acids Research, 12(1), 387 (1984)), BLASTP, BLASTN, and FASTA Altschul, S. F. et al., J. Molec. Biol., 215, 403 (1990)).

Inhibit expression of a gene: As used herein, the phrase “inhibit expression of a gene” means to cause a reduction in the amount of an expression product of the gene. The expression product can be an RNA transcribed from the gene (e.g., an mRNA) or a polypeptide translated from an mRNA transcribed from the gene. Typically, a reduction in the level of an mRNA results in a reduction in the level of a polypeptide translated therefrom. The level of expression may be determined using standard techniques for measuring mRNA or protein.

Insert: As used herein the term “insert,” when referring to a polypeptide, refers to the addition of one or more amino acids, e.g., a peptide, e.g., targeting peptide, sequence to an amino acid sequence, e.g., a parent AAV capsid sequence. In some embodiments, an insertion may result in the replacement of one or more amino acids of the amino acid sequence, e.g., the parent AAV capsid sequence. In some embodiments, an insertion may result in no changes to the amino acid sequence, e.g., parent AAV capsid sequence, beyond the addition of the one or more amino acids, e.g., a peptide, e.g., targeting peptide sequence.

Inverted terminal repeat: As used herein, the term “inverted terminal repeat” or “ITR” refers to a cis-regulatory element for the packaging of polynucleotide sequences into viral capsids.

Isolated: As used herein, the term “isolated” refers to a substance or entity that is altered or removed from the natural state, e.g., altered or removed from at least some of component with which it is associated in the natural state. For example, a nucleic acid or a peptide naturally present in a living animal is not “isolated,” but the same nucleic acid or peptide partially or completely separated from the coexisting materials of its natural state is “isolated.” An isolated nucleic acid or protein can exist in substantially purified form, or can exist in a non-native environment such as, for example, a host cell. Such polynucleotides could be part of a vector and/or such polynucleotides or polypeptides could be part of a composition, and still be isolated in that such vector or composition is not part of the environment in which it is found in nature. In some embodiments, an isolated nucleic acid is recombinant, e.g., incorporated into a vector.

Library: As used herein, the term “library” refers to a diverse collection of linear polypeptides, polynucleotides, viral particles, or viral vectors. As examples, a library may be a DNA library or an AAV capsid library.

Molecular scaffold: As used herein a “molecular scaffold” is a framework or starting molecule that forms the sequence or structural basis against which to design or make a subsequent molecule.

Neurological disease: As used herein, a “neurological disease” is any disease associated with the central or peripheral nervous system and components thereof (e.g., neurons).

Naturally Occurring: As used herein, “naturally occurring” or “wild-type” refers to a substance or entity that has not been altered, e.g., structurally altered, or removed from the natural state, e.g., removed from at least some of component with which it is associated in the natural state. In some embodiments, a naturally occurring when referring to sequence refers to a sequence identical to a wild-type sequence or a naturally occurring variant thereof.

Orthogonal evolution: As used herein, the term “orthogonal evolution” refers to a method wherein AAV particles are administered for a first round of AAV selection as described herein across a set of any number of cell- and/or subject-types that may be from different species and/or strains, and wherein any number of additional, i.e., subsequent, AAV selection rounds are performed either across a set of any number of cell- and/or subject-types that may be from different species and/or strains, or across a set of any number of cell- and/or subject-types that may be from the same species and/or strain.

Open reading frame: As used herein, “open reading frame” or “ORF” refers to a sequence which does not contain a stop codon in a given reading frame.

Parent sequence: As used herein, a “parent sequence” is a nucleic acid or amino acid sequence from which a variant is derived. In some embodiments, a parent sequence is a sequence into which a heterologous sequence is inserted. In other words, a parent sequence may be considered an acceptor or recipient sequence. In some embodiments, a parent sequence is an AAV capsid sequence into which a targeting sequence is inserted.

Particle: As used herein, a “particle” is a virus comprised of at least two components, a protein capsid and a polynucleotide sequence enclosed within the capsid.

Patient: As used herein, “patient” refers to a subject who may seek or be in need of treatment, requires treatment, is receiving treatment, will receive treatment, or a subject who is under care by a trained professional for a particular disease or condition.

Payload region: As used herein, a “payload region” is any nucleic acid sequence (e.g., within the viral genome) which encodes one or more “payloads” of the disclosure. As non-limiting examples, a payload region may be a nucleic acid sequence within the viral genome of an AAV particle, which encodes a payload, wherein the payload is an RNAi agent or a polypeptide. Payloads of the present disclosure may be, but are not limited to, peptides, polypeptides, proteins, antibodies, RNAi agents, etc.

Polypeptide: As used herein, “polypeptide” means a polymer of amino acid residues (natural or unnatural) linked together most often by peptide bonds. The term, as used herein, refers to proteins, polypeptides, and peptides of any size, structure, or function. In some instances, the polypeptide encoded is smaller than about 50 amino acids and the polypeptide is then termed a peptide. If the polypeptide is a peptide, it will be at least about 2, 3, 4, or at least 5 amino acid residues long. Thus, polypeptides include gene products, naturally occurring polypeptides, synthetic polypeptides, homologs, orthologs, paralogs, fragments and other equivalents, variants, and analogs of the foregoing. A polypeptide may be a single molecule or may be a multi-molecular complex such as a dimer, trimer or tetramer. They may also comprise single chain or multichain polypeptides and may be associated or linked. The term polypeptide may also apply to amino acid polymers in which one or more amino acid residues are an artificial chemical analogue of a corresponding naturally occurring amino acid.

Polypeptide variant: The term “polypeptide variant” refers to molecules which differ in their amino acid sequence from a native or reference sequence. The amino acid sequence variants may possess substitutions, deletions, and/or insertions at certain positions within the amino acid sequence, as compared to a native or reference sequence. In some embodiments, a variant comprises a sequence having at least about 50%, at least about 80%, or at least about 90%, identical (homologous) to a native or a reference sequence.

Peptide: As used herein, “peptide” is less than or equal to 50 amino acids long, e.g., about 5, 10, 15, 20, 25, 30, 35, 40, 45, or 50 amino acids long.

Pharmaceutically acceptable: The phrase “pharmaceutically acceptable” is employed herein to refer to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problem or complication, commensurate with a reasonable benefit/risk ratio.

Preventing: As used herein, the term “preventing” or “prevention” refers to partially or completely delaying onset of an infection, disease, disorder and/or condition; partially or completely delaying onset of one or more symptoms, features, or clinical manifestations of a particular infection, disease, disorder, and/or condition; partially or completely delaying onset of one or more symptoms, features, or manifestations of a particular infection, disease, disorder, and/or condition; partially or completely delaying progression from an infection, a particular disease, disorder and/or condition; and/or decreasing the risk of developing pathology associated with the infection, the disease, disorder, and/or condition.

Prophylactic: As used herein, “prophylactic” refers to a therapeutic or course of action used to prevent the spread of disease.

Prophylaxis: As used herein, a “prophylaxis” refers to a measure taken to maintain health and prevent the spread of disease.

Region: As used herein, the term “region” refers to a zone or general area. In some embodiments, when referring to a protein or protein module, a region may comprise a linear sequence of amino acids along the protein or protein module or may comprise a three-dimensional area, an epitope and/or a cluster of epitopes. In some embodiments, regions comprise terminal regions. As used herein, the term “terminal region” refers to regions located at the ends or termini of a given agent. When referring to proteins, terminal regions may comprise N- and/or C-termini.

In some embodiments, when referring to a polynucleotide, a region may comprise a linear sequence of nucleic acids along the polynucleotide or may comprise a three-dimensional area, secondary structure, or tertiary structure. In some embodiments, regions comprise terminal regions. As used herein, the term “terminal region” refers to regions located at the ends or termini of a given agent. When referring to polynucleotides, terminal regions may comprise 5′ and/or 3′ termini.

RNA or RNA molecule: As used herein, the term “RNA” or “RNA molecule” or “ribonucleic acid molecule” refers to a polymer of ribonucleotides; the term “DNA” or “DNA molecule” or “deoxyribonucleic acid molecule” refers to a polymer of deoxyribonucleotides. DNA and RNA can be synthesized naturally, e.g., by DNA replication and transcription of DNA, respectively; or be chemically synthesized. DNA and RNA can be single-stranded (i.e., ssRNA or ssDNA, respectively) or multi-stranded (e.g., double stranded, i.e., dsRNA and dsDNA, respectively). The term “mRNA” or “messenger RNA”, as used herein, refers to a single stranded RNA that encodes the amino acid sequence of one or more polypeptide chains.

RNA interfering or RNAi: As used herein, the term “RNA interfering” or “RNAi” refers to a sequence specific regulatory mechanism mediated by RNA molecules which results in the inhibition or interfering or “silencing” of the expression of a corresponding protein-coding gene. RNAi has been observed in many types of organisms, including plants, animals and fungi. RNAi occurs in cells naturally to remove foreign RNAs (e.g., viral RNAs). Natural RNAi proceeds via fragments cleaved from free dsRNA which direct the degradative mechanism to other similar RNA sequences. RNAi is controlled by the RNA-induced silencing complex (RISC) and is initiated by short/small dsRNA molecules in cell cytoplasm, where they interact with the catalytic RISC component argonaute. The dsRNA molecules can be introduced into cells exogenously. Exogenous dsRNA initiates RNAi by activating the ribonuclease protein Dicer, which binds and cleaves dsRNAs to produce double-stranded fragments of 21-25 base pairs with a few unpaired overhang bases on each end. These short double stranded fragments are called small interfering RNAs (siRNAs).

RNAi agent: As used herein, the term “RNAi agent” refers to an RNA molecule, or its derivative, that can induce inhibition, interfering, or “silencing” of the expression of a target gene and/or its protein product. An RNAi agent may knock-out (virtually eliminate or eliminate) expression, or knock-down (lessen or decrease) expression. The RNAi agent may be, but is not limited to, dsRNA, siRNA, shRNA, pre-miRNA, pri-miRNA, miRNA, stRNA, lncRNA, piRNA, or snoRNA.

miR binding site: As used herein, a “miR binding site” comprises a nucleic acid sequence (whether RNA or DNA, e.g., differ by “U” of RNA or “T” in DNA) that is capable of binding, or binds, in whole or in part to a microRNA (miR) through complete or partial hybridization. Typically, such binding occurs between the miR and the miR binding site in the reverse complement orientation. In some embodiments, the miR binding site is transcribed from the AAV vector genome encoding the miR binding site.

In some embodiments, a miR binding site may be encoded or transcribed in series. Such a “miR binding site series” or “miR BSs” may include two or more miR binding sites having the same or different nucleic acid sequence.

Spacer: As used here, a “spacer” is generally any selected nucleic acid sequence of, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides in length, which is located between two or more consecutive miR binding site sequences. Spacers may also be more than 10 nucleotides in length, e.g., 20, 30, 40, or 50 or more than 50 nucleotides.

Sample: As used herein, the term “sample” or “biological sample” refers to a subset of its tissues, cells, nucleic acids, or component parts (e.g. body fluids, including but not limited to blood, serum, mucus, lymphatic fluid, synovial fluid, cerebrospinal fluid, saliva, amniotic fluid, amniotic cord blood, urine, vaginal fluid and semen). A sample further may include a homogenate, lysate or extract prepared from a whole organism or a subset of its tissues, cells or component parts, or a fraction or portion thereof, including but not limited to, for example, plasma, serum, spinal fluid, lymph fluid, the external sections of the skin, respiratory, intestinal, and genitourinary tracts, tears, saliva, milk, blood cells, tumors, organs. A sample further refers to a medium, such as a nutrient broth or gel, which may contain cellular components, such as proteins or nucleic acid molecule.

Self-complementary viral particle: As used herein, a “self-complementary viral particle” is a particle comprised of at least two components, a protein capsid and a self-complementary viral genome enclosed within the capsid.

Sense Strand: As used herein, the term “the sense strand” or “the second strand” or “the passenger strand” of a siRNA molecule refers to a strand that is complementary to the antisense strand or first strand. The antisense and sense strands of a siRNA molecule are hybridized to form a duplex structure. As used herein, a “siRNA duplex” includes a siRNA strand having sufficient complementarity to a section of about 10-50 nucleotides of the mRNA of the gene targeted for silencing and a siRNA strand having sufficient complementarity to form a duplex with the other siRNA strand.

Similarity: As used herein, the term “similarity” refers to the overall relatedness between polymeric molecules, e.g. between polynucleotide molecules (e.g. DNA molecules and/or RNA molecules) and/or between polypeptide molecules. Calculation of percent similarity of polymeric molecules to one another can be performed in the same manner as a calculation of percent identity, except that calculation of percent similarity takes into account conservative substitutions as is understood in the art.

Short interfering RNA or siRNA: As used herein, the terms “short interfering RNA,” “small interfering RNA” or “siRNA” refer to an RNA molecule (or RNA analog) comprising between about 5-60 nucleotides (or nucleotide analogs) which is capable of directing or mediating RNAi. Preferably, a siRNA molecule comprises between about 15-30 nucleotides or nucleotide analogs, such as between about 16-25 nucleotides (or nucleotide analogs), between about 18-23 nucleotides (or nucleotide analogs), between about 19-22 nucleotides (or nucleotide analogs) (e.g., 19, 20, 21 or 22 nucleotides or nucleotide analogs), between about 19-25 nucleotides (or nucleotide analogs), and between about 19-24 nucleotides (or nucleotide analogs). The term “short” siRNA refers to a siRNA comprising 5-23 nucleotides, preferably 21 nucleotides (or nucleotide analogs), for example, 19, 20, 21 or 22 nucleotides. The term “long” siRNA refers to a siRNA comprising 24-60 nucleotides, preferably about 24-25 nucleotides, for example, 23, 24, 25 or 26 nucleotides. Short siRNAs may, in some instances, include fewer than 19 nucleotides, e.g., 16, 17 or 18 nucleotides, or as few as 5 nucleotides, provided that the shorter siRNA retains the ability to mediate RNAi. Likewise, long siRNAs may, in some instances, include more than 26 nucleotides, e.g., 27, 28, 29, 30, 35, 40, 45, 50, 55, or even 60 nucleotides, provided that the longer siRNA retains the ability to mediate RNAi or translational repression absent further processing, e.g., enzymatic processing, to a short siRNA. siRNAs can be single stranded RNA molecules (ss-siRNAs) or double stranded RNA molecules (ds-siRNAs) comprising a sense strand and an antisense strand which hybridized to form a duplex structure called an siRNA duplex.

Subject: As used herein, the term “subject” or “patient” refers to any organism to which a composition in accordance with the disclosure may be administered, e.g., for experimental, diagnostic, prophylactic, and/or therapeutic purposes. Typical subjects include animals (e.g., mammals such as mice, rats, rabbits, non-human primates, and humans) and/or plants.

Substantially: As used herein, the term “substantially” refers to the qualitative condition of exhibiting total or near-total extent or degree of a characteristic or property of interest. One of ordinary skill in the biological arts will understand that biological and chemical phenomena rarely, if ever, go to completion and/or proceed to completeness or achieve or avoid an absolute result. The term “substantially” is therefore used herein to capture the potential lack of completeness inherent in many biological and chemical phenomena.

Targeting peptide: As used herein, a “targeting peptide” refers to a peptide of 3-20 amino acids in length. These targeting peptides may be inserted into, or attached to, a parent amino acid sequence to alter the characteristics (e.g., tropism) of the parent protein. As a non-limiting example, the targeting peptide can be inserted into an AAV capsid sequence for enhanced targeting to a desired cell-type, tissue, organ or organism. It is to be understood that a targeting peptide is encoded by a targeting polynucleotide which may similarly be inserted into a parent polynucleotide sequence. Therefore, a “targeting sequence” refers to a peptide or polynucleotide sequence for insertion into an appropriate parent sequence (amino acid or polynucleotide, respectively).

Target Cells: As used herein, “target cells” or “target tissue” refers to any one or more cells of interest. The cells may be found in vitro, in vivo, in situ or in the tissue or organ of an organism. The organism may be an animal, preferably a mammal, more preferably a human and most preferably a patient.

Therapeutic Agent: The term “therapeutic agent” refers to any agent that, when administered to a subject, has a therapeutic, diagnostic, and/or prophylactic effect and/or elicits a desired biological and/or pharmacological effect.

Therapeutically effective amount: As used herein, the term “therapeutically effective amount” means an amount of an agent to be delivered (e.g., nucleic acid, drug, therapeutic agent, diagnostic agent, prophylactic agent, etc.) that is sufficient, when administered to a subject suffering from or susceptible to an infection, disease, disorder, and/or condition, to treat, improve symptoms of, diagnose, prevent, and/or delay the onset of the infection, disease, disorder, and/or condition. In some embodiments, a therapeutically effective amount is provided in a single dose.

Therapeutically effective outcome: As used herein, the term “therapeutically effective outcome” means an outcome that is sufficient in a subject suffering from or susceptible to an infection, disease, disorder, and/or condition, to treat, improve symptoms of, diagnose, prevent, and/or delay the onset of the infection, disease, disorder, and/or condition.

Treating: As used herein, the term “treating” refers to partially or completely alleviating, ameliorating, improving, relieving, delaying onset of, inhibiting progression of, reducing severity of, and/or reducing incidence of one or more symptoms or features of a particular infection, disease, disorder, and/or condition. For example, “treating” cancer may refer to inhibiting survival, growth, and/or spread of a tumor. Treatment may be administered to a subject who does not exhibit signs of a disease, disorder, and/or condition and/or to a subject who exhibits only early signs of a disease, disorder, and/or condition for the purpose of decreasing the risk of developing pathology associated with the disease, disorder, and/or condition.

Variant: As used herein, the term “variant” refers to a polypeptide or polynucleotide that has an amino acid or a nucleotide sequence that is substantially identical, e.g., having at least 70%, 75%, 80%, 85%, 90%, 95% or 99% sequence identity to a reference sequence. In some embodiments, the variant is a functional variant.

Functional Variant: As used herein, the term “functional variant” refers to a polypeptide variant or a polynucleotide variant that has at least one activity of the reference sequence.

Insertional Variant: “Insertional variants” when referring to polypeptides are those with one or more amino acids inserted, e.g., immediately adjacent or subsequent, to a position in an amino acid sequence “Immediately adjacent” or “immediately subsequent” to an amino acid means connected to either the alpha-carboxy or alpha-amino functional group of the amino acid.

Deletional Variant: “Deletional variants” when referring to polypeptides, are those with one or more amino acids in deleted from a reference protein.

Vector: As used herein, the term “vector” refers to any molecule or moiety which transports, transduces or otherwise acts as a carrier of a heterologous molecule. In some embodiments, vectors may be plasmids. In some embodiments, vectors may be viruses. An AAV particle is an example of a vector. Vectors of the present disclosure may be produced recombinantly and may be based on and/or may comprise adeno-associated virus (AAV) parent or reference sequences. The heterologous molecule may be a polynucleotide and/or a polypeptide.

Viral Genome: As used herein, the terms “viral genome” or “vector genome” refer to the nucleic acid sequence(s) encapsulated in an AAV particle. A viral genome comprises a nucleic acid sequence with at least one payload region encoding a payload and at least one ITR.

Equivalents and Scope

The disclosures of each and every patent, patent application, and publication cited herein are hereby incorporated herein by reference in their entirety. Those skilled in the art will recognize or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments in accordance with the disclosure described herein. The scope of the present disclosure is not intended to be limited to the above Description, but rather is as set forth in the appended claims.

In addition, it is to be understood that any particular embodiment of the present disclosure that falls within the prior art may be explicitly excluded from any one or more of the claims. Since such embodiments are deemed to be known to one of ordinary skill in the art, they may be excluded even if the exclusion is not set forth explicitly herein. Any particular embodiment of the compositions of the disclosure (e.g., any antibiotic, therapeutic or active ingredient; any method of production; any method of use; etc.) can be excluded from any one or more claims, for any reason, whether or not related to the existence of prior art.

It is to be understood that the words which have been used are words of description rather than limitation, and that changes may be made within the purview of the appended claims without departing from the true scope and spirit of the disclosure in its broader aspects.

While the present disclosure has been described at some length and with some particularity with respect to the several described embodiments, it is not intended that it should be limited to any such particulars or embodiments or any particular embodiment, but it is to be construed with references to the appended claims so as to provide the broadest possible interpretation of such claims in view of the prior art and, therefore, to effectively encompass the intended scope of the disclosure.

The present disclosure is further illustrated by the following non-limiting examples.

EXAMPLES Example 1. Peptide Display Capsid Library Configuration

Peptide display capsid libraries are configured by insertion of randomized n-mer amino acids such as, but not limited to, 5-mer, 6-mer, 7-mer and/or 9-mer amino acids, into the surface-exposed hypervariable loop I, loop IV, loop VI, and/or loop VIII region of any AAV capsid serotype, including AAV5, AAV6, or AAV-DJ8, as well as AAV9 capsids, and/or variants thereof. The genes encoding the peptide display capsid library are under the control of any promotor, depending on the desired tropism, e.g., a neuron-specific synapsin promoter (SYN or Syn), or an astrocyte-specific GFAP promoter.

Peptide display capsid libraries are further configured such that the n-mer peptide insertion(s) follows a contiguous (or continuous) design and/or a noncontiguous (or noncontinuous), or split design, or combination thereof, with insertion position(s) mapped using a sliding window algorithm. As a non-limiting example, the peptide insertion may be an AAV9 6-mer contiguous peptide insertion with a sliding window originating at any amino acid position, e.g., amino acids 454-461. As another non-limiting example, the peptide insertion may be an AAV9 3-mer peptide split design or contiguous peptide insertion with a sliding window originating at any amino acid position, e.g., amino acids 586-588. As yet another non-limiting example, the peptide insertion may be an AAV9 6-mer and/or 7-mer peptide contiguous peptide insertion with a sliding window originating at any amino acid position, e.g., amino acids 585-590.

Any number of such configured peptide display capsid libraries may be pooled in a cell and/or subject, including a non-human primate (NHP) cell and/or subject, and administered to any tissue (e.g., central nervous system tissue) via any route, including but not limited to IV and/or ICM injection, at any VG/cell and/or VG/subject dose. As a non-limiting example, six configured peptide display capsid libraries are pooled and administered to the central nervous system of an NHP via intravenous administration of dose 1×10¹⁴ VG/NHP. As another non-limiting example, six libraries are pooled and administered to the central nervous system of NHP via an intraventricular administration, such as, but not limited intraventricular administration that is an intra-cisterna magna injection (ICM) of dose 2×10¹³ a VG/NHP.

Example 2. Identification and Design of Non-Human Primate AAV Capsid Libraries

A TRACER RNA-driven library selection for increased nervous system tissue transduction in a non-human primate (NHP) is developed and carried out in accordance with methods similar, or equivalent, to those described in WO2020072683, the contents of which are herein incorporated by reference in their entirety, particularly as pertains to the TRACER method.

AAV libraries, e.g., AAV9 libraries, generated are administered by any route to NHPs at a given VG dose(s) per animal. A number of groups of NHPs are administered promoter-driven (e.g., SYN-driven or GFAP-driven) libraries derived from wild-type AAV9 flanking sequences, while other groups receive pooled libraries containing wild-type, PHP.eB-derived, or other AAV serotype sequences. After a period, RNA is extracted from a tissue, such as but not limited to spinal cord and brain tissue. The RNA preparation is subjected to mRNA enrichment. The enriched mRNA is reverse transcribed to cDNA. The cDNA is amplified. This method allows recovery of abundant amplicons from tissue samples.

Full-length capsid amplicons are used as templates for NGS library generation, as well as cloning into a DNA library for the next, or subsequent, round(s) of biopanning (FIG. 1A and FIG. 1B). Any number of rounds of AAV selection may be performed. The total number of unique capsid variants may drop by a fold amount across AAV selection rounds. Capsid libraries may be pooled or combined at any step with any other capsid libraries described herein.

Following RNA recovery and PCR amplification, a systematic enrichment analysis by NGS is performed. Capsids enrichment ratio including comparison to a benchmark and sequence convergence is evaluated.

Peptide library candidates are evaluated and optimized using any of the methods described herein and are carried out, e.g., using methods similar, or equivalent, to those described in WO2020072683, the contents of which are herein incorporated by reference in their entirety, particularly the subject matter of Examples, 8, 9, and 10. The top-ranking peptide variants are generated and transduction efficacy evaluated as described in WO2020072683, the contents of which are herein incorporated by reference in their entirety, particularly the subject matter of Examples 10, 12 and 13.

Example 3. Identification and Design of Orthogonal Evolution AAV Capsid Libraries

This study involves the use of orthogonal evolution wherein AAV particles may be administered for a first round of AAV selection across a set of any number of cell- and/or subject-types that may be from different species and/or strains; and, wherein any number of additional, i.e., subsequent, AAV selection rounds are performed either across a set of any number of cell- and/or subject-types that may be from different species and/or strains, or across a set of any number of cell- and/or subject-types that may be from the same species and/or strains, as represented in FIG. 2 .

ATRACER based RNA-driven library selection for increased nervous system tissue transduction a set of any number of cell- and/or subject-types that may be from different species and/or strain is developed and carried out in accordance with methods similar, or equivalent, to those described in WO2020072683, the contents of which are herein incorporated by reference in their entirety, particularly the subject matter of Example 7. AAV libraries, e.g., AAV9 libraries, generated are administered for a first round of AAV selection (biopanning) by any route to a non-human primate (NHP), a rodent (e.g., a rat), and/or a cell (e.g., a human brain microvascular endothelial cell, or hBMVEC) at a given VG dose(s) per subject and/or cell. A number of groups of NHPs, rodents, and/or cells are administered promoter-driven (e.g., SYN-driven or GFAP-driven) libraries derived from wild-type AAV9 sequences, while other groups receive pooled libraries containing wild-type, PHP.eB-derived, or other AAV serotype sequences. After a period, RNA is extracted from a tissue, such as but not limited to spinal cord and brain tissue. The RNA preparation is subjected to mRNA enrichment. The enriched mRNA is reverse transcribed to cDNA. The cDNA is amplified. This method allows recovery of abundant amplicons from tissue samples.

Full-length capsid amplicons are used as templates for NGS library generation, as well as cloning into DNA libraries for the next, or subsequent round(s) of biopanning Subsequent rounds of biopanning are performed either across a set of any number of cell- and/or subject-types that may be from different species and/or strain as used in the above-described first round, or across a set of any number of cell- and/or subject-types that may be from the same species and/or strain as used in the above-described first round. Any number of rounds of selection is performed. The total number of unique capsid variants may drop by a fold amount across AAV selection rounds. Capsid libraries may be pooled or combined at any step with any other capsid libraries described herein (FIG. 2 )

Following RNA recovery and PCR amplification, a systematic enrichment analysis by NGS is performed. Capsids enrichment ratio including comparison to a benchmark and sequence convergence is evaluated.

Peptide library candidates are evaluated and optimized using any of the methods described herein and are carried out, e.g., using methods similar, or equivalent, to those described in WO2020072683, the contents of which are herein incorporated by reference in their entirety. The top-ranking peptide variants are generated and transduction efficacy evaluated as in WO2020072683.

Example 4. NHP High-Throughput Screen of TRACER AAV Libraries

A TRACER based method as described in WO2020072683, the contents of which are herein incorporated by reference in their entirety, was adapted for use in non-human primates (NHP). An orthogonal evolution approach as exemplified in FIG. 2 (e.g., NHP and BMVEC) was combined with a high throughput screening by NGS in NHP as shown in FIG. 3 . Briefly, AAV9/AAV5 starting libraries, driven by synapsin or GFAP promoters as shown in FIG. 4 were administered to non-human primate (NHP) intravenously for in vivo AAV selection (biopanning), performed iteratively. All libraries were injected intravenously at a dose of 1e14VG per animal (approximately 3e13 VG/kg). Orthogonally, biopanning was conducted in hBMVEC cells using the same starting libraries. In the second round of biopanning in NHP, only libraries driven by the synapsin promoter were used. After a period, (e.g., 1 month) RNA was extracted from nervous tissue, e.g., brain and spinal cord. Following RNA recovery and RT-PCR amplification, a systematic NGS enrichment analysis was performed and the targeting peptides shown in Table 1 identified. Capsids enrichment ratio, including calculating the ratio of, e.g., P2/P1 reads and comparison to a benchmark (e.g., AAV9) was evaluated.

Candidate library enrichment data in P3 NHP brain for the targeting peptides of Table 1, over benchmark AAV9, are shown in Table 7. Data are provided as fold enrichment. Fifty-one variants showed greater than 10-fold enrichment over AAV9. Variants with 0.0 enrichment over AAV9 are not included in Table 7.

TABLE 7 NHP NGS AAV9 Enrichment SEQ Fold Peptide ID enrichment Sequence NO: over AAV9 PLNGAVHLYA 1725 473.7 AQARDSPKGW 1726 214 LTNGAVRDRP 1727 134.4 VQAFTHDSRG 1728 88.6 AQAYSTDVRM 1729 84.8 AQAYSTDVRI 1730 83.8 AQAFTAAERM 1731 74.9 AQTHLQIGVA 1732 54.6 AQSNAVLSLA 1733 51.6 AQAYSTDERM 1734 41.4 AQAYSTDVRL 1735 31.7 AQATVSTLRM 1736 31.5 AQAYSTDERK 1737 31.2 AQAYSTDMRM 1738 30.4 VVNGAVLHVA 1739 29.8 AQAYSTDVTM 1740 29.7 AQAHLQIGVA 1741 23 FLDPAVSSKA 1742 22.6 AQAYVSTLRM 1743 21.9 AQAQTGPPLK 1744 20.1 EQASRLPTPG 1745 20 AQASVSTMRM 1746 19.7 TDYSAVRLGA 1747 18 TQAYSTDVRM 1748 17.9 AQALPSNERL 1749 17.4 AQAYSTDVRT 1750 16.4 AQSSLPEMVA 1751 16.2 AQAGEQSTRL 1752 16.1 AQASNDVGRA 1753 15.4 AQATFTASEY 1754 15.3 AKAHAGTIYS 1755 14.9 AQARTIDQCC 1756 14.8 AQEYNSNPKA 1757 14.5 AQVVDNSTHA 1758 14.5 AQATLSVPLK 1759 14.4 AQIVMNSLKA 1760 12.5 AQATMSQTMA 1761 12.5 AQALTQDERW 1762 12 AQAQLSTLRP 1763 11.6 AQVVMGISVA 1764 11.4 AQAYTTDVRM 1765 11.4 AQHIDSMRPP 1766 11.3 AQASTGTLRL 1767 11.1 AQHRALDYYA 1768 11 AQARESPRGL 1769 10.9 AQALLAGTRV 1770 10.7 TKIQAVPWNA 1771 10.7 AQASLSSTRP 1772 10.6 AQAMGSRSDQ 1773 10.4 AQAAQGTYRG 1774 10.3 SQENAVFSKA 1775 10.3 AQALSLSTRP 1776 9.8 AQAAAGTLRD 1777 9.7 AQASRLPTPG 1778 9.5 AQAGSLSERG 1779 9.5 AQSKGDGFTA 1780 9.4 GAGTAVTATA 1781 9.3 AQAQGSSSVG 1782 8.8 AQAYSTDARM 1783 8.8 ERAHAVTGLA 1784 8.5 AQAYGLPKGP 1785 8.4 AQAYSTEVRM 1786 8.4 AQAGVSTALH 1787 8.2 AQSYSTDVRM 1788 8.1 AQPLMSHTDA 1789 7.9 AQAAALASRP 1790 7.9 AQAAITSTIS 1791 7.8 AQPANDGLRA 1792 7.5 AQDYSTDVRM 1793 7.4 AQATLGYSTA 1794 7.4 AQATLGTIRV 1795 7.3 AQAGASDMVH 1796 7 AQAVSGTVRS 1797 6.9 GGTLAVVSLA 1798 6.9 AQAYSADVRM 1799 6.8 AQAFAMPKGL 1800 6.6 AQALVSTSRP 1801 6.6 AQASFQQAST 1802 6.6 AQAMTGNDRS 1803 6.3 AQASTQSPPG 1804 6.1 NARSAVESLA 1805 6.1 AQITVSHTTA 1806 6 AQALAGYDKA 1807 6 AQSTSHDTRA 1808 5.9 AQAIQDRTVV 1809 5.8 AQSKTTLTLA 1810 5.7 AQASMGTVRL 1811 5.7 AQHSDTLTRA 1812 5.5 AQKEMYTSVA 1813 5.5 AQASPSQPLL 1814 5.4 AQAYAGTIYS 1815 5.3 AQARSLEPVI 1816 5.3 TQAGVSTAVH 1817 5.2 AQNTLSLSLA 1818 5.2 AQAYVSSVKM 1819 5.2 AQAATSPRLG 1820 5.1 GYLTAVQPQA 1821 5 LNNLAVGMTA 1822 5 AQTVSVHVRA 1823 5 AQINGLVTTA 1824 4.9 AQAAITTTIS 1825 4.8 AQASTFVTTI 1826 4.7 AQALYDNVPL 1827 4.6 AQAAAGTWKG 1828 4.6 AQATTGTLRS 1829 4.6 GQYAADSSYA 1830 4.5 AQAGIATVRT 1831 4.5 AQALGHELRA 1832 4.5 AQAREAIPQG 1833 4.4 AQAMSGTLRM 1834 4.4 AQAVDRVRPP 1835 4.4 AQAPVNNDRG 1836 4.3 AQAQQVAGTM 1837 4.3 AQAEPRDTRA 1838 4.3 AQRLSEQGVA 1839 4.2 AQASEGIQLS 1840 4.1 AQRQGPDPLA 1841 4.1 AQVTLGSAKA 1842 4.1 AQAGASLGLA 1843 4 AQAFTQDERW 1844 4 AQASQTTVRS 1845 4 AQARVSSNGV 1846 3.9 AQGPLSGLRA 1847 3.9 AQAYGGQSLG 1848 3.9 AQANLGTVRQ 1849 3.9 AQARSDTRGL 1850 3.8 AQAGSDGPRL 1851 3.8 TDGAAVVMRA 1852 3.8 SGITAVPLHA 1853 3.8 AQAAAVGHLP 1854 3.7 AQRVEPKWIA 1855 3.7 AQAVASSPYA 1856 3.7 TQYGAVEGQD 1857 3.7 AQAKSHTLEG 1858 3.6 AQTHLQIVVA 1859 3.6 AQKNEHGMLA 1860 3.6 AQITVSHTRA 1861 3.6 AQARLAPKGL 1862 3.6 KTPGAVSTTA 1863 3.6 AQAFSGTIKS 1864 3.6 PLNGAVNLYA 1865 3.5 AQALTQDERC 1866 3.5 AQATAQVQRS 1867 3.4 AQTPALINLA 1868 3.4 AQASDRSPLL 1869 3.4 AQITVSHTMA 1870 3.3 AQATGTHLMG 1871 3.3 LDGGAVVVTA 1872 3.3 LTNGAVRDRA 1873 3.2 AQARGSDLRD 1874 3.2 AQATFGTQRI 1875 3.2 AQALPQTNRP 1876 3.1 AQARSNDPVL 1877 3.1 AQAYLAVQNG 1878 3.1 AQATQSTLRP 1879 3.1 AQALGGFGPQ 1880 3.1 LVGQAVGSRA 1881 3.1 AQSIANVVVA 1882 3 AQASPSVSRP 1883 3 AQTVVVSTTA 1884 3 VKEQAVSVMA 1885 3 AQQATGTFRA 1886 3 AQAQGSSSGG 1887 3 AQAHAVGPQG 1888 3 AQRLETKETA 1889 3 AQLAQGIGVA 1890 3 AQAVQSSFTI 1891 3 AQATYTASEY 1892 2.9 AQTSSQNLKA 1893 2.9 AQLVPSVAMA 1894 2.9 AQASPSAFAG 1895 2.9 AQALALVSAS 1896 2.9 AQASVGTTYT 1897 2.9 AQARVSSSGT 1898 2.9 NSMGAVLGAA 1899 2.9 AQHTDTLTRA 1900 2.9 AQPNLQPRGA 1901 2.8 AQADRHSSIV 1902 2.8 SPSVAVPSQA 1903 2.8 AQPGIVSTIA 1904 2.8 AQAQHSVGLP 1905 2.7 AQTNSGAILA 1906 2.7 AQDSYDVGRA 1907 2.7 EQAQGSSSVG 1908 2.7 AQAGVSTAVQ 1909 2.7 AQARDMLPLQ 1910 2.7 AQAMVGTLRG 1911 2.7 AQPNVVSTLA 1912 2.7 AQAGHVVTSD 1913 2.7 AQAYTTDERM 1914 2.7 TAVSAVQVMA 1915 2.6 AQAAAGTLRV 1916 2.6 VSNEAVHARA 1917 2.6 AQVLPQSLSA 1918 2.6 AQASVSTLRM 1919 2.6 AQAGLLLSVA 1920 2.5 AQANLVTGPL 1921 2.5 AQASQHSSMA 1922 2.5 GYSSAVSSVA 1923 2.5 AQVGVSPAVA 1924 2.5 DGTLAVPFKA 1925 2.5 AQAPPTSTAM 1926 2.5 AQATPANVRG 1927 2.5 AQAGSSNFLS 1928 2.5 AQLLAQDIRA 1929 2.5 AQPSSDGYRA 1930 2.5 AQALIGTLRT 1931 2.5 SVHGAVGILA 1932 2.5 AQPYVVSGAA 1933 2.4 AQWTHNITAA 1934 2.4 PTNAAVRTNA 1935 2.4 AHAYSTDVRM 1936 2.4 PLAAAVGMKA 1937 2.4 AQARDNSVML 1938 2.4 AQAQFPRNGG 1939 2.4 GALNAVNGVA 1940 2.4 AQASHQQGVP 1941 2.4 SYQSAVVPQA 1942 2.3 AQSIMGTIRA 1943 2.3 AQAYVSQAQG 1944 2.3 AQATGNQAHF 1945 2.3 AQVTVGTPIA 1946 2.3 AQAQTSTFRG 1947 2.3 SVHMAVTVSA 1948 2.2 AQAQSTLNLG 1949 2.2 AQDQTGPPLK 1950 2.2 HLAHAVSTAA 1951 2.2 AQALARDSSF 1952 2.2 AQLLSGTLKA 1953 2.2 AQASLLPTPG 1954 2.2 AQPMAGQSTA 1955 2.2 AQARSLEPDI 1956 2.2 AQFVTGNQDA 1957 2.2 AQATFKTSVP 1958 2.1 LNARAVEGPA 1959 2.1 AQALPNSGRP 1960 2.1 AQALNGSPEA 1961 2.1 AQATSLHPLP 1962 2 AQAVQPPLKN 1963 2 AQAMLSGTRI 1964 2 AQHVDLASKA 1965 2 AQASFATMRP 1966 2 AQAMPLNARS 1967 2 AQALVGQMRG 1968 2 VVNGAVLHLA 1969 2 AQAQTAPPLK 1970 2 AQGHGDLHRA 1971 2 AQAADRSPVH 1972 2 GALNAVTGVA 1973 2 AQAERMASLG 1974 1.9 AQAPPTTTRL 1975 1.9 AQAAVGQTLA 1976 1.9 AQSLGTGMHA 1977 1.9 AQSLGSPALA 1978 1.9 AQASVSVTRP 1979 1.9 AQATMSHTMA 1980 1.9 AQAVQSLTVG 1981 1.9 AQSQTGTYRA 1982 1.9 AQSLASVYAA 1983 1.9 STKLAVHEQA 1984 1.9 AQSHLFPTPA 1985 1.9 AQGTWSSSEA 1986 1.9 AQTPQGLTKA 1987 1.9 AQVSLGTQYA 1988 1.9 AQDSRLPTPG 1989 1.8 ASIQAVGVKA 1990 1.8 AQATMSEQRL 1991 1.8 TAQAAVQGMA 1992 1.8 AQAFNAAERM 1993 1.8 AQINFLSGVA 1994 1.8 PQHLAVSSEA 1995 1.8 AQALGNFPAV 1996 1.8 AQANASTVRV 1997 1.8 AQRIVDLTTA 1998 1.8 VRQVAVEGVA 1999 1.8 AQAPASSQKL 2000 1.8 AQQIDSMRPA 2001 1.7 AQAHGTSSLF 2002 1.7 AQVNSGIALA 2003 1.7 AQLHLAETRA 2004 1.7 AQALTHDERW 2005 1.7 NTVRAVIMEA 2006 1.7 AQAYVAGSRP 2007 1.7 AQDRAFVVSA 2008 1.7 AQAQEKQVFS 2009 1.7 AQACVSTAVH 2010 1.7 AQAFTHDSRG 2011 1.7 AQASHQGTVG 2012 1.7 AQAVLVTEQG 2013 1.7 AQAVVSTAVH 2014 1.7 AQATSRETKG 2015 1.7 YQQPAVSSRA 2016 1.6 AQANMGLSLS 2017 1.6 AQWTSSMSEA 2018 1.6 AQASISIMST 2019 1.6 AQASVAPLTC 2020 1.6 AQLVTVEKQA 2021 1.6 AQAATAGEKL 2022 1.6 AQALSHGPGG 2023 1.6 AQSNAHIEIA 2024 1.6 AQARSSSTGI 2025 1.6 AQAVGGDVTR 2026 1.6 AQAPRTVYQG 2027 1.6 AQALHNLGPA 2028 1.6 VRMGAVSDNA 2029 1.6 AQAFRTSQFT 2030 1.6 AQSSATMQRA 2031 1.5 AQTLAETYRA 2032 1.5 AQANGSIVLN 2033 1.5 AQARVADQLP 2034 1.5 AQAVKQGLYE 2035 1.5 AQAFSDGLKS 2036 1.5 AQVSVTPVKA 2037 1.5 VNGRAVSMMA 2038 1.5 SLVGAVAQMA 2039 1.5 AQARVSPVGL 2040 1.5 AQSNTTLTLA 2041 1.5 AQTSTEHLRA 2042 1.5 AQAGMGINLP 2043 1.5 AQANAHSLTL 2044 1.5 AQARFTTTEM 2045 1.5 AQLGYQEVKA 2046 1.4 AQAGQHASVF 2047 1.4 AQATGSNPRG 2048 1.4 AQAPVSPSIP 2049 1.4 AQTTLGVGTA 2050 1.4 AQASHLVSLA 2051 1.4 AQAPLTGLSV 2052 1.4 AQVSTSTLRA 2053 1.4 AQVQLGTLKA 2054 1.4 AQAHVSVSER 2055 1.4 AQLLLSGQTA 2056 1.4 TTSSAVLTPA 2057 1.4 AQFGADTVNA 2058 1.4 AQTFSSDNLA 2059 1.4 AQIHPANSRA 2060 1.4 AQSIGQFPVA 2061 1.3 AQVISPENLA 2062 1.3 AQALSAISAT 2063 1.3 AQAGVSASQM 2064 1.3 AQASTKTPLP 2065 1.3 AQAPPSTTAM 2066 1.3 AQAVSSDRMH 2067 1.3 AQAGSVTMRL 2068 1.3 AQAVLLGGAV 2069 1.3 AQAQRDMVTT 2070 1.3 AQAHHGSSLG 2071 1.3 VLSSAVGQRA 2072 1.3 AQAAGSVLLG 2073 1.3 AQAYPTDVRM 2074 1.3 AQWSRDAQSA 2075 1.3 AQQGLDMGRA 2076 1.3 AQAAQNHALV 2077 1.3 AQRSQIVEVA 2078 1.3 AQMSDVSGRA 2079 1.3 AKALTQDERW 2080 1.3 AQAVSSSTLT 2081 1.3 AQPSRLPTPG 2082 1.3 RTSTAVLDFA 2083 1.3 AQDLSSSIRA 2084 1.3 AQLLDGLTSA 2085 1.3 AQALIGLSKP 2086 1.3 AQASGTVRPP 2087 1.2 AQLLDTRYKA 2088 1.2 AQAPNTSFTA 2089 1.2 AQTHLQIGVD 2090 1.2 AQRLDTSQVA 2091 1.2 AQRTQDTLSA 2092 1.2 AQADIQSHAL 2093 1.2 PNMNAVGIKA 2094 1.2 AQAGVSTAVH 2095 1.2 AQASGKTFIG 2096 1.2 AQAGVQSTRL 2097 1.2 AQAQGAYPLV 2098 1.2 AQPYSTDVRM 2099 1.2 SSSVAVVTLA 2100 1.2 AQTYNGLNKA 2101 1.2 AQASVSKLRM 2102 1.2 AQRGSENEKA 2103 1.2 PITNAVLKTA 2104 1.2 TNSYAVSSPA 2105 1.2 PYQTAVAGAA 2106 1.1 GPALAVLGRA 2107 1.1 LSISAVPAKA 2108 1.1 AQTLGPLPHA 2109 1.1 AQAQQPLAHV 2110 1.1 AQTDGAWSKA 2111 1.1 AQALSGPPSI 2112 1.1 AQASSPSTRG 2113 1.1 AQASLASNRP 2114 1.1 AQNMALSTVA 2115 1.1 AQHSDTMTRA 2116 1.1 AQAMPRYPPL 2117 1.1 AQHIDSMSPA 2118 1.1 AQALPGTSRV 2119 1.1 AQAKSTQDVQ 2120 1.1 AQPLVSASKA 2121 1.1 AQAMSGTLRK 2122 1.1 AQTLILGAHA 2123 1.1 AQAGQARSQG 2124 1.1 AQRKDLSLVA 2125 1.1 AQALSAPMSL 2126 1.1 TNSLAVGMRA 2127 1.1 AQAPIGTVRP 2128 1.1 FIQAAVSSSA 2129 1.1 AQAEKPTHLL 2130 1.1 AQALSGDTTR 2131 1.1 AQAYIASGGT 2132 1.1 QLNQAVGTLA 2133 1.1 AQASGALDRP 2134 1.1 AQAQDTALRA 2135 1.1 AQAQAGMARG 2136 1.1 AQAQGSSAVG 2137 1.1 AQLLRDIGPA 2138 1.1 VDRGAVTQMA 2139 1.1 AQAVNVSKGS 2140 1.1 SVNTAVESLA 2141 1.1 AQARLPHTSS 2142 1.1 AQRNGSEVVA 2143 1.1 AQATDRVDRP 2144 1.1 AQASLSRERT 2145 1.1 AQAYSTHVRM 2146 1.1 AQHLSAGPTA 2147 1.1 LNGGAVSLRA 2148 1 AQAYGVSSVT 2149 1 AQFGSAVQLA 2150 1 AQAPPTSTRL 2151 1 AQVSTNWPKA 2152 1 AQTSTDLSRA 2153 1 AQAHSTDVRM 2154 1 AQATLTGHVS 2155 1 AQATTQGALT 2156 1 AQAAKASDRT 2157 1 AQGNEHGGRA 2158 1 AQALSTSLLL 2159 1 AQASLGSTYL 2160 1 AQAFSTVGAV 2161 1 AQLNGLVTTA 2162 1 AQASVRTLRM 2163 1 AQATMSRPWQ 2164 1 AQSSLPAMVA 2165 1 RETVAVGQYA 2166 1 AQAFGSEGRS 2167 1 SSGTAVEHRA 2168 1 FGTNAVIPRA 2169 1 AQAGQARSLG 2170 1 AQSFSSDNMA 2171 1 AQMNGLTGKA 2172 1 AQQNGKQHLA 2173 1 AQHIDSIRPA 2174 1 AQAADRLSTL 2175 1 AQFGLKDIRA 2176 1 AQAHQGGATL 2177 1 AQATYNSPKP 2178 1 AQAMSNMLRN 2179 0.9 VPISAVMSTA 2180 0.9 AQHSLGNTVA 2181 0.9 AQATSALSRL 2182 0.9 AQADRQTFPV 2183 0.9 AQAVNSMSIG 2184 0.9 AQALAIVSKN 2185 0.9 AQGQLQERFA 2186 0.9 AQFNGASAHA 2187 0.9 AQLGGQSPVA 2188 0.9 AQANGAYTDN 2189 0.9 AQNLSSSEPA 2190 0.9 AQSAIVLTTA 2191 0.9 ITRSAVPDVA 2192 0.9 GALKAVTGVA 2193 0.9 AQAVGQDYLR 2194 0.9 AQVTLNTPLA 2195 0.9 AQALTQDDRW 2196 0.9 AQAYSTNVRM 2197 0.9 AQAVAAPASL 2198 0.9 AQANPVSIMS 2199 0.9 AQASMQAVKD 2200 0.9 AQAVGGHSVA 2201 0.9 AQAVAASTRL 2202 0.9 GGTHAVSSFA 2203 0.9 AQAADSSGFR 2204 0.9 AQQSHVPQTA 2205 0.9 AQARVGNTNV 2206 0.9 AQTVSYSDLA 2207 0.9 AQAEHGLARS 2208 0.9 AQASNYPVAA 2209 0.9 VLLSAVGMAA 2210 0.9 AQALSGQNRG 2211 0.9 AQAWGQETRQ 2212 0.9 AQGYSTDVRM 2213 0.9 AQAGSVMSRE 2214 0.8 AQAGSLSARG 2215 0.8 AQATALAPKS 2216 0.8 AQAIRQNGSS 2217 0.8 AQLQDNLQLA 2218 0.8 AKAYSTDVRM 2219 0.8 AQSVDRTLLA 2220 0.8 AQAGQNSRLP 2221 0.8 AQTNLQPRGA 2222 0.8 PNTIAVGQRA 2223 0.8 AQAHATLSLS 2224 0.8 NHLRAVGSPA 2225 0.8 AQETDRNLRA 2226 0.8 AQARAETSGS 2227 0.8 AQHRELDSYA 2228 0.8 AQRHTSDVLA 2229 0.8 AQVGQTSSWA 2230 0.8 AQANSAALLM 2231 0.8 AQAIIERTAT 2232 0.8 AQSSRYEEKA 2233 0.8 QATGAVNPRA 2234 0.8 AQASYSVSVG 2235 0.8 AQQGHTVNNA 2236 0.8 AQASLPISTR 2237 0.8 AQHIDSMRPT 2238 0.8 AQAQDTENMR 2239 0.8 RTGAAVTGAA 2240 0.8 AQASSVRGMG 2241 0.8 AQPGIESTIA 2242 0.8 AQHVDLDSKA 2243 0.8 AQATTVPALG 2244 0.8 AQVNPTPQKA 2245 0.8 AQAGYISSAS 2246 0.8 AQTLILGDPA 2247 0.8 AQAASGLTMM 2248 0.8 AQALERPPSG 2249 0.8 TTYDAVHSKA 2250 0.8 AQAMLDSANG 2251 0.7 AQAMHQTDKF 2252 0.7 KSTVAVQSVA 2253 0.7 AQATAGTLIG 2254 0.7 GLKSAVTHVA 2255 0.7 AQAHSAYQGA 2256 0.7 AQSFSSDNLA 2257 0.7 AQLNMAASVA 2258 0.7 AQFSQAYNAA 2259 0.7 AQHLTAGLRA 2260 0.7 AQAHTVSPHL 2261 0.7 GILGAVLPRA 2262 0.7 AQHNSSSLLA 2263 0.7 AQAPQVAGTM 2264 0.7 AQHQDSRPMA 2265 0.7 AQRFQETGLA 2266 0.7 AQLTVSHTRA 2267 0.7 AQANLRTTMG 2268 0.7 AQAGLRDPRM 2269 0.7 AQHLLHGTAA 2270 0.7 RNQGAVASLA 2271 0.7 AQAGSSSVTW 2272 0.7 AQPHLQIGVA 2273 0.7 AQANSGAVLA 2274 0.7 AQLLGDAVKA 2275 0.7 SSGNAVSSLA 2276 0.7 AQVSVTMALA 2277 0.7 AQYHTRGFAA 2278 0.7 AQSTTKGTLA 2279 0.7 AQAQPPSARY 2280 0.7 AQAGLQGTAA 2281 0.7 AQPQGSSTFA 2282 0.7 TQYRAVEGQA 2283 0.7 AQAISTQLAG 2284 0.7 AQLGSNISHA 2285 0.7 AQTGLSGTVA 2286 0.7 AQRVDSSGRA 2287 0.7 AQAGLALNPN 2288 0.7 AQFYSDNSLA 2289 0.7 AQAVGAPQRL 2290 0.7 AQASYDDGRA 2291 0.7 SSFAAVATAA 2292 0.6 AQSTLSMPLA 2293 0.6 AQASLHAPRP 2294 0.6 HAVAAVSYPA 2295 0.6 AQTSPVMVQA 2296 0.6 AQADITSTIS 2297 0.6 AQAAGVAMLY 2298 0.6 AQASVSTLRK 2299 0.6 MDLKAVSSRA 2300 0.6 AQASLSTLRM 2301 0.6 AQPRSPLPMA 2302 0.6 GQYADVSSYA 2303 0.6 LVGGAVVVPA 2304 0.6 AQAQSARPLA 2305 0.6 AQSLHPSTTA 2306 0.6 AQFQTDLSRA 2307 0.6 RTELAVGLSA 2308 0.6 AQVVDNSPLA 2309 0.6 AQAVSSDSMH 2310 0.6 AQASPALHTL 2311 0.6 GQYAAVASYA 2312 0.6 AQLWQSRVDA 2313 0.6 GTFSAVQSTA 2314 0.6 AQAILSTIEV 2315 0.6 AQNVVSTLRA 2316 0.6 AQAMLAVSPG 2317 0.6 AQATDSLVAR 2318 0.6 AQASPQSSHG 2319 0.6 AQATPVHDTL 2320 0.6 LRSSAVGTAA 2321 0.6 MGRGAVLDTA 2322 0.6 AQSHLIPTPA 2323 0.6 AQAVLKAPIN 2324 0.6 AQKIAPAFLA 2325 0.6 AGNVAVLPHA 2326 0.6 AQSLGTGLHD 2327 0.6 AQAHAMSSRP 2328 0.6 AQQGKFDMRA 2329 0.6 AQALSGDGTR 2330 0.6 AQTHLQIAVA 2331 0.6 AQRTQGSSWA 2332 0.6 AIGSAVDLRA 2333 0.6 AQAQLASGTL 2334 0.6 AQALVSAGAL 2335 0.6 AQATESVPLK 2336 0.6 AQVYNSNPKA 2337 0.6 AQRTTYPSSA 2338 0.6 AQAMFQQAST 2339 0.6 AQPDALVIRA 2340 0.6 AQARDISMRG 2341 0.6 AQMSFGSTLA 2342 0.6 RLLSAVDQQA 2343 0.6 AQTTRSIENA 2344 0.6 AQVSDFSSRA 2345 0.5 AQAHSRVNTE 2346 0.5 AQAYSTDLRM 2347 0.5 AQALNGSAYS 2348 0.5 TQYGAVEAQA 2349 0.5 AQAHAGTIYS 2350 0.5 AQDPHSMRPA 2351 0.5 AQASANIHSS 2352 0.5 AQASLQAVSM 2353 0.5 AQSSHPAMVA 2354 0.5 AQANLQPRGA 2355 0.5 AQAVGSSPRG 2356 0.5 AQTSAPSALA 2357 0.5 AQAVQLQNRG 2358 0.5 AQAQGSGMVS 2359 0.5 AQAYPSSKSG 2360 0.5 AQAVSDYGRG 2361 0.5 AQMSLGATRA 2362 0.5 AQAFLNSASA 2363 0.5 AQALPSNARL 2364 0.5 AQANVSVRRE 2365 0.5 AQAGASVMVH 2366 0.5 AQSLAKDQSA 2367 0.5 AQILSALSSA 2368 0.5 AQSVHLSLAA 2369 0.5 AQALSASSFL 2370 0.5 AQTSQLNQTA 2371 0.5 AQSNLFPTPA 2372 0.5 AQAHGRSFDT 2373 0.5 AQLGSNTILA 2374 0.5 AQASMNSAKA 2375 0.5 AQRQAVEQSA 2376 0.5 AQASTGTLRH 2377 0.5 AQGPTYPNVA 2378 0.5 AQAGPTTSKA 2379 0.5 AQATTYRGMA 2380 0.5 AQVTNRGMPA 2381 0.5 AQAISGQAAW 2382 0.5 AQAFRGEDKG 2383 0.5 AQQSMPRFVA 2384 0.5 AQAGVKSTRL 2385 0.5 AQATGSILLA 2386 0.5 AQASGHSSFS 2387 0.5 AQTANDGLRA 2388 0.5 AQASQLALLA 2389 0.5 AQLVDRVPRA 2390 0.5 AQHSNGYVHA 2391 0.5 AQAAPSSSDS 2392 0.5 AQAMQRSSSA 2393 0.5 AQAASGRPTC 2394 0.5 AQPRPGDSRA 2395 0.5 AQRDRANGIA 2396 0.5 AQVLAISLSA 2397 0.5 AQAGMRDPRM 2398 0.5 AQASSNSSRA 2399 0.5 MHRDAVSGVA 2400 0.5 AQAEMKNMPP 2401 0.5 AQSGSLLASA 2402 0.5 AQAFASQSRG 2403 0.5 AQALHLPTLQ 2404 0.5 AQAKTGGMNT 2405 0.5 ISLNAVSGKA 2406 0.5 AQGVHGHYVA 2407 0.5 AQAYSKDVRM 2408 0.5 VPSIAVSSHA 2409 0.5 AQSSRHDDLA 2410 0.5 AQANGSGSRG 2411 0.5 AQVGIADRRA 2412 0.5 AQARGMESML 2413 0.5 AQAGVSTAGH 2414 0.5 AQVSTRNLIA 2415 0.5 AQAVPRLTAG 2416 0.5 AQRHMELQEA 2417 0.5 SQSRAVVWEA 2418 0.5 QSHTAVSSLA 2419 0.5 AKASVSTLRM 2420 0.5 AQASGSSQWA 2421 0.5 AQPNAQYMKA 2422 0.5 AQAMGTGSSL 2423 0.5 AQAFSTSQLT 2424 0.5 AQAKDQSQRL 2425 0.4 AQVGGNGSRA 2426 0.4 AQANGASRAV 2427 0.4 QVNKAVLDFA 2428 0.4 AQETLSSTRA 2429 0.4 GVYGAVHSSA 2430 0.4 AQTITIENVA 2431 0.4 AQALMKIADG 2432 0.4 AQANVSLQAA 2433 0.4 AQSTTSHLRA 2434 0.4 AQLSNLVSVA 2435 0.4 AQANSTPTRQ 2436 0.4 AQQRGDRAAA 2437 0.4 AQARLGQSVG 2438 0.4 AQQLTYGSSA 2439 0.4 AQPAEKQYSA 2440 0.4 AQAMPRSRGD 2441 0.4 AQGLSGRALA 2442 0.4 AQARVTAVDA 2443 0.4 AQVGVSTAVA 2444 0.4 AQTGVTSAQA 2445 0.4 AQALVTSSEK 2446 0.4 AQASPHSSMA 2447 0.4 AQALTQDEMW 2448 0.4 AQAFSTQQRL 2449 0.4 AQAGSQVTQA 2450 0.4 AQQSTLALKA 2451 0.4 AQALNGSHAA 2452 0.4 AQATEGHLRS 2453 0.4 AQPMANMLMA 2454 0.4 PSTSAVSQKA 2455 0.4 AQAPPSSTEM 2456 0.4 AQRERVDLAA 2457 0.4 AQASVTLPRT 2458 0.4 AQAYPSSSKA 2459 0.4 AQAHSGSAIP 2460 0.4 AQSPSQSLKA 2461 0.4 AQATPPATSP 2462 0.4 CLGAAVNQCA 2463 0.4 VLGQAVRDKA 2464 0.4 AQAQKANNVG 2465 0.4 AQTLLPVNGA 2466 0.4 AQAHGTIQRG 2467 0.4 TVYTAVGVSA 2468 0.4 LGRGAVLDMA 2469 0.4 AQANVRSDQM 2470 0.4 AQARDSQKGW 2471 0.4 AQTPGSRSAA 2472 0.4 AQALPSNARQ 2473 0.4 AQASATSVVH 2474 0.4 AQINLGTMRA 2475 0.4 AQVYNNTSAA 2476 0.4 AQASANLTRG 2477 0.4 AQLRTDYTRA 2478 0.4 AQAYSTDVKM 2479 0.4 AQTSQLYQPA 2480 0.4 AQALTQEERW 2481 0.4 LPNGAVRDRA 2482 0.4 VTGSAVAGIA 2483 0.4 AQAFSTDVRM 2484 0.4 AQAHGPTSGV 2485 0.4 AQAGVGLPIA 2486 0.4 AQVNSGQARA 2487 0.4 AQAQTGPPMK 2488 0.4 AQARLAPVAC 2489 0.3 LSIGAVASMA 2490 0.3 AQAQDLGVMR 2491 0.3 PTGLAVTSPA 2492 0.3 AQSASTSWSA 2493 0.3 AQNGSNVRNA 2494 0.3 AQASISSSAT 2495 0.3 AQNSHAHLAA 2496 0.3 AQAVGVKQFF 2497 0.3 AQAHLSPTHA 2498 0.3 AQPAYGSSYA 2499 0.3 AQAHQARSGS 2500 0.3 AQAHTSPTQR 2501 0.3 AQAATPSSSR 2502 0.3 AQAHNSYPKV 2503 0.3 AQSSLGSSLA 2504 0.3 AQALSRSNVG 2505 0.3 AQASLSSLSG 2506 0.3 AQHGSSEFTA 2507 0.3 AQSALVAGVA 2508 0.3 AQASSSSLRP 2509 0.3 AQTARDTGFA 2510 0.3 KSVQAVRDPA 2511 0.3 AQAGSHSSVS 2512 0.3 VRAHAVTGLA 2513 0.3 ASHTAVGEFA 2514 0.3 AQIRSEWRDA 2515 0.3 AQQLARVSGA 2516 0.3 AQAAITSTNS 2517 0.3 AQARDAVQLP 2518 0.3 AQAKELVSTS 2519 0.3 AQGIAETLSA 2520 0.3 AQLGSGFSTA 2521 0.3 AQNAKELERA 2522 0.3 AQTHLQNGVA 2523 0.3 SGNLAVGTPA 2524 0.3 AQPSPGTSVA 2525 0.3 AQSSAAAGRA 2526 0.3 AQAGISAAIM 2527 0.3 AQALGYHQTG 2528 0.3 NAGQAVAARA 2529 0.3 AQPFGGSGYA 2530 0.3 AQAGSPSRLC 2531 0.3 AQARTIGTIA 2532 0.3 AQVVSVSSRA 2533 0.3 AQAGQARSMG 2534 0.3 AQATRGVTAG 2535 0.3 AQQSNGYGRA 2536 0.3 AQASLAPLKS 2537 0.3 AQPGANHNGA 2538 0.3 LGRGAVPDTA 2539 0.3 AQHFQTASLA 2540 0.3 AQAPAGHHTR 2541 0.3 AQPSVQNSMA 2542 0.3 AQAKLSGHVS 2543 0.3 AQFGTSSPSA 2544 0.3 AQASHISSVR 2545 0.3 AQALSRNGIG 2546 0.3 AQASAQVQRS 2547 0.3 AQGGPHLQAA 2548 0.3 AQAQSDSAFR 2549 0.3 AQTYSTDVRM 2550 0.3 LARGAVLDTA 2551 0.3 AQASPHTLRS 2552 0.3 AQHSDTQTRA 2553 0.3 AQATPSPSAS 2554 0.3 AQNQVTYSKA 2555 0.3 AQHTSVVYGA 2556 0.3 AQAQVSQMSH 2557 0.3 SFLRAVKNDA 2558 0.3 AQAYSTDVGM 2559 0.3 AKTPALINLA 2560 0.3 VSTAAVSSAA 2561 0.3 AQAPITSTIS 2562 0.3 AQTNLQTRGA 2563 0.3 AQATRLPTPG 2564 0.3 PQHLAVSSAA 2565 0.2 AQASPHPSRP 2566 0.2 AQAQPAGQRG 2567 0.2 AQPQRQGVQA 2568 0.2 AQHVAGSSNA 2569 0.2 AQVPIQMGVA 2570 0.2 AQATVSVPLK 2571 0.2 AQISVSHTRA 2572 0.2 SLVGPVAQMA 2573 0.2 AQPRLNLTEA 2574 0.2 AQASQEYSRL 2575 0.2 AQKSLAFDSA 2576 0.2 AQALGHSHHC 2577 0.2 AQAAQTGRPI 2578 0.2 AQASGTSVRQ 2579 0.2 AQAMGTASYC 2580 0.2 AQISHNHPQA 2581 0.2 AQAYSTYVRM 2582 0.2 AQVGKLDIRA 2583 0.2 AQLKQGGINA 2584 0.2 AQASAHFREP 2585 0.2 AQALDTVLSA 2586 0.2 GAGTAVGNIA 2587 0.2 AQANGSATYA 2588 0.2 AQTQLAQQKA 2589 0.2 AQYVTTVSPA 2590 0.2 SQFSAVTVTA 2591 0.2 AQAASDSFRY 2592 0.2 AQASPASVTR 2593 0.2 AQARDSGMFL 2594 0.2 AQSKTTLTLS 2595 0.2 AQLVQESLSA 2596 0.2 AQAALKSLAG 2597 0.2 AQAVPNQGQK 2598 0.2 AQALSRSSLG 2599 0.2 AQAGSVMSRV 2600 0.2 AQMATVTPMA 2601 0.2 AQARTASGID 2602 0.2 SHSSAVSHPA 2603 0.2 AQADRMRTTA 2604 0.2 AQNAQNRALA 2605 0.2 AQAASNAYSS 2606 0.2 AQATFQQAST 2607 0.2 AQVYTISTPA 2608 0.2 AQTVIAVGVA 2609 0.2 LARGAVPPTA 2610 0.2 AQMLQTSVLA 2611 0.2 AQARQVSPLL 2612 0.2 AQAGQMSNAR 2613 0.2 AQTPALIKLA 2614 0.2 AQAYTTDVRK 2615 0.2 VVKGAVLHVA 2616 0.2 AQDTVSVPLK 2617 0.2 AQKGAPSLQA 2618 0.2 AQASYDVGRA 2619 0.2 AQGPLSGMRA 2620 0.2 AQALGTSVPA 2621 0.2 AQVNKGASTA 2622 0.2 AQLTRTSPVA 2623 0.2 AQADAALRFS 2624 0.2 AQVQLVVSPA 2625 0.2 AQAYSSDVRM 2626 0.2 AQARSGLSLP 2627 0.2 AQNGHKFTAA 2628 0.2 AQGLSSATKA 2629 0.2 AQGTWSTVKA 2630 0.2 AQASGVGGRI 2631 0.2 AQTSYPAQKA 2632 0.2 AQNAVPTHSA 2633 0.2 AQSYPEITRA 2634 0.2 AQTGLSTSSA 2635 0.2 AQYDTHNFAA 2636 0.2 AQAVLSSVIQ 2637 0.2 AQDSAVALMA 2638 0.2 AQATGKGALP 2639 0.2 AQNSRSGHDA 2640 0.2 AQAFQKEPSV 2641 0.2 AQAGSTSGKM 2642 0.2 AQRDQAHSQA 2643 0.2 AQAASALSGR 2644 0.2 AQARHSSLLP 2645 0.2 AQGPGTSYLA 2646 0.2 FLAPAVSSKA 2647 0.2 AQAGPQCSSC 2648 0.2 AQALTQHERW 2649 0.2 AQAIRSSERV 2650 0.2 AQAVHSSSVY 2651 0.2 AQSSRTALAA 2652 0.2 AQITFSHTRA 2653 0.2 AQALTLSGGL 2654 0.2 AQAGKTLSVL 2655 0.2 AQASRSNLDN 2656 0.2 AQGSLSTHTA 2657 0.2 AQQSVAYNVA 2658 0.2 AQHTLRLSSA 2659 0.2 AQAGGTPNKL 2660 0.2 AQAFQSLTLA 2661 0.2 AQAVALSHQE 2662 0.2 AQMLASGIPA 2663 0.2 AQNRALDSYA 2664 0.2 AQASGSTTRN 2665 0.2 AQARGDGYVA 2666 0.2 DARVAVLDFA 2667 0.2 AQAVASQVSR 2668 0.2 AQARGPSPAT 2669 0.2 AQHRALDSYD 2670 0.2 AQLIDSTSRA 2671 0.2 AQAQTLSRGS 2672 0.2 AQFRSAITSA 2673 0.2 AQANMTKQSL 2674 0.2 AQNAGSTSRA 2675 0.2 VLGSAVTGRA 2676 0.2 AQPMLQSSSA 2677 0.2 AQLGTPSLSA 2678 0.2 AQATAHTGVP 2679 0.2 AQAVGRDNRL 2680 0.2 AQATSASVWA 2681 0.2 AQAGSEASLR 2682 0.2 AQANQNRTAF 2683 0.2 AQASAQVKRS 2684 0.2 AQATSGVHHP 2685 0.2 AQTHMQIGVA 2686 0.2 AQSHIFPTPA 2687 0.2 AQLFHTGSPA 2688 0.2 LASRAVVGTA 2689 0.2 AQALLRVGVG 2690 0.2 AQITLPSGTA 2691 0.2 AQAEKSLGRQ 2692 0.2 AQTSNTTTRA 2693 0.2 AQAHTQASYM 2694 0.2 AQERGASSSA 2695 0.2 AQATPSSTAM 2696 0.2 AQSTVNRTYA 2697 0.2 AQAGHGPSTR 2698 0.2 AQLSLVPLQA 2699 0.2 AQLHSPIPSA 2700 0.2 AQSLARDGLA 2701 0.2 AQAPPSSPAM 2702 0.2 TQYGAVERQA 2703 0.1 AQAGQARSLA 2704 0.1 AQPVGRVPPA 2705 0.1 AQAREQRGPV 2706 0.1 AQKTSLLWEA 2707 0.1 AQGSGKNLIA 2708 0.1 AQASEGHQLS 2709 0.1 AQALHAGHHP 2710 0.1 AQSKRDDPSA 2711 0.1 AQTSRELRMA 2712 0.1 AQALPASGAR 2713 0.1 AQSNALLSLA 2714 0.1 AQASPVVGVS 2715 0.1 AQARGDSYMA 2716 0.1 AQAGASSLTV 2717 0.1 AQALRPVNGT 2718 0.1 AQVRSGPTLA 2719 0.1 AQFPPLSRSA 2720 0.1 AQVARGTVQA 2721 0.1 AQTSTQSPPG 2722 0.1 AQARDGMNVR 2723 0.1 AQAVSRNVVV 2724 0.1 AQHTATRSVA 2725 0.1 AQAVREDGHA 2726 0.1 TNSSAVAASA 2727 0.1 AQATFQLAST 2728 0.1 AQAHHQQTSL 2729 0.1 AQGQHAHMMA 2730 0.1 AQATSSLHVL 2731 0.1 AQAPNSGLAM 2732 0.1 SASRAVLDFA 2733 0.1 AQARGEQRFV 2734 0.1 AQTHLQIRVA 2735 0.1 AQAPPSSKAM 2736 0.1 AQIVSKAMPA 2737 0.1 AQASVRNNPS 2738 0.1 AQAESRVAAL 2739 0.1 LTNGAVRDRT 2740 0.1 AQGRLAGSLA 2741 0.1 AQAGQDSARR 2742 0.1 AQAASRLGAV 2743 0.1 AQALARGMAS 2744 0.1 AQASRGLSMG 2745 0.1 AQAQASSYGS 2746 0.1 AKASRLPTPG 2747 0.1 AQSLSRASTA 2748 0.1 AQASTFVQTI 2749 0.1 AQASSKVVAA 2750 0.1 AQAYRNGEAA 2751 0.1 AQAYSTGVRM 2752 0.1 AQAVSSRSMG 2753 0.1 AQARGGLATP 2754 0.1 AQAGHSGVRA 2755 0.1 AQPSYHGGAA 2756 0.1 AQRVNQVSTA 2757 0.1 AQAAFQQAST 2758 0.1 AQAVPGSPRA 2759 0.1 AQLSLSPLAA 2760 0.1 AQANMTVRVS 2761 0.1 AQATRSSGDP 2762 0.1 AQVASNATRA 2763 0.1 AQTNQQPRGA 2764 0.1 AQRLQNDHLA 2765 0.1 AQAPVQLGRP 2766 0.1 AQRQGPDTLA 2767 0.1 AQHTLSNHMA 2768 0.1 AQLSGMVNRA 2769 0.1 AQDRQVSSRA 2770 0.1 AQRQLSTSLA 2771 0.1 AQQRPTVSFA 2772 0.1 AQAKPHSQLD 2773 0.1 AQAGRVNHPP 2774 0.1 AQAINSQSMR 2775 0.1 AQYSTAVMSA 2776 0.1 SQARAVERSA 2777 0.1 AQAYKSSSVG 2778 0.1 AQASTPGLYP 2779 0.1 AQSRTSMLAA 2780 0.1 AQLFSSNMPA 2781 0.1 AQAYCTDVRM 2782 0.1 AQTMSRGFVA 2783 0.1 AQALNGYPAA 2784 0.1 AQAQTGHPLK 2785 0.1 AQASSNSQYR 2786 0.1 AQAAIKSTIS 2787 0.1 AQSTLNLRPA 2788 0.1 AQATLSPGSG 2789 0.1 AQANGSGTGR 2790 0.1 STSLAVAGRA 2791 0.1 AQASNLSAYR 2792 0.1 AQASRQVLVA 2793 0.1 NEVRAVFFEA 2794 0.1 AKAQGSSSVG 2795 0.1 ARGSAVQSQA 2796 0.1 AVRVAVSSSA 2797 0.1 AQAFSTSQFK 2798 0.1 AQGTSSQRTA 2799 0.1 AQATMSQTIA 2800 0.1 AQSANRSTLA 2801 0.1 AQRDLAHSKA 2802 0.1 AQASKVGLYA 2803 0.1 AQAYYTDVRM 2804 0.1 AQAGLRDPRA 2805 0.1 AQAFSQATGA 2806 0.1 AQVAGMSVRA 2807 0.1 AQAGQSSFTI 2808 0.1 AQKEMRSQGA 2809 0.1 AQNYSSGVRA 2810 0.1 AQITVSYTRA 2811 0.1 AQAQQPRSSI 2812 0.1 WTSGAVPGKA 2813 0.1 AQFKPSQVIA 2814 0.1 RQGQAVGSSA 2815 0.1 AQSISPHYAA 2816 0.1 AQARSLNEYK 2817 0.1 AQAASSRLMA 2818 0.1 AQAYSTDGRM 2819 0.1 AQASVPRVMG 2820 0.1 AQGQMPRYPA 2821 0.1 AQASSGMKPC 2822 0.1 AQPLRSSLSA 2823 0.1 AQNSASQSQA 2824 0.1 AQGHLSGLRA 2825 0.1 AQRAQSGVAA 2826 0.1 AQANPRLQDK 2827 0.1 AQAPRTATLG 2828 0.1 AQRTASLSQA 2829 0.1 AQGNPGLLRA 2830 0.1 MSSHAVGNRA 2831 0.1 AQLAPKASPA 2832 0.1 AQTTQGRERA 2833 0.1 AQASGKSTSS 2834 0.1 AQAPHQHSMK 2835 0.1

A subset of the targeting peptide variants from the NHP biopanning showed a very strong and consistent enrichment over AAV9 and PHP.B controls. Further, the targeting peptide of SEQ ID NO: 1725 not only showed a strong enrichment over AAV9 in the brain, but also in the spinal cord, as it led to a 125.6 fold enrichment over AAV9 in the spinal cord. Following the removal of the least reliable variants, a set of 22 variants with enrichment factors ranging from 7-fold to >400-fold over AAV9 was identified. These were cross-referenced to a non-synthetic PCR-amplified library screened in parallel and 12 candidates showed reliable enrichment and high consistency in both assays. Of these, 5 candidates with the highest enrichment scores in both assays and the highest consistency across animals and tissues were retained for individual evaluation. Candidate capsids were labeled TTD-001, TTD-002, TTD-003, TTD-004 and TTD-005 as shown in Table 3 above.

After 3 rounds of screening of AAV9 peptide insertion library in NHP, many capsids outperformed their parental capsid AAV9 in penetration of the blood brain barrier (BBB). Some of the capsids comprising a targeting peptide showed high enrichment scores and high consistency both across different brain tissue samples from the same animal and across different animals Consistency in both NNK and NNM codons was also observed. 22 capsid variants exhibited enrichment factors ranging from 7-fold to >400-fold over AAV9 in the brain tissues. A majority of these variants also demonstrated high enrichment factors up to 125-fold over AAV9 in the spinal cord. Of these, 5 candidates with diverse inserted sequences were selected for further evaluation as individual capsids.

Example 5. Individual Capsid Characterization

The goal of these experiments was to determine the transduction level and the spatial distribution of each of the 5 capsid candidates selected from the study described in Example 4 relative to AAV9 following intravascular infusion in NHPs (cynomolgus macaque). The 5 selected capsid candidates were TTD-001 (SEQ ID NO: 3623 and 3636, comprising targeting peptide SEQ ID NO: 1725 or 3648), TTD-002 (SEQ ID NO: 3623, 3625, and 3637, comprising targeting peptide SEQ ID NO: 1726 or 3649), TTD-003 (SEQ ID NO: 3626 and 3638, comprising targeting peptide SEQ ID NO: 1729 or 3650), TTD-004 (SEQ ID NO: 3627 and 3639, comprising targeting peptide SEQ ID NO: 1760 or 3651) and TTD-005 (SEQ ID NO: 3629 and 3641, comprising targeting peptide SEQ ID NO: 1769 or 3652) as outlined in Table 3 above.

AAV particles were generated with each of these 5 capsids encapsulating a transgene encoding a payload fused to an HA tag (payload-HA) and driven by a full-length CMV/chicken beta actin promoter by triple transfection in HEK293T cells and formulated in a pharmaceutically acceptable solution.

Each test capsid and AAV9 control were tested by intravenously providing two (2) NHP females the AAV particle formulation at a dose of 2e13 VG/kg. The in-life period was 14 days and then a battery of CNS and peripheral tissues were collected for quantification of transgene mRNA, transgene protein and viral DNA (biodistribution). Samples were also collected, fixed and paraffin embedded for immunohistochemical stainings.

In a first pass screening of RNA quantification by qRT-PCR and RT-ddPCR, total RNA was extracted from 3-mm punches from various areas of the brain (cortex, striatum, hippocampus, cerebellum), spinal cord sections, liver and heart, and analyzed by qRT-PCR using a proprietary Taqman set specific for the synthetic CAG exon-exon junction. Cynomolgus TBP (TATA box-binding protein) was used as a housekeeping gene. Data are shown in FIG. 6A, FIG. 6B, and FIG. 6C.

TRACER capsids showed an increase in RNA expression in all brain regions relative to AAV9 in at least one animal. The highest and most consistent increase in brain transduction was observed with capsids TTD-003 and TTD-004 (8- to 200-fold depending in various anatomical locations). In this initial screening TTD-001 was not assessed due to staggered animal dosing. An approximate 10- to 12-fold increase was consistently observed in whole brain slices (equivalent to an average of multiple regions), which was consistent with the values indicated in a next-generation sequencing (NGS) assay. In order to increase data robustness, droplet digital RT-PCR (ddPCR) was performed in parallel to qRT-PCR and confirmed the trends indicated by the qPCR data as shown in FIG. 7 .

Interestingly, RNA quantification performed in the spinal cord and dorsal root ganglia indicated important differences between the capsid variants. The spinal cord transduction profile was consistent with the brain, with a strong and consistent increase with TTD-003 and TTD-004 capsids, but interestingly the DRG transduction suggested a substantial detargeting of the TTD-004 capsid, whereas the TTD-003 capsid showed a strongly increased RNA expression as shown in FIG. 8 .

Total DNA was extracted from the same brain tissues as RNA, and biodistribution was measured by ddPCR using a Taqman set specific for the CMV promoter sequence. The RNAseP gene was used as a copy number reference. Vector genome (VG) per cell values were determined both by qPCR and ddPCR. Increased biodistribution was observed for the TTD-004 capsid in most brain regions, but surprisingly none of the other candidates showed a significant increase by comparison with AAV9. This apparent contradiction with the RNA quantification data could suggest that some capsids may present improved properties over AAV9 in post-attachment mechanisms rather than strict vector translocation in CNS parenchyma. Interestingly, DNA analysis confirmed the substantial detargeting of TTD-004 capsid from the DRG (FIG. 9A-9D).

To further explore the behavior of capsid variant TTD-004, viral genome (VG) quantification was completed from tissues collected from heart atrium, heart ventricle, quadriceps muscle, liver (left and right) and diaphragm and compared to vector genome presence as delivered by AAV9 in the same tissues. The data are shown in FIGS. 10A and B.

For TTD-003 and TTD-004 initial immunohistochemical analyses demonstrated the presence of payload-HA to a greater extent than seen with AAV9 delivery in cerebellar tissue, including in the dentate nucleus Immunohistochemistry confirmed the de-targeting of the dorsal root ganglia for capsid variant TTD-004 as compared to TTD-003 and AAV9.

Data for each of the variants were compiled as an average mRNA (fold over TBP) or DNA (VG per cell) quantification per capsid variant per tissue as shown in Table 8 below and FIG. 11A-11B and FIG. 12A-12B, respectively

TABLE 8 Characterization of exemplary capsid variants Measure Tissue AAV9 TTD-001 TTD-002 TTD-003 TTD-004 TTD-005 mRNA Frontal 0.000325065 2.7232575 0.000768179 0.006268831 0.007076252 0.002204024 Cortex mRNA Sensory 0.001486245 3.400055 0.00417739 0.006788644 0.010976612 0.004139604 Cortex mRNA Motor Cortex 0.00063318 9.00819 0.001050247 0.009954825 0.010522399 0.002942249 mRNA Putamen 0.000612759 3.557205 0.001395549 0.011832671 0.011476176 0.001150153 mRNA Thalamus 0.002610992 2.863635 0.013937891 0.101411445 0.07565653 0.01100289 mRNA Cerebellar 0.00133497 1.3439 0.008517779 0.006396677 0.012964181 0.004382119 Cortex mRNA Dentate 0.001364954 0.963955 — — — — Nucleus mRNA Caudate 0.000352281 1.3026 — 0.003259804 0.00634117 — mRNA Hippocampus 0.000311824 0.407015 — — — — mRNA SC-cervical 0.012205449 11.877762 0.022004264 0.026994764 0.088316491 0.005773054 mRNA SC-Thoracic 0.048833465 2.9974295 0.004360318 0.035118928 0.020543776 0.005629959 mRNA SC-Lumbar 0.029887407 7.969603 0.056231995 0.016033388 0.047713563 0.026324154 mRNA DRG- 0.74570895 9.274951 0.007897714 2.47872652 0.280868887 0.008122233 cervical mRNA DRG- 0.5559061 5.22606 0.006456564 8.721845271 0.104701895 — Thoracic mRNA DRG- 1.089758 17.308436 0.008247771 2.271300217 0.426704698 0.119974244 Lumbar mRNA Lung 0.004807149 0.000546842 — — 0.013744781 — mRNA Pancreas — — — — — — mRNA Colon 0.017962678 0.005041385 — — 0.183862903 — mRNA Kidney 0.043825993 0.006649157 — — 0.041234576 — mRNA Liver 0.674478605 0.253188648 — — 2.578654807 — mRNA Adrenal — — — — — — mRNA Spleen 0.014066875 0.000955981 — — 0.013435626 — mRNA Heart 1.323389668 0.132477314 — — 5.587929805 — mRNA Quadriceps 0.116623509 — — — 4.527799743 — mRNA Diaphragm 0.250001109 — — — 1.936435215 — DNA Frontal 0.07713 2.104843 0.10252 0.068367 0.380429 0.1257545 Cortex DNA Sensory 0.093003 2.679886 0.07443 0.034016 0.2670975 0.132503 Cortex DNA Motor Cortex 0.08796 4.3437625 0.0913085 0.094401 0.318999 0.1110695 DNA Putamen 0.0581365 3.07904 0.12326 0.1497635 0.2731175 0.0715295 DNA Thalamus 0.0524055 2.076863 0.0664225 0.090511 0.214999 0.086863 DNA Cerebellar 0.014238 0.186361 0.0092915 0.009578 0.0356345 0.0128655 Cortex DNA Dentate 0.025042 0.1861975 0.210238 0.041906 0.106107 0.055287 Nucleus DNA Caudate 0.079294 3.9433175 — 0.0529005 0.2451035 — DNA Hippocampus 0.095436 1.760891 0.205433 0.368645 1.335324 0.432829 DNA SC-cervical 0.0376 1.143863 0.061085 0.061535 0.07573 0.05885 DNA SC-Thoracic 0.02692 0.933734 0.025955 0.05011 0.064915 0.0355 DNA SC-Lumbar 0.03615 0.992728 0.019125 0.034175 0.085165 0.051475 DNA DRG- 0.0765 0.14319 0.08196 0.13722 0.04115 0.071625 cervical DNA DRG- 0.165865 0.172363 0.07202 0.133455 0.04444 0.03139 Thoracic DNA DRG- 0.218725 0.385712 0.146115 0.153205 0.032875 0.12034 Lumbar DNA Lung 1.085639916 3.72 0.958576278 0.700015423 1.22442329 0.919823152 DNA Pancreas 0.256670617 20.535 0.320558325 0.240633195 0.067860607 0.004802583 DNA Colon 0.053867646 3.405 1.179065405 0.348969617 0.116867365 0.015288464 DNA Kidney 0.896656371 26.635 4.861362029 0.532746958 0.386522209 7.973793288 DNA Liver 207.332334 217.64 111.910319 193.8349405 448.5980021 213.0317219 DNA Adrenal 1.647725996 0.69 1.561129869 1.871878 1.269473156 0.847293047 DNA Spleen 14.93815481 20.43565 51.70294001 22.79095714 6.514778227 45.91987284 DNA Heart 2.012377817 14.49 0.757528914 1.780956673 3.814571986 0.44694144 DNA Quadriceps 0.724278943 1.285 0.476250457 1.366015493 5.611203726 0.646197937 DNA Diaphragm — 1.06 — — — —

When calculated as fold over AAV9 the data were as shown in Table 9 below and FIG. 13A-13B and FIG. 14A-14B.

TABLE 9 Characterization of exemplary capsid variants Measure Tissue AAV9 TTD-001 TTD-002 TTD-003 TTD-004 TTD-005 mRNA Frontal Cortex 1.0 8378 2.4 19.3 21.8 6.8 mRNA Sensory Cortex 1.0 2288 2.8 4.6 7.4 2.8 mRNA Motor Cortex 1.0 14227 1.7 15.7 16.6 4.6 mRNA Putamen 1.0 5805 2.3 19.3 18.7 1.9 mRNA Thalamus 1.0 1097 5.3 38.8 29.0 4.2 mRNA Cerebellar 1.0 1007 6.4 4.8 9.7 3.3 Cortex mRNA Dentate 1.0 706 — — — — Nucleus mRNA Caudate 1.0 3698 — — — — mRNA Hippocampus 1.0 1305 — — — — mRNA SC-cervical 1.0 973 1.8 2.2 7.2 0.5 mRNA SC-Thoracic 1.0 61 0.1 0.7 0.4 0.1 mRNA SC-Lumbar 1.0 267 1.9 0.5 1.6 0.9 mRNA DRG-cervical 1.0 12 0.0 3.3 0.4 0.0 mRNA DRG-Thoracic 1.0 9 0.0 15.7 0.2 — mRNA DRG-Lumbar 1.0 16 0.0 2.1 0.4 0.1 mRNA Lung 1.0 0.11 — — 2.9 — mRNA Pancreas — — — — — — mRNA Colon 1.0 0.28 — — 10.2 — mRNA Kidney 1.0 0.15 — — 0.9 — mRNA Liver 1.0 0.38 — — 3.8 — mRNA Adrenal — — — — — — mRNA Spleen 1.0 0.07 — — 1.0 — mRNA Heart 1.0 0.10 — — 4.2 — mRNA Quadriceps 1.0 — — — 38.8 — mRNA Diaphragm 1.0 — — — 7.7 — DNA Frontal Cortex 1.0 27.29 1.3 0.9 4.9 1.6 DNA Sensory Cortex 1.0 28.82 0.8 0.4 2.9 1.4 DNA Motor Cortex 1.0 49.38 1.0 1.1 3.6 1.3 DNA Putamen 1.0 52.96 2.1 2.6 4.7 1.2 DNA Thalamus 1.0 39.63 1.3 1.7 4.1 1.7 DNA Cerebellar 1.0 13.09 0.7 0.7 2.5 0.9 Cortex DNA Dentate 1.0 7.44 8.4 1.7 4.2 2.2 Nucleus DNA Caudate 1.0 49.73 — 0.7 3.1 — DNA Hippocampus 1.0 18.45 2.2 3.9 14.0 4.5 DNA SC-cervical 1.0 30.42 1.6 1.6 2.0 1.6 DNA SC-Thoracic 1.0 34.69 1.0 1.9 2.4 1.3 DNA SC-Lumbar 1.0 27.46 0.5 0.9 2.4 1.4 DNA DRG-cervical 1.0 1.87 1.1 1.8 0.5 0.9 DNA DRG-Thoracic 1.0 1.04 0.4 0.8 0.3 0.2 DNA DRG-Lumbar 1.0 1.76 0.7 0.7 0.2 0.6 DNA Lung 1.0 3.43 0.9 0.6 1.1 0.8 DNA Pancreas 1.0 80.01 1.2 0.9 0.3 0.0 DNA Colon 1.0 63.21 21.9 6.5 2.2 0.3 DNA Kidney 1.0 29.70 5.4 0.6 0.4 8.9 DNA Liver 1.0 1.05 0.5 0.9 2.2 1.0 DNA Adrenal 1.0 0.42 0.9 1.1 0.8 0.5 DNA Spleen 1.0 1.37 3.5 1.5 0.4 3.1 DNA Heart 1.0 7.20 0.4 0.9 1.9 0.2 DNA Quadriceps 1.0 1.77 0.7 1.9 7.7 0.9 DNA Diaphragm — — — — — —

Capsid variant TTD-001 showed greater than 5,000 fold increase in payload-HA levels delivered to the brain as compared to AAV9 and measured by qRT-PCR and normalized to TBP. In all CNS tissues measured, TTD-001 showed dramatically enhanced delivery of payload-HA as compared to AAV9.

Graphical representations of the spinal cord and dorsal root ganglia measurements outlined in Tables 8 and 9 are shown in FIG. 15A-15B, FIG. 16A-16B, FIG. 17A-17B, and FIG. 18A-18B.

Immunohistochemistry of fixed brain tissues revealed dramatic transduction in both NHP tested by TTD-001 of the dentate nucleus, cerebellar cortex, cerebral cortex, brain stem, hippocampus, thalamus and putamen. AAV9 transduction of the dentate nucleus, cerebellar cortex, cerebral cortex, hippocampus, thalamus and putamen appeared negligible in comparison. TTD-001 therefore demonstrated broad and robust expression and distribution in the brain following intravenous administration in NHPs. In the dorsal root ganglia, both TTD-001 and AAV9 showed similar IHC patterns. Images of these stainings are shown in FIG. 19A-19E.

Immunohistochemical support for the DRG de-targeting nature of capsid variant TTD-004 (as noted above) is shown in FIG. 20A-20B.

Graphical representations of the biodistribution of viral genomes delivered by variant capsids or AAV9 to peripheral tissues is shown in FIG. 21A-21B.

Example 6. Individual Capsid Characterization in the Heart

This Example characterized the transduction level and the spatial distribution the TTD-001 (SEQ ID NO: 3623 and 3636, comprising targeting peptide SEQ ID NO: 1725 or 3648) and TTD-004 (SEQ ID NO: 3627 and 3639, comprising targeting peptide SEQ ID NO: 1760 or 3651) capsid variants in the heart muscle.

AAV particles were generated with each of a TTD-001 and TTD-004 capsid variant or a wild-type AAV9 capsid polypeptide control, encapsulating a transgene encoding a payload fused to an HA tag (payload-HA) and driven by a full-length CMV/chicken beta actin promoter. The AAV particles comprising the TTD-001 or TTD-004 capsid variant or the wild-type AAV9 capsid control, were administered intravenously to 2 female NHPs at a dose of 2e13 VG/kg. At day 14 post-administration of the AAV particles, the heart tissue was collected, fixed, and paraffin embedded for immunohistochemical staining. An anti-HA antibody (Cell Signal Technology) was used for staining the heart tissue for visualization of the transduction and distribution of the AAV capsid variants investigated. Both left and right heart ventricle samples were collected and analyzed.

As shown in FIG. 22A-22C, immunohistochemistry of the fixed heart tissue and cardiomyocytes, demonstrated transduction by both TTD-001 and TTD-004, in the left ventricle (FIG. 22B) and right ventricle (FIG. 22C). However, TTD-004 led to the greatest transduction in the left and right ventricle regions of the heart (FIG. 22B-22C), as compared to TTD-001 and the wild-type AAV9 control, as evidenced by increased IHC staining. TTD-001 and the wild-type AAV9 control demonstrated similar levels of transduction in both regions of the heart, as evidenced by similar IHC staining patterns. These data demonstrate that both the TTD-001 and TTD-004 capsid variants can be utilized to transduce and/or deliver a payload, e.g., a payload described herein, to a heart muscle. 

We claim:
 1. An adeno-associated virus (AAV) capsid variant comprising the amino acid sequence of PLNGAVHLY (SEQ ID NO: 3648) in hypervariable loop VIII, wherein the AAV capsid variant has increased tropism for brain cells or brain tissue as compared to a wild-type AAV9 capsid.
 2. The AAV capsid variant of claim 1, wherein the amino acid sequence of PLNGAVHLY (SEQ ID NO: 3648) is present immediately after amino acids 1-586 of the amino acid sequence of SEQ ID NO:
 3636. 3. An AAV particle comprising an AAV genome and the AAV capsid variant of claim
 2. 4. The AAV particle of claim 3, comprising a nucleotide sequence encoding a protein, an antibody, an enzyme, or an inhibitory RNA (iRNA), wherein the nucleotide sequence is operably linked to a promoter.
 5. The AAV particle of claim 4, wherein the protein is apolipoprotein E (APOE), human survival of motor neuron 1 (SMN1), human survival of motor neuron 2 (SMN2), aromatic L-amino acid decarboxylase (AADC), aspartoacylase (ASPA), tripeptidyl peptidase I, beta-galactosidase, N-sulphoglucosamine sulphohydrolase, N-acetyl-alpha-glucosaminidase (NAGLU), iduronate 2 sulfatase (IDS), intracellular cholesterol transporter (NCP1), or gigaxonin.
 6. The AAV particle of claim 4, wherein the enzyme is a meganuclease, a zinc finger nuclease, a transcription activator-like effector nuclease (TALEN), a recombinase, an integrase, or a Cas9.
 7. The AAV particle of claim 4, wherein the promoter is a human elongation factor 1α-subunit (EF1a), cytomegalovirus (CMV), CMV immediate-early enhancer (CMVIE), chicken β-actin (CBA), CAG, β glucuronidase (GUSB), ubiquitin C (UBC), neuron-specific enolase (NSE), platelet-derived growth factor (PDGF), platelet-derived growth factor B-chain (PDGF-8), intercellular adhesion molecule 2 (ICAM-2), synapsin (Syn), methyl-CpG binding protein 2 (MeCP2), Ca2+/calmodulin-dependent protein kinase II (CaMKII), metabotropic glutamate receptor 2 (mGluR2), neurofilament light (NFL), neurofilament heavy (NFH), p-globin minigene nβ2, preproenkephalin (PPE), enkephalin (Enk), excitatory amino acid transporter 2 (EAAT2), glial fibrillary acidic protein (GFAP), myelin basic protein (MBP), cardiovascular, liver, or skeletal muscle promoter.
 8. The AAV particle of claim 4, further comprising: (i) a polyA signal sequence; (ii) a 5′ inverted terminal repeat (ITR) and a 3′ ITR; (iii) an enhancer, a Kozak sequence, an intron, and/or an exon; (iv) at least one microRNA (miR) binding site; and/or (v) a nucleotide sequence encoding a Rep protein.
 9. The AAV particle of claim 8 comprising at least 2, 3, or 4 miR binding sites.
 10. The AAV particle of claim 8, wherein the miR binding site comprises a miR122, miR183, or miR142-3p binding site.
 11. A pharmaceutical composition comprising the AAV particle of claim 3, and a pharmaceutically acceptable excipient.
 12. The AAV particle of claim 3, wherein the AAV capsid variant has increased tropism for brain cells or brain tissue as compared to a wild-type AAV9 capsid when injected intravenously into a subject.
 13. The AAV particle of claim 12, wherein the AAV capsid variant has at least 5-fold increased tropism for brain cells or brain tissue as compared to a wild-type AAV9 capsid when injected intravenously into a subject.
 14. The AAV particle of claim 12, wherein the AAV capsid variant is capable of transducing the dentate nucleus, cerebellar cortex, cerebral cortex, brain stem, hippocampus, thalamus, or putamen of the subject.
 15. The AAV capsid variant of claim 1 comprising an amino acid sequence that is at least 95% identical to amino acids 203-743 of SEQ ID NO:
 3636. 16. The AAV capsid variant of claim 1 comprising an amino acid sequence that is at least 98% identical to amino acids 203-743 of SEQ ID NO:
 3636. 17. The AAV capsid variant of claim 1 comprising amino acids 203-743 of SEQ ID NO:
 3636. 18. The AAV capsid variant of claim 1 comprising an amino acid sequence that is at least 95% identical to amino acids 138-743 of SEQ ID NO:
 3636. 19. The AAV capsid variant of claim 1 comprising an amino acid sequence that is at least 98% identical to amino acids 138-743 of SEQ ID NO:
 3636. 20. The AAV capsid variant of claim 1 comprising amino acids 138-743 of SEQ ID NO:
 3636. 21. The AAV capsid variant of claim 1 comprising an amino acid sequence that is at least 95% identical to the amino acid sequence of SEQ ID NO:
 3636. 22. The AAV capsid variant of claim 1 comprising an amino acid sequence that is at least 98% identical to the amino acid sequence of SEQ ID NO:
 3636. 23. The AAV capsid variant of claim 1 comprising the amino acid sequence of SEQ ID NO:
 3636. 24. An isolated mammalian or insect cell comprising the AAV capsid variant of claim
 1. 25. The isolated mammalian or insect cell of 24, wherein the cell is: (i) a cell of the frontal cortex, sensory cortex, motor cortex, caudate, dentate nucleus, cerebral cortex, brain stem, hippocampus, thalamus, putamen, cervical spinal cord region, thoracic spinal cord region, lumbar spinal cord region, or a combination thereof; or (ii) a neuron, a sensory neuron, or a motor neuron. 